• Title/Summary/Keyword: growing medium

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Screening of Fungal Strains Producing Lovastatin, an Antihypercholesterolemic Agent (콜레스테롤합성저해제 lovastatin을 생산하는 곰팡이 균주의 탐색)

  • Bang, In-Young;Whang, Seung-Whan;Kim, Jung-Wan;Kim, Sang-Yong;Park, Cheon-Seok
    • Korean Journal of Food Science and Technology
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    • v.35 no.3
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    • pp.442-446
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    • 2003
  • Over two hundred fungal strains from Korean soil were tested for the production of cholesterol-lowering agent, lovastatin. Each fungal strain was cultivated in the rapeseedmeal production medium (RPM). After growing for 7 days, the presence of lovastatin in the culture was examined by TLC analysis and HPLC. Nine different fungal strains were determined to produce detectable amounts of lovastatin, among which one fungal strain isolated from barnyard manure of Kanghwa island produced 25.58 mg/L of lovastatin in the production medium. The morphological observation combined with the determination of 18S-rRNA sequence suggested that the selected strain belongs to a member of Aspergillus sp..

Micro-Chemical Structure of Polyaniline Synthesized by Self-Stabilized Dispersion Polymerization

  • NamGoong, Hyun;Woo, Dong-Jin;Lee, Suck-Hyun
    • Macromolecular Research
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    • v.15 no.7
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    • pp.633-639
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    • 2007
  • A variety of NMR techniques were applied to the micro-chemical structural characterization of polyanilines prepared via an efficient synthetic method in a self-stabilized dispersion medium in which the polymerization was conducted in a heterogeneous organic/aqueous biphasic system without any stabilizers. Here, the monomer and growing polymer chain were shown to function simultaneously as a stabilizer, imparting compatibility for the dispersion of the organic phase, and as a form of flexible template in an aqueous reaction medium. Polymerizations predicated on this concept generated polyanilines with a low defect content: solution state $^{13}C-NMR$ and solid $^{13}CDD/CP/MAS$ spectroscopy indicated that the synthesized HCPANi and its soluble derivative, HCPANi-t-BOC, evidenced distinctly different NMR spectra with fewer side peaks, as compared to conventionally prepared PANis, and the complete structural assignments of the observed NMR peaks could be determined via the combination of both 1D and 2D techniques. Ortho-linked defects in HCPANi were estimated to be as low as 7%, as shown by a comparison of the integration of the carbonyl carbon resonance peaks.

An Empirical Study on ERP Package in Korean SMEs (한국 중소기업 기업관리 패키지에 관한 실증적 연구)

  • Kim, Seung-Gweon;Kim, Jae-Young;Park, Seung-Bong
    • Journal of the Korea Society of Computer and Information
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    • v.12 no.1 s.45
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    • pp.243-252
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    • 2007
  • In recent years, there has been a growing concern about enterprise resource planning (ERP) implementation in small and medium-sized enterprises (SMEs). In this study, we investigate the relationship among ERP software qualify, vendor support, user information satisfaction. and performance of ERP implementation. The empirical findings of this paper will allow SMEs to identify the importance of relationships with vendors and software itself for the successful implementation of ERP systems. The results provide a useful basis for the SMEs which have limited information capability to focus their attention on the software quality and vendor support in overcoming difficulties and obstacles facing the ERP implementation.

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Enhanced Antibiotic Production by Streptomyces sindenensis Using Artificial Neural Networks Coupled with Genetic Algorithm and Nelder-Mead Downhill Simplex

  • Tripathi, C.K.M.;Khan, Mahvish;Praveen, Vandana;Khan, Saif;Srivastava, Akanksha
    • Journal of Microbiology and Biotechnology
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    • v.22 no.7
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    • pp.939-946
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    • 2012
  • Antibiotic production with Streptomyces sindenensis MTCC 8122 was optimized under submerged fermentation conditions by artificial neural network (ANN) coupled with genetic algorithm (GA) and Nelder-Mead downhill simplex (NMDS). Feed forward back-propagation ANN was trained to establish the mathematical relationship among the medium components and length of incubation period for achieving maximum antibiotic yield. The optimization strategy involved growing the culture with varying concentrations of various medium components for different incubation periods. Under non-optimized condition, antibiotic production was found to be $95{\mu}g/ml$, which nearly doubled ($176{\mu}g/ml$) with the ANN-GA optimization. ANN-NMDS optimization was found to be more efficacious, and maximum antibiotic production ($197{\mu}g/ml$) was obtained by cultivating the cells with (g/l) fructose 2.7602, $MgSO_4$ 1.2369, $(NH_4)_2PO_4$ 0.2742, DL-threonine 3.069%, and soyabean meal 1.952%, for 9.8531 days of incubation, which was roughly 12% higher than the yield obtained by ANN coupled with GA under the same conditions.

Optimal Medium Conditions for the Detection of Cellulolytic Activity in Ganoderma lucidum

  • Jo, Woo-Sik;Bae, Soon-Hwa;Cho, Doo-Hyun;Park, So-Deuk;Yoo, Young-Bok;Park, Seung-Chun
    • Mycobiology
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    • v.37 no.4
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    • pp.313-316
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    • 2009
  • To determine the optimal medium conditions for the detection of the cellulolytic activity in Ganoderma lucidum, we varied three media conditions: dye reagent, pH, and temperature. First, we evaluated the use of four dyes, Congo Red, Phenol Red, Remazol Brilliant Blue, and Trypan Blue. To observe the effect of pH on the chromogenic reaction, we also made and tested various media spanning acidic and alkaline pHs, ranging from 4.5 to 8.0. Furthermore, in order to research the effect of temperature on the clear zone and the fungus growing zone, we tested temperatures ranging from 15 to $35{^{\circ}C}$. On the whole, the best protocol called for Ganoderma lucidum transfer onto media containing Congo red with pH adjusted to 7.0, followed by incubation at $25{^{\circ}C}$ for 5 days. Our results will be useful to researchers who aim to study extracellular enzyme activity in Ganoderma lucidum.

Application of Subirrigation Using Capillary Wick System to Pot Production

  • Lee, Chi-Won;So, In-Sup;Jeong, Sung-Woo;Huh, Moo-Ryong
    • Journal of agriculture & life science
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    • v.44 no.3
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    • pp.7-14
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    • 2010
  • Alternative subirrigation way, capillary wick system (CWS) was tested to reduce labor cost, waste water, contamination of ground water, and use of fungicide compared to overhead irrigation system (OIS). CWS helped reduce remarkably the working hours for watering from 4 hours in OSI to just 5 minutes. Labor cost was saved 98% in CWS compared to OIS. By the physical characteristics of various growing media, 1 coconut coir+2 perlite (v/v) mixture was selected because it had an ideal distribution of three phase, e.g. 1 solid: 1 liquid: 2 gas phase. Medium mixture containing scoria had so high bulk and particle density to hurt root. In bark-containing medium, the liquid phase and the percent saturation of liquid phase with time elapsed was lower than that of other mixture. It meant that the mixture contained very low level of water. Application of CWS for cyclamen pot production played an important role in reducing the incident of fusarium wilt symptom from 18% in conventional over watering system to 4%. Cyclamen pot irrigated by capillary wick had shorter petiole and more leaves than those by overhead watering. As a result, this system was highly beneficial to get uniform pot products with high quality. It improved water and nutrient solution efficiency relative to conventional overhead irrigation system (OIS).

AM600: A New Look at the Nuclear Steam Cycle

  • Field, Robert M.
    • Nuclear Engineering and Technology
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    • v.49 no.3
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    • pp.621-631
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    • 2017
  • Many developing countries considering the introduction of nuclear power find that large-scale reactor plants in the range of 1,000 MWe to 1,600 MWe are not grid appropriate for their current circumstance. By contrast, small modular reactors are generally too small to make significant contributions toward rapidly growing electricity demand and to date have not been demonstrated. This paper proposes a radically simplified re-design for the nuclear steam cycle for a medium-sized reactor plant in the range of 600 MWe. Historically, balance of plant designs for units of this size have emphasized reliability and efficiency. It will be demonstrated here that advances over the past 50 years in component design, materials, and fabrication techniques allow both of these goals to be met with a less complex design. A disciplined approach to reduce component count will result in substantial benefits in the life cycle cost of the units. Specifically, fabrication, transportation, construction, operations, and maintenance costs and expenses can all see significant reductions. In addition, the design described here can also be expected to significantly reduce both construction duration and operational requirements for maintenance and inspections.

The Implementation of Smart Factories: Empirical Evidence from Korean Small and Medium-Sized Enterprises (스마트팩토리 도입 영향요인에 관한 실증연구: 우리나라 중소제조기업을 중심으로)

  • Chung, Jiyoon
    • Asia-Pacific Journal of Business
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    • v.13 no.2
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    • pp.79-94
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    • 2022
  • Purpose - The purpose of this study is to examine firm-level attributes related to Korean manufacturing small and medium-sized enterprises' (SMEs') decisions to implement smart factories. Design/methodology/approach - This study uses the provided by the Ministry of SMEs and Startups of Korea and the Korea Federation of SMEs. Manufacturing SMEs' decisions to implement smart factories in 2018-2019 were analyzed using multinomial logit and ordered logit models. Findings - The findings of this study suggest that firms' decisions to implement smart factories were positively related to firm size, R&D intensity, international market scope, and transactional relationships with customers. However, smart factory implementation decisions were not related to firm age and CEO gender. Research implications or Originality - This study illuminates firm-level attributes that may drive organizational innovation in the era of Industry 4.0 and thus contributes to the innovation adoption literature. This study also contributes to growing research on smart factories by analyzing the actual, progressive decisions to implement smart factories, as opposed to perceived intentions to implement them.

Introduction of a speedy growing trait into Pleurotus eryngii by backcrossing (큰느타리(새송이)버섯에서 역교배를 통한 속성형질의 도입)

  • Im, Chak-Han;Kim, Min-Keun;Je, Hee Jung;Kim, Kyung-Hee;Ryu, Jae-San
    • Journal of Mushroom
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    • v.10 no.2
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    • pp.49-56
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    • 2012
  • Two strains which have good traits in quality and speedy growing were selected based on previous report(Ryu, 2007) to breed a new strain carrying the traits in it. KNR2322 carrying high quality and KNR2503 carrying a speedy growing trait were cultivated to harvest monokaryons. The monokaryotic mycelia were characterized in growth rate, color, and stability. Selfing of monokaryons from KNR2322 and crossing between KNR2322- and KNR2503-derived monokaryons were performed. Two parental lines, $KNR2322-4{\times}37-6{\times}12(B)$ showing thremmatological values in weight and quality, 68.5g and 6.5 and $KNR2322-30{\times}KNR2503-60(A)$ was harvested in 13.5 days after scraping old medium were selected. Crossing between A and B resulted in two high quality and speedy growing strain, A8B8 and A8B10. Their days for harvest after scraping, weight, and quality were 14.0 days, 88.8g and 92.7g, 7.0 and 7.1. Originality tests were conducted with genomic DNAs of A8B8 and Keuneutari 3ho(commercial strain) by DNA fingerprinting a confrontation culture. The results showed polymorphism and a inhibition line between them. It suggested that the new strain have originality from the commercial strain. A8B8 have been registered in Korea Seed and Variety Service with as "Saesongi 1ho".

Effects of Dietary Protein Level and Phase Feeding Regimen on Growth Performance, Carcass Characteristics and Pork Quality in Growing-finishing Pigs

  • Choi, Jae-Young;Shinde, Prashant;Jin, Zheng;Kim, Jin-Soo;Chae, Byung-Jo
    • Journal of Animal Science and Technology
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    • v.52 no.3
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    • pp.205-212
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    • 2010
  • Two experiments were conducted to study the effects of dietary protein level and phase feeding regimens on performance, carcass characteristics and pork quality in growing-finishing pigs. In both experiments, growing pigs (n=90; barrows; Landrace $\times$ Yorkshire $\times$ Duroc) were allotted to 3 treatments. Each treatment had 3 replicate pens comprising 10 pigs in each. In Exp. 1, pigs (average body weight $25.06{\pm}0.57\;kg$) were fed diets having low (17.50-14.50-12.51%), medium (19.49-17.50-13.87%) or high (22.47-19.49-16.36%) CP concentration (LP, MP and HP diets, respectively) diets during grower-finisher I-finisher II phases, respectively. Feeding of MP diet resulted in higher (P<0.05) ADG and better FCR than pigs fed LP diet. Pigs fed HP diet had lower (P<0.05) pork color and marbling score. Meat of pigs fed LP diet had higher (P<0.05) Thiobarbituric acid reactive substances (TBARS), while meat of pigs fed HP and LP diets had greater drip loss than pigs fed MP diet. In addition, meat from pigs fed LP diet was darker while meat of pigs fed HP and MP diets was paler and redder. In Exp. 2, pigs (average body weight $10.40{\pm}0.61\;kg$) were subjected during 126 days to three or four phase regimes of different duration. The treatments T1 and T2 were subjected to four phase feeding regimens (grower I, grower II, finisher I and finisher II) for 22, 42, 32 and 30 days (T1) and 40, 40, 25 and 21 days (T2), respectively, while T3 was subjected to three phase feeding regimen (grower I, grower II and finisherI) for 40, 56 and 30 days, respectively. Duration of phase feeding regimens had no effect (P>0.05) on the performance, carcass traits and meat quality of pigs. These results suggest that both high and low levels of CP have a negative effect on performance and pork quality; however, alterations in the phase feeding regimens do not have any discernible effect on the performance and pork quality in growing-finishing pigs.