• Journal of Microbiology and Biotechnology
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• 제8권3호
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• pp.280-283
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• 1998

We have cloned the RNA polymerase sigma-like factors from a wide range of actinomycetes by using specific primers with the polymerase chain reaction (PCR). The specific oligonucleotide primers were designed on the basis of amino acid sequences of conserved regions from HrdA, B, D of Streptomyces griseus as well as from the rpoD box of many eubacteria. The consensus sequences were from the rpoD box and helix-turn-helix motif involved in -35 recognition. The designed primers were successfully applied to amplify the DNA fragments of the hrd homolog genes from 8 different strains of actinomycetes which produce a wide variety of important antibiotics. The 480 bp of the DNA fragment was amplified from all 8 strains, and it was identified as a part of hrdA and hrdB as we designed. The deduced amino acid sequence of PCR-amplified DNA fragments were highly homologous to those of other known RNA polymerase sigma factors of S. griseus and Streptomyces aureofaciens. Therefore, this study with specifically designed primers will support rapid cloning of the RNA polymerase sigma factors which recognize different classes of promoters from actinomycetes, and it will also be helpful in understanding the relationship of promoters and sigma factors leading to heterogeneity of RNA polymerases in actinomycetes.

• 한국식품위생안전성학회:학술대회논문집
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• 한국식품위생안전성학회 2001년도 The Asia-Pacific Conference on Reproductive Biology and Environmental Sciences
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• pp.252-256
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• 2001

• 한국생물물리학회:학술대회논문집
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• 한국생물물리학회 2002년도 제9회 학술 발표회 프로그램과 논문초록
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• pp.49-49
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• 2002

Mutants blocked at the earliest stages of morphological development in Streptomyces species are called bld mutants. We have cloned bldB gene ORF from Slividans. Genomic Southern blot analysis for main strains S.lividans, S.seoulensis, S.coelicolor A3(2), and S.griseus indicated that bldB gene is conserved in all main Streptomyces strains.(omitted)

• Journal of Microbiology and Biotechnology
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• 제16권6호
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• pp.896-900
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• 2006

Cytokinin has been known to act as a plant hormone to promote cell division and function in diverse processes in plant growth and development. Besides being produced in plants, it is also produced by various bacteria and fungi; however, its ecological significance is still unclear. In this report, we present an interesting finding that transzeatin riboside (tZR), a naturally occurring cytokinin compound, increased antibiotic production in many different streptomycetes, including Streptomyces coelicolor Ml3O, S. pristinaespiralis ATCC 25486, S. violaceoruber Tu22, S. anfibioticus ATCC l1891, and S. griseus IFO 13350. In vitro plate assays showed that the addition of 100 $\mu$M tZR increased the growth inhibition of Pseudomonas syringae pv. syringae, a plant pathogen, by S. griseus, a streptomycin producer. We suggest that cytokinin could act as a signaling molecule for antibiotic production in streptomycetes, a group of rhizosphere bacteria.

• 농약과학회지
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• 제16권4호
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• pp.383-386
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• 2012

비농경지의 식물 근권토양에서 식물체의 초기생육촉진 효과가 있는 방선균을 분리하였다. 분리된 미생물의 16S rDNA 염기서열을 분석한 결과 Streptomyces spp.로 동정되었다. Streptomyces griseus (MSS181), Streptomyces griseoaurantiacus (MSS269), Streptomyces microflavus (MSS275), Streptomyces herbaricolor (MSS276)의 배양액을 오이, 고추, 담배와 토마토의 생육초기단계에 관주 처리하여 식물체의 초장, 건조중량을 측정하였다. 방선균 처리에 의해 오이의 초장은 대조구에 비해 16-29% 증가하였으나 건조중량에는 통계적으로 유의한 차이가 없었다. 같은 방선균을 고추에 처리하였을 때 고추의 초장은 대조구에 비해 10-19%, 건조중량은 19-25% 증가하였다. 담배의 건조중량은 44-73% 증가하였고 토마토의 건조중량도 65-165% 증가하였다. 공시균주의 식물병원균에 대한 항균활성을 검정한 결과, MSS275의 Phytophthora capsici, Fusarium oxysporum, Rhizoctonia solani와 Sclerotinia sclerotiorum에 대한 강한 항균활성을 확인하였다.

• 한국미생물·생명공학회지
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• 제17권5호
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• pp.529-532
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• 1989

A strain of Streptomyces sp. (YS-221-B) extracellularly produced an inhibitory substance for $\alpha$-D-Glucosidase. The substance was purified 96-fold from culture filtrate by dialysis, heat treatment, adsorption on active carbon, Bio-Gel P-10 and Sephadex G-75 column chromatography with yield of 9.2%. The substance was stable in pH range from 7.0 to 11.0 at 37$^{\circ}C$, and a treatment at 10$0^{\circ}C$ for 20 min diminished only 15% of the original activity. The inhibitor was not inactivated by the treatment of $\alpha$-, $\beta$-amylases, glucoamylases, trypsin and chymotrypsin but inactivated by pyoteases from Streptomyces griseus and Tritirachium album.

• 한국식품위생안전성학회:학술대회논문집
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• 한국식품위생안전성학회 2001년도 The Asia-Pacific Conference on Reproductive Biology and Environmental Sciences
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• pp.239-242
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• 2001

• 한국미생물·생명공학회지
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• 제30권2호
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• pp.105-110
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• 2002

The sequence of 3,221 nucleotides immediately adjacent to rpsA gene encoding 30S ribosomal protein S1 of Brevibacterium ammoniagenes was determined. A putative open reading frame (ORF) of 2,670 nucleotides for a polypeptide of 889 amino acid residues and a TAG stop codon was found, which is located at a distance of 723 nucleotides upstream from rpsA gene with same translational direction. The deduced amino acid sequence of the ORF was found to be highly homologous to the DNA polymerase I of Streptomyces griseus (75.48％), Rhodococcus sp. ATCC 15963 (56.69％), Mycobacterium tuberculosis (55.46％) and Mycobacterium leprae (53.99％). It was suggested that the predicted product of the ORF is a DNA polymerase I with three functional domains. Two domains of 5 → 3 exonuclease and DNA polymerase are highly conserved with other DNA polymerase I, but 3 → 5 exonuclease domain is less conserved.

• Journal of Microbiology and Biotechnology
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• 제28권11호
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• pp.1876-1882
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• 2018

A series of pentacyclic triterpenoids similar to glycyrrhetinic acid were designed and synthesized via the combination of chemical modification and microbial catalysis. All products were screened for the glycogen phosphorylases inhibitory activities in vitro. Within this series of derivatives, compound 5 displayed good inhibitory activities with $IC_{50}$ value of $27.7{\mu}M$, which is better than that of the other derivatives and glycyrrhetinic acid. Structure-activity relationship (SAR) analysis of these inhibitors was also discussed.

• 한국동물학회지
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• 제17권1호
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• pp.37-42
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• 1974

Chinese hamster, Cricetulus griseus 와 Armenian hamster, Cricetulus migratorius의 正常細胞, 腫瘍瘤에서 由來된 細胞 및 바이러스로 再形質轉換된 細胞의 乳酸데히드로게나제(LDH) 패턴을 電氣永動法에 의해 測定하여 比較하였다. 이 두 종류의 電基泳動移動度는 비슷했으며, 纖維芽細胞는 LDH-5만 나타내었고, adenovirus로 形質轉換된 上皮性인 細胞에서는 LDH-1-2-3-4-5, 2-3-4-5, 또는 3-4-5와 같은 패턴을 보여 주었다. 그러나 上皮性細胞가 自然的으로, 혹은 SV40 바이러스 處理로 因해서 纖維芽細胞로 변하였을 경우는 역시 LDH-5 패턴만 보여 주었다.