• Title/Summary/Keyword: growth behavior

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Isolation and Characterization of Lactic Acid Bacteria from Fermented Goat Milk in Tajikistan

  • Cho, Gyu-Sung;Cappello, Claudia;Schrader, Katrin;Fagbemigun, Olakunle;Oguntoyinbo, Folarin A.;Csovcsics, Claudia;Rosch, Niels;Kabisch, Jan;Neve, Horst;Bockelmann, Wilhelm;Briviba, Karlis;Modesto, Monica;Cilli, Elisabetta;Mattarelli, Paola;Franz, Charles M.A.P
    • Journal of Microbiology and Biotechnology
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    • v.28 no.11
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    • pp.1834-1845
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    • 2018
  • The lactobacilli associated with a fermented goat milk product from Tajikistan were isolated to characterize their technological properties and antibiotic resistances in order to assess their suitability for development as starter cultures. In this study, twenty three strains were identified by 16S rRNA sequencing as typical dairy-associated lactic acid bacterial strains, i.e. L. plantarum, L. pentosus, L. delbrueckii, L. helveticus and L. paracasei. These strains were generally susceptible to most antibiotics tested in this study and this allowed a selection of strains as safe starters. The draft genomes of four representative strains were sequenced and the number of contigs of the four assembled genomes ranged from 51 to 245 and the genome sizes ranged from 1.75 to 3.24 Mbp. These representative strains showed differences in their growth behavior and pH-reducing abilities in in vitro studies. The co-inoculation of these Lactobacillus spp. strains together with a yeast Kluyveromyces marxianus MBT-5698, or together with the yeast and an additional Streptococcus thermophilus MBT-2, led to a pH reduction to 3.4 after 48 h. Only in the case of fermentation inoculated with the co-culture, the viscosity of the milk increased noticeably. In contrast, fermentations with single strains did not lead to gelation of the milk or to a decrease in the pH after 24h. The results of this study provide a comprehensive understanding of the predominant lactobacilli related to Tajikistani fermented milk products.

Metal Corrosion Mechanism by Sulfate-reducing and Iron-oxidizing Bacteria in Saline System and its Optimal Inactivation (염수계 철산화균 및 황환원균에 의한 금속 부식 및 최적 제어 방안)

  • Sung, Eun-Hae;Han, Ji-Sun;Kim, Chang-Gyun
    • Journal of Korean Society of Environmental Engineers
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    • v.30 no.8
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    • pp.798-807
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    • 2008
  • Due to economic impairment derived from metal corrosion of pumping station installed around coastal area, it was needed for related cause-effect to be investigated for understanding practical corrosion behavior and providing proper control. This research was thus carried out to determine whether the microbe can influence on metal corrosion along with its control in the laboratory. For this study, groundwater was sampled from the underground pump station(i.e. I Gas Station) where corrosion was observed. Microbial diversity on the samples were then obtained by 16S rDNA methods. From this, microbial populations showing corrosion behaviors against metals were reported as Leptothrix sp.(Iron oxidizing) and Desulfovibrio sp.(Sulfur reducing) Iron oxidizing bacteria were dominantly participating in the corrosion of iron, while sulfate reducing bacteria were more preferably producing precipitate of iron. In case of galvanized steel and stainless steel, iron oxidizing bacteria not only enhanced the corrosion, but also generated its scale of precipitate. Sulfate reducing bacteria had zinc steel corroded greater extent than that of iron oxidizing bacteria. In the inactivation test, chlorine or UV exposure could efficiently control bacterial growth. However as the inactivation intensity being increased beyond a threshold level, corrosion rate was unlikely escalated due to augmented chemical effect. It is decided that microbial corrosion could be differently taken place depending upon type of microbes or materials, although they were highly correlated. It could be efficiently retarded by given disinfection practices.

A Fundamental Study for Design of Electric Energy Harvesting Device using PZT on the Road (도로용 압전발전체 시험모듈 설계를 위한 기초 실험 연구)

  • Lee, Jae-Jun;Ryu, Seung-Ki;Moon, Hak-Yong;Kwon, Soo-Ahn
    • International Journal of Highway Engineering
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    • v.13 no.4
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    • pp.159-166
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    • 2011
  • Green house gas emissions are increasing as development of the industrial economy of the international community. Many countries in the world are endeavoring to reduce green house gas emissions under severe climate change. In order to protect grobal warming, government is trying to reduce green gas emissions under "Low Carbon Green Growth Policy" and investing climiate-firendly industries such as renewable energy harvesting. Renewable energy has been rapidly developing as a result of investment for development technology of using natural energy such as solar, wind, tidal, etc. There are lots of waste energy in the road space. However, nobody is not interested in waste energy from the road space. This paper present a fundamentally experimental study of energy harvesting technique to use waste energy in the road. The waste energy in the road is covered a pressure and impact of vehicles on the road, the radiant heat from asphalt pavement, road noise and vibration etc. In this study, an energy harvesting device using piezoelectric element is proposed and various tests are conducted to investigate a characteristic of this device as function of impact loading based on piezoelectric effect behavior. This paper shows the energy harvesting results of the device using domestic piezoelectirc element as a function of impact load size and pavement types.

The Analysis about Factors That Influence the Motive of Partner Homicide (배우자살인의 동기에 영향을 미치는 요인분석)

  • Shin, Shik;Gong, Jung-Sick
    • Korean Security Journal
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    • no.12
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    • pp.201-224
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    • 2006
  • This study explained about the different motive of partner homicide and this homicide's elements which are feature of human sociology, home environment, school life and partner's trouble causes. After analyzed these element which affect to the motive of partner homicide, we couldn't find statistical differences of partner homicide motive by sex, having a job. However, there are small gap in an age group. Almost the homicide by expressive motive was a primary factor in the whole mass but, in the age of 40's, 11.1% is by the homicide of instrumental motive in the partner homicide. We need some more detail analysis and the partner murderer who growed up in very poor home circumstances commit Homicide by 100% expressive motive. This result suggested that murderer who was in the inferior home environment can do murder more easier than normal because of their emotional reason which are urgent, impulsive and contingent. On the other hand, their school life behavior during a growth process was useless for explain the motive of partner homicide. On one side, we could confirm that partner's trouble cause is a very useful element for explain the partner homicide, that is, if the if the level of conflict with partner's were high, the murder by expressive motive will grow up. But we must see the fact that the partner homicide's rate is 13.3% by instrumental motive in the low trouble causes very carefully for understand the partner homicide.

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The Study on the Effects of Parent Role Education Program for infants' health promotion - Focusing on the twelve-months results - (영아의 건강증진을 위한 부모역할교육 프로그램의 효과에 관한 연구 -생후 12개월의 결과를 중심으로-)

  • Han, Kyung-Ja;Kwon, Mi-Kyung;Bang, Kyung-Sook;Kim, Jung-Soo
    • Korean Parent-Child Health Journal
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    • v.5 no.2
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    • pp.129-144
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    • 2002
  • This longitudinal quasi-experimental research was conducted to develop parent role education program and to evaluate the effect of this program for mother-infant interaction, childrearing environment and infant development. The subjects were the healthy infants weighing over 2,500gm at birth, whose gestational age was more than 37weeks, and their healthy mothers. The sample consisted of eighteen mother-infant dyads for intervention group and sixteen dyads for control group. Data were collected from March 15th in 1999 to Jun 20th in 2000. The intervention group received programmed education consisted of discharge education, telephone counselling, and home visiting care. But control group were collected data without programmed education. In this study, the Nursing Child Assessment Teaching Scale(NCATS) devised by Barnard was used to determine the mother-infant interaction, HOME was used to determine the childrearing environment, and Griffiths mental development scale was used to determine the infant development. The data were analyzed using SPSS Win using chi-square test, t-test, and repeated measure ANOVA. This study was focused on the results of twelve months time point. Summaries of the results were as follows: 1. There was no significant difference in mother-infant interaction(NCATS) between intervention group and control group. But both of two groups showed significantly higher in interaction score at twelve months than at six months in the subscales of social-emotional growth fostering, and responsiveness to caregiver. 2. There was no significant difference in childrearing environment(HOME) between two groups at twelve months. But when each subscale of HOME was examined, intervention group showed higher scores in the dimensions of maternal involvement with child(p=.001), and maternal emotional-verbal responsivity(p=.048). 3. There was no significant difference in GQ of the Griffiths mental development scale between two groups, although significant difference was found in performance subscale. 4. Infant development at twelve months showed significant correlation with mother-infant interaction and childrearing environment at six months, although mother-infant interaction and childrearing environment at twelve months did not show significant correlations with infant development at twelve months. 5. Developmental scores at six months showed significant correlations with variety in daily stimulation, and mother's emotional, verbal responsivity, whereas developmental scores at twelve months showed significant correlations with acceptance of child behavior at six months, and appropriate play material at twelve months. In conclusion, the maternal education program for primipara showed long term effect in some categories in organizing the childrearing environment, and fostering the infant development. We suggest further study and implications of parent role education program for high risk parents such as parents in low economic status or with premature babies.

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Properties of Au Clusters Supported on $TiO_2$ Studied by XPS, ISS, AES, and TPD (XPS, ISS, AES, TPD를 이용한 $TiO_2$ 위에 지지된 Au 클러스터의 특성 연구)

  • Kim, Dae Young
    • Journal of the Korean Chemical Society
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    • v.42 no.6
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    • pp.607-617
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    • 1998
  • Au was dosed on $TiO_2(001)$ film grown epitaxially on Mo(100) surface in about 90 ${\AA}$ thickness. The growth mode of Au, thermal behavior and stability of the Au clusters, and the binding energy shift of Au 4f with the change in the amount of Au loading were studied by Auger Electron Spectroscopy (AES), Temperature Programmed Desorption (TPD) spectroscopy, Ion Scattering Spectroscopy (ISS), and X-ray Photoelectron Spectroscopy (XPS). Au grows three dimensionally on $TiO_2(001)$ film and the average size of Au clusters prepared at low temperature is smaller than those at higher temperature and the size increases with temperature irreversibly. Au clusters on $TiO_2(001)/Mo(100)$ start evaporation at 1000 K. TPD spectra of Au show very asymmetric peaks with the same leading edges irrespective of the amount of Au loading. The temperature at the peak maximum increases with the amount of Au. The desorption energy of Au obtained from the leading edge analysis of the TPD spectra is about 50 kcal/mol. The initial sticking coefficient of Au on $TiO_2(001)$ is constant in the temperature range of 200-600 K. The binding energy of Au 4f for the Au loaded on the film less than 2.0 MLE shifts to higher energy compared with the bulk Au. The shift is +0.3 eV at 0.1 MLE Au amount.

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Coupled Finite Element Analysis of Partially Saturated Soil Slope Stability (유한요소 연계해석을 이용한 불포화 토사사면 안전성 평가)

  • Kim, Jae-Hong;Lim, Jae-Seong;Park, Seong-Wan
    • Journal of the Korean Geotechnical Society
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    • v.30 no.4
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    • pp.35-45
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    • 2014
  • Limit equilibrium methods of slope stability analysis have been widely adopted mainly due to their simplicity and applicability. However, the conventional methods may not give reliable and convincing results for various geological conditions such as nonhomogeneous and anisotropic soils. Also, they do not take into account soil slope history nor the initial state of stress, for example excavation or fill placement. In contrast to the limit equilibrium analysis, the analysis of deformation and stress distribution by finite element method can deal with the complex loading sequence and the growth of inelastic zone with time. This paper proposes a technique to determine the critical slip surface as well as to calculate the factor of safety for shallow failure on partially saturated soil slope. Based on the effective stress field in finite element analysis, all stresses are estimated at each Gaussian point of elements. The search strategy for a noncircular critical slip surface along weak points is appropriate for rainfall-induced shallow slope failure. The change of unit weight by seepage force has an effect on the horizontal and vertical displacements on the soil slope. The Drucker-Prager failure criterion was adopted for stress-strain relation to calculate coupling hydraulic and mechanical behavior of the partially saturated soil slope.

IDIOPATHIC GINGIVAL FIBROMATOSIS AT BIRTH (출생 시 발생한 특발성 치은섬유종증)

  • Lee, Hyo-Seol;Choi, Hyung-Jun;Choi, Byung-Jai;Sohn, Hyung-Kyu;Lee, Jae-Ho
    • Journal of the korean academy of Pediatric Dentistry
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    • v.35 no.4
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    • pp.766-770
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    • 2008
  • Gingival fibromatosis is a rare condition characterized by varying degrees of gingival hyperplasia. Gingival fibromatosis usually occurs as an isolated disorder or can be associated with a variety of other syndromes. It usually appears at the time of eruption of permanent dentition but, can develop at the time of eruption of the primary dentition and rarely at birth. It may deform palatal contour and subsequently restrict the tongue movement, resulting in interference during speech and mastication. In addition, it incapacitates maintenance of normal lip closure. A 14-month-old girl visited the department of pediatric dentistry, Yonsei University Dental Hospital, for the congenital gingival overgrowth. There was no one in the family, who showed similar pattern of gingival growth. The intraoral clinical examination revealed generalized severe gingival enlargement throughout the maxillary and the mandibular arches. Enlarged gingival tissue was pink and had firm consistency. She was referred for chromosomal analysis, which confirmed absence of any known syndrome. Under local anesthesia, "Punch-biopsy" was performed on the labial area, and the specimen was histologically diagnosed as gingival fibromatosis. For she did not have any medical problem nor familiar history, she was diagnosed as having idiopathic gingival fibromatosis. Regarding her age and behavior, close follow-up was decided.

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Case Studies for Nanomaterials' Exposure to Environmental Media (나노물질의 환경 매질별 노출 사례 조사)

  • Umh, Ha Nee;Roh, Jinkyu;Lee, Byoung-Cheun;Park, Sumin;Yi, Jongheop;Kim, Younghun
    • Korean Chemical Engineering Research
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    • v.50 no.6
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    • pp.1056-1063
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    • 2012
  • Recent rapidly growth in nanotechnolgies is promised novel benefits through the exploitation of their unique industrial and biomedical applications. With increasing utilization of nanomaterials in consumer products, the potential release of nanomaterials into the environment and their impacts on the ecosystem and human health have been the issues of concern. Nanomaterials that was exposed unintentionally in environment might be accumulated in various environmental media, and finally it will be influenced to human and ecosystems. Therefore, it is important to understand the fate and behavior of nanoparticles for understanding effects on environmental media (air, water, and soil phase). Therefore, in this work, we investigated the several cases for environmental exposure of nanomaterials and suggested the direction of further research. In workplace, exposure to air media is dominant, but finally waste and wastewater was moved to the water and soil phase. In addition, we found the existing sewage treatment plant was not suitable to remove completely nanomaterials in wastewater flow. To deeper study, environmental monitoring tool must be developed additionally and we suggested the several analyzing method for aged and pristine physicochemical properties of nanomaterials exposed into environmental media. This review for nanomaterials' exposure to environmental media will be helpful to investigate the environmental fate of nanomaterials and define the suitable treatment method for nano-waste.

The Development and the Effects of Group Counseling Program Based on Reality Therapy for the Decrease of Elementary School Students' Academic stress (초등학생의 학업스트레스 감소를 위한 현실요법 집단상담 프로그램의 개발 및 그 효과)

  • Woo, Hyun-Ju
    • 한국초등상담교육학회:학술대회논문집
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    • 2004.01a
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    • pp.137-168
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    • 2004
  • The purpose of this study is to find the effect of group counseling program based on reality therapy that is developed by researcher and to investigate whether the program can decrease the academic stress of elementary school students. For this study, firstly a framework for academic stress decrease programs is established and a program is developed in accordance with the framework. Secondly, in order to verify the effect of the program, both quantitative and qualitative methods were applied to 6th grade students through a academic stress test. Experimental group and control group is composed of 18 students, each. During a two month-experimental period, group counseling program based on reality therapy was implemented to the experimental group for 10 sessions but not to the control group. The program included nicknaming, recreation activities to promote friendliness among participants and other various activities to decrease the academic stress. The results of the quantitative and statistical research are as follows: First, group counseling program based on reality therapy is effective to decrease of elementary school students' academic stress. Second, sub factor(such as perceptions of academic stress cause, symptoms of academic stress)of academic stress is significantly decreased in experimental group. But no significant difference is found in control group. Third, for some sub factor(career, friend-relationship, house-environment, behavior-symptom), an independent-sample test and a paired-sample test showed different results: the paired-sample test showed no significant differences in experimental group while the independent-sample test showed significant decrease of academic stress. The results of the qualitative research based on student commentaries on the program is as follows: First, experimental group students report that the program based on reality therapy was effective to decrease of academic stress. Second, experimental group students' commentaries shows that students could remember the WDEP, 'act' factor and acquire the solution of academic stress. Third, for a group counseling, students reported the interest about positive feedback, searching on themselves, growth of human-relationship. This means that the program developed on this study is suitable for group counseling program. In conclusion, this study proves that group counseling program based on reality therapy is effective to decrease of elementary school students' academic stress by increasing individual internal-control. Therefore, it is necessary that we should develop and apply similar program to elementary school students in other to decrease students' academic stress. school students in other to decrease students' academic stress.

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