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Glycolipid Biosurfactants Produced by Pseudomonas aeruginosa D2D2 from Diesel-Contaminated Soil  

MOON, HYE-JOON (Research Institute for Clean Technology, Myong-Ji University)
YOUNG-KUONG LIM (Department of Environmental Engineering and Biotechnology, Myong-Ji University)
HEE-SIK KIM (Environmental Microbiology R.U., Korea Research Institute for Bioscience and Biotechnology)
DAE-YOUNG KWON (Department of Environmental Engineering and Biotechnology, Myong-Ji University)
WOOK-JIN CHUNG (Department of Environmental Engineering and Biotechnology, Myong-Ji University)
Publication Information
Journal of Microbiology and Biotechnology / v.12, no.3, 2002 , pp. 371-376 More about this Journal
Abstract
A biosurfactant-producing bacterial strain was selected from diesel-contaminated soil by measuring the oil-film collapsing activity and identified as Pseudomonas aeruginosa D2D2. When glucose and olive oil were used as carbon sources, 11.46 g/1 of biosurfactant was obtained. Based on TLC analysis, the biosurfactant produced from P. aeruginosa D2D2 was identified as a glycolipid, consisting of two types of biosurfactants (Type I and Type II). The purified glycolipid reduced the surface tension of the culture from 72 dyne/cm to 27 dyne/cm. The hydrophilic and hydrophobic moiety of the biosurfactant were rhamnose and ${\beta}$-hydroxydecanoic acid, as determined by FAB-MS and NMR analyses, respectively.
Keywords
Biosurfactant; glycolipid; Pseudomonas aeruginosa D2D2; rhamnolipid;
Citations & Related Records
Times Cited By KSCI : 5  (Citation Analysis)
Times Cited By Web Of Science : 7  (Related Records In Web of Science)
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