• 한국생물공학회:학술대회논문집
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• 한국생물공학회 2000년도 춘계학술발표대회
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• pp.497-500
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• 2000

유류오염토양에서 분리한 유류분해균주 중 계면장력 저하능이 우수한 균주를 분리하여 동정한 결과 Pseudomonas aeruginosa D2D2로 판명되었다. 탄소원으로 glucose와 olive oil을 각 1.5%씩 첨가하였을 때 표면장력이 27.9 dyne/cm로 표면장력 저하능력이 가장 우수한 것으로 나타냈다. 생산된 biosurfactant로 ethly acetate 추출하여 crude biosurfactant를 얻었으며, TLC 분석한 결과 glycolipid계열의 biosurfactant임을 확인하였다.

• Applied Biological Chemistry
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• 제17권2호
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• pp.138-142
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• 1974

유산염배지(乳酸鹽培地)에서 배양(培養)한 Selenomonas ruminantium 균체(菌體)를 초음파처리후(超音波處理後) 추출(抽出)한 효소(酵素)와 cofactor로서 ATP, CoA, $Mg^{++}$, UTP를 사용(使用)하여 in vitro에서 당지질(糖脂質)을 생합성(生合成)하여 본 결과(結果)는 다음과 같다. 1. Selenomonas ruminantium의 효소(酵素)에 의(依)한 $^{14}C-glucosamine$과 tridecyl-Co A로 부터의 당지질(糖脂質)의 in vitro 생합성(生合成)은 ATP, $Mg^{++}$, UTP,에 의(依)하여 촉진(促進)되었다. 2. 효소추출액(酵素抽出液)을 원심분리(遠心分離)로 분획(分劃)하고 각분획(各分劃)의 glycolipid 합성능(合成能)을 본 결과(結果) 105,000의 supernatant에 6,000rpm supernatant의 약(約) 2배(倍)의 효소활성(酵素活性)을 나타내는 조효소(粗酵素)가 존재(存在)함을 알았다. 3. 효소반응(酵素反應)은 30분(分)일 때 대부분(大部分)이 진행(進行)되며 1시간(時間)에서 거의 완결(完結)됨을 알았다.

• 한국정보통신학회:학술대회논문집
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• 한국정보통신학회 2015년도 춘계학술대회
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• pp.943-946
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• 2015

탈수초화를 위해 쥐의 배아(임신 16일)의 슈반세포와 뉴런 세포가 척수신경절로 부터 in vitro 시스템에서 배양되었다. 항 유사분열제로 첨가된 분리 정제된 척수신경절 세포와 분리 정제된 슈반세포가 공동배양 배양되었고 배양되었고 수초화 과정이 구축되었다. 이렇게 형성된 공동 배양에 M. leprae-specific phenolic glycolipid-1 (PGL-1)을 처리하고 myelin basic protein (MBP)의 항체를 이용하여 탈수초화가 형성되었음을 확인하였다.

• 한국축산식품학회지
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• 제18권4호
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• pp.332-338
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• 1998

This experiment was carried out to investigate the changes in phospholipid and glycolipid of Kaesojoo and Kaesojoo added medicinal herbs during storage(30 days) at 4$^{\circ}C$and -18$^{\circ}C$. Two dogs with 12kg live weight(♀, The Korean Jindo dog Hy-breed, 11∼12 month) were slaughtered to obtain samples. The result obtained were as follows: The saturated fatty acids found in phospholipid of Kaesojoo were palmitic acid, stearic acid, myristic acid, while the unsaturated fatty acid found in phospholipid of Kaesojoo with medicinal herbs were palmitic acid, stearic acid, arachidonic acid, while the unsaturated fatty acid were mostly oleic acid, increased during the period of storage. The saturated fatty acid found in glycolipid obtained from Kaesojoo were mostly oleic acid, linoleic acid and linolenic acid. The saturated fatty acid obtained from Kaesojoo with medicinal herbs were palmitic acid, stearic acid, while the unsaturated fatty acid were mostly oleic acid, linoleic acid and linolenic acid. The rates of changes in unsaturated and saturated fatty acid were higher at 4$^{\circ}C$ than that of -18$^{\circ}C$ during storage.

• Natural Product Sciences
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• 제2권2호
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• pp.102-105
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• 1996

A new glycolipid, 2-hydroxy-3 $(octadecyloxy)-propyl-{\alpha}-D-arabinopyranoside$ (1) has been isolated from Lobophytum microlobulatum and its structure has been elucidated by physical and spectral $(UV,\;IR,\;^1H,\;^{13}C\;NMR,\;FABMS)$ data.

• 동아시아식생활학회지
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• 제3권1호
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• pp.61-66
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• 1993

The contents of crude fat were remarkably different according to parts in porcine tissues. The most contents of total lipids in variety tissues were neutral lipid, and the nexts were phospholipid and glycolipid, where these of the most composition in liver were phospholipid and the next neutrallipid and glycolipid. The major component of the nonpolar lipids in fresh was triglyceried(58.09∼74.68%) and phospolipid in the polar lipid were phosphatidyl choline(20.14∼45.17%) and phosphatidyl ethanolamine(18.5∼30.14%) and the main component in glycolipid acylsterol glyceride(26.10∼45.15%), respectively.

• Journal of Microbiology and Biotechnology
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• 제20권8호
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• pp.1240-1242
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• 2010

We attempted to identify the compound responsible for the growth inhibition of Microcystis aeruginosa occurring when a culture broth of Bacillus subtilis C1 was added to the medium. The active compound was purified from B. subtilis C1 culture broth by adsorption chromatography and HPLC, and was identified as a type of glycolipid based on $^1H$ NMR and MS analyses. The purified active compound completely inhibited the growth of M. aeruginosa at a concentration of 10 ${\mu}g/ml$. This is the first report of a glycolipid produced by a Bacillus strain that has potential as an agent for the selective control of bloom-forming M. aeruginosa.

• Journal of Microbiology and Biotechnology
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• 제12권3호
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• pp.371-376
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• 2002

A biosurfactant-producing bacterial strain was selected from diesel-contaminated soil by measuring the oil-film collapsing activity and identified as Pseudomonas aeruginosa D2D2. When glucose and olive oil were used as carbon sources, 11.46 g/1 of biosurfactant was obtained. Based on TLC analysis, the biosurfactant produced from P. aeruginosa D2D2 was identified as a glycolipid, consisting of two types of biosurfactants (Type I and Type II). The purified glycolipid reduced the surface tension of the culture from 72 dyne/cm to 27 dyne/cm. The hydrophilic and hydrophobic moiety of the biosurfactant were rhamnose and ${\beta}$-hydroxydecanoic acid, as determined by FAB-MS and NMR analyses, respectively.

• 한국가금학회지
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• 제15권3호
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• pp.247-251
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• 1988

본 실험은 같은 조건에서 8주령까지 사육된 부로일러 암ㆍ수의 가슴과 다리근육의 지질, 지폐산 및 총 Clesterol함량을 분석 비교한 것으로 그 결과는 다음과 같다. 암ㆍ수 부로일러 모두 가슴근육이 다리근육에 비하여 총지질과 중성지질 함량이 낮았으며 인지질과 당지질 함량은 많았다. 부로일러육의 불포화지방산 조성비는 중성지질과 인지질은 다리근육, 당지질은 가슴근육이 높았다. 암컷은 수컷에 비하여 당지질의 불포화지방산 비율이 낮았다. 암컷은 수컷에 비하여 중성지질의 palmitoleic acid, 인지질의 palmitoleic, stearic. linolenic, arachidic 및 arachidonic acid 그리고 당지질의 palmitoleic acid와 stearic acid가 많았다. 총 cholesterol 은 지방조직을 제거한 근육은 수컷의 다리, 지방조직을 제거하지 않은 것은 암컷의 다리에서 가장 높은 함량을 보였다.

• Applied Biological Chemistry
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• 제27권3호
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• pp.211-213
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• 1984

The change in fatty acid compositions of the glycolipid and phospholipid of sesame seed was analyzed during the storage for 4, 9, and 15 months at four different storage conditions, that is, storage under light with linen bag(LA), storage under light with polyethylene bag(LS), storage in dark with linen bag(DA), and storage in dark with polyethylene bag(DS). In the fatty acid composition of glycolipid, palmitic and stearic acid decreased during the storage but the linoleic acid increased significantly, particularly, between 4 to 9 months of storage. The content of palmitic and stearic acid in phospholipid decreased until 9 months, then increased until 15 months of storage, but reverse tendency was, observed in the content of oleic and linoleic acid. The ratio of unsaturated to saturated fatty acids in glycolipid increased during the storage of 15 months in all the storage conditions. The ratio of unsaturated to saturated fatty acids of phospholipid in DA and LA was slightly higher than that in DS and LS conditions during the storage for 15 months.