• Animal cells and systems
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• v.1 no.4
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• pp.595-602
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• 1997

Treatment of newborn female rats with gonadal steroids induces permanent sterility in adulthood. We investigated the alteration in expression patterns of gonadotropin-releasing hormone (GnRH) and luteinizing hormone (LH) in neonatally estrogenized sterile rats (ESR). Newborn female rats received daily injections of 17${\beta}$-estradiol (E, 10 ${\mu}$g) from the day of birth (day 1) to postnatal day 5. Controls were subjected to vehicles over the same period. All animals were sacrificed on week 7 after birth. Hypothalamic GnRH mANA levels were markedly higher in all ESR than in controls, while hypothalamic GnRH contents in ESR increased in proportion to the frequency of daily administration of E. However, both pituitary LH6 mRNA and serum LH levels were inversely decreased by the same treatment. The data indicate that neonatal exposure of E equally elevates the expression of GnRH gene, but reduces the secretion of GnRH, accordingly leading to attenuation of LH6 gene expression and circulating LH levels. The temporal effect of E and/or progesterone (P) on GnRH and LH6 mRNA levels was also examined in ESR. Newborn female rats were daily injected with E (10 ${\mu}$g) or vehicle for five successive days from day 1 and ovariectomized at week 5. They were implanted with E (235 ${\mu}$g/ml) two days prior to week 7, injected with P (1 mg) 42 h later, and sacrificed 7 h after P administration. In ovariectomized controls, hypothalamic GnRH mRNA levels were dropped to half by treatment of E and restored by subsequent treatment of P. The negative feedback action of E on GnRH mRNA levels observed in ovariectomized rats was completely blocked by neonatal exposure of E. The change in pituitary LH mRNA levels was similar to that in hypothalamic GnRH mRNA levels. Taken together, the results suggest that neonatal treatment of E alters the synthesis and release of GnRH in adulthood and furthermore blocks the negative feedback regulation of E which occurs normally after ovariectomy.

• Environmental and Resource Economics Review
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• v.31 no.4
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• pp.645-668
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• 2022

Hydrogen energy is emerging as an important means of carbon neutrality in the various sectors including power, transportation, storage, and industrial processes. Fuel cell power plants are the fastest spreading in the hydrogen ecosystem and are one of the key power sources among means of implementing carbon neutrality in 2050. However, high volatility in system marginal price (SMP) and renewable energy certificate (REC) prices, which affect the profits of fuel cell power plants, delay the investment timing and deployment. This study applied the real option methodology to analyze how the dual uncertainties in both SMP and REC prices affect the investment trigger price level in the irreversible investment decision of fuel cell power plants. The analysis is summarized into the following three. First, under the current Renewable Portfolio Standard (RPS), dual price uncertainties passed on to plant owners has significantly increased the investment trigger price relative to one under the deterministic price case. Second, reducing the volatility of REC price by half of the current level caused a significant drop in investment trigger prices and its investment trigger price is similar to one caused by offering one additional REC multiplier. Third, investment trigger price based on gray hydrogen and green hydrogen were analyzed along with the existing byproduct hydrogen-based fuel cells, and in the case of gray hydrogen, economic feasibility were narrowed significantly with green hydrogen when carbon costs were applied. The results of this study suggest that the current RPS system works as an obstacle to the deployment of fuel cell power plants, and policy that provides more stable revenue to plants is needed to build a more cost-effective and stable hydrogen ecosystem.

• ALGAE
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• v.32 no.2
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• pp.139-153
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• 2017

The ability of a red tide species to take up nutrients is a critical factor affecting its red tide dynamics and species competition. Nutrient uptake by red tide species has been conventionally measured by incubating nutrient-depleted cells for a short period at 1 or 2 light intensities. This method may be applicable to certain conditions under which cells remain in oligotrophic water for a long time and high nutrients are suddenly introduced. Thus, a new method should be developed that can be applicable to the conditions under which cells are maintained in eutrophicated waters in healthy conditions and experience light and dark cycles and different light intensities during vertical migration. In this study, a new repletion method reflecting these conditions was developed. The nitrate uptake rates of the red tide dinoflagellate Prorocentrum micans originally maintained in nitrate repletion and depletion conditions as a function of nitrate concentration were measured. With increasing light intensity from 10 to $100{\mu}E\;m^{-2}s^{-1}$, the maximum nitrate uptake rate ($V_{max}$) of P. micans increased from 3.6 to $10.8 pM\;cell^{-1}d^{-1}$ and the half saturation constant ($K_{s-NO3}$) increased from 4.1 to $6.9{\mu}M$. At $20{\mu}E\;m^{-2}s^{-1}$, the $V_{max}$ and $K_{s-NO3}$ of P. micans originally maintained in a nitrate repletion condition were similar to those maintained in a nitrate depletion condition. Thus, differences in cells under nutrient repletion and depletion conditions may not affect $K_{s-NO3}$ and $V_{max}$. Moreover, different light intensities may cause differences in the nitrate uptake of migratory phototrophic dinoflagellates.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.6 no.3
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• pp.427-433
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• 2002

This paper describes a design of cryptographic processor that implements the AES(Advanced Encryption Standard) block cipher algorithm "Rijndael". To achieve high throughput rate, a sub-pipeline stage is inserted into the round transformation block, resulting that the second half of current round function and the first half of next round function are being simultaneously operated. For area-efficient and low-power implementation, the round block is designed to share the hardware resources in encryption and decryption. An efficient scheme for on-the-fly key scheduling, which supports the three master-key lengths of 128-b/192-b/256-b, is devised to generate round keys in the first sub-pipeline stage of each round processing. The cryptoprocessor designed in Verilog-HDL was verified using Xilinx FPGA board and test system. The core synthesized using 0.35-${\mu}{\textrm}{m}$ CMOS cell library consists of about 25,000 gates. Simulation results show that it has a throughput of about 520-Mbits/sec with 220-MHz clock frequency at 2.5-V supply.-V supply.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.42 no.6
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• pp.674-682
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• 2009

Recently, the occurrence of harmful algae blooms from the Chattonella group has been increasing and expanding in the southern and western seas of Korea. We investigated the relationship between growth kinetics and nutrients in the harmful algae Chattonella marina and Chattonella ovata of the South Sea, Korea. As a result; high concentrations of ammonium ($30\;{\mu}M$ and above) was not effective to the growth of C. ovata, while C. marina displayed good growth at concentration of $100\;{\mu}M$. The half-saturation constant ($K_s$) of C. marina for ammonium ($2.51\;{\mu}M$), nitrate ($5.36\;{\mu}M$) and phosphate ($0.75\;{\mu}M$) was higher than C. ovata (1.85, 4.01, and $0.61\;{\mu}M$, respectively). This indicates that C. ovata can achieve higher cell densities than C. marina under lower nutrient conditions. These $K_s$ values were comparatively higher than those of diatoms and other flagellates previously reported. Therefore, our results indicate that the growth of C. marina and C. ovata is less adapted to lower nutrient environments than other competition species, such as Skeletonema costatum and Cochlodinium polykrikoides.

• Nuclear Engineering and Technology
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• v.20 no.3
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• pp.179-188
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• 1988

A recently developed spatial differencing scheme, Linear Characteristic (LC) scheme is compared with some traditionally used schemes such as Step Difference (SD), Diamond Difference (DD), and Step Characteristic (SC) scheme by analyzing the truncation error calculated numerically in slab geometry. Those four candidate schemes are applied to one simple source sink problem and two criticality problems (one is calculation of multiplication factor and the other is slab critical half thickness). The calculated results are then examined by some equitable measures of error. It is concluded that the LC scheme is terribly more powerful than any other candidate scheme that has been prevalent up to the present time. Moreover, the LC scheme estimates integral parameter such as multiplication factor and critical half thickness much more efficiently than SD or SC scheme. This is due to the fact that the fortuitous error cancellation, which occurs when the deviations of cell average flux are summed over the whole gamut of spatial meshes, happens much more favorably to the LC scheme.

• The Journal of the Institute of Internet, Broadcasting and Communication
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• v.8 no.4
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• pp.53-58
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• 2008

This paper designs sigma-delta D/A Converter with a high resolution and low power consumption. It reorganizes the input data along LJ, RJ, I2S mode and bit mode to the output data of A/D converter. The D/A converter decodes the original analog signal through HBF, Hold and 5th CIFB(Cascaded Integrators with distributed Feedback as well as distributed input coupling) sigma-delta modulation blocks. It uses repeatedly the addition operation in instead of the multiply operation for the chip area and the performance. Also, the half band filters of similar architecture composed the one block and it used the sample-hold block instead of the sinc filter. We supposed simple D/A Converter decreased in area. The filters of the block analyzed using the matlab tool. The top block designed using the top-down method by verilog language. The designed block is fabricated using Samsung 0.35um CMOS standard cell library. The chip area is 1500*1500um.

• The Journal of Korean Institute of Electromagnetic Engineering and Science
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• v.25 no.3
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• pp.339-347
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• 2014

In this paper, the authors present a new design for a broad-band metamaterial(MTM) absorber that utilizes flexible substrate. The proposed MTM unit cell is constructed by a electric-inducive-capacitive(ELC) resonator and a cut-wire on the same side of the flexible polyimide substrate. To reduce the radar cross section at frequencies other than the targeted frequency bands, the metallic pattern layer of the proposed structure is placed facing toward the incident wave propagation direction. A prototype absorber was fabricated with a planar array of $33{\times}45$ unit cells. Our experiments showed that the proposed absorber exhibits a peak absorption rate of 92 % and 93 % at 9.06 GHz and 15.0 GHz, respectively, and 75 % of the full-width at half-maximum(FWHM) bandwidth is achieved. The proposed backplane-less MTM structure can be used for a broad-band microwave absorber and irregular surface applications.

• Parasites, Hosts and Diseases
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• v.51 no.5
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• pp.503-510
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• 2013

Toxoplasma gondii is an apicomplexan parasite with a broad host range of most warm-blooded mammals including humans, of which one-thirds of the human population has been infected worldwide which can cause congenital defects, abortion, and neonatal complications. Here, we developed a rapid diagnostic test (RDT) for T. gondii infection. Antigenic N-terminal half of the major surface antigen (SAG1) was linked with intrinsically unstructured domain (IUD) of dense granule protein 2 (GRA2). The recombinant GST-GRA2-SAG1A protein was successfully expressed and purified as 51 kDa of molecular weight. Furthermore, antigenicity and solubility of the rGST-GRA2-SAG1A protein were significantly increased. The overall specificity and sensitivity of GST-GRA2-SAG1A loaded RDT (TgRDT) were estimated as 100% and 97.1% by comparing with ELISA result which uses T. gondii whole cell lysates as the antigen. The TgRDT tested with Uganda people sera for field trial and showed 31.9% of seroprevalence against T. gondii antibody. The TgRDT is proved to be a kit for rapid and easy to use with high accuracy, which would be a suitable serodiagnostic tool for toxoplasmosis.

• 한국해양학회지
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• v.19 no.2
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• pp.172-186
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• 1984

Phyroplankton samples were collected seasonally from March to December, 1982 in order to study the distribution of phyroplankton and their relation to environmental factors in Kwang-yang Bay. A total of 211 taxa of phyroplankton representing 67 genera, 196 species, 14 varieties, and 1 forma have been identified. Seasonal succession of dominant species is evident in this bay; Rhiwosolenia deiicatula, Chroomonas sp. being predominant in spring, Chroomonas sp. in summer, Chaetoceros debilis, C. socialis in autumn, and Skeletonema costatum, Chroomonas sp. in winter, repectively. The standing crops of the phyroplankton vary with time, and hare relevance to NH$\_$3/-N concentration, grazing pressure, and temperature. In spring, diatom blooming is relatively well correlated with the NH$\_$3/-N concentration. The species diversity is rather low in general. The fraction of nanoplankton total cell number represents an annual average of 36.5%, showing a meaningful contribution to the primary production and food web in this water system. Spatial distribution of phytoplankton indicates that this bay could e divided into three chracteristic areas; Seomjin-river mouth area heavily influenced by fresh water, western-half area scarecely affected by the oceanic water, and eastern-half area heavily influenced by the oceanic water. The industrial effluents from the YeochunIndustrial Complex also affected the spatial distribution of phyroplankton.