• Animal Systematics, Evolution and Diversity
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• 제19권2호
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• pp.297-304
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• 2003

Mitochondrial cytochrome b 유전자를 이용하여 한국에서 채집된 5종을 포함한 올빼미과(Strigidae)의 12속 31종의 계통분석을 하였다. Maximum likelihood 분석과, Kimura two-parameter와 p-distance를 이용하여 유전적 근연 관계를 측정하였다. 본 연구에 이용된 959개의 염기서열 가운데, 459개의 서열에서 변이를 확인하였고, 398개의 서열은 계통학적 정보를 가졌다. 올빼미과는 Clade I (Aegolius) , Clade II (Athene, Micrathene, Glaucidium, Surnia), 그리고 Clade III(Bubo, Nyctea, Pulsatrix, Strix, Otus, Ptilopsis, Ninox) 등 세 개의 그룹으로 나뉘어졌다. 또한 Otus속은 지리적으로 다른 두 개의 그룹으로 분리되는 것이 확인되었다.

• 한국동물학회:학술대회논문집
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• 한국동물학회 2001년도 한국생물과학협회 학술발표대회
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• pp.126.1-126
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• 2001

No Abstract, See Full Text

• Journal of Microbiology and Biotechnology
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• 제29권12호
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• pp.1957-1968
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• 2019

Tetraselmis is a green algal genus, some of whose species are important in aquaculture as well as biotechnology. In algal culture, fluorescent lamps, traditional light source for culturing algae, are now being replaced by a cost-effective light-emitting diodes (LEDs). In this study, we investigated the effect of LED light of different wavelengths (white, red, yellow, and blue) on the growth of Tetraselmis suecica and its associated microbial community structures using the next-generation sequencing (NGS). The fastest growth rate of T. suecica was shown in the red light, whereas the slowest was in yellow. The highest OTUs (3426) were identified on day 0, whereas the lowest ones (308) were found on day 15 under red light. The top 100 OTUs associated with day 0 and day 5 cultures of T. suecica under the red and yellow LED were compared. Only 26 OTUs were commonly identified among four samples. The highest numbers of unique OTUs were identified at day 0, indicating the high degree of initial microbial diversity of the T. suecica inoculum. The red light-unique OTUs occupied 34.98%, whereas the yellow-specific OTUs accounted for only 2.2%. This result suggested a higher degree of interaction in T. suecica culture under the red light, where stronger photosynthesis occurs. Apparently, the microbial community associated with T. suecica related to the oxygen produced by algal photosynthesis. This result may expand our knowledge about the algae-bacteria consortia, which would be useful for various biotechnological applications including wastewater treatment, bioremediation, and sustainable aquaculture.

• 한국토양비료학회지
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• 제45권6호
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• pp.1100-1107
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• 2012

화학비료의 장기 시용이 벼 내생 세균 군집에 미치는 영향을 조사하기 위해서 국립농업과학원의 장기 비료 연용 포장에서 재배한 벼 뿌리의 내생균의 군집을 파이로시퀀싱 기법으로 분석하였다. 3요소구 (APK)와 무비구 (NF) 시료에서 직접 DNA를 추출하여 세균에 특이적인 barcode PCR을 수행한 후 454 파이로시퀀싱을 하였다. 두 시료 (3요소구, 무비구)에서 1,900개의 염기서열을 얻었으며, 각각 177개와 72개의 OTU로 분류하였다. 두 시료는 22개의 OTU를 공유하였으며, 이들 OTU는 두 시료에서 모두 우점하였다. 특히 Pseudomonas속에 속하는 OTU의 비율이 매우 높았다. 문 (phylum) 수준에서 우점하는 내생균은 두 시료 모두 Gammaproteobacteria, Alphaproteobacteria, Betaproteobacteria, Actinobacteria 등 이었다. 처리구별로 계산한 다양성 지수는 3요소구 시료에서 더 높았다. 본 연구를 통해 장기간 비료 시용은 식물체내 존재하는 내생균 군집 구조에 영향을 주며, 벼 뿌리의 내생 세균의 군집 다양성을 증가시키는 것을 알 수 있었다.

• Journal of Microbiology and Biotechnology
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• 제25권8호
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• pp.1195-1204
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• 2015

Increasing evidence suggests that gut microbiota underpin the development of health and longevity. However, our understanding of what influences the composition of this community of the longevous has not been adequately described. Therefore, illumina sequencing analysis was performed on the gut microbiota of centenarians (aged 100-108 years; RC) and younger elderlies (aged 85-99 years; RE) living in Bama County, Guangxi, China and the elderlies (aged 80-92 years; CE) living in Nanning City, Guangxi, China. In addition, their diet was monitored using a semiquantitative dietary questionary (FFQ 23). The results revealed the abundance of Roseburia and Escherichia was significantly greater, whereas that of Lactobacillus, Faecalibacterium, Parabacteroides, Butyricimonas, Coprococcus, Megamonas, Mitsuokella, Sutterella, and Akkermansia was significantly less in centenarians at the genus level. Both clustering analysis and UniFraq distance analysis showed structural segregation with age and diet among the three populations. Using partial least square discriminate analysis and redundancy analysis, we identified 33 and 34 operational taxonomic units (OTUs) as key OTUs that were significantly associated with age and diet, respectively. Age-related OTUs were characterized as Ruminococcaceae, Clostridiaceae, and Lachnospiraceae, and the former two were increased in the centenarians; diet-related OTUs were classified as Bacteroidales, Lachnospiraceae, and Ruminococcaceae. The former two were deceased, whereas the later one was increased, in the high-fiber diet. The age and high-fiber diet were concomitant with changes in the gut microbiota of centenarians, suggesting that age and high-fiber diet can establish a new structurally balanced architecture of gut microbiota that may benefit the health of centenarians.

• Animal Systematics, Evolution and Diversity
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• 제11권2호
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• pp.183-197
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• 1995

한국산 Phintella속에 속하는 6종의 기재와 동정은 불충분하고 불확실한 상태에 있다. 본 논문에서는 한국미기록종인 P.popovi를 포함한 7종에 대하여 그림, 검색표와 함께 재기재를 하였고, Wesolowska(198la)에 의하여 한국산으로 보고된 바 있는 Icius munitus를 P. cavaleriei로 synonym처리를 하였다. 또, 본인의 동정과 찍짓기를 검정하기 위하여, 134개체에 대한 표준편차 0.05 이하의 13개 형질비값으로 다변량분석을 실시하였다. 134개체에 대한 13개의 형질비값으로 시행된 판별분석의 결과는 만족스럽지 못하였으나, 13개의 형질비값에 대한 14개 OTU의 평균비값으로 시행된 군집분석의 결과는 다른 쌍들에 비하여 비유성이 큰 P. abnormis를 제외하고 저자의 짝짖기 결과와 일치함을 보여주었다. 완벽한 결과를 보여주지는 못하였으나. 단 한 종만 찍을 만들지 못하였기 때문에 전체 7종의 쌍을 얻을 수는 있었다. 이 방법이 보다 개선되면, 옳바른 짝을 검정하거나 동정할 때 유용하게 사용될 수 있을 것으로 생각된다.

• 한국미생물·생명공학회지
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• 제44권4호
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• pp.550-556
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• 2016

지하수의 열을 이용한 히트펌프 시스템에서는 열 효율 유지를 위한 클로깅 현상이 고려되어야 한다. 클로깅 현상은 토양 지하수 환경에서 이화학적 요인 외에도 미생물학적 요인으로 발생한다. 이번 연구에서는 안정적인 지하수 열원 냉난방 시스템 운영을 위하여, 대수층 수위강하의 영향을 받지 않는 지하 10 미터 지점에서 불교란 시추 슬라임의 초기상태 원핵생물 다양성을 조사하였다. 세균은 문 수준에서 Proteobacteria (20.8%), Acidobacteria (18.8%), Chloroflexi (16.9%) 및 Firmicutes (10.2%) 등으로 나타났고, 속 수준에서는 Koribacter속 등 144개속이 분석되었다. 고세균은 문 수준에서 Thaumarchaeota (42.8%), Crenarchaeota (36.9%) 및 Euryarchaeota (17.4%)이 나타났으며, 강 수준에서 약 69.4% 비율로 Miscellaneous Crenarchaeota Group (MCG), Finnish Forest Soil Type B (FFSB) 및 Thermoplasmata가 분석되었다. Operational taxonomic units (OTUs)는 세균 3,565 및 고세균 836 OTUs로 나타났고, 세균이 고세균에 비해 풍부하며 우점도가 낮게 나타났다. 또한, 관정 막힘 현상을 유발할 가능성 있는 세균 후보군 135개(1.9%) reads가 분석되었으며, 향후 클로깅 현상에 대한 연구에 자료로 활용할 수 있을 것으로 기대된다.

• Animal Bioscience
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• 제34권12호
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• pp.1921-1929
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• 2021

Objective: The intestinal microbiota enhances nutrient absorption in the host and thus promotes heath. Qinghai semi-fine wool sheep is an important livestock raised in the Qinghai-Tibetan Plateau; however, little is known about the bacterial microbiota of its intestinal tract. The aim of this study was to detect the microbial characterization in the intestinal tract of the Qinghai semi-fine wool sheep. Methods: The bacterial profiles of the six different intestinal segments (duodenum, jejunum, ileum, cecum, colon and rectum) of Qinghai semi-fine wool sheep were studied using 16S rRNA V3-V4 hypervariable amplicon sequencing. Results: A total of 2,623,323 effective sequences were obtained, and 441 OTUs shared all six intestinal segments. The bacterial diversity was significantly different among the different intestinal segments, and the large intestine exhibited higher bacterial diversity than the small intestine. Firmicutes, Bacteroidetes, and Patescibacteria were the dominant phyla in these bacterial communities. Additionally, at the genus level, Prevotella_1, Candidatus_Saccharimonas, and Ruminococcaceae_UCG-005 were the most predominant genus in duodenal segment, jejunal and ileal segments, and cecal, colonic, and rectal segments, respectively. We predicted that the microbial functions and the relative abundance of the genes involved in carbohydrate metabolism were overrepresented in the intestinal segments of Qinghai semi-fine wool sheep. Conclusion: The bacterial communities and functions differed among different intestinal segments. Our study is the first to provide insights into the composition and biological functions of the intestinal microbiota of Qinghai semi-fine wool sheep. Our results also provide useful information for the nutritional regulation and production development in Qinghai semi-fine wool sheep.

• 한국축산식품학회지
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• 제41권4호
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• pp.701-714
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• 2021

In this study, the microbial community succession and the protein hydrolysis of donkey meat during refrigerated (4℃) storage were investigated. 16S rDNA sequencing method was used to analyze the bacteria community structure and succession in the level of genome. Meanwhile, the volatile base nitrogen (TVB-N) was measured to evaluate the degradation level of protein. After sorting out the sequencing results, 1,274,604 clean data were obtained, which were clustered into 2,064 into operational taxonomic units (OTUs), annotated to 32 phyla and 527 genus. With the prolonging of storage time, the composition of microorganism changed greatly. At the same time, the diversity and richness of microorganism decreased and then increased. During the whole storage period, Proteobacteria was the dominant phyla, and the Photobacterium, Pseudompnas, and Acinetobacter were the dominant genus. According to correlation analysis, it was found that the abundance of these dominant bacteria was significantly positively correlated with the variation of TVB-N. And Pseudomonas might play an important role in the production of TVB-N during refrigerated storage of donkey meat. The predicted metabolic pathways, based on PICRUSt analysis, indicated that amino metabolism in refrigerated donkey meat was the main metabolic pathways. This study provides insight into the process involved in refrigerated donkey meat spoilage, which provides a foundation for the development of antibacterial preservative for donkey meat.

• Journal of Ginseng Research
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• 제46권1호
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• pp.91-103
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• 2022

Background: Red ginseng extract boosts immunity against inflammation and cancer in the human body. However, studies on the effects of red ginseng extract on the gut microbiome remain unexplored. Methods: In 2019, the positive effects and changes in the gut microbiome after administering 1 pack (3 g) of red ginseng extract per day to 53 adults aged 40 to 75 for 24 weeks were investigated. The gut microbial environment changes were qualitatively and quantitatively analyzed using next-generation sequencing and real-time polymerase chain reaction technology. Results: On comparing and analyzing alpha diversity and beta diversity, the microbial pattern showed significant differences (OTUs p = 0.003, chao1 p < 0.001, Bray-Curtis p = 0.001) before and after ingestion of red ginseng extract, indicating that gut microbial richness increased after ingestion. Moreover, after comparing and analyzing the gut microbiome's differences after red ginseng extract intake, significant differences were noted between three strains at the phylum level and among 57 strains at the genus level. Conclusion: This study proposes the potential use of red ginseng extract as a prebiotic after confirming its positive effects, including increasing gut microbiome richness, reducing harm to the gut microbiome, and increasing the number of some strains in the gut microbiome.