Browse > Article
http://dx.doi.org/10.7845/kjm.2017.7057

Diversity and physiological properties of soil actinobacteria in Ulleung Island  

Yun, Bo-Ram (Department of Microbiology and Molecular Biology, College of Bioscience and Biotechnology, Chungnam National University)
Roh, Su Gwon (Department of Microbiology and Molecular Biology, College of Bioscience and Biotechnology, Chungnam National University)
Kim, Seung Bum (Department of Microbiology and Molecular Biology, College of Bioscience and Biotechnology, Chungnam National University)
Publication Information
Korean Journal of Microbiology / v.53, no.4, 2017 , pp. 242-250 More about this Journal
Abstract
Actinobacteria tolerating extreme conditions can be a rich source of bioactive compounds and enzymes. In this study filamentous actinobacteria were isolated from soils of Ulleung Island, and their physiological properties were examined. Soil samples were collected, serially diluted and spread on various agar media. The average viable counts of total bacteria were $1.28{\times}10^7CFU/g$ for soil sample 1 (ULS1) and $2.05{\times}10^7CFU/g$ for soil sample 2 (ULS2). As a result, 34 strains of actinobacteria were isolated and assigned to the genera Streptomyces (16 strains), Isoptericola (5 strains), Rhodococcus (4 strains), Agromyces (3 strains), Micrococcus (2 strains), Arthrobacter (1 strain), Williamsia (1 strain), Microbacterium (1 strain), and Oerskovia (1 strain) based on 16S rRNA gene sequence analysis. Enzyme activity and plant growth promoting potential were tested for representative isolates. Multiple strains of Streptomyces degraded starch, casein and Tween 80. As for plant growth promoting potential, strains of Oerskovia, Williamsia, Isoptericola, and Streptomyces solubilized phosphate, and those of Agromyces, Oerskovia, Micrococcus, Rhodococcus, Streptomyces, and Isoptericola produced 3-indole-acetic acid (IAA), respectively. Selected strains of Streptomyces exhibited strong antagonistic activity against Staphylococcus aureus and Bacillus subtilis as well as Candida albicans. This study confirms that actinobacteria from Ulleung Island can be a good source of novel bioactive compounds.
Keywords
Streptomyces; actinobacteria; antimicrobial activity; plant growth promoting potential; Ulleung Island;
Citations & Related Records
Times Cited By KSCI : 2  (Citation Analysis)
연도 인용수 순위
1 Atalan, E., Manifio, G.P., Ward, A.C., Kroppenstedt, R.M., and Goodfellow, M. 2000. Biosystematic studies on novel streptomycetes from soil. Antonie van Leeuwenhoek 77, 337-353.   DOI
2 Balachandran, C., Duraipandiyan, V., and Ignacimuthu, S. 2012. Purification and characterization of protease enzyme from actinomycetes and its cytotoxic effect on cancer cell line (A549). Asian Pac. J. Trop. Biomed. 2, 392-400.   DOI
3 Busarakam, K., Girard, G., and Labeda, D. 2014. Streptomyces leeuwenhoekii sp. nov., the producer of chaxalactins and chaxamycins, forms a distinct branch in Streptomyces gene trees. Antonie van Leeuwenhoek 105, 849-861.   DOI
4 Criquet, S. 2002. Measurement and characterization of cellulase activity in sclerophyllous forest litter. J. Microbiol. Method 50, 165-173.   DOI
5 Dasari, V., Muthyala, K., Nikku, M., and Donthireddy, S. 2012. Novel pyridinium compound from marine actinomycete, Amycolatopsis alba var. nov. DVR D4 showing antimicrobial and cytotoxic activities in vitro. Microbiol. Res. 167, 346-351.   DOI
6 Demain, A. and Sanchez, S. 2009. Microbial drug discovery: 80 years of progress. J. Antibiot. 62, 5-16.   DOI
7 Gesheva, V. and Gesheva, R. 2000. Physiological and antagonistic potential of actinomycetes from loquat rhizosphere. Microbiol. Res. 155, 133-135.   DOI
8 Han, S.I. 2015. Phylogenetic characterization of bacterial populations in different layers of oak forest soil. Korean J. Microbiol. 51, 133-140.   DOI
9 Glickmann, E. and Dessaux, Y. 1995. A critical examination of the specificity of the Salkowski reagent for indolic compounds produced by phytopathogenic bacteria. Appl. Environ. Microbiol. 61, 793-796.
10 Goodfellow, M. 2012. Class I. Actinobacteria stackebrandt, rainey and ward-rainey 1997, 483. pp. 34-1967. In Goodfellow, M., Kampfer, R., Busse, H.J., Trujillo, M.E., Suzuki, K.I., Ludwig, W., and Whitman, W.B. (eds.) Bergey's Manual of Systematic Bacteriology, 2nd ed. Springer, New York, USA.
11 Han, S.I., Cho, M.H., and Whang, K.S. 2008. Comparison of phylogenetic characteristics of bacterial populations in a Quercus and pine humus forest soil. Korean J. Microbiol. 44, 237-243.
12 Jeon, Y.S., Lee, K., Park, S.C., Kim, B.S., Cho, Y.J., Ha, S.M., and Chun, J. 2014. EzEditor: a versatile sequence alignment editor for both rRNA-and protein-coding genes. Int. J. Syst. Evol. Microbiol. 64, 689-691.   DOI
13 Jukes, T.H. and Cantor, C.R. 1969. Evolution of protein molecules, pp. 21-132. In Munro, H.N. (ed.), Mammalian Protein Metabolism. Academic Press, New York, USA.
14 Khamma, S., Yokota, A., and Lumyong, S. 2008. Actinomycetes isolated from medicinal plant rhizosphere soils: diversity and screening of antifungal compounds, indole-3-acetic acid and siderophore production. Microbiol. Res. 25, 649-655.
15 Kim, O.S., Cho, Y.J., Lee, K.H., Yoon, S.H., Kim, M.C., Na, H.S., Park, S.C., Jeon, Y.S., Lee, J.H., Yi, H.N., et al. 2012. Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int. J. Syst. Evol. Microbiol. 62, 716-721.   DOI
16 Ouhdouch, Y. and Barakate, M. 2001. Actinomycetes of moroccan habitats: isolation and screening for antifungal activities. Eur. J. Soil Biol. 37, 69-74.   DOI
17 Kim, T.S., Han, J.H., Joung, Y.C., and Kim, S.B. 2014. Conyzicola lurida gen. nov., sp. nov., isolated from the root of Conyza canadensis. Int. J. Syst. Evol. Microbiol. 64, 2753-2757.   DOI
18 Kumar, S.M., Dhahagani, K., Chakkaravarthi, G., Anitha, K., Rajesh, J., Ramu, A., and Rajagopal, G. 2014. Synthesis and spectral characterization of Schiff base complexes of Cu(II), Co(II), Zn(II) and VO(IV) containing 4-(4-aminophenyl)morpholine derivatives: antimicrobial evaluation and anticancer studies. Spectrochim. Acta Part A Mol. Biomol. Spetrosc. 117, 87-94.   DOI
19 Leong, J. 1996. Siderophores: their biochemistry and possible role in the biocontrol of plant pathogens. Annu. Rev. Phytopathol. 24, 187-209.
20 Pandey, A. and Palni, L.M.S. 2007. The rhizosphere effect in trees of the Indian Central Himalaya with special reference to altitude. Appl. Ecol. Environ. Res. 5, 93-102.
21 Park, D.J., Lee, S.H., and Kim, C.J. 1998. Seasonal change of microbial population in the field soil. Korean J. Microbiol. 34, 144-148.
22 Saitou, N. and Nei, M. 1987. The neighbor-joining method: A new method for reconstructing phylogenetic trees. Mol. Biol. Evol. 4, 406-425.
23 Schwyn, B. and Neilands, J.B. 1997. Universal chemical assay for the detection and determination of siderophores. Anal. Biochem. 160, 46-56.
24 Thangapandian, V., Ponmuragan, P., and Ponmuragan, K. 2007. Actinomycetes diversity in the rhizosphere soil of different medicinal plants in Kolly Hills Termilnadu, India, for secondary metabolite production. Asian J. Plant Sci. 6, 66-70.   DOI
25 Shirling, E.B. and Gottlieb, D. 1966. Methods for characterization of Streptomyces species 1. Int. J. Syst. Evol. Microbiol. 16, 313-340.
26 Sulbaran, M., Pérez, E., Ball, M., and Bahsas, A. 2009. Characterization of the mineral phosphate-solubilizing activity of Pantoea aglomerans MMB051 isolated from an iron-rich soil in southeastern Venezuela (Bolívar State). Mol. Biol. Evol. 58, 378-383.
27 Tamura, K., Stecher, G., Peterson, D., Filipski, A., and Kumar, S. 2013. MEGA6: molecular evolutionary genetics analysis version 6.0. Mol. Biol. Evol. 30, 2725-2729.   DOI
28 Teachowisan, T., Peberdy, J.F., and Lumyong, S. 2003. Isolation of endophytic actinomycetes from selected plants and their antifungal activity. World J. Microbiol. Biotechnol. 19, 381-385.   DOI
29 Tewtrakul, S. and Subhadhirasakul, S. 2007. Anti-allergic activity of some selected plants in the Zingiberaceae family. J. Ethnopharmacol. 109, 535-538.   DOI