• 대한화학회지
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• 제21권3호
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• pp.210-214
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• 1977

한국산 밀 품종들 중에서 장광의 것을 선정하여 단백질을 추출한 다음 이 단백질의 아미노산 조성과 gliadin 단백질의 정제로 주 gliadin 단백질을 분리하여 본 결과 1. Gliadin과 glutenin 단백질은 다같이 disc 전기이동상에 5개의 band를 나타내었다. 2. Gliadin과 glutenin 단백질은 다같이 glutamic acid의 함량이 가장 많았다. 3. Gliadin과 glutenin 단백질간에 아미노산 조성의 차이는 gliadin이 glutenin보다 alanine의 함량은 적었으나 proline의 함량은 많았다. 4. 주 gliadin 단백질은 gliadin 단백질을 Sephadex G-150과 A-25 coumn으로 정제하여 얻었다.

• 좋은식품
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• 통권19호
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• pp.15-19
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• 1974

밀가루 단백질인 Gluten은 밀가루 반죽의 부피를 크게하는 중요한 역할을 하며 이 Gluten 단백질을 구성한 Gliadin의 분자량은 40,000이며 Glutenin은 2$\~$3백만의 고분자화합물로 Gliadin과 Glutenin은 서로 구조상으로는 다르나 Gliadin이 Glutenin 구성 polypeptide로 S-S 결합에 중합체로 구성하며 이들은 20,000$\~$25,000정도의 공통된 polypeptide로부터 이루어진 것으로 추리하고 있다. Gluten 단백질에 S-S 함량이 7.4$\~$10mole/$10^5$g protein이며 -SH 함량은 0$\~$0.3mole/$10^5$g protein이다. Gluten 표면에 -SH가 적으나 S-S, 및 -SH의 상호교환이 일어나 망상 형성하여 밀가루 반죽내에서 발생하는 $CO_2$의 가스를 들어쌓여 빵의 부피를 크게 한 것이다.

• 한국작물학회지
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• 제54권2호
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• pp.181-191
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• 2009

To investigate the genetic variation of high-and low-molecular-weight glutenin subunits (BMW-GS and LMW-GS), granule-bound starch synthase I (GBSSI) and puroindoline in 24 Korean wheat cultivars. At the BMW-GS compositions, three Glu-A1 alleles, five Glu-B1 alleles and three Glu-D1 alleles were identified. The high frequency of alleles at each locus was Glu-A1c allele (15 cultivars), Glu-B1b allele (16 cultivars) and Glu-D1f allele (16 cultivars). Four alleles were identified at the Glu-A3 and Glu-B3 loci and three at Glu-D3 locus and Glu-A3d, Glu-B3d and Glu-D3a were mainly found at each Glu-3 locus. Glu-A3d, Glu-B3d, Glu-D3b or c (4 cultivars, respectively) and Glu-A3d, Glu-B3d, Glu-D3a and Glu-A3c, Glu-B3d or h, Glu-D3a (3 cultivar, respectively) were predominantly found in Korean wheats. At the GBSS compositions, 2 waxy wheat cultivars, Shinmichal and Shinmichal1, showed null alleles on the Wx loci and other cultivars were wild type in GBSS compositions. At the puroindoline gene compositions, Korean wheat cultivars carried 3 genotypes, which 10 cultivars (41.7%) were Pina-D1a and Pinb-D1a, 11 cultivars (45.8%) had Pina-D1a and Pinb-D1b and 3 cultivars (12.5%) carried Pina-D1b and Pinb-D1a. These genetic variations could present the information to improve flour and end-use quality in Korean wheat breeding programs.

• 한국육종학회지
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• 제43권5호
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• pp.479-489
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• 2011

Gluten is the main functional component of wheat, and is the main source of the viscoelastic properties in a dough. One of the gluten group is glutenin, which is composed of high molecular weight (HMW) and low molecular weight (LMW) subunits. The HMW glutenin subunits (HMW-GS) have been shown to play a crucial role in determining the processing properties of the grain. They are encoded by the Glu-1 loci located on the long arms of homeologous group one chromosomes, with each locus comprising two genes encoding x- and y-type subunits. The presence of certain HMW subunits is positively correlated with good bread-making quality. The highly conserved N- and C- terminal contaning cystein residues which form interand intra-chain disulphide bonds. This inter chain disulphide bonds stabilize the glutenin polymers. In contrast, the repetitive domains that comprise the central part of the HMW-GS are responsible for the elastic properties due to extensive arrays of interchain hydrogen bonds. In this review, we discuss HMW-GS, HMW-GS structure and gluten elasticity, relationship between HMW-GS and bread wheat quality and genetic engineering of the HMW-GS.

• 한국작물학회지
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• 제43권4호
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• pp.259-263
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• 1998

High molecular weight glutenin (HMW-Glu) subunit compositions of 73 Korean wheat cultivars and experimental lines were evaluated by using one dimensional sodium dodecyl sulfate-polyacrylamide gel electrophoresis. This method is suitable for obtaining a good resolution of 1Dx2 and 1Ax2$^*$ without adverse effects on separation of other HMW-Glu subunits. Korean wheats examined in this study could be divided into 15 different groups on the basis of HMW-Glu subunit compositions. From the wheat lines tested, it was identified that there were three alleles at the Glu-Al, five at the Glu-Bl and three at the Glu-D1 loci. The null allele of the Glu-Al was occurred in high frequency (79.4%), while low frequencies for 1Ax1 (12.3%) and 1Ax2$^*$(8.2%) were found. High frequency (75.3%) of the subunit pairs of 1Bx7+1By8 at the Glu-Bl loci compared with other subunits was found. The frequencies of subunits 1Dx2. 2+1Dy12 and 1Dx2+1Dy12 from the Glu-D1 loci were 54. 8% and 37.0%, respectively. However, a few Korean wheat lines (8.2%) carried 1Dx5 + 1Dy10 subunit pair which are responsible for good breadmaking quality. The information of HMW-Glu subunit compositions provide a useful tool to characterize wheat lines, and can be directly used in selection of breeding lines of different end-use properties.

• Preventive Nutrition and Food Science
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• 제11권3호
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• pp.243-252
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• 2006

White salted noodles and pan bread were prepared from Korean wheats with 1Dx2.2+1Dy12 subunits in high molecular weight glutenin subunits (HMW-GS) to evaluate the suitability for end-use products through the comparison with US wheats with various classes and commercial wheat flours. Korean wheat flours with 1Dx2.2+1Dy12 subunits showed higher SDS sedimentation volume than US wheat flours with similar protein content. Compared to wheat flours with similar protein content and SDS sedimentation volume, water absorption percent of Korean wheat flours using a mixograph was higher than that of US wheat flours, but similar to commercial wheat flours. Mixograph mixing time was similar to hard wheat flours and commercial noodle flours. Optimum water absorption percent of noodle dough from Korean wheat flours was higher than that of US wheat flours. Noodle sheets from Korean wheat flours with 1Dx2.2+ 1Dy12 subunits showed lower L values, higher a values and similar b values compared to commercial noodle flours. Hardness of cooked noodles from Korean wheat flours 1Dx2.2+1Dy12 subunits correlated positively with protein content, NIRS hardness, mixograph water absorption and gluten yield of flours. Korean wheat flours with 1Dx2.2+1Dy12 subunits showed lower loaf volume and harder crumb firmness than hard wheat flours and commercial bread wheat flours in spite of similar protein quantity and quality to hard wheat flours.

• 한국작물학회:학술대회논문집
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• 한국작물학회 1999년도 춘계 학술대회지
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• pp.57-58
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• 1999

• 한국작물학회:학술대회논문집
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• 한국작물학회 1998년도 춘계 학술대회지
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• pp.67-68
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• 1998

• Journal of Applied Biological Chemistry
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• 제59권1호
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• pp.1-3
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• 2016

Wheat is a staple food in the Korean diet, which is increasingly becoming westernized. Because most domestic wheat consumption relies on imported wheat, we aimed to evaluate the allergy-inducible protein contents of commercial flours from imported and domestic wheat. Analysis of the protein contents by densitometry suggested that domestic wheat flours contain lower levels of high molecular weight glutenin and omega-gliadin (50 and 34% lower, respectively) than imported wheat flours. Therefore, domestic wheat flours are less likely to cause allergic reactions than imported wheat flours are. Based on the findings of our study, were commend increased consumption of domestic wheat flours to those who are sensitive to allergy.

• 한국식품과학회지
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• 제31권3호
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• pp.586-592
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• 1999

그루, 은파 및 탑동 등의 한국산 소맥분의 단백질 함량은 수입 소맥과 비교하여 상당히 많은 양 함유되어 있으나 탑동을 제외한 한국밀의 반죽 물성은 매우 약하여 단백질의 함량이 많음에도 불구하고 연질맥과 같은 반죽물성을 나타내었고, 특히 그루는 가장 많은 양의 단백질을 함유하지만 전형적인 sticky dough의 문제점을 나타내었다. 수입산과 국내산의 분류에 관계없이 단백질 함량이 많은 그루, 은파, 및 탑동 등의 한국 밀들은 DNS와 같이 acid insoluble protein의 함량이 상대적으로 많으며 water soluble과 salt soluble 단백질의 함량은 상대적으로 적었다. 특히 수입 DNS 밀은 전체 단백질의 함량에 대한 40% 이상이 acid insoluble protein이며 SDS insoluble protein 함량 또한 가장 많은 양 함유되어 있었다. 반면 한국산 소맥은 대부분 연질맥인 WW와 같이 SDS insoluble protein의 함량이 상대적으로 적었다. 또한 품종이나 제품성에 관계없이 밀가루의 단백질 함량이 높을 수록 단백질의 S-S와 SH 함량이 증가하였으며, 이러한 결과는 외국문헌에서 알려진 밀가루의 특성과 특별한 차이가 없었다. 그러나 탑동밀을 제외한 4종의 한국 밀에서 gluten의 특징적인 116 kD 이상의 high molecular weight protein subunit이 관찰되었으며, 이러한 subunit은 high molecular weight glutenin subunit에 해당되는 것으로 외국문헌의 경우 subunit 2.2로 분류하기도 한다. 또한 116 kD영역에 나타나는 triplet band는 탑동을 제외한 4 종의 우리밀은 doublet로 나타나므로 단백질의 high molecular weight subunit region에서 경질맥인 탑동밀의 구조가 가장 수입밀과 일치하는 것을 관찰할 수 있었다.