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Isolation and Identification of Ampicillin-resistant Bacteria in Changwon (창원근교에서의 ampicillin 내성세균의 분리 및 동정)

  • Bae, Young-Min
    • Journal of Life Science
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    • v.28 no.12
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    • pp.1529-1535
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    • 2018
  • The number of antibiotic-resistant bacteria is increasing rapidly while the discovery rate of new antibiotics is in decline. A systematic study is therefore necessary to investigate which bacteria are resistant to medically important antibiotics and how high that resistance is. To that end, this study aimed to analyze which bacteria demonstrated resistance to ampicillin, one of the currently most-widely used medical antibiotics. Water samples were collected from the Changwon-Cheon that runs through Changwon City and from the pond in front of the dormitory building at Changwon University. Hundreds of ampicillin-resistant colonies were obtained and 22 morphologically distinct examples were chosen for further study. These bacteria were identified by amplifying their 16S rRNA genes and comparing those sequences with data in GenBank. The bacteria was identified as belonging to 10 families, 12 genera, and 17 species, and all were able to grow in the presence of $50{\mu}g/ml$ ampicillin while seven showed growth at ampicillin concentrations as high as 1.5 mg/ml.

Occurrence of Toxigenic Fusarium spp. and Zearalenone in Scabby Rice Grains and Healthy Ones (붉은곰팡이 감염 벼와 건전 벼의 독성 곰팡이와 제랄레논 발생)

  • Jang, Ja Yeong;Kim, Sosoo;Jin, Hyun Suk;Baek, Seul Gi;O, Sujin;Kim, Kyutae;Kim, Jeomsoon;Lee, Theresa
    • Research in Plant Disease
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    • v.24 no.4
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    • pp.308-312
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    • 2018
  • We analyzed rice sample with scab (collected in 2017) for fungal occurrence and found that frequency of Fusarium spp. was the highest among fungal genera. Within Fusarium, Fusarium asiaticum-nivalenol type dominated as 79%. Among mycotoxins, zearalenone was the highest toxin detected (1117 ng/g), followed by deoxynivalenol (163 ng/g), 15-acetyl deoxynivalenol (155 ng/g), nivalenol (110 ng/g). Analysis of mycotoxin levels in scabby and healthy grains (collected in 2015) revealed that both grain samples were contaminated with zearalenone. However, level difference of zearalenone between the samples was over 5 times as the scabby samples were 1400-2370 ng/g, while healthy ones were 240-430 ng/g. This result indicates that scabby grains should be removed when harvest to reduce zearalenone contamination in rice.

The Vascular Plants of Mt. Jigisan in Hongseong-gun, Korea (지기산(홍성군) 일대의 관속식물상)

  • Park, Hwan-Joon;Kim, Jung-Hyun;Kim, Jin-Seok;An, Ji-Hong;Lee, Kyeong-Ui;Hwang, Yo-Seob;Lee, Byoung-Yoon;Nam, Gi-Heum
    • Journal of Environmental Impact Assessment
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    • v.27 no.6
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    • pp.521-547
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    • 2018
  • To provide fundamental information to areas that lack data on the plant resources and the ecosystem, this study conducted a survey on the vascular plants in Mt. Jigisan in Hongseonggun. The vascular plants identified during the six-round field surveys were a total of 443 taxa: 96 families, 286 genera, 391 species, 7 subspecies, 40 varieties, 3 forms, and 2 hybrids. On floristic regional of Korea, Mt. Jigisan is deciduous broad-leaved and conifer-mixed forest which is south province of Korean peninsula. 4 taxa of Korean endemic plants such as Populus ${\times}$ tomentiglandulosa, Forsytsythia koreana, Forsytsythia ovate, and Iris rossii var. latifolia were collected. The vascular plants on the red list according to IUCN evaluation basis were found to be 6 taxa: Vulnerable (VU) species of Forsytsythia ovata, Least Conern (LC) species of Chionanthus retusus, Pseudoraphis ukishiba, and Cymbidium goeringii, and Not Evaluate (NE) species of Panax ginseng, and Fimbristylis verrucifera. The floristic regional indicator plants were found 27 taxa: 2 taxa of grade V, 3 taxa of grade IV, 6 taxa of grade III, 4 taxa of grade II, and 12 taxa of grade I. The specific distribution plants were found 27 taxa: 4 taxa of northern part of plants, 12 taxa of southern part of plants, 10 taxa of isolation distribution of plants, and 1 taxon of recently reported Viola betonicifolia var. albescens. The invasive alien plants were identified as 67 taxa. The naturalized index (NI) was 15.12%, and urbanization index (UI) was 20.9%, respectively.

Vascular Plants Distributed in Ridge of the Northernmost Baekdudaegan Mountains(Hyangrobong~Guryongryeong) (최북단 백두대간(향로봉~구룡령) 능선부에 분포하는 관속식물상)

  • Oh, Hyunkyung;You, Juhan
    • Journal of Environmental Impact Assessment
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    • v.28 no.4
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    • pp.347-372
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    • 2019
  • The purpose of this study is to provide the basic data for management of the ecosystem of Baekdudaegan Mountains by surveying and analysing the vascular plants distributed from Hyangrobong area to Guryongryeong area. The numbers of vascular plants in the whole survey areas were summarized as 633 taxa including 95 families, 321 genera, 549 species, 4 subspecies, 70 varieties, and 10 forms. There were 285 taxa in Hyangrobong area, 256 taxa in Masanbong area, 318 taxa in Mt. Seoraksan area, 281 taxa in Mt. Jeombongsan area, 173 taxa in Bukamryeong and Ohsaekcheon valley area, 197 taxa in Soenadeuri area, 203 taxa in Yeongarigol area, and 168 taxa in Guryongryeong area. The endangered species were 2 taxa including Leontice microrrhyncha and Smilacina bicolor. The rare plants were 39 taxa including Pinus pumila, Halenia corniculata, Juniperus chinensis var. sargentii, and so forth. In IUCN Red List categories, there were 2 taxa of CR, 7 taxa of EN, 10 taxa of VU, 18 taxa of LC, and 2 taxa of DD. The Korean endemic plants were 23 taxa including Salix koriyanagi, Anemone koraiensis, Lespedeza maritima, Cirsium setidens, Saussurea seoulensis, Carex okamotoi, and so forth. The plants adaptable to climate change were 48 taxa including 17 taxa of endemic plants, 1 taxon of southern plant, and 30 taxa of northern plants. The naturalized plants were 32 taxa including Fallopia dumetorum, Cerastium glomeratum, Papaverrhoeas, Bidensfrondosa, Lolium perenne, and so forth. The invasive alien plants were 3 taxa including Rumex acetocella, Ambrosia artemisiifolia, and Ambrosia trifida.

Modulation of Intestinal Microbiota by Supplementation of Fermented Kimchi in Rats (발효 김치가 흰쥐의 장내 미생물 형성에 미치는 영향)

  • An, Su Jin;Kim, Jae Young;Kim, In Sung;Adhikari, Bishnu;Yu, Da Yoon;Kim, Jeong A;Kwon, Young Min;Lee, Sang Suk;Choi, In Soon;Cho, Kwang Keun
    • Journal of Life Science
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    • v.29 no.9
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    • pp.986-995
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    • 2019
  • Intestinal microbiota play a key role in maintaining the host's health, and variety and richness of this microbiota is directly influenced and modulated by the host's diet. Kimchi is a fermented food rich in dietary fibers and lactic acid bacteria (LAB). To investigate the effect of fermented kimchi on the host's response and the composition of intestinal microbiota, 45 male Sprague-Dawley rats six weeks old were divided into three experimental groups that received either a basal diet (CON) or a basal diet supplemented with fermented kimchi (FK) or chitosan-added fermented kimchi (CFK) for four weeks. Body weights and feed intakes were measured weekly, and the intestinal contents were collected aseptically and were used for 16S rRNA gene profiling via pyrosequencing. As compared to the control, FK and CFK groups showed less body weight gain, feed efficiency, and blood triglyceride concentration. The diversity of intestinal microbiota was increased in both FK and CFK as compared to the control. At the phylum level, obesity-associated Firmicutes decreased, while leanness-associated Bacteroidetes increased. At the genus-level, the genera that consist of LAB, leanness-associated bacteria, and butyric acid-producing bacteria increased in FK and CFK as compared to the control. The overall results suggest that the consumption of fermented kimchi can reduce obesity and promote the host's health through mechanisms involving the modulation of intestinal microbiota.

Density and Distribution of the Mosquito Population Inhabiting Jeju Region, 2018 (제주지역에 서식하는 모기 개체군 밀도와 분포, 2018)

  • Seo, Min Young;Chung, Kyoung A
    • Korean Journal of Clinical Laboratory Science
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    • v.51 no.3
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    • pp.336-343
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    • 2019
  • In order to investigate the density of seasonal incidence of mosquitoes, a vector of infectious diseases in Jeju region, this study collected mosquitoes using a Black light trap (BL) and Biogents' Sentinel 2 Mosquito Trap (BG), dividing the region into cattle sheds, habitats for migratory birds, and the downtown area, twice a month for 9 months from March through November 2018. Then, this study conducted identification and classification and checked for the presence of Flavivirus using reverse transcription polymerase chain reaction (RT-PCR). As for the mosquito population, 1,847 mosquitoes (six genera, 12 species) were collected. The places where most mosquitoes were collected were copses near craft workshops in habitats for migratory birds and Jungang-dong in the Seogwipo downtown area. For the population, Culex pipiens pallens was the dominant species (76.9%), followed by Aedes albopictus (8.9%). Most of the population was collected in June, followed by August and October. This study conducted a RT-PCR test with 1,847 collected mosquitoes, which were divided into 50 pools if they had Flavivirus. All turned out to be negative. However, the results of the investigation show the presence of Culex tritaineniorhychus, Aedes albopictus, and Anopheles sinensis and can be used as a basis for the comprehensive prevention management of mosquitoes.

Recent advances on next-generation probiotics linked to the gut microbiome (장내 마이크로바이옴과 차세대 프로바이오틱스 연구 현황)

  • Choi, Hak-Jong
    • Food Science and Industry
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    • v.52 no.3
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    • pp.261-271
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    • 2019
  • Gut microbiome have recently provided evidence that the gut microbiota are capable of greatly influencing all aspects of physiology and immunology. Although a number of recent studies have shown that probiotics can modulate gut microbiota structure, the mechanism underlying this effect remains to be elucidated. In a disease state, the relative abundances of beneficial gut bacteria are generally reduced, which is restored by constant probiotic supplementation. Oral administration of probiotics improved the disease state by (1) inducing differentiation and function of regulatory T cells, (2) reducing inflammatory response, (3) modulating the gut environment, and (4) increasing the proportions of short-chain fatty acid- or beneficial metabolite-producing gut microbiota including the genera Bifidobacterium, Faecalibacterium, Akkermansia, etc. In this review, current knowledge on how probiotics can influence host's health by altering gut microbiota structure and on how probiotics and beneficial gut bacteria can be applied as next-generation probiotics will be discussed.

Floristic study of Jeonggwangsan Mt. (Yongin, Korea) (정광산(용인시)의 관속식물상)

  • JEON, Ji-Hyeon;CHO, Myong-Suk;GIL, Hee-Young;KIM, Seon-Hee;JUNES, Petra;SEO, Hee-Seung;KIM, Seung-Chul
    • Korean Journal of Plant Taxonomy
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    • v.49 no.1
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    • pp.70-89
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    • 2019
  • In this study, we investigated the flora of Jeonggwangsan Mt. (Yongin, Korea) from May of 2015 to September of 2016. Through 14 independent field investigations, we identified a total of 367 taxa of vascular plants, including 89 families, 234 genera, 323 species, six subspecies, 34 varieties, and four formae. Eight taxa were endemic to Korea, while 27 were naturalized in Korea. Nineteen taxa were the floristic regional indicator plants in Korea: four in Rank VI, four in Rank III, two in Rank II, and nine in Rank I. A total of 59 taxa were designated as controlled Korean biological resources, requiring special permission for export to outside countries. Two taxa were listed on the Red List of Korea, including one Near Threatened (NT) taxon (Hololeion maximowiczii Kitam.) and one Least Concern (LC) taxon (Iris minutoaurea Makino).

The Effects of Self-Esteem, Ego Resilience, Impulsivity and Stress on Smartphone Addiction among College Students (대학생의 자아 존중감, 자아탄력성, 충동성, 스트레스가 스마트폰 중독에 미치는 영향)

  • Kim, Jong-Im
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.20 no.7
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    • pp.192-202
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    • 2019
  • This study was a descriptive study to investigate the factors influencing on self-esteem, ego resilience, impulsivity and life stress on smartphone addiction among college students. The subjects include college students in some areas. Data was collected in November and December, 2018. Total 231 college students were analyzed in the study. Collected data was analyzed in frequency, percentage, and t-test to examine differences in smartphone addiction according to general characteristics. Pearson's correlation was performed to examine correlations between smartphone addiction and main variables, and the factors influencing smartphone addiction were analyzed in multiple regression. The findings show that genera, satisfaction with college life, hours of using a smartphone, hours of using SNS, ego resilience, impulsivity, and life stress had significant effects on smartphone addiction. The study examined relations between general characters and smartphone addiction and found that the level of smartphone addiction was high in female college students, those who were not satisfied with college life, and used a smartphone and SNS for many hours. Ego resilience had negative correlations with smartphone addiction, and impulsivity and life stress had positive correlations with it. Multi-variate regression analysis results show that gender, ego resilience, and life stress were factors that had important influences on the smartphone addiction of college students, having explanatory power of 35.6%. In short, colleges and universities need to develop a school-level program to improve the college life of students and reduce their stress to the minimum and create an environment of encouraging them to grow power of controlling their lives so that they can prevent smartphone addiction.

The Characteristics of Vascular Plants Distributed in Royal Tomb of the Joseon Dynasty - Focused on Gwangneung - (조선왕릉에 분포하는 관속식물상의 특성 - 광릉을 중심으로 -)

  • Oh, Hyun-Kyung;Kim, Eun-Ok;You, Ju-Han
    • Journal of the Korean Institute of Traditional Landscape Architecture
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    • v.37 no.1
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    • pp.41-55
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    • 2019
  • The purpose of this study is to present the basic data for conservation and management of traditional landscape and ecological environment by surveying and analysing the vascular plants distributed in Gwnagneung, a royal tomb of the Joseon Dynasty. The results are as follows. The numbers of vascular plants were summarized as 446 taxa including 97 families, 274 genera, 384 species, 4 subspecies, 49 varieties and 9 forms. The planting species were 34 taxa including Ginkgo biloba L., Pinus koraiensis Siebold & Zucc., Taxus cuspidata Siebold & Zucc. and so forth. Exotic species such as Pterocarya stenoptera DC. and Magnolia obovata Thunb. need to be remove for traditional landscape management. The rare plants were 7 taxa including 2 taxa of CR(critically endangered), 2 taxa of VU(vulnerable) and 3 taxa of LC(least concerned). The Korean endemic plants were 8 taxa including Pseudostellaria coreana (Nakai) Ohwi, Chrysosplenium pilosum var. fulvum (N.Terracc.) Hara, Philadelphus schrenkii Rupr., Indigofera koreana Ohwi, Paulownia coreana Uyeki(planting), Weigela subsessilis (Nakai) L.H.Bailey, Aster koraiensis Nakai(planting) and Hosta minor (Baker) Nakai(planting). The specific plants by floristic region were 45 taxa including 2 taxa of grade V, 12 taxa of grade III, 9 taxa of grade II and 22 taxa of grade I. The naturalized plants were 25 taxa including Fallopia dumetorum (L.) Holub, Chenopodium ficifolium Smith, Amorpha fruticosa L., Galinsoga ciliata (Raf.) S.F.Blake and so forth. The target plants adaptable to climate change were 9 taxa including 1 taxon of endemic plant and 8 taxa of northern plants.