• Journal of Plant Biotechnology
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• v.44 no.4
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• pp.388-393
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• 2017

We report an Agrobacterium-mediated transformation procedure optimized for 'Kyoho' that is a major table grapevine cultivar in Korea, and its transgenic plants with antifreeze protein gene of carrot (DcAFP). The full length of DcAFP coding region in accordance with the previous report was isolated from young leaves of carrot and recombined into a plant transformation vector. Ethylene inhibitors such as silver nitrate and aminoethoxyvinylglycin (AVG) supplemented in a co-cultivation medium distinctly increased frequency of shoot regeneration when explants were sub-cultured in a selection medium: particularly ten-fold higher in treatment with 0.1 mg/L AVG than one without ethylene inhibitor. Among various antibiotics and their concentrations, the combination of 150 mg/L cefotaxime plus 150 mg/L $Clavamox^{TM}$ was selected for elimination of Agrobacterium cells in addition to minimization of adverse effect on shoot regeneration, while 50 mg/L kanamycin monosulfate effectively suppressed regeneration of non-transgenic shoots. Applying the elucidated culture condition, we finally obtained a total of 5 transgenic 'Kyoho' plantlets with DcAFP, of which integration with the grapevine genome and transcription was confirmed by nucleic acid analyses.

• The KIPS Transactions:PartD
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• v.9D no.2
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• pp.297-306
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• 2002

This paper handles the design and implementation of the system for transforming the XML document bated on XML Schema being different in syntax but similar in logic, with using structured differences analysis. In the system, the merge data is generated from the source and destination documents by utilizing data registry and structured differences analysis, and then XML document is generated from the generated merge data. The XML document transformation system is designed that transformation process to the present application system from the different application system gains advantage in the aspect of time, cost, and reliability. The implementation environment of the system is that it is run on IBM compatible PC and it is developed using the software of visual basic 6.0 with the Platform of Windows 2000.

• Journal of The Korean Society of Grassland and Forage Science
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• v.26 no.4
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• pp.193-198
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• 2006

Effects of acetosyringone and on Agrobacterium-mediated transformation of orchardgrass were investigated. Embryogenic calli induced from 3 varieties, Frontier, Potomac and Roughrider, were infected and co-cultured with Agrobacterium EHA101 carrying standard binary vector pIG121Hm encoding the hygromycin phosphotransferase(HPT), neomycin phosphotransferase II(NPTII) and intron-containing ${\beta}-glucuronidase$ (intron-GUS) genes in the T-DNA region. The effects of varieties and acetosyringone(AS) concentrations on transformation and the expression of the GUS gene were investigated. Inclusion of $200{\mu}M$ AS in inoculation and co-cultivation media lead to a significant increase in stable transformation efficiency. Hygromycin resistant calli were developed into complete plants via somatic embryogenesis. GUS histochemical assay and PCR analysis of transgenic plants demonstrated that transgenes were integrated into the genome of orchardgrass.

• Journal of Korean Society on Water Environment
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• v.40 no.3
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• pp.131-139
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• 2024

This study investigated the chemical properties of Organic Soil Amendments (OSAs) made from organic waste. It also assessed the effectiveness of using these OSAs in the soil layer of Green Infrastructure (GI) to reduce stormwater runoff and non-point source pollutants. The goal was to improve the national environmental value through resource recycling and contribute to the circular economy transformation and carbon neutrality of urban GI. The OSAs used in this study consisted of spent coffee grounds and food waste compost. They were found to be nutrient-rich and stable as artificial soils, indicating their potential use in the soil layer of GI facilities. Applying OSAs to bio-retention cells and permeable pavement resulted in a reduction of approximately 11-17% in stormwater runoff and a decrease of about 16-18% in Total Phosphorus (TP) discharge in the target area. Increasing the proportion of food waste compost in the OSAs had a positive impact on reducing stormwater runoff and pollutant emissions. This study highlights the importance of utilizing recycled resources and can serve as a foundation for future research, such as establishing parameters for assessing the effectiveness of GI facilities through experiments. To enable more accurate analysis, it is recommended to conduct studies that consider both the chemical and biological aspects of substance transfer in OSAs.

• The Journal of Korean Philosophical History
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• no.59
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• pp.395-423
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• 2018

Since the middle of the 19th century, East Asia, especially Korea, was oppressed externally by the imperialism of Japan and others while internally long-lasting political convention like in-law government was driving the country into troubles at home and abroad. Witnessing such a situation and building up scholarly capability through reading over nearly all spheres, a Confucian philosopher, Ch'oe Han-gi (崔漢綺, 1803-1877), philosophized about the clue to the solution of such a choking phenomenon. Ch'oe believed that there was movement, ki (matter, material force, energy) of revolving transformation inherent in all things, people, or objects. Grasping even the principle and order of existence inherent in things from the viewpoint of ki, he tried to change the traditional theory on the law of existence of things that there existed an ontological reason lacking in motility, which was more dominant than anything existing in the universe and which ruled over the world. From this, he elucidated that the Way, an identity that rules over all affairs and things, was not simply one but diverse. This means, on one hand, that he tentatively accepted the relativity of things. On the other hand, he also taught us "oneness in all things" in which all including human beings exist in close connection to each other transcending the relativity or "agreement" in which they exist in harmony as one and "unity" in which they are united into one.

• Korean Journal of Materials Research
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• v.11 no.12
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• pp.1057-1062
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• 2001

We investigated the change of mechanical properties for TiNi shape memory alloy by heat treatment. 6061Al matrix composites with TiNi shape memory alloy as reinforcement were fabricated by vacuum casting. TiNi alloy has the maximum tensile strength at 673K treated and there is no change of tensile strength and hardness at 448K treated. The composites, prepared by vacuum casting, showed good interface bonding by vacuum casting. It was about 3$\mu\textrm{m}$ of thickness of the diffusion layer. Tensile strength of the composite was in higher than that of 6061Al alloy as increased value of about 70MPa at room temperature and about 110MPa at 363K. We thought that the increase of the tensile strength at 363K was due to reverse transformation of the TiNi shape memory alloy.

• The Journal of Internal Korean Medicine
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• v.12 no.2
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• pp.138-147
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• 1991

Review of literature on stomachache. According to investigated the pathology of a stomachache about all sorts of falling ill, I have been obtained following conclusions. 1. The cause of Ui Wan Tong are clod-wet-ui wan, sik juk, dam eum, a huy1 be made of gi bul soon (氣不順). 2. Pain of Je sang bu are called jung wan tong, dae bok tong, jung cho tong, cause are sik juk, han sa, chung juk. pain of je ha bu are called so bok tong, hache tong, soh bok tong, so bok are devided two part, one is so eum sin the other is gul eum gan, cause are jo gyul dae jang, yul gyu1 bang kwang, hyul gyul bang kwang, porak hu ect. 3. Pain of je bu are called je bok tong, hwan je e tong, so bok je ju tong, and cause are juk yu1, jo si, dam hwa. Pain of yang hyup bu are called yang hyup ha so bok tong, yang bang ge hyup tong, yang hup jisang tong, cause are ban gi,yang myung jo geom. According to above results, the patholosy of a stomach ache about the part of falling ill in a course of transformation on the epidemic fever comes into the viscera.

• The Transactions of the Korea Information Processing Society
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• v.4 no.5
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• pp.1380-1390
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• 1997

This paper presents a systolic array design method which derives 1 or 2 dimensional optimal planar systolic arrays from a given n dimensional problem represented as a regular recurrence equation and its implementation called a systolic array design system(SADS).The SADS parses a regular recurrence equation and gets the information such as problem space, data dependence vectors. and intial data positions. Systolic arrays are automati-cally derived by the space-time transformation form the information to be abeaired in the parsing phase.The SADS allows us to verify the parallel execution of the derived systolic aooay through the graghical interface.

• Journal of the Computational Structural Engineering Institute of Korea
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• v.20 no.1
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• pp.65-73
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• 2007

In the inverse perturbation method, enormous computational resource was required to obtain reliable results, because all unspecified DOFs were considered as unknown variables. Thus, in the present study, a reduced system method is used to condense the unspecified DOFs by using the specified DOFs, and to improve the computational efficiency as well as the solution accuracy. In most of the conventional reduction methods, transformation errors occur in the transformation matrix between the unspecified DOFs and the specified DOFs. Thus it is hard to obtain reliable and accurate solution of inverse perturbation problems by reduction methods due to the error in the transformation matrix. This numerical trouble is resolved in the present study by adopting iterative improved reduced system(IIRS) as well as by updating the transformation matrix at every step. In this reduction method, system accuracy is related to the selection of the primary DOFs and Iteration time. And both are dependent to each other So, the two level condensation method (TLCS) is selected as Selection method of primary DOFs for increasing accuracy and reducing iteration time. Finally, numerical verification results of the present iterative inverse perturbation method (IIPM) are presented.

• Journal of Cadastre & Land InformatiX
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• v.46 no.2
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• pp.183-196
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• 2016

Ground control points are needed for precision geometric correction of satellite images, and the coordinates of a high-quality ground control point can be obtained from the GPS measurement. However, considering the GPS measurement requires an excessive amount o f t ime a nd e fforts, there is a need for coming up with an alternative solution to replace it. Therefore, we examined the possibility of replacing the existing GPS measurement with coordinates available at online maps to acquire the coordinates of ground control points. To this end, we examined error amounts between the coordinates of ground control points obtained through Daum Map API, and them compared the accuracies between three types of coordinate transformation equations which were used for geometric correction of satellite images. In addition, we used the coordinate transformation equation with the highest accuracy, the coordinates of ground control point obtained through the GPS measurement and those acquired through D aum M ap A PI, and conducted geometric correction on them to compare their accuracy and evaluate their effectiveness. According to the results, the 3rd order polynomial transformation equation showed the highest accuracy among three types of coordinates transformation equations. In the case of using mid-resolution satellite images such as those taken by Landsat-8, it seems that it is possible to use geometrically corrected images that have been obtained after acquiring the coordinates of ground control points through Daum Map API.