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http://dx.doi.org/10.4014/jmb.1910.10046

Assessment of the Dynamics of Microbial Community Associated with Tetraselmis suecica Culture under Different LED Lights Using Next-Generation Sequencing  

Yang, Su-Jeong (Interdisciplinary Program of Biomedical, Mechanical and Electrical Engineering, Pukyong National University)
Kim, Hyun-Woo (Interdisciplinary Program of Biomedical, Mechanical and Electrical Engineering, Pukyong National University)
Choi, Seok-Gwan (National Institute of Fisheries Science (NIFS))
Chung, Sangdeok (National Institute of Fisheries Science (NIFS))
Oh, Seok Jin (Department of Oceanography, Pukyong National University)
Borkar, Shweta (Department of Chemistry, Pukyong National University)
Kim, Hak Jun (Department of Chemistry, Pukyong National University)
Publication Information
Journal of Microbiology and Biotechnology / v.29, no.12, 2019 , pp. 1957-1968 More about this Journal
Abstract
Tetraselmis is a green algal genus, some of whose species are important in aquaculture as well as biotechnology. In algal culture, fluorescent lamps, traditional light source for culturing algae, are now being replaced by a cost-effective light-emitting diodes (LEDs). In this study, we investigated the effect of LED light of different wavelengths (white, red, yellow, and blue) on the growth of Tetraselmis suecica and its associated microbial community structures using the next-generation sequencing (NGS). The fastest growth rate of T. suecica was shown in the red light, whereas the slowest was in yellow. The highest OTUs (3426) were identified on day 0, whereas the lowest ones (308) were found on day 15 under red light. The top 100 OTUs associated with day 0 and day 5 cultures of T. suecica under the red and yellow LED were compared. Only 26 OTUs were commonly identified among four samples. The highest numbers of unique OTUs were identified at day 0, indicating the high degree of initial microbial diversity of the T. suecica inoculum. The red light-unique OTUs occupied 34.98%, whereas the yellow-specific OTUs accounted for only 2.2%. This result suggested a higher degree of interaction in T. suecica culture under the red light, where stronger photosynthesis occurs. Apparently, the microbial community associated with T. suecica related to the oxygen produced by algal photosynthesis. This result may expand our knowledge about the algae-bacteria consortia, which would be useful for various biotechnological applications including wastewater treatment, bioremediation, and sustainable aquaculture.
Keywords
Next-generation sequencing; metagenomics; LED; microalgae; microbial community;
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Times Cited By KSCI : 6  (Citation Analysis)
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