Research in Plant Disease (식물병연구)

The Korean Society of Plant Pathology (한국식물병리학회)
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Control of Red Pepper Anthracnose Using Bacillus subtilis YGB36, a Plant Growth Promoting Rhizobacterium

식물생장촉진근권세균 Bacillus subtilis YGB36을 이용한 고추 탄저병의 생물학적 방제

  • Lee, Yong Yoon (Department of Plant Medicals, Andong National University) ;
  • Lee, Younmi (Agricultural Science & Technology Research Institute, Andong National University) ;
  • Kim, Young Soo (Department of Plant Medicals, Andong National University) ;
  • Kim, Hyun Sup (Department of Plant Medicals, Andong National University) ;
  • Jeon, Yongho (Department of Plant Medicals, Andong National University)
  • 이용윤 (안동대학교 식물의학과) ;
  • 이연미 (안동대학교 농업과학기술연구소) ;
  • 김영수 (안동대학교 식물의학과) ;
  • 김현섭 (안동대학교 식물의학과) ;
  • 전용호 (안동대학교 식물의학과)
  • Received : 2020.02.06
  • Accepted : 2020.03.09
  • Published : 2020.03.31
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Abstract

Red pepper, one of the major economic crops in Korea, is being affected by anthracnose disease caused by Colletotrichum acutatum. To control this disease, an antagonistic bacterial strain, Bacillus subtilis YGB36 identified by 16S rDNA sequencing, physiological and biochemical analyses is used as a biological control agent. In vitro screening revealed that the strain YGB36 possess strong antifungal activity against the pathogen Cylindrocarpon destructans. The strain exhibited cellulase, protease, amylase, siderophore production and phosphate solubility. In vitro conidial germination of C. acutatum was most drastically inhibited by YGB36 cell suspensions (106 cfu/ml) or culture filtrate. Development of anthracnose symptoms was reduced on detached immature green pepper fruits by treatment with cell suspensions, and its control value was recorded as 65.7%. The YGB36 bacterial suspension treatment enhanced the germination rate of red pepper seeds and promoted root development and growth under greenhouse conditions. The in vitro screening of fungicide and insecticide sensitivity test against YGB36 revealed that the bacterial growth was not affected by any of the insecticides, and 11 fungicides out of 21 used. Collectively, our results clearly suggest that the strain YGB36 is considered as one of the potential biocontrol agents against anthracnose disease in red pepper.

고추는 한국의 주요 경제 작물 중 하나이지만 재배과정에서 Colletotrichum acutatum에 기인한 탄저병이 많이 발생한다. 이의 방제를 위해 길항균인 Bacillus subtilis YGB36을 선발하여 16S rRNA 염기서열 및 생리·생화학적 분석을 통해 Bacillus subtilis로 동정하고 이를 생물학적 방제제로 개발하고자 실험을 진행하였다. In vitro screening에서 YGB36은 Cylindrocarpon destructans에 강한 항균활성을 나타내었고, cellulase, protease, amylase, siderophore 생산 및 phosphate solubility를 보유하고 있었다. 이 균주의 배양액(106 cfu/ml) 및 배양여액은 in vitro 실험에서 C. acutatum의 포자발아를 강하게 억제하였고, 고추 과실을 이용한 실내실험에서도 이 배양액이 탄저병 병반의 진전을 억제하였으며, 방제가는 65.7%로 나타났다. 아울러 YGB36배양액은 고추 종자의 발아 및 초기 뿌리 생장을 촉진하였고, 온실에서의 식물 생장을 촉진하였다. 기존 화학농약과의 혼용 가능성 조사 결과, YGB36은 실험에 사용한 모든 살충제에서 생존에 영향을 전혀 받지 않았으며, 살균제 21종 중 11종에서 잘 생존하였다. 따라서 YGB36균주는 고추 탄저병에 대한 생물농약으로서의 잠재적 가치가 상당한 것으로 판단된다.

Keywords

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