• KSBB Journal
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• 제21권5호
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• pp.376-383
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• 2006

솔잎착즙액(PNE)은 엽록소 함유량(40.8 mg/100 g)이 높고 비타민 C도 다량함유(149 mg/100 g)하고 있어 기능성 식품으로 개발가능성이 높으며, 솔잎 발효액은 향미가 좋아지고 기능성이 높아져서 고부가가치가 크므로 발효의 조건과 활성도를 조사 분석하여 고기능성 솔잎발효액(SFPE)을 개발하고자 하였다. 솔잎자체발효(SFPE)에 관여하는 균을 발효단계에 따라 분리, 선발하고 동정하였다. GPYA배지에서 발효균을 스크린하여 Pichia galeiformis, Candida boidinii, Pichia species, Candida species1, Candida species2, Candida ooidensis, Saccharomyces cerevisiae, Candida karawaiewii를 선발하였으며, 발효가 진행됨에 따라 2년차에는 Candida boidinii, Candida species2, Candida ooidensis, Saccharomyces cerevisiae가 선발되었고, 3년차에는 Candida species1&2가 선발되어 Candida species2가 우점종으로 나타났다. 그러나 발효 4년차에는 어떤 균도 선발되지 않았다. Candida species2는 Candida ernobii와 99.65%의 homology를 보였다. E. coli, Bacillus subtilis와 Staphylococcus aureus에 대해서 SFPE를 농도별로 처리하였을 때 높은 항균활성을 확인하였다. SFPE 1, 4, 7의 항산화 활성은 $0.025{\mu}{\ell}/ml$의 농도로 처리하였을 경우 SFPE 1, 4, 7이 약 34.8%의 NBT scavenging의 활성을 보여주었고, $0.2{\mu}{\ell}/ml{\sim}0.3{\mu}{\ell}/ml$의 농도에서는 SFPE 1, 4, 7이 약 90%에 달하는 NBT scavenging 의 활성능력을 확인할 수 있었다. 또한 전기생리학적 실험을 통하여 솔잎발효액이 쥐의 혈관수축 이완에 미치는 효과를 분석한 결과 Phenylephrine(PE)($10^6M$)으로 유발된 수축반응에 대해 SFPE를 0.15 mg/ml과 0.3 mg/ml을 첨가하였을 때 혈관이 다시 이완되었으며, ATP 민감성 $K^+$ 채널 억제제인 glibenclamide($10^5M$)을 처리하였을 때 SFPE 7 효과가 나타나지 않은 것으로 보아 SFPE의 혈관 이완작용이 세포막 ATP-민감성 $K^+$ 통로를 활성화시켜 이루어짐을 확인하였다.

• 한국식생활문화학회지
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• 제19권3호
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• pp.273-284
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• 2004

This study is on the changing pattern of fish-and-shellfish uses during the last two thirds of Chosun period on the premise that they are influenced by development of fishing technology. With a literary approach, this paper researches how fish-and-shellfish production and consumption pattern changed, especially in relation to technological development of fisheries. First, we examine into the changes of fishery production. And next, we analyze the kind and cooking method of fish-and-shellfish in [Eum-sik-di-mi-bang](1670) and [Gyu-hap-chong-seo](1815). The analysis of two books revealed that there were significant differences in fish-and-shellfish uses. Because the two books were written with a gap of 145 years and the development of fishing technology and remarkable changes of fishery production affected on the fish-and-shellfish uses. Due to primitive fishing tools and skills, fresh-water and reverse-river-fish and shellfish had been caught in substantial amount until the middle ages of Chosun period. As a result, the availability of seafood were limited extremely even in the upper classes. These situations are evidenced by the analysis of [Eum-sik-di-mi-bang]. Only 12 kinds of fish-and-shellfish are described in [Eum-sik-di-mi-bang]. Most of the sesfoods is mollusc which is easy to catch. As for the salt-water fish, dried cod and dried herring were mentioned. Mullet, the reverse-water-fish, is used most frequently. Only one kind of 'Hoe', which needs extreme freshness, is described. This means that the use of fresh fish-and-shellfish was very limited to some kinds which could be caught near the village. As the netting fishery began to be developed in the 18th century, the production of some salt-water fishes, such as anchovy, shrimp, yellow corvina, pollack, and herring, had increased remarkably to make marine resources more available. Small fish, such as anchovy and shrimp were preserved as 'Jeot-gal' and sold nationwide. Therefore, 'Jeot-gal' and seafood could be used in Kimchi around this time and had a deep influence on the change of Kimchi in taste and nutrition. In [Gyu-hap-chong-seo], 33 kinds of fish-and-shellfish are described. Including cod and herring, 17 kinds of sea water fish and mollusc are mentioned. Some of these are consumed in fresh state, neither as dried nor as salted. Because the merchants promoted the transport of seafoods to other regions according to the growth of commercial economy. As a result the diet of the people could be enriched by the various seafoods.

• 한국환경농학회지
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• 제20권4호
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• pp.211-217
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• 2001

본 연구는 경작지 중금속 오염지역의 식물복원을 위한 방안으로, Zn, Cu처리가 담배식물의 생리반응에 영향하는 형태적인 변화와 엽령에 따른 중금속 축적 양상 및 무기이온의 흡수관계를 분석한 결과는 다음과 같다. Zn, Cu에 따른 간장의 생장감소는 1.0 mM이하에서는 작았고, 지상부 건물중보다는 지하부의 건물중을 크게 감소시켰다. 특히 지하부의 건물중은 약 50%가 감소하였다. Zn, Cu처리시 지상 ${\cdot}$ 지하부의 축적은 처리농도가 증가함에 따라 완만히 증가하다가 2.0 mM이상에서는 급격히 증가하였다. 엽령에 따라 Zn은 노화엽에서, Cu는 생장엽에서 축적량이 많았다. 주당 축적량은 2.0 mM에서 Zn은 146,000 mg/kg, Cu는 20,500 mg/kg이었고, 식물수확시기는 Zn은 영양생장기에 Cu는 생식생장기가 적합하였다. Zn, Cu처리에 따른 Mg, Mn, Fe함량은 처리농도가 증가할수록 감소하였다. 지상 ${\cdot}$ 지하부 모두 Zn처리구에서는 Cu함량은 감소하고, Cu처리구에서는 Zn함량은 증가하였다.

• 자원환경지질
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• 제45권5호
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• pp.535-549
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• 2012

이 연구의 주목적은 영광-나주지역에 분포하는 송림-대보화강암류를 지질시대, 광물조성, 화학성분과 조직에 따라 각섬석-흑운모화강섬록암, 흑운모화강암, 반상화강암과 복운모화강암의 네가지 암상으로 구분하였다. 이들 화강암류는 동원마그마로부터 분화된 일련의 분화산물로 이루어진 화성암 복합체이며, 페름기말에서 트라이아스기 사이에 옥천습곡대와 영남육괴 사이에서 지괴들의 충돌운동으로 유발된 응력장하에서 형성되었고, 그리고 이 변형대의 형성기에 전후하여 정치된 화강암류가 대보운동을 받아 엽리상화강암류와 비변형화강암류를 형성하였다. 이들 영광-나주지역에 분포하는 화강암질암류의 구성광물 성분은 사장석, 흑운모. 백운모와 각섬석으로 구성되어있다. 사장석은 오리고클레스($An_{19.3-27.7}$)와 안데신($An_{28.4-31}$)성분에 해당하며 누대구조의 성분변화도 좁은영역에 치우치면 이는 느린 결정작용과 이들 결정들의 성장이 최종고결작용 이전에 일어났음을 보여준다. 흑운모의 Mg 함량은 산소의 압력과 분화도의 증가에 따른다(프로고파이트에서 시데로피라이트로 변함). $Al^{iv}$/$Al^{total}$ 관계는 전체 알루미나양에 따라 증가한다. 백운모는 일차기원이며 높은 $TiO_2$함량을 보이는 세라도나이트이다. 각섬석은 회각섬석류에 속하며 지질압력계로 계산한 화강암질암의 고결심도는 11.3-17.2 Km이다.

• 생물환경조절학회지
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• 제11권1호
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• pp.12-17
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• 2002

수출용 토마토 하계육묘시 육묘 용기 와 육묘 일수를 달리 함으로써 나타나는 묘소질의 차이를 구명하고, 묘소질이 다른 묘를 정식하여 재배할 경우 생육과 수량에 미치는 영향을 구명코자 하였다. 육묘시의 묘 생육은 폿트 묘가 플러그 묘보다 좋았으며, 폿트 묘는 육묘 일수가 길수록 좋았으나 플러그 묘는 육묘 일수에 따른 차이가 없었다. 정식 60일 후의 생육도 정식 직전과 같은 경향이었다. 1화방 수확 개시기는 폿트 묘가 플러그 묘보다 빨랐으며, 육묘 일수가 길수록 빨랐다. 4개월간 수량은 폿트 묘가 플러그 묘 보다 유의하게 많았는데 수확 2개월까지 초기수량이 현저히 많았다. 폿트 묘는 수확 2개월까지는 45일 묘와 35일 묘는 큰 차이가 없었고 25일 묘가 가장 적었으나 수확 3개월째부터는 육묘일수에 따른 차이가 없었다. 플러그 묘에서는 수확 3개월가지는 35일 묘가 가장 많았고 25일 묘, 45일 묘 순이었으나 수확 4개월째에는 육묘 일수에 따른 차이가 없었다. 따라서 수출용 모모타로 요쿠 재배시, 수출시기와 수출기간에 따라 육묘 용기 용량 및 육묘 일수를 결정하는 것이 좋을 것으로 생각되었다.

• Journal of Nutrition and Health
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• 제34권1호
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• pp.30-38
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• 2001

This research was conduced to 1) examine the influence of milk group intake on the dietary, considering dietary diversity and nutrient adequacy, 2) identify which foods truly contribute to the intake of specific nutrients 3) point out the dietary problems of this age group. The subjects were 664(male 357, female 307) healthy toddlers of the ages of 12-36 months, who had neither been taking any medicine-type supplements nor had any diseases that affect the dietary intakes, chosen among the outpatients of university and private hospitals in Seoul and Kyonggi province during Mach-August, 1999. The 24 hour recall method and CAN Pro(food analysis program) were used to analyze the amount of nutrient intake. The grouping of the subjects for the analysis by monthly age was based on the 98 Korean Child growth standard. The results that the milk group intake showed significant negative correlations with the grain group(r=-0.194, p<0.01), the vegetable group(r=-0.115, p<0.01) and the fruit group(r=-0.125, p<0.01). It also showed that the more the milk group intake, the lower the Dietary Diversity Score(r=-0.131, p<0.01), and the more the intake of raw milk, the more dificient the intake of iron(r=-0.211, p<0.01), vitamin A(r=-0.169,p<0.01), vitamin B$_1$(r=-0.078, p<0.05) and vitamin C(r=-0.187, p<0.01). Foods contributory to nutrient intake for of 12-14 months of were mostly infant formula, and the contribution ratio was high. In the age of 15-17 months, the subjects obtained most of the nutrients from raw milk., liquid-type yoghurt, soybean milk, cooked rice, etc. As for iron intake, the age of 21-23 month obtained the most from ionic drinks, and 24-29 months from raw milk, chocolate cakes, ionic drinks, etc. Though the NAR(Nutrient Adequacy Ratio) and MAR(Mean Adequacy Ratio) generally tended to increase accordingly with the DDs, as for nutrients Ca, vitamin A, vitamin B$_1$, and vitamin C the NAR marked the highest when the DDS marked the lowest(due to infant formula). And for iron, even when the DDS was 5 the NAR value was low. Im conclusion, the more the milk group intake, the lower was the dietary diversity. High dependency on infant formula and others from milk group over the age of 12 months proved to result in a decrease of nutritious solid food intake, iron deficiency and a low dietary diversity score, which can lead to an unbalanced diet. Therefore, nutrition education towards mothers/caretakers of toddler is of urgent need, in order to reduce the fluid milk group intake of low nutritional density and to provide various solid foods the children. (Korean J Nutrition 34(1):30-38, 2001)

• Asian-Australasian Journal of Animal Sciences
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• 제21권10호
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• pp.1395-1403
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• 2008

The objective of the present study was to estimate genetic parameters for economic traits in Markhoz goats. Data collected from 1993 to 2006 by the Markhoz goat Performance Testing Station in Sanandaj, Iran, were analyzed. The traits recorded as body weight performance at birth (BW), weaning (WW), six month (6MW), nine month (9MW), yearling (YW) and yearling fleece weight (YFW) were investigated. Least square analyses were used for estimation of environmental effects. Genetic parameters were estimated with single and multi trait analysis using restricted maximum likelihood (REML) procedures, under animal models. By ignoring or including maternal additive genetic effects and maternal permanent environmental effects, five different models were fitted for each trait. The effects of sex, type of birth, age of dam and year of birth on the all body weights were significant (p<0.01), but had no effects on YFW except year of birth. Age of kids had significant influences on WW and 6MW (p<0.01). A log likelihood ratio test was carried out for choosing the most suitable model for each trait. Total heritability estimates for YFW and growth traits varied from 0.16 for YFW and WW to 0.41 for YW. For all traits, maternal heritability was lower than direct heritability, ranging from 0.06 for BW to 0.01 for 6MW and 9MW. The magnitude of $c^2$ was more substantial for BW than the others, and relative importance was reduced from 0.12 for BW to 0.04 for 9MW. The direct additive genetic correlations estimates were positive and varied from 0.21 between BW-YW to 0.96 between WW-6MW. Direct additive genetic correlations between YFW and body weight traits were positive and ranged from 0.14 between BW-YFW to 0.67 between 6MW-YFW. For all traits, the corresponding estimates for phenotypic correlation were positive and lower than genetic correlations. The maternal additive genetic correlations between various traits were varied and ranged from -0.19 between 9MW-YFW to 0.96 between 6MW-9MW. The estimates of the maternal permanent environmental correlations between various traits were positive and ranged from 0.33 between WW-YFW to 0.93 between WW-6MW. Also, the environmental correlations between various traits ranged from 0.01 between BW-YFW and WW-YFW to 0.70 between 9MW-YW. Estimates of genetic parameters for various traits in this study confirm that selection should be applied on WW for genetic improvement in Markhoz goats.

• Journal of Microbiology and Biotechnology
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• 제27권8호
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• pp.1428-1440
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• 2017

Phomopsis sp. XP-8 (an endophytic fungus) was previously found to produce pinoresinol diglucoside (PDG), a major antihypertensive compound of Tu-Chung (the bark of Eucommia ulmoides Oliv.), which is widely used in Chinese traditional medicines. In the present study, two bioconversion systems were developed for the production of PDG in Tris-HCl buffer containing glucose and Phomopsis sp. XP-8 cells (both resting and freeze-dried). When other factors remained unchanged, the bioconversion time, glucose concentration, cell ages, cell dosage, pH, temperature, and stirring speed influenced PDG production in a similar and decreasing manner after an initial increase with increasing levels for each factor. Considering the simultaneous change of various factors, the optimal conditions for PDG production were established as 70 g/l cells (8-day-old), 14 g/l glucose, $28^{\circ}C$, pH 7.5, and 180 rpm for systems employing resting cells, and 3.87 g/l cells, 14.67 g/l glucose, $28^{\circ}C$, pH 7.5, and 180 rpm for systems employing freeze-dried cells. The systems employing freeze-dried cells showed lower peak PDG production ($110.28{\mu}g/l$), but at a much shorter time (12.65 h) compared with resting cells (23.62 mg/l, 91.5 h). The specific PDG production levels were 1.92 and $24{\mu}g$ per gram cells per gram glucose for freeze-dried cells and resting cells, respectively. Both systems indicated a new and potentially efficient way to produce PDG independent of microbial cell growth.

• 한국과학교육학회지
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• 제9권1호
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• pp.19-37
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• 1989

The purpose of this study was to investigate the development of logical thinking and scientific reasoning pattern of Korean high school students. To carry out this study subjects were selected about 2,000 Junior high school students, and about 4,100 senior high school students throughout the nation. They were identified as concrete, transitional or formal operational stage with the use of TOLT(the Test of Logical Thinking) by Tobin and Capie(1980), and TOSR(the Test of Scientific Reasoning) by W.A Farmer(1986). This study turned out that more than 76% of Junior high school students were classified as the concrete operational stage and about 44% of senior high school students were classified as the formal operational stage, while about 26% of them were still in the concrete operational level. This study showed that the main factor of the intellectual development of students is learning by the gradual advancement of their grades and especially entrance into the senior high school rather than by the physical growth. This study also showed that there are the take-off stage of the development of logical thinking between fourteen and fifteen years of their ages. Less than 25% of junior high school students were in the formal operational stages which are capable of control of variables, probabilistic, correlation and combinational logic in problem-solving situation, while 33-54% of senior high school students were in the formal operational levels. 38% of junior high school students were in the formal operational stage which is capable of proportional logic, while about 55% of senior high school students were in the formal operational stage. Less than 20% of senior high school students were classified as group of highly capable of scientific reasoning, while more than 23% of them were classified as group of poor capability. It also turned out that there are differences or no differences between male and female students of each school in problem-solving situation regarding each logic approach. These differences were proved to be fluctuating depending on the situations and their grades. The other results of this study is similar to those of other researches such as Tomlinson-Keasey 1972, Coleman 1973, Lawson 1973, Lawson and Renner 1974, Neimark 1975, Han 1982, and Kim 1989.

• 대한소아치과학회지
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• 제47권1호
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• pp.17-24
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• 2020

이 연구의 목적은 소아 얼굴 연조직의 선형 길이를 비교하여 삼차원 입체영상의 정확성과 재현성을 평가하는 것이다. 연구는 7 - 12세 아동 15명을 대상으로 하였다. 환자의 안모에 23개의 계측 점을 표시하고, 전자 캘리퍼를 이용하여 16 개의 선형길이를 직접 2번 계측하였다. 3차원(3D) 스캐너를 이용하여 2개의3D 얼굴 영상을 만들었으며, 직접계측과 동일한 16개의 선형 길이를 계측하였다. 연구결과 정확성 평가에서 전체 평균 차이 값은 0.9 mm였다. 3D 영상의 재현성은 직접계측의 재현성과 거의 유사한 정도로 나타났다. 이에 따라 이 3D 영상장비는 소아에서 임상적으로 충분한 정확성과 재현성을 가지고 있었다. 3D 영상 시스템은 정확한 이미지를 위하여 대상의 협조가 필요하다. 소아의 정확한 3D영상을 얻기 위한 구체적인 지시사항을 바탕으로 촬영을 한다면, 이 3D 영상 장비는 향후 소아의 성장과 발달을 조사하고, 소아교정치료에서 결과를 평가하는데 유용하게 사용될 수 있을 것이다.