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http://dx.doi.org/10.4014/mbl.1606.06006

Genetic Prokaryotic Diversity in Boring Slime from the Development of a Groundwater Heat Pump System  

Kim, Heejung (School of Earth and Environmental Sciences, Seoul National University)
Lee, Siwon (Water Supply & Sewerage Research Division, National Institute of Environmental Research)
Park, Junghee (School of Earth and Environmental Sciences, Seoul National University)
Joun, Won-Tak (School of Earth and Environmental Sciences, Seoul National University)
Kim, Jaeyeon (School of Earth and Environmental Sciences, Seoul National University)
Kim, Honghyun (School of Earth and Environmental Sciences, Seoul National University)
Lee, Kang-Kun (School of Earth and Environmental Sciences, Seoul National University)
Publication Information
Microbiology and Biotechnology Letters / v.44, no.4, 2016 , pp. 550-556 More about this Journal
Abstract
Groundwater heat pump (GWHP) systems must consider phenomena such as clogging to improve system efficiency and maintenance. In this study, we evaluated the prokaryotic diversity in a boring slime sample obtained at a depth of 10 m, which represented an undisturbed sample not affected by aquifer drawdown. Bacteria belonging to the phyla Proteobacteria (20.8%), Acidobacteria (18.8%), Chloroflexi (16.9%), and Firmicutes (10.2%) were found. Additionally, 144 species were identified as belonging to the genus Koribacter. Archaeal phyla were detected including Thaumarchaeota (42.8%), Crenarchaeota (36.9%), and Euryarchaeota (17.4%) and the class level comprised the miscellaneous Crenarchaeota group (MCG), Finnish forest soil type B (FFSB), and Thermoplasmata, which collectively accounted for approximately 69.4% of the detected Archaea. Operational taxonomic units (OTUs) were analyzed to reveal 3,565 bacterial and 836 archaeal OTUs, with abundances of 7.81 and 6.68, and richnesses of 5.96E-4 and 2.86E-3, respectively. The distribution of the groundwater microbial community in the study area showed a higher proportion of non-classified or unidentified groups compared to typical communities in surface water and air. In addition, 135 (approx. 1.9%) reads were assigned to a bacterial candidate associated with clogging.
Keywords
Boring slime; groundwater heat pump system; Pokaryotic diversity;
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Times Cited By KSCI : 7  (Citation Analysis)
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