Browse > Article

Screening of Fungal Strains Producing Lovastatin, an Antihypercholesterolemic Agent  

Bang, In-Young (Department of Food Science and Technology, Kyunghee University)
Whang, Seung-Whan (University of Incheon, Department of Biology)
Kim, Jung-Wan (University of Incheon, Department of Biology)
Kim, Sang-Yong (BioNgene Co., Ltd.)
Park, Cheon-Seok (Department of Food Science and Technology, Kyunghee University)
Publication Information
Korean Journal of Food Science and Technology / v.35, no.3, 2003 , pp. 442-446 More about this Journal
Abstract
Over two hundred fungal strains from Korean soil were tested for the production of cholesterol-lowering agent, lovastatin. Each fungal strain was cultivated in the rapeseedmeal production medium (RPM). After growing for 7 days, the presence of lovastatin in the culture was examined by TLC analysis and HPLC. Nine different fungal strains were determined to produce detectable amounts of lovastatin, among which one fungal strain isolated from barnyard manure of Kanghwa island produced 25.58 mg/L of lovastatin in the production medium. The morphological observation combined with the determination of 18S-rRNA sequence suggested that the selected strain belongs to a member of Aspergillus sp..
Keywords
lovastatin; Aspergillus; cholesterol;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
연도 인용수 순위
1 Sehnal, F. Hormonal role of ecdysteroids in insert larvea and during metamorphosis, pp. 271-278. In: Ecdysone: From Chemistry to Mode of Action. Koolman, J. (ed.). Thieme Medical Publishers, New York, USA (1989)
2 Endo, A., Kuroda, M. and Tanzawa, K. Competitive inhibition of 3-hydroxy-3-methylglutaryl coenzyme A reductase by ML-236A and ML-236B, fungal metabolites, having hypercholesterolemic activity. FEBS Lett. 72: 323-326 (1976)   DOI   ScienceOn
3 Alberts, A.W., Chen, J., Kuron, G., Hunt, V., Huff, J., Hoffman, C., Rothrock, J., Lopez, M., Joshua, H.M., Harris, E., Patchett, A., Monaghan, R., Currie, S., Stapley, E., Alberts-Schonberg, G., Hensens, O., Hirshfield, J., Hoogsteen, K., Liesch, J. and Springer, J. Mevinolin: a highly potent competitive inhibitor of hydroxymethylglutaryl coenzyme A reductase and a cholesterollowering agent. Proc. Natl. Acad. Sci. U.S.A. 77: 3957-3961(1980)   DOI   ScienceOn
4 Endo, A., Kuroda, M. and Tsujita, Y. ML-236A, ML-236B, and ML-236C, new inhibitors of cholesterogenesis produced by Penicillium citrinum. J. Antibiot. 29: 1346-1348 (1976)   DOI   PUBMED
5 Murray, P.R., Baron, E.J., Pfaller, M.A, Tenover, F.C and Yolken, R.H. Manual of Clinical Microbiology. pp. 699-854. American Society for Microbiology, Washington, DC, USA (1995)
6 Stryer, L. Biochemistry, 4th ed. pp. 685-712. W.H. Freeman & Company, New York, USA (1995)
7 Tobert, J.A. New developments in lipid-lowering therapy: the role of inhibitors of hydroxymethyl-glutaryl-coenzyme A reductase Circulation 76: 534-538 (1987)   DOI   ScienceOn
8 Gyorgy, S., Gyorgy, M. and Robert, P.T. Production of lovastatin by a wild strain of Aspergillus terreus. Biotechnol. Lett. 20: 411-415 (1998)   DOI   ScienceOn
9 National Statistical Office. Annual Report on the Cause of Death Statistics. National Statistical Office, Korea (1996)
10 Grover, S.A., Coupal, L. and Hu, X.P. Identifying adults at increased risk of coronary disease. J. Am. Med. Assoc. 274: 801-806 (1995)   DOI   ScienceOn
11 Sutton, D.A., Fothergill, A.W and Rinaldi, M.G. Guide to Clinically Significant Fungi. Williams & Wilkins, Baltimore, USA (1998)
12 Sitaram Kumar, M., Pallapothu, M.K., Hemant M.S and Sadhukhan, A.K. A rapid technique for screening of lovastatinproducing strains of Aspergillus terreus by agar plug and Neurospora crassa bioassay. J. Microbiol. Methods 40: 99-104 (2000)   DOI   ScienceOn
13 St-Germain, G. and Summerbell, R. Identifying Filamentous Fungi. Star Publishing Company, Belmont (1996)
14 Nina, G.C., Jozica, F., Alesksa, C. and Neda, B. Screening fungi for the production of an inhibitor of HMG CoA reductase: Production of mevinolin by the fungi of the genus Pleurotus. FEMS Microbiol. Lett. 111: 203-206 (1993)   DOI