• 한국동물위생학회지
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• 제22권1호
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• pp.61-70
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• 1999

This study is concerned with assessment of diethylnitrosamine(DEN) induced liver cell carcinogenesis by measurement of changes preceding the development of neoplasms. Therefore, it was undertaken to investigate the changes of liver-specific enzyme activities in rats (Sprague-Dawley) by ad libitum feeding of DEN. And also, as another objective index in urine, the level of urinary biopterin was measured by high performance liquid chromatography(HPLC) method. The results were as follows ; 1. Minor behavioral change, brittleness of hair and decreased amount of water and diet intake were observed in rats 7 weeks after DEN administration. 2. The body and liver weights were significantly(p<0.05) decreased in rats 11 weeks after DEN administration. 3. The ratio of liver weight to body weight was rather stable and not significantly decreased in the all treatment groups. 4. The liver specific enzyme activities(AST, ALT,$\gamma$-GTP) were significantly(p<0.05) increased in all treatment groups compared to control group. 5. Compared to normal level, urine biopterin level was significantly (p<0.05) increased in all treatment groups(p<0.05). In conclusion, this result confirmed that the DEN was one of the potent hepatocarcinogens. In experimental model of rats exposed to DEN, the results indicated that values of liver specific enzyme activities(AST, ALT, $\gamma$-GTP) and urine biopterin level could be useful complementary tumor indices.

• 대한수의학회지
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• 제27권1호
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• pp.77-83
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• 1987

For the development of the specific pathogen free (SPF) or germ free laboratory animals, a parasitological approach was applied to the preliminarily selected laboratory albino mice (Mus musculus alba) in order to observe the ectoparasites on the hair of the host animal. The mites collected from the laboratory albino mice were identified as Myocoptes musculinus and classified into the Family Listrophoridae, Suborder Sarcoptiformes, Order Acarina. The overall infection rate of the mites was revealed as high as 73.2% (52 out of 71 heads) and the development process from the eggs to larvae was observed for the understanding of the basic ecological properties.

• 대한수의학회지
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• 제36권2호
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• pp.471-482
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• 1996

These experiments were undertaken in order to find out the useful clinicopathological diagnostic methods of cadmium poisoning in dogs. Twenty-one dogs were divided into a control group and 6 experimental groups. The experimental groups were adminstered orally 5, 10, 15, 30, 60 and 120mg of cadmium per kg of body weight for 56 days. All dogs were examined for clinical signs, and weekly changes in hematological and blood chemical values. All dogs were necropsied on 57th days of experiment. Tissue samples including hair, skin, muscle, lung, liver, kidney, spleen, pancreas, testis, ovary, uterus, and bone were collected and analyzed for cadmium, zinc, iron and copper contents using atomic absorption spectrophotometer. From these experiments following results were obtained : 1. All experimental dogs showed vomitting, salivation, anorexia, decreased water-intake, dehydration, and marked weight loss. The dogs received 30mg/kg or more of cadmium died during the period from 2nd to 7th week after administration. 2. Hematologically, all experimental dogs showed decrease in erythrocyte count, hemoglobin concentration, and packed cell volume. The anemia was identified as normocytic and regenerative morphologically. 3. No significant differences in serum glutamic oxaloacetic transminase, glutamic pyruvic transaminase, blood urea nitrogen, and cholosterol value were obseved between the control and experimental dogs. 4. The cadmium contents in various tissues of experimental dogs were estimated as $37.8{\sim}201.8{\mu}g/g$ in bone, $14.1{\sim}49.5{\mu}g/g$ in liver, $13.2{\sim}53.1{\mu}g/g$ in kidney, $0.4{\sim}35.2{\mu}g/g$ in pancreas, $0.8{\sim}35.4{\mu}g/g$ in spleen, $0.9{\sim}30.1{\mu}g/g$ in hair, $0{\sim}7.1{\mu}g/g$ in lung, $0{\sim}5.1{\mu}g/g$ in skin, and $0{\sim}3.6{\mu}g/g$ in muscle, respectively. However, the serum, testis, ovary and uterus showed no cadmium accumulation. Two contol dogs showed cadmium accumulation only in bone. 5. Significant differances in zinc, iron, and copper contents in tissue samples were observed between the control and experimental groups.

• 대한본초학회지
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• 제32권5호
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• pp.19-25
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• 2017

Objective : The Sinseon-yukza-hwan (SSY), a Korean medicinal formula which includes Radix Rehmanniae Preparata and other medicinal herbs, has long been used for treatment of alopecia and gray hair through oral administration. This study is designed to enhance the utilization of natural materials in hair and scalp-related cosmetics. Possibility of SSY as an antioxidant was examined from its 50% ethanol extract. Methods : The antioxidative capacities were evaluated by determining total phenolic and flavonoid contents, 1,1-diphenyl-2-picryl hydrazyl (DPPH), 2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), reducing power and hydroxyl radical scavenging activity. Results : Total polyphenol and flavonoid contents of SSY were 25.53 mg TE, tannic acid equivalent/g and 18.90 mg RE, rutin equivalent/g, respectively, which correlated strongly its antioxidative activity. The DPPH and ABTS free radical scavenging activities of SSY at $0.1mg/m{\ell}{\sim}5mg/ml$ were ranged from 20% to 85% and 10% to 58%, respectively. Also the hydroxyl radical scavenger activity and reducing power increased in SSY-treated group, which were significantly lower in SSY-compared to BHA-treated group. But the highest reducing power was shown as 79% from SSY-treated group, which was higher value than 65% from BHA-treatment. These results showed that SSY extract effectively inhibited the generation of free radicals in the all assay system with dose-dependent manners. Conclusions : Thus, the present study provide preclinical data to support the expanded application of SSY, which could be potential candidates for natural antioxidants.

• 한국식품영양과학회:학술대회논문집
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• 한국식품영양과학회 2001년도 International Symposium on Food,Nutrition and Health for 21st Century
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• pp.69-73
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• 2001

Two opioid peptides, YPLDL and YPLDLF, were isolated from enzymatic digests of spinach ribulose-1, 5-bisphosphate carboxylase/oxygenase (RuBisCO) and named rubiscolin-5 and -6, respectively. These peptides were selective for delta-receptor and the latter was about 3 times more potent than the former. After oral administration in mice at the dose of 100 mg/kg, rubiscolin-6 showed analgesic activity in tail pinch test. It also stimutated learning performance at the same dose in passive avoidance experiment using step-through apparatus. An immunostimulating peptide, MITLAIPVNKPGR, was isolated from a trypsin digest of soybean protein and named soymetide. Immunostimulating activy of soymetide was mediated by fMLP receptor. Interestingly, after oral administration in rats at a dose of 300 mg/kg (po.), soymetide-4 (MITL) protected alopecia (hair-loss) induced by etoposide, a cancer chemotherapy agent. Stimulation of IL-1 release by the peptide was involved in the mechanism. Ovokinin(2-7), RADHPF, is a vasorelaxing peptide released from ovalbumin by the action of chymotrypsin. It lowered blood pressure of spontaneously hypersensive rats (SHR) after oral administration at a dose of 10 mg/kg. RPLKPW, which was designed by replacing 4 amino acid residues in ovokinin(2-7), exhibited hypotensive activity at a dose of 0.1 mg/kg (po.). This peptides was introduced into 3 homologous sites in soybean beta-conglycinin alpha' subunit by site-directed mutagenesis of the cDNA and expressed in E. coli. The minimum effective dose for hypotensive activity of the genetically modified beta-conglycinin alpha' subunit was 10 mg/kg (po.), which is about 1/200 that of ovalbumin.

• Journal of Animal Science and Technology
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• 제65권5호
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• pp.989-1001
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• 2023

The study evaluated the effects of dietary fiber and energy levels administered during two growing periods (d 0-28 and d 29-56) for pigs exposed to a high temperature. A total of 96 growing pigs were used in six treatments as: Two treatments in thermoneutral temperature (21℃-24℃) with dietary energy of 3,300 and the inclusion of high or low fiber, two treatments in heat stress (30℃-34℃) with dietary energy of 3,300 and the inclusion of high or low fiber, and two treatments in heat stress with dietary energy of 3,450 and the inclusion of high or low fiber. Among standard energy level treatments, heat-stressed pigs showed lower average daily gain (ADG), feed intake, digestibility of dry matter, gross energy, crude protein, and crude fiber in phases 1 and 2. Moreover, higher concentrations of acetate, propionate, butyrate, and total short-chain fatty acid (SCFA) in feces were shown in pigs fed high fiber diets. There was a negative interaction between dietary fiber and energy for the fecal concentration of isobutyrate in phase 1 and valerate in phase 2. Pigs in heat stress treatments showed a higher rectal temperature, respiratory rate, hair cortisol, plasma zonulin, and fecal lipocalin-2. Among heat stress treatments, the overall ADG was increased in pigs fed high fiber. Pigs fed high dietary fiber showed a greater concentration of acetate, propionate, butyrate, and total SCFA. High fiber treatments decreased plasma zonulin. In conclusion, the inclusion of beet pulp, soluble fiber, at the level of 4% looks necessary in pigs diet during heat stress.

• 한국응용곤충학회지
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• 제26권4호
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• pp.245-249
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• 1987

사과품종별(品種別) 사과나무 반점낙엽병(斑點落葉病)의 발병(發病)과 잎의 모용(毛茸) 발생정도(發生程度)를 조사(調査)하고 사과나무 잎의 부위별(部位別) A. mali의 침입정도(浸入程度)와 모용(毛茸)이 병원균(病原菌)의 침입(浸入)과 감염(感染)에 미치는 영향(影響)을 조사(調査)한 결과(結果)는 다음과 같다. A. mali의 이병정도(罹病程度)에 따른 사과나무 품종(品種)의 분포(分布)는 이병엽율(罹病葉率)이 50.1% 이상(以上)되는 이병성품종(罹病性品種)을 조사(調査)한 82품종(品種) 중(中) 15개품종(個品種)(18.3%) 이었으며 주요(主要) 품종(品種)은 나리탄, 인도(印度), 왕령(王令), 스라크림숀, 스카이스파, 아오리 3호 등(等)이었고 이병엽율(罹病葉率)이 50% 이하(以下)되는 저항성품종(抵抗性品種)이 51개품종(個品種)(62.2%)이었는데 주요품종(主要品種)은 홍옥(紅玉), 무쓰, 모리쓰, 조나골드, 쓰가루, 가라, 골덴, 후지, 스파어리브레이즈 등(等)이었으며, 주요품종(主要品種)들의 엽당병반수(葉當病斑數)도 저항성품종(抵抗性品種)보다 이병성품종(罹病性品種)에서 많았다. 이병성품종군중(罹病性品種群中)에서 이병엽율(罹病葉率)이 6월(月)에 20%, 이상(以上)되어 8월(月)에 70% 이상(以上)되는 품종(品種)이 와인삽, 아오리 3호, 스카이스파, 왕령(王令) 등(等)과 6월(月)에는 5% 이상(以上) 되었다가 8월(月)에 70% 이상(以上)되는 품종(品種)이 라리단, 스타크림숀, 인도(印度) 등(等)으로 구분(區分)되었다. 품종별(品種別) 잎에 모용발생정도(毛茸發生程度)는 이병성(罹病性) 품종군(品種群)에서 보다 저항성품종군(抵抗性品種群)에서 대체(大體)로 많았고 동일(同一) 품종(品種)잎에서 모용무제거부위(毛茸無除去部位)보다 모용제거부위(毛茸除去部位)에서 감염율(感染率)이 높은 것으로 보아 잎의 모용(毛茸)은 품종(品種) 저항성(抵抗性) 요인(要因)으로 추정(推定)되었으며 A. mali의 침입(浸入)과 감염(感染)은 잎의 표면(表面)보다 이면(裏面)에서 용이(容易)하였다.

• 한국의류산업학회지
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• 제15권4호
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• pp.554-564
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• 2013

We provide an empirical assessment that examines the differences in appearance management behavior, life satisfaction and demographic variables between groups classified by individual lifestyle. Questionnaires were administered to 513 female and male adults over 17 years of age in the Daegu and Kyungbok metropolitan regions. Descriptive statistics, cluster analysis, Cronbach's ${\alpha}$, ANOVA, Duncan test and ${\chi}^2$ test were applied to analyze data from 513 respondents. The results are as follows. First, we did a cluster analysis on the appearance management behavior of weight training, skin care, hair care, make-up and clothing selection. Four groups (passive, rational, fashion oriented, and active typed) where classified according to individual lifestyle. Second, the rational and active groups were more interested in the social life, environmental stability, health, fashion and economic seeking life. They were also more involved in appearance management behavior and in a higher level of life satisfaction. However, the differences of life satisfaction among the lifestyle group (male) were not statistically significant. Third, females with higher level of income and education (among the demographic variables) belonged to the active group. We found significant differences in appearance management behavior, life satisfaction and demographic variables among male and female groups classified by lifestyle.

• 한국임상수의학회지
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• 제34권6호
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• pp.459-462
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• 2017

A 3-year-old, 5.6 kg, castrated, male, British short hair cat presented with subtle weight-bearing lameness of the right hindlimb when walking suddenly after rest. On physical examination, the patient experienced pain when the right stifle joint was hyperextended. There was no clinical improvement of lameness or pain of the right hindlimb, despite the administration of analgesic drugs for one week. During diagnostic arthroscopy of right stifle, mild synovitis and cranial cruciate ligament (CCL) impingement on osteophyte of the intercondylar notch (ICN) was observed. Computed tomography was applied to determine an accurate surgical plan. Arthroscopy-assisted notchplasty, including removal of the osteophyte, was performed using chisel and arthroscopic burr. At 4 weeks postoperatively, the frequency of subtle weight-bearing lameness of the right hindlimb was significantly reduced. At 6 months postoperatively, the patient exhibited normal ambulation without recurrence of lameness. Impingement of CCL may cause subtle lameness and pain. Arthroscopy can be a good diagnostic tool for early-stage CCL disease. Notchplasty can be recommended for osteophyte-induced CCL disease.

• 한국임상수의학회지
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• 제14권1호
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• pp.112-117
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• 1997

12-month-old Buldig and 3-year-old German Shepherd, treated with corticosteroids duringthe late pregnancy, were referred to the Veterinary Teaching Hospital, Chonnam National University, with chief complaints of vaginal discharge. Radiographic, ultrasonographic and surgical findings that obtained from each cases were as follows; case I: Radiographic findings were identified 2 fetuses that were normal skeleton, however, fetal viability could not be determined. Ultrasonographic findings were identified absence of fetal heartbeat and fetal movement, abnormal echogenicity of the abdominal visera, and echogenic materials remaining within the fetal fluid. After hysterotomy, macroscopic findings of uterus were liquefied materials remaining within the uterine lumen. Fetuses were excessively autolysis (falling off the hair, depression of the eye, and necrosis of the abdormal visera and skin). case II: Radiographic findings were identified irregular fetal structures and fetal movement, loss of recognizable fetal anatomy, dislocation of fetal veterbrae and echogenic materials remaining within the uterine lumen. After ovariohysterectomy, macroscopic finding of uterus and fetus were thickened uterine wall, yellowish-brown colored administration of corticosteroids during gestation should be contraindicated because fetal death is inducced. Ultrasonography has an advantage over radiography in that it permits evaluation of fetal viability.