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The Number of Nucleotide Substitutions per Sites of Mitochondrial DNA in the Four Pleuronectid Species (미토콘드리아 DNA에 의한 붕넙치과 어류 4종간의 염기치환수)

  • PARK Jung-Youn;KIM Yoon
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.28 no.5
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    • pp.649-658
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    • 1995
  • In order to estimate the level of genetic differences among the pleuronectid species, mitochondrial DNAs were isolated from four species: brown sole, Limanda herensteini; marbled sole, Limanda Yokohamae; stone flounder, Kareius bicoloratus; starry flounder, Platichthys stellatus, and the number of nucleotide substitutions was calculated by the restriction fragment length polymorphisms (RFIPs) generated by f4 sin base recognition restriction endonucleases. Total lengths of the mitochondrial DNA were measured as about 17.6 kbp in all species. Ten different composite genotypes were observed in brown sole, four different genotypes in marbled sole, and two different genotypes in starry flounder. However, only one genotype was observed in stone flounder. The calculated haplotypic diversity value of brown sole was higher than that of marbled sole. The average number of nucleotide substitutions per sites in four species was estimated to be 0.0045 in the intraspecies, 0.0344 in the interspecies, and 0.0457 in the genera, respectively. From these results, we could estimate that the genetic differences among interspecies were not influenced by nucleotide substitutions but genetical discontinuous.

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Diversity and physiological properties of soil actinobacteria in Ulleung Island (울릉도 유래 토양 방선균의 다양성과 생리활성)

  • Yun, Bo-Ram;Roh, Su Gwon;Kim, Seung Bum
    • Korean Journal of Microbiology
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    • v.53 no.4
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    • pp.242-250
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    • 2017
  • Actinobacteria tolerating extreme conditions can be a rich source of bioactive compounds and enzymes. In this study filamentous actinobacteria were isolated from soils of Ulleung Island, and their physiological properties were examined. Soil samples were collected, serially diluted and spread on various agar media. The average viable counts of total bacteria were $1.28{\times}10^7CFU/g$ for soil sample 1 (ULS1) and $2.05{\times}10^7CFU/g$ for soil sample 2 (ULS2). As a result, 34 strains of actinobacteria were isolated and assigned to the genera Streptomyces (16 strains), Isoptericola (5 strains), Rhodococcus (4 strains), Agromyces (3 strains), Micrococcus (2 strains), Arthrobacter (1 strain), Williamsia (1 strain), Microbacterium (1 strain), and Oerskovia (1 strain) based on 16S rRNA gene sequence analysis. Enzyme activity and plant growth promoting potential were tested for representative isolates. Multiple strains of Streptomyces degraded starch, casein and Tween 80. As for plant growth promoting potential, strains of Oerskovia, Williamsia, Isoptericola, and Streptomyces solubilized phosphate, and those of Agromyces, Oerskovia, Micrococcus, Rhodococcus, Streptomyces, and Isoptericola produced 3-indole-acetic acid (IAA), respectively. Selected strains of Streptomyces exhibited strong antagonistic activity against Staphylococcus aureus and Bacillus subtilis as well as Candida albicans. This study confirms that actinobacteria from Ulleung Island can be a good source of novel bioactive compounds.

Effect of Species and Volume Rate of Potted Orchid Plants Put in Closed Chambers on Emission of Air Anions (난의 종류 및 공간대비 투입 비율에 따른 음이온 발생량 구명)

  • Kim, Kwang Jin;Lee, Jong Suk
    • FLOWER RESEARCH JOURNAL
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    • v.16 no.1
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    • pp.85-92
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    • 2008
  • The air anions generated by potted Cymbidium spp., Cymbidium Meglee 'Ms Taipei' Dendrobium phalaenopsis, Oncidium spp., Phalaenopsis spp., and Sedirea japonicum were investigated, and changes of air anion emission were measured as affected by volume rates of potted orchids put in chambers. The volume rates of 20, 40, 60, and 80% for potted Cymbidium spp. and 25, 50, 75, and 100% for potted Cymbidium Meglee 's Taipei'were used in closed chambers. Air anion emission by Cymbidium spp. ($332/cm^3$) and Cymbidium 's Taipei'($323/cm^3$) was the greatest among the tested six orchid species, followed by Dendrobium phalaenopsis ($250/cm^3$), Oncidium spp. ($203/cm^3$), Sedirea japonicum ($119/cm^3$), and Phalaenopsis spp. ($77/cm^3$). Air anion emission by Cymbidium spp. and Cymbidium Meglee 'Ms Taipei'g increased by 40% and 75%, respectively, and thereafter slightly decrease. Therefore, the volume rates of potted plants put in closed chambers should be less than 40% to compare with species and 30% seems most pertinent considering increasing relative humidity in closed chambers. Air anions were generated the greatest amount by potted Cymbidium genera among the orchids tested.

The Flora in Tributary Region of Middle Stream of the Nakdong River (Young-gang, Naeseong-cheon, Wi-cheon, Gam-cheon and Byeongseong-cheon) (낙동강 중류 지천의 식물상(영강, 내성천, 위천, 감천, 병성천))

  • Jeong, Ji-Hyeon;Hong, Sun-Cheon;Park, Hee-Jun;Paik, Weon-Ki
    • Korean Journal of Plant Resources
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    • v.32 no.5
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    • pp.615-632
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    • 2019
  • Field surveys were conducted from May 2016 to October and from April to October 2017 in Tributary Region of Middle Stream of the Nakdong River. The survey was carried out at 66 sites including Young-gang 11, Naeseong-cheon 21, Wi-cheon 16, and Gam-cheon 9, Byeongseong-cheon 9. As a result of investigation, 474 taxa in total ; 99 familly, 288 genera, 421 species, 4 subspecies, 39 varieties and 10 forma. Among them, 5 Korean endemic plants, 7 rare plants, 31 taxa were listed as Floristic Target Species in Korea. Naturalized plants was confirmed 73 taxa, their Urbanization Ratio 22.7%, and naturalization ratio 15.4% in the investigated area. The Life form(Dormancy form) review results as follows ; Therophytes 159 taxa, Hemicryptophytes 91 taxa, Hydrophanerophytes 68 taxa, Geophyte 52 taxa, Nanophanerophytes 35 taxa, Megaphanerophytes 32 taxa, Microphanerophytes 25 taxa, Chamaephytes 12 taxa, and Epiphyten 1 taxa.

Phylogeny of the family Ophioglossaceae with special emphasis on genus Mankyua (제주고사리삼을 중심으로한 고사리삼과 식물의 계통)

  • Sun, Byung-Yun;Baek, Tae Gyu;Kim, Young-Dong;Kim, Chan Soo
    • Korean Journal of Plant Taxonomy
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    • v.39 no.3
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    • pp.135-142
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    • 2009
  • Phylogeny of the family Ophioglossaceae and a phylogenetic position of Mankyua were estimated through analyses of chloroplast rbcL gene sequences and spore morphology. Sequence analysis of the rbcL gene clearly indicated that there are two major lineages in the family Ophioglossaceae: Botrychioid lineage and Ophioglossoid lineage. The Botrichioid lineage is composed of three distinct clades: Botrychium, Helminthostachys and Mankyua, where Helminthostachys and Mankyua were placed as sister groups to the Botrychium. Within the genus Botrychium, subgenera Septridium and Botrychium were monophyletic, while taxa of subgen. Botrypus branched as sister of the two, successively, thus making a non-monophyletic group. Ophioglossum formed the Ophioglossoied lineage, where the subgen. Ophioglossum is monophyletic, while subgen. Cheiroglossa and Ophoderma formed a sister relationship with subgen. Ophioglossum. In terms of external morphology and spores, Mankyua is most similar to Helminthostachys, however, patristic distance in the cladogram and trophophore characteristics of the two genera are distinct. Therefore, Mankyua is a well defined genus within the family in terms of morphology as well as molecular phylogeny which places it in basal position of the Botrychioid lineage on the gene tree.

Floristic study of Mt. Baekasan (백아산의 식물상)

  • Sun, Eun-Mi;Kim, Byeol-Ah;Son, Hyun-Deok;Jeong, Jeong-Chae;Im, Hyoung-Tak
    • Korean Journal of Plant Taxonomy
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    • v.47 no.3
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    • pp.236-263
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    • 2017
  • This study presents a discussion of vascular plants found on Mt. Baekasan, Hwasun-gun, Jeollanam-do, based on specimens collected during 32 separate field trips amounting to a total of 36 days conducted between May of 2011 and October of 2016. There are 651 taxa on Mt. Baekasan, consisting of 109 families, 366 genera, 576 species, 5 subspecies, and 70 varieties. Twelve plants endemic to Korea were found, including Cirsium setidens (Dunn) Nakai and Scutellaria insignis Nakai. There are a total of 61 taxa of floristic regional indicator plants specially designated by the Ministry of the Environment. Rare plants such as Jeffersonia dubia (Maxim.) Benth. & Hook. ex Baker & S. Moore in Mt. Baekasan are 17 taxa in total, and red list plants number 8 taxa, including Cremastra variabilis (Blume) Nakai ex Shibata. Nine calciferous plants were found, including Asplenium ruprechtii Sa. Kurata, Ulmus davidiana var. japonica (Rehder) Nakai and Morus cathayana Hemsl. Thirty-three naturalized plants were also found, including Ambrosia artemisiifolia and Rumex acetosella.

The Vascular Plants in Mt. Geotdae, Cheongju-si, Chungcheongbuk-do (충청북도 청주시 것대산 일대에 분포하는 관속식물상)

  • Jeoung, Tae-Young;Park, Cheol-Ha;Yun, Hee-Bin;Lee, Gwi-Yong;Ahn, Chan-Ki;Lee, Kyung-Soo;Kim, Kyung-Tae;Lee, Woo-Sung;You, Ju-Han
    • Korean Journal of Plant Resources
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    • v.20 no.5
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    • pp.451-460
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    • 2007
  • This study was carried out to construct the data base for biodiversity conservation, and offer the raw data for obtaining the genetic resources. The vascular plants were summarized as 306 taxa; 79 families, 199 genera, 266 species, 35 varieties and 5 forma. The Korean endemic plants were 7 taxa; Salix caprea, S. purpurea var. japonica, Deuitzia coreana, Akebia quinata var. plyphylla, Spiraea prunifolia for. simpiciflora, Lonicera subsessilis and Weigela subsessilis. The naturalized plants were 16 taxa; Dactylis glomerata, Rumex acetocella, R. crispus, Phytolacca esculenta, Tlaspi arvense, Robinia pseudo-acacia, Trifolium repens, Ailanthus altissima, Oenothera lamarckiana, Veronica persica, Ambrosia artemisiifolia var. elatior, Erigeron annuus, Erigeron canadensis, Bidens frondosa, Taraxacum officinale and Sonchus asper. The specific plants by floristic region were 26 taxa; Chloranthus japonica, Populus davidiana, and so forth.

Floristic Study of Neunggyeong-bong (Pyeongchang-gun, Gangneung-si) in Korea (능경봉(평창군, 강릉시)의 관속식물상)

  • Kim, Hyun-Jun;Joo, Minjung;Ji, Seong-Jin;So, Soonku;Jung, Su Young;Chang, Kae Sun;Choi, Kyung;Yang, Jong Cheol
    • Korean Journal of Plant Resources
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    • v.28 no.2
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    • pp.178-192
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    • 2015
  • This study was carried out to investigate the vascular plants of Neunggyeong-bong, Gangwond-do, South Korea. The vascular plants that were collected 7 times from 2008 and 2013 consisted a total of 440 taxa; 75 families, 261 genera, 383 species, 4 subspecies, 48 varieties and 5 forms. Among them, 15 taxa of Korean endemic plants and 11 taxa of rare and endangered plants were included. The floristic regional indicator plants found in this area were 11 taxa of grade IV, 22 taxa of grade III. Naturalized plants consisted of 29 taxa that made up 6.6% of the total vascular plants in this area. Medicinal plants of the Korean Pharmacopoeia and Korean Herbal Pharmacopoeia distributed in this area consisted of 60 taxa.

The Vascular Plants in Mt. Bohyeon, Gyeongbuk, Korea (보현산(경북)의 관속식물상 연구)

  • Park, Kyu Tae;Choi, Kyoung Su;Son, Ogyeong;Lee, Eun Mi;Kim, Hae Sik;Boo, Da Un;Park, SeonJoo
    • Korean Journal of Plant Resources
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    • v.28 no.2
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    • pp.193-216
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    • 2015
  • This study was carried out to investigate the vascular plants of Mt. Bohyeon (Gyeongbuk). The vascular plants were collected 22 times and summarized as 589 taxa, including 105 families, 327 genera, 522 species, 11 subspecies, 48 varieties and 8 forms respectively. Among them, 13 Korean endemics, 10 red list of vascular plants, 2 endangered plants and 83 specially designated plants by the Ministry of Environment were included respectively. The naturalized plants were 37 taxa and the naturalized ratio was 6.41 of 589 taxa. Usage of 589 taxa were consists of 240 taxa (40.1%) of edible plants, 205 taxa (34.2%) of medicinal plants, 62 taxa (10.3%) of ornamental plants, 213 taxa (35.6%) of pasture plants, 15 taxa (2.5%) of timber plants, 8 taxa (1.3%) of fiberplants, and 3 taxa (0.5%) of industrial plants.

Vegetation of wetland in Mueuido(Incheon-city) (무의도(인천시)의 습지 식생)

  • Paik, Weon-Ki
    • Korean Journal of Plant Resources
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    • v.23 no.2
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    • pp.197-205
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    • 2010
  • This study was carried out to investigate the flora and vegetation in wetland of Mueuido. The forest vegetation was classified into 2 communities group (Utricularia bifida-Eriocaulon sikokianum and Alnus japonica) and 6 communities (Rhynchospora chinensis, Phragmites communis, Molinia japonica, Ischaemum crassipes, Quercus serrata and Pinus rigida-Miscanthus sinesis). Resources vascular plants were composed of 30 families, 53 genera, 50 species and 8 varieties, totaling 58 taxa. Among the investigated resources plants, Utricularia bifida and Utricularia racemosa were rare and endangered species. The special plants based on floral region by Ministry of Environment were 5 taxa; V rank species 2 taxa, II rank species 1 taxa and I rank species 2 taxa. A naturalized plants was 1 species of totaling 290 species appeared in South Korea. The Life-form were Hydatophytes (20.7%), Geophyte (19%), Microphanerophytes (19%), Hemicryptophytes (17.2%), Nanophanerophytes (17.2%), Chamaephytes (5.2%), Therophytes (1.7%). In marsh-type wetland of Mueuido, we predict that bog-type swamp, where various type of swamp plants are distributed, is starting to be formed based on the fact that Utricularia bifida and Utricularia racemosa, insectivorous plants, are dominant and sphagnum also is found. Along with the notion, there are reportedly found serious damages around wetland by human being and we have to prepare protection plans against it.