• 제목/요약/키워드: growth regulator

검색결과 606건 처리시간 0.022초

Growth Performance of Transgenic Mud Loach Misgurnus mizolepis Carrying a GH Transgene Driven by Mud Loach C-Type Lectin Regulator

  • Song, Ha-Yeon;Kim, Dong-Soo
    • Fisheries and Aquatic Sciences
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    • 제15권1호
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    • pp.43-47
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    • 2012
  • Growth hormone (GH) transgenesis in fish has the potential to improve aquaculture efficiency and capacity. However, many fast-growing transgenic fish have experienced side effects caused by excess GH expression. To overcome this unwanted issue associated with several GH transgenic mud loach Misgurnus mizolepis lines carrying GH construct driven by a strong ${\beta}$-actin regulator ($pml{\beta}$-actGH), we performed an alternative version of GH autotransgenesis using a weaker but more stable regulator, the mud loach lectin promoter. GH transgenesis with a pmlectGH construct consisting of the mud loach GH gene driven by the 2.3-kb lectin promoter exhibited significant growth stimulation. However, the extent of the growth acceleration in pmlectGH transgenics (six times maximum when assessed 2 months post hatching) was much less than that in transgenic individuals carrying the $pml{\beta}$-actGH construct. Additionally, the extraordinary gigantism that was common in $pml{\beta}$-actGH-transgenic mud loaches was diminished in transgenic loaches harboring the pmlectGH construct. Transgenic founders (pmlectGH) successfully transmitted their transgene into the next generation with up to 41% frequency. Growth stimulation also persisted in the transgenic F1 strains, with a seven-fold increase in maximum body weight at 6 months of age.

식물세포(植物細胞) 배양(培養)에 의(依)한 이차대사산물(二次代謝産物)의 생성(生成)에 관(關)한 연구(硏究) - II. Capsicum annuum L.의 배양세포(培養細胞)에 있어서 Growth Regulator가 Capsaicinoids, Phenylpropanoids 생성(生成) 및 Phenylalanine Amnonia-lyase (PAL) 활성(活性)에 미치는 영향 - (Formation of Secondary Products by Plant Cell Culture - II. Effects of Growth Regulators on the Formation of Capsaicinoide, Phenylpropanoids and PAL Activity in Cultured Cell of Capsicum annuum L. -)

  • 최봉순
    • 한국식품영양과학회지
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    • 제16권1호
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    • pp.10-17
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    • 1987
  • 고추의 배양세포(培養細胞)에 있어서 고추의 신미성분(辛味成分)인 capsaicinoids 생성(生成)에 미치는 growth regulator의 영향을 조사하기 위하며 growth regulator들을 각각 함유한 Linsmaier-Skoog 기본배지에서 배양(培養)한 callus의 capsaicinoids을 GLC에 의하여 조사하고 또한 capsaicinoids의 중간대사 산물인 phenylpropanoids와 phenylalanine ammonia-lyase activity을 측정하여 다음과 같은 결과를 얻었다. 1. Growth regulators가 배양세포(培養細胞)의 capsaicinoids 생성(生成)에 미치는 영향을 조사한 결과 growth regulators을 달리한 배지(培地)에서 생성(生成)된 capsaicinoids의 함량(含量)은 $2,4-D\;10^{-6}M$에서 가장 capsaicinoids의 생성량(生成量)이 많았으며 $IAA\;10^{-6}M,\;NAA\;10^{-6}M,\;kinetin\;10^{-6}M$의 배지(培地)에서는 2,4-D에 비(比)하여 각각 65%, 38%, 68%이었다. 2. Growth regulators을 달리한 기본배지에서 생성(生成)된 phenylpropanoids는 cinnamic acid, coumaric acid의 생성(生成)은 5, 15, 25일(日)에 점진적으로 증가하였으며 NAA 함유 배지에서는 2,4-D에 비해 약 30%, kinetin, IAA 함유배지에서는 약 70% 생성(生成)되었다. 3. $2,4-B\;10^{-6}M$을 함유한 배양세포(培養細胞)에서 phenylalanine ammonia-lyase activity을 측정한 결과 배양후(培養後) 5, 15, 25일(日) 배양일수에 따라 phenylalanine ammonia-lyase activity는 신선중량 g당 생성된 cinnamate는 각각 52, 81 및 209 % nmole로 증가하였다.

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Benzaldehyde as a new class plant growth regulator on Brassica campestris

  • Choi, Geun-Hyoung;Ro, Jin-Ho;Park, Byoung-Jun;Lee, Deuk-Yeong;Cheong, Mi-Sun;Lee, Dong-Yeol;Seo, Woo-Duck;Kim, Jin Hyo
    • Journal of Applied Biological Chemistry
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    • 제59권2호
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    • pp.159-164
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    • 2016
  • Plant growth regulator is an essential pesticide to date while the available active ingredient is not well understood unlike fungicide, insecticide and herbicide. This study was aimed to evaluate a new chemical class of plant growth regulator, and the total of 92 benzene derivatives were screened for their germination and early stage of the root growth regulation on Brassica campestris. Thirty benzaldehydes, nine acids, one amide, and one ester showed potent root growth inhibitory activity (>70 % inhibition) while only salicylaldehyde showed potent germination inhibition ($IC_{50}=81.2mg/L$) suggesting that benzaldehyde was a key module candidate for the growth inhibition. Benzaldehydes were further evaluated for root growth inhibition. 2,3-Dihydroxybenzaldehyde and salicylaldehyde showed $IC_{50}$ values of 8.0 and 83.9 mg/L, respectively. On the other hand, salicylaldehyde, and 2,4,5-trihydroxybenzaldehyde were found to have root growth promotion effects less than 10 mg/L. This result suggests that the benzaldehyde is a new class candidate for plant growth regulator.

상토 및 생장조절제 처리가 꾸지뽕나무의 삽목번식에 미치는 영향 (Effects of Bedsoil and Growth Regulator on Cutting Propagation of Cudrania tricuspidata Bureau)

  • 김인재;김민자;남상영;이철희;김홍식
    • 한국약용작물학회지
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    • 제12권4호
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    • pp.285-288
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    • 2004
  • 꾸지뽕나무의 대량번식을 위한 상토와 생장조절제 처리에 따른 삽목 번식에 관한 시험을 수행한 바, 삽목상토는 미풍화토에서 양호하여 숙지삽과 녹지삽에서 각각 56.9%, 62.3%의 발근율을 보였고, 생장조절제는 IBA 100 ppm처리에서 발근이 양호하여 숙지삽에서 57.9%, 녹지삽에서는 89.2%의 발근율을 보였다.

무늬발게 幼生에 對한 Dimilin 의 致死 影響 (Effects of the Insect Growth Regulator Dimilin on Larval Development of Hemigrapsus sanguineus (Crustacea, Brachyura) Reared in the Laboratory)

  • Kim, Chang Hyun
    • The Korean Journal of Ecology
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    • 제13권1호
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    • pp.19-24
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    • 1990
  • The effects of insect growth regulator Dimilin which interfere with the synthesis of chitin in the cuticle of insect larvae were investigated at various concentrations using the crab larvae of Hemigrapsus sanguineus. The larvae were cultured at control, 0.5, 1, 5 and 10 $\mu g$ Dimilin solutions and three replicate experiments were carried out to give correct analysis. Significant differences in percent mortality have occurred between control and 10$\mu g$ when the larvae were exposed to Dimilin whereas no differences were found between 5 and 10$\mu g$ Dimilin concentrations. If lethal concentration is defined as concentration at which less than 10 percent of crab larvae reach to the last zoeal stage from hatching it can be concluded that insect growth regulator Dimilin is lethal to the larvae of Hemigrapsus sanguineus at 5 and 10$\mu g$ Dimilin.

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Plant Growth Regulator Produced by Streptomyces sp. (Part II) Conditions of Production and Some Properties of the Plant Growth Regulator

  • 김광현;서정훈
    • 한국미생물생명공학회:학술대회논문집
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    • 한국미생물생명공학회 1978년도 추계학술대회
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    • pp.207.5-208
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    • 1978
  • Effects of the plant growth regulator (P. G. R.)on the reaction of proteinase, $\gamma-amylase$ and acid phosphatase were investigated, and also were the conditions of production of P. G. R. by Stroptomyces sp. 445. The P. G. R. had no effect on the act ivities of such enzymes in mung bean seedling. But in germinating seed previously treated with P. G. R. it effected the activity of protease in cotyledon. In the conditions of production of P. G. R., the maxim, activity was appeared in shaking cutlure at $30^{\circ}C$ for 5 days, and by the addition of peptone or casein hydrolysate as nitrogen source, soluble starch as carbon source, and sulfur as metal ion.

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BolA Affects Cell Growth, and Binds to the Promoters of Penicillin-Binding Proteins 5 and 6 and Regulates Their Expression

  • Guinote, Ines Batista;Matos, Rute Goncalves;Freire, Patrick;Arraiano, Cecilia Maria
    • Journal of Microbiology and Biotechnology
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    • 제21권3호
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    • pp.243-251
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    • 2011
  • The gene bolA was discovered in the 80's, but unraveling its function in the cell has proven to be a complex task. The BolA protein has pleiotropic effects over cell physiology, altering growth and morphology, inducing biofilm formation, and regulating the balance of several membrane proteins. Recently, BolA was shown to be a transcription factor by repressing the expression of the mreB gene. The present report shows that BolA is a transcriptional regulator of the dacA and dacC genes, thus regulating both DD-carboxypeptidases PBP5 and PBP6 and thereby demonstrating the versatility of BolA as a cellular regulator. In this work, we also demonstrate that reduction of cell growth and survival can be connected to the overexpression of the bolA gene in different E. coli backgrounds, particularly in the exponential growth phase. The most interesting finding is that overproduction of BolA affects bacterial growth differently depending on whether the cells were inoculated directly from a plate culture or from an overnight batch culture. This strengthens the idea that BolA can be engaged in the coordination of genes that adapt the cell physiology in order to enhance cell adaptation and survival under stress conditions.

식물 生長調整劑處理가 양파(Allium cepa L.)의 幼苗生長에 미치는 영향 (Effects of Plant Growth Regulator on Seedling Growth in Onion Seed(Allium cepa L.))

  • 이성춘
    • 한국자원식물학회지
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    • 제14권1호
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    • pp.38-42
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    • 2001
  • 양파의 직파재배를 안정화를 기하기 위한 일환으로 농도별로 조정한 생장조정제에 종자를 침지한 다음 포장에 파종하여 유묘의 초기생장성을 조사하였던 결과를 요약하면 다음과 같다. 1 식물생장조정제 처리 종자의 출아율은 BA, GA$_3$ 및 kinetin 이 각각 93.0, 94.3, 93.8%로 생장조정제별 차이가 거의 없었으나, 무처리 종자 80%보다는 훨씬 높았다. 2. 초장은 식물생장조정제 처리효과가 뚜렷하였는데, 그 정도는 GA$_3$, kinetin 및 BA순이었으며, GA$_3$,와 BA는 처리농도가 높을수록 초장이 컸던 반면, kinetin은 오히려 작아졌다. 3. 전개엽수는 식물생장조정제처리에 의해서 증가하였는데 식물생장제별 큰 차이는 없었다. 4. 유묘의 뿌리수는 식물생장조정제처리에 의해 뚜렷하게 증가하였는데. CA$_3$처리에서 가장 효과가 컸으며. 다음이 BA, kinetin 순이었다.

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Trinexapac-ethyl, Amidochlor 및 Mefluidide가 들잔디 직립생장에 미치는 효과 (Vertical Shoot Growth of Korean Lawngrass (Zoysia japonica Steud.) Influenced by Trinexapac-ethyl, Amidochlor, and Mefluidide)

  • 김경남;김용선
    • 원예과학기술지
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    • 제17권5호
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    • pp.572-577
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    • 1999
  • 여러 가지 생장조절제가 들잔디 직립생장에 미치는 영향을 규명함으로써 예초관리에 적절한 생장조절제 종류 및 적용수준을 파악하고자 본 연구를 수행하였다. 실험은 페어웨이 수준으로 유지되고 있는 들잔디 연구포장에서 1995년 2회에 걸쳐 각각 다른 장소에서 실시하였다. 공시한 3종류 생장조절제 모두 들잔디 생장을 억제하였지만, 억제기간은 종류에 따라 다르게 나타났다. 처리 1주 후 대부분의 생장조절제 처리구에서 약 10%에서 20% 정도의 억제효과가 있었다. Amidochlor 처리구는 $0.30mL{\cdot}m^{-2}$에서 $0.60mL{\cdot}m^{-2}$ 사이까지 3, 4주 정도 효과가 지속되었고, $0.08mL{\cdot}m^{-2}$ 이상의 trinexapacethyl 처리구에서는 8주까지 약 35% 정도의 억제효과가 관찰되었다. 다른 생장조절제에 비해 생장억제가 3, 4일 정도 빨리 나타난 mefluidide 처리구도 8주 정도 억제효과가 있었지만, 엽색 퇴화도 동시에 관찰되었다. 직립생장억제 관점에서 관리 정도가 낮게 유지되는 한국잔디에서 장기간 효과는 trinexapac-ethyl 처리가 amidochlor 및 mefluidide 보다 더 효과적인 것으로 사료되었다. 계절에 따라 생장조절제의 효과차이가 다르게 나타났는데, 들잔디 생육왕성기인 7월 처리에 비해 6월 처리시 직립경 생장억제 정도가 높게 나타났다. 본 연구를 통해 잔디 관리시 생장조절제 종류에 따라 30%에서 60% 정도 예초 회수를 감소시킬 수 있는 것으로 나타났으나, 예초 관리와 관련하여 기대하는 생장억제 기간에 따라 생장조절제 종류 및 적용 수준을 선택하는 것이 필요하다.

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수선의 기내 구 비대에 미치는 생장조절제와 저온처리의 영향 (Effect of Growth Regulators and Chilling Treatment on Bulblet Increment of Narcissus pseudo-narcissus 'King Alfred' in Vitro)

  • 정향영;신학기;김의영
    • 식물조직배양학회지
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    • 제24권2호
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    • pp.99-101
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    • 1997
  • 나팔수선 킹알프레드의 화경배양에서 얻은 대량의 소자구를 비대시키기에 적합한 호르몬 농도, 저온처리기간, 저온 처리 후 구비대에 적합한 호르몬 농도를 구명코자 하였다. 분화된 식물체의 구비대 및 생육은 BA 2.5mg/L에 NAA 2.5mg/L이 첨가된 MS배지에서 높았다. 자구비대를 위한 저온처리 기간은 8주간이 좋았으며 저온처리후 배양은 NAA 1mg/L가 첨가된 배지가 구비대에 가장 효과적이었다.

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