• The Korean Journal of Pesticide Science
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• v.18 no.4
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• pp.321-329
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• 2014

This experiment was conducted to establish an analytical method for residues of amisulbrom, as recently developed an oomycete-specific fungicide showing inhibition of fungal respiration, in crops using HPLC-UVD/MS. Amisulbrom residue was extracted with acetonitrile from representative samples of five raw products which comprised apple, green pepper, kimchi cabbage, potato and hulled rice. The extract was diluted with 50 mL of saline water and directly partitioned into dichloromethane to remove polar co-extractives in the aqueous phase. For the hulled rice sample, n-hexane/acetonitrile partition was additionally employed to remove non-polar lipids. The extract was finally purified by optimized Florisil column chromatography. On an octadecylsilyl column in HPLC, amisulbrom was successfully separated from sample co-extractives and sensitively quantitated by ultraviolet absorption at 255 nm with no interference. Accuracy and precision of the proposed method was validated by the recovery test on every crop samples fortified with amisulbrom at 3 concentration levels per crop in each triplication. Mean recoveries ranged from 85.3% to 105.6% in five representative agricultural commodities. The coefficients of variation were all less than 10%, irrespective of sample types and fortification levels. Limit of quantitation (LOQ) of amisulbrom was 0.04 mg/kg as verified by the recovery experiment. A confirmatory method using LC/MS with selected-ion monitoring technique was also provided to clearly identify the suspected residue. The proposed method was sensitive, reproducible and easy-to-operate enough to routinely determine the residue of amisulbrom in agricultural commodities.

• Journal of The Korean Society of Grassland and Forage Science
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• v.28 no.3
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• pp.177-184
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• 2008

This experiment was carried out to breed a cold-tolerant variety of Italian ryegrass (Lolium multiflorum Lam.) in Grassland and Forage Crops Division, National Institute of Animal Science, RDA, Suwon from 1999 to 2006. ew variety, Kowinmaster as a diploid variety was light-green in leaf color and had medium and semi-erect growth habit in late autumn and early spring, respectively. Kowinmaster was 13th May in heading date as a medium-maturing variety. Especially, Kowinmaster was similar to Florida 80 in flag leaf width, longer 3cm in flag leaf length and longer 6cm in plant length than those of control variety, Florida 80, respectively. Stem width of Kowinmaster was similar to that of Florida 80. Cold tolerance of Kowinmaster was better than that of Florida 80. Dry matter yield (9,521 kg/ha) of Kowinmaster was 2% more than that of Florida 80. Feed value of Kowinmaster was 12.1% in crude protein, 70.9% in In vitro dry matter digestibility and 32.9% in acid detergent fiber that were similar to those of Florida 80, respectively, but in neutral detergent fiber, Kowimaster was 56.0% that was 1% lower than that of Florida 80.

• The Korean Journal of Pesticide Science
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• v.21 no.1
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• pp.75-83
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• 2017

The single residue analytical method was developed for determining fungicide pencycuron residues in various agricultural commodities with high-performance liquid chromatography (HPLC). Pencycuron residue was extracted with acetone from representative crops such as Korean cabbage, apple, brown rice and green pepper. After ethyl acetate/n-hexane partition and subsequent clean-up with silica gel chromatography, pencycuron residue was quantified by reversed phase HPLC with UV detection at 240 nm. The suspected residue of pencycuron was confirmed using selected-ion monitoring (SIM) LC/mass spectrometry (MS). Instrumental limit of quantitation (ILOQ) and method LOQ (MLOQ) were set at 2 ng and 0.02 mg/kg, respectively. Overall recoveries of pencycuron from different crop samples fortified at three levels (MLOQ, 10MLOQ, 100MLOQ) were 72~108%. This proposed method could be useful as official analytical method for quantification of pencycuron residues in agricultural commodities.

• Korean Journal of Soil Science and Fertilizer
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• v.40 no.4
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• pp.249-254
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• 2007

Inoculation dose of agriculturally important microbes is an important criterion that decides the establishment and hence their effects on plant growth. Effects of the inoculation dose of Azospirillum brasilense strain CW903 on the growth and nutrient absorption of three different crops, tomato, rice and red pepper were assessed under green house condition. Three different concentrations of A. brasilense strain CW903 ($10^5$, $10^6$ and $10^8cfu\;mL^{-1}$) were applied through seed treatment and through the soil near the root zone (1 mL per plant) at 20 and 30 days after sowing. Positive effects on the growth of tomato, rice and red pepper were found at $10^6$ and $10^8cfu\;mL^{-1}$ inoculation doses of A. brasilense strain CW903. The inoculation dose of $10^8cfu\;mL^{-1}$ of A. brasilense strain CW903 recorded the best effects on growth parameters like shoot and root length and the absorption of important nutrients.

• Journal of Bio-Environment Control
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• v.24 no.4
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• pp.271-274
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• 2015

The objective of this study was to investigate the effects of composition of nutrient solution on the growth and quality of baby leaf vegetables (tat soi, romaine lettuce, beet, and red radish) hydroponically cultivated in plant factory. The seeds of four vegetable crops were sown in urethane sponges and cultivated for 14 days in a plant factory. Light intensity and photoperiod were $110{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$ and 16 h, respectively; and air temperature in photo/darkperiod was maintained at $25/20^{\circ}C$. Tap water was used for irrigation for 7 days after sowing, and then plants were irrigated for 7 days using tap water and the nutrient solutions of Korea Wonshi, Japan Enshi, and Yamazaki for lettuce. At 14 days after sowing, the fresh weight of tah soi was highest in the nutrient solution of Yamazaki for lettuce, and there were no significant differences among nutrient solutions in beet and red radish. When we compared leaf color using Hunter's a value, the nutrient solution of Korea Wonshi and Japan Enshi increased green color in baby leaf vegetables, while the nutrient solution of Yamazaki for lettuce increased red color. Total phenolic content of romaine lettuce was highest in the nutrient solution of Korea Wonshi, but tat soi, beet, and red radish showed no significant differences among nutrient solutions in total phenolic contents. From these results, we suggest that using the nutrient solution of Korea Wonshi can enhance the growth and quality of romaine lettuce and the nutrient solution of Yamazaki for lettuce is appropriate for enhancing the growth and red color of beet and red radish in plant factory.

• Journal of Plant Biotechnology
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• v.45 no.3
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• pp.190-195
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• 2018

Sweetpotato [Ipomoea batatas (L.) Lam] represents an attractive starch crop that can be used to facilitate solving global food and environmental problems in the $21^{st}$ century. It can be used as industrial bioreactors to produce various high value-added materials, including bio-ethanol, functional feed, antioxidants, as well as food resources. The non-profit Center for Science in the Public Interest (CSPI) announced sweetpotato as one of the ten 'super foods' for better health, since it contains high levels of low molecular weight antioxidants such as vitamin-C, vitamin-E and carotenoids, as well as dietary fiber and potassium. The United States Department of Agriculture (USDA) also reported that sweetpotato is the best bioenergy crop among starch crops on marginal lands, that does not affect food security. The Food and Agriculture Organization (FAO) estimated that world population in 2050 will be 9.7 billion, and require approximately 1.7 times more food than today. In this respect, sweetpotato will be a solution to solving problems such as food, energy, health, and environment facing the globe in the $21^{st}$ century. In this paper, the current status of resources, and cultivation of sweetpotato in the world was first described. Development of a new northern route of the sweetpotato and its prior tasks of large scale cultivation of sweetpotato, were also described in terms of global food security, and production of high-value added biomaterials.

• Proceedings of the Korean Society of Crop Science Conference
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• 2017.06a
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• pp.7-7
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• 2017

Unfavorable weather and soil conditions reduce rice yield and land and water productivity, aggravating existing encounters of poverty and food insecurity. These conditions are foreseen to worsen with climate change and with the unceasing irrational human practices that progressively debilitate productivity despite global appeals for more food. Our understanding of plant responses to abiotic stresses is advancing and is complex, involving numerous critical processes - each controlled by several genetic factors. Knowledge of the physiological and molecular mechanisms involved in signaling, response and adaptation, and in some cases the genes involved, is advancing. Moreover, the genetic diversity being unveiled within cultivated rice and its wild relatives is providing ample resources for trait and gene discovery, and this is being scouted for rice improvement using modern genomics and molecular tools. Development of stress tolerant varieties is now being fast-tracked through the use of DNA markers and advanced breeding strategies. Large numbers of drought, submergence and salt tolerant varieties were commercialized over recent years in South and Southeast Asia and more recently in Africa. These varieties are making significant changes in less favorable areas, transforming lives of smallholder farmers - progress considered incredulous in the past. The stress tolerant varieties are providing assurance to farmers to invest in better management of their crops and the ability to adjust their cropping systems for even higher productivity and more income, sparking changes analogous to that of the first green revolution, which previously benefited only favorable irrigated and rainfed areas. New breeding tools using markers for multiple stresses made it possible to develop more resilient, higher yielding varieties to replace the aging and obsolete varieties still dominating these areas. Varieties with multiple stress tolerances are now becoming available, providing even better security for farmers and lessening their production risks even in areas affected by complex and overlapping stresses. The progress made in these less favorable areas triggered numerous favorable changes at the national and regional levels in several countries in Asia, including adjusting breeding and dissemination strategies to accelerate outreach and enabling changes at higher policy levels, creating a positive environment for faster progress. Exploiting the potential of these less productive areas for food production is inevitable, to meet the escalating global needs for more food and sustained production systems, at times when national resources are shrinking while demand for food is mounting. However, the success in these areas requires concerted efforts to make use of existing genetic resources for crop improvement and establishing effective evaluation networks, seed production systems, and seed delivery systems to ensure faster outreach and transformation.

• Research in Plant Disease
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• v.26 no.1
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• pp.8-18
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• 2020

Red pepper, one of the major economic crops in Korea, is being affected by anthracnose disease caused by Colletotrichum acutatum. To control this disease, an antagonistic bacterial strain, Bacillus subtilis YGB36 identified by 16S rDNA sequencing, physiological and biochemical analyses is used as a biological control agent. In vitro screening revealed that the strain YGB36 possess strong antifungal activity against the pathogen Cylindrocarpon destructans. The strain exhibited cellulase, protease, amylase, siderophore production and phosphate solubility. In vitro conidial germination of C. acutatum was most drastically inhibited by YGB36 cell suspensions (10⁶ cfu/ml) or culture filtrate. Development of anthracnose symptoms was reduced on detached immature green pepper fruits by treatment with cell suspensions, and its control value was recorded as 65.7%. The YGB36 bacterial suspension treatment enhanced the germination rate of red pepper seeds and promoted root development and growth under greenhouse conditions. The in vitro screening of fungicide and insecticide sensitivity test against YGB36 revealed that the bacterial growth was not affected by any of the insecticides, and 11 fungicides out of 21 used. Collectively, our results clearly suggest that the strain YGB36 is considered as one of the potential biocontrol agents against anthracnose disease in red pepper.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.21 no.4
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• pp.218-225
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• 2020

This study analyzed the priorities of the cross-compliance items of public-benefit direct payment using an Analytic Hierarchy Process and Fuzzy Decision Making Analysis. The valuation criteria are policy efficiency, farm acceptability, and feasibility, and the valuation targets are the basic and additional cross-compliance items. The survey was performed by targeting 50 experts from each class, and conducted for about a month starting from the beginning of July 2019. The results show that the weight of the valuation criteria is higher in the order of farm acceptability, feasibility, and policy efficiency. Compliance with PLS standards, compliance with disposal standards of waste vinyl and pesticides, soil testing, compliance with toxic substance standards, education, etc. are comparatively evaluated to be higher cross-compliance items in basic cross-compliance. Disposing of an abandoned well, jointly collecting and disposing of agricultural by-products, common area care and cleaning, maintenance of empty houses and poor facilities, growing green manure crops during the fallow period, etc. are comparatively evaluated to be higher cross-compliance items for the additional cross-compliance. The results of this study are expected to contribute to the government's policy related to the cross-compliance of public-benefit direct payment.

• The Korean Journal of Pesticide Science
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• v.8 no.4
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• pp.280-287
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• 2004

We investigated the residual amounts of endosulfan and procymidone taken by vegetables grown in hydroponics culture and field conditions treated with the pesticides in order to evaluate safe cultivation concentration of the pesticides in the vegetables. Endosulfan and procymidone were selected as test pesticides because they have been reported to frequently detect in agricultural products at different concentrations. In hydroponic culture, by Chinese cabbage, procymidone was absorbed 3.8 times higher than endosulfan. The higher the pesticide concentration get, the worse the plant grew. In soil treated with 10 mg/kg of endosulfan, the pesticide absorbed by Leafy radish, Chinese cabbage and radish was less then their MRLs. In case of carlot, the residue level in soil which did not exceed its MRL was 1 mg/kg. The concentrations of procymidone in soil which did not exceed the MRLs in Leafy radish, Chinese cabbage, radish and carrot were 10, 10, 2 and 1 mg/kg, respectively Usually aged endosulfan and procymidone residues were less absorbed into crops than the fresh ones. Chinese cabbage absorbed more endosulfan and pocymidone than leafy radish, radish doing more than carrot.