• 한국어병학회지
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• 제30권2호
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• pp.71-78
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• 2017

The glycoprotein of novirhabdoviruses is known to play a critical role in the determination of host specificity. Viral hemorrhagic septicemia viruses (VHSVs) in different genotypes have different glycoprotein sequences and show different preferences for specific cell lines. In this study, to know whether the glycoprotein is solely responsible for the host cell preference of VHSV, a recombinant VHSV expressing vesicular stomatitis virus (VSV) glycoprotein instead of VHSV IVa glycoprotein (rVHSV-VSV-G) was generated by reverse genetics and inoculated into several fish cell lines, then, cytopathic effect (CPE) and viral growth caused by rVHSV-VSV-G infection were compared with those caused by rVHSV-wild that was previously generated and has the same genomic sequence with wild-type VHSV except a few nucleotides. The plaque numbers of rVHSV-VSV-G were significantly higher in EPC, BF-2 and GF cells than those of rVHSV-wild. However, in HINAE cells (originated from olive flounder), rVHSV-VSV-G titer was significantly lower than rVHSV-wild titer, and both recombinant VHSVs were not grown well in CHSE-214 cells. Although statistical significances were detected in the titers between rVHSV-wild and rVHSV-VSV-G in several cell lines, the cell line-preference order of rVHSV-VSV-G was not different from that of rVHSV-wild. These results suggest that the replacement of VHSV glycoprotein may not completely change host cell preference, and other regions of VHSV might also involve in the determination of host cell preference.

• 동의생리병리학회지
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• 제25권1호
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• pp.29-36
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• 2011

Nardostachyts chinensis (N. chinensis) belonging to the family Valerianaceae has been used to elicit stomachic and sedative effects. The MAPKs are serine/threonine kinases involved in the regulation of various cellular responses, such as cell proliferation, differentiation and apoptosis. The PKC also plays a key role in regulating the response of hematopoietic cells to both physiological and pathological inducers of proliferation and differentiation. This study investigated the signaling pathways on the U937 cell differentiation induced by N. chinensis. N. chinensis induced the differentiation of U937 cells, as shown by increased of differentiation surface antigen CD11b. Activation of ERK increased time-dependently in differentiation of U937 cells induced by N. chinensis, but activations of JNK and p38 were unaffected. Inhibitor of ERK (PD98059) significantly reduced CD11b expression induced by N. chinensis in U937 cells. In addition, N. chinensis increased protein level of PKC ${\beta}$I and PKC ${\beta}$II isoforms, but the protein level of PKC ${\alpha}$ and PKC ${\gamma}$was constant. PKC inhibitors (GF 109203X and H-7) inhibited U937 cell differentiation and the ERK activation induced by N. chinensis. These results indicated that PKC and ERK may be involved in U937 cell differentiation induced by N. chinensis.

• Journal of Plant Biotechnology
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• 제3권2호
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• pp.107-112
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• 2001

The kinetics of Ti-transformed Salvia miltiorrhiza cell cultures was studied in 250-$m\ell$ shake flasks by using B5 medium with addition of 30 gfL of sucrose. In the cell cultures, the maximum cell mass obtained was 11.5 g DW/L on day 15. The highest amount of phenolic compounds - rosmarinic acid (RA) and lithospermic acid B (LAB) reached 871.3 mg/L (day 15) and 121.3 mg/L (day 13), respectively. The total tanshinone production, i.e., intracellular plus extracellular cryptotanshinone, tanshinone 1, and tanshinone IIA, was 5.3 mg/L on day 13. For the cultivations in 2.4-L stirred bioreactors, the residual sugar level and medium conductivity were a little higher in a small turbine impeller reactor ($T_s$) than those in a large turbine impeller reactor ($T_L$), while a higher cell density was obtained in the $T_L$. For the production of tanshinones and phenolics, better results were obtained in the $T_L$ than in the $T_s$. In the $T_L$, similar or even a little higher production titers of tanshinones and phenolic compounds were achieved compared to those in the flasks. The results suggest that the shake flask results could be successfully scaled up to the $T_L$ reactor. Such a large impeller reactor like $T_L$ may be better than a small impeller one for the large-scale production of the valuable metabolites by the suspension cultures of Ti transformed S.miltiorrhiza cells. This is considered due to the beneficial culture environment in the $T_L$, such as low shear rates as estimated theoretically.

• KSBB Journal
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• 제17권2호
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• pp.203-206
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• 2002

신체성장은 세포수가 증가하고 세포의 크기가 커지면서 신체의 크기가 증가하는 것으로서 몸을 구성하는 성분인 단백질이 증가를 수반하고 골격과 근육이 커지며 이득의 기능도 강화된다. 사람의 키가 커진다는 것은 팔과 다리의 긴 뼈가 늘어나야 하는 것인데 이와같은 성장은 호르몬의 작용에 의해서 이루어진다. 본 연구에서는 이와같은 성장에 미치는 영향을 검토하기 위해 성장호르몬 및 IGF-1의 분비촉진 여부를 확인하였다. 체외 래트 성장호르몬의 분비량은 88 - 99 ng/mL으로 대조군에 비해 약 6배 이상의 성장호르몬이 분비되었다. 또한 생체내 IGF-1 분비촉진 효과실헐에서는 YGF 투여 후 8시간에서 1140 ng/mL 정도로 대조군에 비해 현저하게 높은 혈중 IGF-1의 농도가 유지되었다. YGF를 장기 복용시킨 경우 실제로 대퇴부 배의 길이성장이 약 6% 정도 이루어졌음을 확인할 수 있었다. 따라서 본 실험을 통해 확인된 성장호르몬 및 IGF-1 분비촉진을 유발하는 식물 추출물 YGF에 대해 좀더 연구개발하여 키 성장이 저조한 어린이 및 청소년에게 신체성장에 도움이 될 수 있도록 되었으면 한다.

• 한국생활과학회지
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• 제15권3호
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• pp.481-490
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• 2006

Adjusting pressure level in the construction of athletes' tight-fitting garments by reducing the elastic knit pattern is a challenging subject, which influences the performance of the wearer directly. Therefore, in this study, relationship between the reduction rates of the basic pattern obtained from 3D human scan data and resultant clothing pressure was explored to improve the fit and pressure exerted by clothing. 3D scan data were obtained using Cyberware and they were transformed into a flat pattern using software based on Runge-Kutta method. Reduction rate was examined by subjective wear test as well as objective pressure measurement. As a result, difference in the length between the original 3D body scan data and the 2D tight-fitting pattern was 0.02$\sim$0.50cm (0.05$\sim$1.06%), which was within the range of tolerable limits in making clothes. Among the five garments, the 3T-pattern was superior in terms of subjective sensation and fit. The pressure of the 3T pattern was 2$\sim$4 gf/cm2 at five locations on the body, which is almost the same or a bit higher than that of Z-pattern. In the case of tight-fitting overall garment, the reduction rate of the pattern in the wale direction is more critical to the subjective sensation than the course direction. It is recommended that the reduction grading rules of course direction should be larger than that of Ziegert for a better fit of tight-fitting garments. In the case of wale direction, however, reduction grading rule should be kept the same as suggested earlier by Ziegert (1988).

• 한국생활과학회지
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• 제24권2호
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• pp.255-266
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• 2015

In this study, we have developed the ergonomic pattern from the 3D human body reflecting cycling posture and extensibility of the stretch fabrics. Adjusting pressure level in the construction of athlete's tight-fitting stretch garments by reducing the original pattern is a challenging subject, which influence on the performance of the wearer directly. Therefore, in this study, relationships between the reduction rates of the 2D pattern obtained from the 3D human scan and resultant clothing pressure were explored to improve the fit and pressure exerted by reduced clothing pattern. Subjective wear sensations of the experimental garments were rated using a seven-point Likert scale on two consecutive days. While wearing the garments, subjects were asked to take five different postures including waist flexion, sitting and others. A Likert-type scale was used for the evaluation, with 7 points indicating the best fit in tight-fitting pants. Comparing 2/3T-pattern with T-pattern, the latter was superior to 2/3T-pattern in terms of adhere well to the waist and hip area in the 0.032 significance level. T-pattern was superior to 2/3T-pattern in terms of fitting and wear comfort. As results, the pattern obtained from the flexed body reflecting cycling posture already included the contraction and extension of the skin while cycling posture, so that the extra ease for movement and good fit was not need to be considered. The optimized reduction rates were determined with the proposed reduction rate, the resultant pressure range was within the range of $0.5{\sim}3.0gf/cm^2$ at eight locations on the body except front waist band and thigh band.

• 한국정보통신학회논문지
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• 제9권3호
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• pp.684-689
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• 2005

본 논문에서는 수평$\cdot$수직 에지의 형태학적 정보를 이용한 차량 번호판 추출과 개선된 신경망을 이용한 차량 번호판 인식 시스템을 제안한다. 번호판 영역은 수평 수직에지의 형태학적 정보를 이용하여 추출하고 개별 문자는 히스토그램 방법과 위치 정보를 이용한 방법에 윤곽선 추적 알고리즘을 병합하여 추출한다. 개별 문자 인식은 ART-1 알고리즘을 지도 학습 방법과 결합한 개선된 신경망을 제안하여 차량 번호판 인식에 적용한다. 제안된 방법의 성능을 확인하기 위하여 실제 차량 번호판들을 대상으로 실험한 결과, 수평$GF(2^m)$수직 에지의 형태학적 정보를 이용한 차량 번호판 추출 방법이 임계화를 이용한 차량 번호판 추출 방법, RGB와 HSI 컬러 정보를 각각 이용한 차량 번호판 추출 방법보다 추출룰이 개선되었으며, 인식 성능도 개선된 신경망의 학습 알고리즘이 기존의 학습 알고리즘들 보다 우수한 성능이 있음을 확인하였다.

• Biomolecules & Therapeutics
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• 제21권1호
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• pp.42-48
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• 2013

Nystatin, a polyene antifungal antibiotic, is a cholesterol sequestering agent. The antifungal agent alters composition of the plasma membrane of eukaryotic cells, whereas its effects on cells are poorly investigated. In the current study, we investigated the question of whether nystatin was able to induce expression of macrophage inflammatory protein-1 (MIP-1). THP-1 cells rarely express MIP-$1{\alpha}$ and MIP-$1{\beta}$, however, upon exposure to nystatin, significantly elevated expression of MIP-$1{\alpha}$ and MIP-$1{\beta}$ was observed in a dose-dependent fashion at the messenger and protein levels. Cellular factors activated by nystatin as well as involved in nystatin-induced expression of MIP-1 proteins were identified in order to understand the molecular mechanisms of action of the anti-fungal agent. Treatment with nystatin resulted in enhanced phosphorylation of Akt, ERK, p38 MAPK, and JNK. Abrogation or significant attenuation of nystatin-induced expression of MIP-$1{\alpha}$ and MIP-$1{\beta}$ was observed by treatment with Akt inhibitor IV, LY294002, and SP6001250. Inhibition of ERK or p38MAPK using U0126 and SB202190 did not lead to attenuation of MIP-1 expression. In addition, inhibitors of protein kinase C, such as GF109203X and Ro-318220, also attenuated expression of MIP-1. These results indicate that nystatin is able to activate multiple cellular kinases and, among them, Akt and JNK play primary roles in nystatin-induced expression of MIP-1 proteins.

• 생태와환경
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• 제34권2호통권94호
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• pp.133-139
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• 2001

이 연구는 식물플랑크톤(Scenedesmus sp.)이 영양염류를 흡수하는 원리를 이용하여, 영양염류를 제거하기 위하여 실시하였다. 식물플랑크톤을 교반하여 배양할 경우, 가장 빨리 증식되었고, 오수방류수를 식물플랑크톤 배양액에 50% 되도록 혼합하여, 약 2일 동안 배양시키는 것이 영양염류를 제거하는데 효율적이었다. 이러한 최적조건에서 증식된 식물플랑크톤을 GF/C glass fiber filter로 여과하여 분석한 결과, TKN는 88% 이상 제거되었고, DIP는 70${\sim}$80% 제거되었으며, 이때 식물플랑크톤이 최대한 1300mg/m$^{3}$로 증식되었다. 또한, 3시간 간격으로 영양염류의 이용과정을 분석한 결과, 식물플랑크톤의 증식은 NH$_{3}$-N(r$^{2}$=0.96)와 DIP(r$^{2}$=0.92)와는 높은 상관관계를 보였다. 영양염류를 섭취하여 증식된 식물플랑크톤을 여과시키거나, 동물플랑크톤에 적용시켜 먹이연쇄 관계를 이용한다면, 생태공학적으로 영양염류를 제거할 수 있고, 수계의 부영양화를 방지할 수 있을 것으로 사료된다.

• 한국진공학회:학술대회논문집
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• 한국진공학회 2011년도 제40회 동계학술대회 초록집
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• pp.264-264
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• 2011

Poly [(N, N'-oxydiphenylene) pyromellitimide], polyimide (PI) film은 기계적 강도가 매우 우수하고 열적, 화학적 안정성이 뛰어난 재료로서 전자제품의 소형화, 경령화, 고성능화를 위한 차세대 flexible electronic device에 적용하기 위하여 많은 연구가 진행되고 있다. 그러나 PI의 특성상, 매우 낮은 표면에너지로 인해 금속과의 접촉력이 좋지 않은 단점을 가지고 있다. 본 연구에서는, 금속박막과 PI film 과의 접촉력을 증가시키기 위해 remote-type modified dielectric barrier discharge (DBD) module을 이용하여 대기압 플라즈마 표면처리를 하였다. 실험에 사용된 gas composition은 각각 $N_2$/ He/ $SF_6$, $N_2$/ He/ $O_2$, $N_2$/ He/ $SF_6$/ $O_2$, $N_2$/ He/ $SF_6$/ $O_2$ 이다. $N_2$/ He/ $SF_6$/ $O_2$ gas composition을 이용하여 PI 표면을 플라즈마 처리한 경우, C=O 결합이 PI film 위에 생성됨으로써, 접촉각이 매우 낮게 형성됨을 관찰할 수 있었다. 이와는 반대로 $N_2$/ He/ $SF_6$ gas composition 을 사용하였을 경우에는 C-Fx 화학적 결합이 생성되기 때문에 가장 높은 접촉각이 형성됨을 관찰할 수 있었다. 특히, $N_2$ (40 slm)/ He (1 slm)/ $SF_6$ (1.2 slm) gas composition에 $O_2$ gas를 0.2 slm부터 1.0 slm까지 변화시켜가며 PI film 표면을 처리한 결과, $O_2$ gas를 0.9 slm 첨가하였을 때, 가장 낮은 $9.3^{\circ}$의 접촉각을 얻을 수 있었다. 이는 0.9 slm의 $O_2$ gas를 첨가하였을 때, 가장 많은 양의 $O_2$ radical이 생성되기 때문에 많은 양의 C=O 결합이 생성되기 때문이다. 최적화된 $N_2$ (40 slm)/ He (1 slm)/ $SF_6$ (1.2 slm)/ $O_2$ (0.9 slm) gas composition 조건에서 Ag film과 PI film과의 접촉력을 관찰할 결과, 111 gf/mm를 얻을 수 있었다.