The Korea Journal of Herbology (대한본초학회지)

The Korea Association of Herbology (대한본초학회)
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Antioxidant activities of ethanol extract of Shinsun-yukza-hwan, a Korean medicinal recipe

신선육자환 에탄올 추출물의 항산화 활성

  • Kim, Ji Yoon (Course of Beutytechnology, Center for Continuing Education, Dongnam Health University) ;
  • Park, Hae-Jin (Department of Herbal Pharmacology, Daegu Hanny University) ;
  • Kim, Mi Ryeo (Department of Herbal Pharmacology, Daegu Hanny University)
  • 김지윤 (동남보건대학교 평생교육원 뷰티테크놀리지과정) ;
  • 박해진 (대구한의대학교 한의과대학 본초약리학교실) ;
  • 김미려 (대구한의대학교 한의과대학 본초약리학교실)
  • Received : 2017.08.13
  • Accepted : 2017.09.15
  • Published : 2017.09.30
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Abstract

Objective : The Sinseon-yukza-hwan (SSY), a Korean medicinal formula which includes Radix Rehmanniae Preparata and other medicinal herbs, has long been used for treatment of alopecia and gray hair through oral administration. This study is designed to enhance the utilization of natural materials in hair and scalp-related cosmetics. Possibility of SSY as an antioxidant was examined from its 50% ethanol extract. Methods : The antioxidative capacities were evaluated by determining total phenolic and flavonoid contents, 1,1-diphenyl-2-picryl hydrazyl (DPPH), 2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), reducing power and hydroxyl radical scavenging activity. Results : Total polyphenol and flavonoid contents of SSY were 25.53 mg TE, tannic acid equivalent/g and 18.90 mg RE, rutin equivalent/g, respectively, which correlated strongly its antioxidative activity. The DPPH and ABTS free radical scavenging activities of SSY at $0.1mg/m{\ell}{\sim}5mg/ml$ were ranged from 20% to 85% and 10% to 58%, respectively. Also the hydroxyl radical scavenger activity and reducing power increased in SSY-treated group, which were significantly lower in SSY-compared to BHA-treated group. But the highest reducing power was shown as 79% from SSY-treated group, which was higher value than 65% from BHA-treatment. These results showed that SSY extract effectively inhibited the generation of free radicals in the all assay system with dose-dependent manners. Conclusions : Thus, the present study provide preclinical data to support the expanded application of SSY, which could be potential candidates for natural antioxidants.

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References

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