• Title/Summary/Keyword: gamma-irradiated

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Studies on the Manufacturing of Sujeonggwa (Korean Traditional Cinnamon Flavored Persimmon Punch) Edible in Severe Environment by Gamma Irradiation (감마선 조사기술 이용 극한환경에서도 취식 가능한 수정과 제조에 관한 연구)

  • Park, Jae-Nam;Lee, Ju-Woon;Kim, Jae-Hun;Kim, Kwan-Soo;Han, Kyu-Jai;Sul, Min-Sook;Lee, Hyun-Ja;Byun, Myung-Woo
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.36 no.5
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    • pp.609-615
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    • 2007
  • This study was conducted to develop the method for the safe supply of Sujeonggwa (cinnamon flavored persimmon punch) in severe environments such as space, desert or deep sea, by the combined treatment of gamma irradiation with other food technologies. Commercially prepared Sujeonggwa powder could be sterilized at 4.5 kGy or above doses. However, sensory characteristics of gamma-irradiated Sujeonggwa decreased depending upon the dose. The combined treatment of vacuum packaging with the addition of vitamin C and cinnamic aldehyde in Sujeonggwa powder could minimize the change of sensory qualities induced by ionizing irradiation.

ESR Study of Paramagnetic Defects of the ${\gamma}$-irradiated Potassium Sulfate Single Crystal (${\gamma}$-선에 조사된 황산칼륨 단결정의 상자성 결함에 관한 전자스핀공명 연구)

  • Yo Chul Hyun;Chung Won Yang;Jong In Hong;Eun Ok Kim;Jung Sung Yang
    • Journal of the Korean Chemical Society
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    • v.25 no.6
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    • pp.367-375
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    • 1981
  • Single crystals of Potassium Sulfate ($K_2SO_4$) have been grown from the saturated solution by the evaporation method at the optimum conditions. Radiation damages in the crystal by ${\gamma}$-irradiation of about $12{\times}10^6$ Roentgen have given rise to paramagnetic centers or paramagnetic defects. Electron spin resonance (ESR) spectra of the centers are obtained with the X-band EPR spectrometer at room temperature. The ESR peaks of the paramagnetic species are found to be anisotropic but the peak of $SO_3-$ radical is an isotropic of Gaussian shape at g = 2.0036. A number of ESR spectra of the crystal for angular variation of the anisotropic peaks are recorded at various orientations of rotation about a, b and c crystallographic axes respectively. The g-values are calculated from the line position between anisotropic peaks and the isotropic one and then principal g-values and its direction cosines of the species are obtained by diagonalization of 9 matrix elements of the corresponding g-values. All the paramagnetic defects are identified by the characteristic principal g-values and its direction cosines.

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Effect of Gamma Irradiation on the Sterilization of Red Pepper Powder (고추가루의 살균(殺菌)을 위한 ${\gamma}$-선(線) 조사효과)

  • Kwon, Joong-Ho;Byun, Myung-Woo;Cho, Han-Ok
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.13 no.2
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    • pp.188-192
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    • 1984
  • In order to investigate the hygienic status of commercial red pepper powder, one sample was directly prepared from raw red pepper which was purchased at market and the other one was a commercial red pepper powder. They were used for the investigation upon the status of microbial contamination, effect of irradiation for sterilization and physicochemical changes during 3 months of storage. Total bacterial counts of commercial red pepper powders were 3.83 to $6.68{\times}10^6$ per gram and coliform group was shown to be positive in some products. Total bacteria and coliform group were sterilized by the irradiation of 9 kGy and 3 kGy, respectively and no microorganisms were grown up until 3 months of storage at room temperature. The $D_{10}$ values of total viable bacteria in red pepper powders were 1.52 to 1.58 kGy. Chemical components such as moisture, total and reducing sugars were slightly decreased during the storage period. The contents of capsanthin and capsaicin were partly affected by the high dose irradiation but the difference between unirradiated and irradiated groups was diminished with the elapse of storage period.

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Gamma Radiation Sensitivity and Quantitative Characters in M1 Generation of Heteropappus hispidus (Thunb.) (갯쑥부쟁이(Heteropappus hispidus (Thunb.))의 감마방사선 감수성과 M1 세대 형질변동 특징)

  • Oh, Byung-Kwon;Hong, Kyung-Ae;Song, Sung-Jun;Lee, Sun-Joo;Lee, Young-Il;U., Zang-Kual
    • Korean Journal of Environmental Agriculture
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    • v.22 no.4
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    • pp.294-299
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    • 2003
  • The seeds of naturally growing Heteropappus hispidus (Thunb.) were treated by nine different doses (0, 10, 20, 40, 80, 120, 160, 200, 300, 400 Gy) of gamma rays to investigate their germination rate and to quantity the characteristics of their germinated plants as like as leaf appearance and length, the formation rate of anthocyanin color in stem 30 days after germination, the formation rate of rosette leaf and multi-shoot, the flowering and seed-bearing, and shoot length. The germination rate at least up to 120Gy was not greatly affected but was rapidly decreased at over 160Gy. It seemed that lethal dose ($LD_{50}$) of germination was 160Gy. The leaf appearance and growth was also inhibited, but the formation rate of anthocyanin color in the flower stem was enhanced up to 30% with dose. The rosette plants were observed in plants irradiated with higher than 40Gy. Multi-shoots were developed over 80Gy. For a short shoot length and bundle of thin stem, it was considered that they can be selected as the potential pot flower plants, through genetic fixation. In particular, it was suggested that the formation of anthocyanin color in flower stem, rosette and multi-shoot plants induced by the high dose of gamma rays could be utilized as the morphological markers for the mutant selection of Heteropappus hispidus (Thunb.).

Induction of micronuclei in human, rabbit and dog lymphocytes irradiated in vitro with gamma radiation (사람, 토끼 및 개 유래 말초혈액 림프구의 미소핵을 이용한 방사선 피폭의 생물학적 선량측정)

  • Ryu, Si-yun;Kang, Bit-na;Kim, Ho-jun;Kim, Tae-hwan;Jeong, Kyu-sik;Kim, Se-ra;Lee, Hae-june;Kim, Sung-ho;An, Mi-Young
    • Korean Journal of Veterinary Research
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    • v.42 no.1
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    • pp.1-6
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    • 2002
  • The frequencies of gamma-ray-induced micronuclei (MN) in cytokinesis-blocked (CB) lymphocytes at several doses were measured in three donors of three species (human, rabbit, dog). Measurements performed after irradiation showed a dose-related increases in MN frequency in each of the donors studied. When analysed by linear-quadratic model the line of best fit was : human : $y=0.1184D+0.01867D^2+0.01$, rabbit : $y=0.0387D+0.00528D^2+0.01$ (y = number of MN/CB cells and D = irradiation dose in Gy). The relative sensitivity of rabbit lymphocytes compared with human lymphocytes was estimated by best fitting linear-quadratic model based on the radiation-induced MN data over the range from 0 Gy to 4 Gy. In the case of MN frequency with 0.2, the relative sensitivities of rabbit lymphocytes was 0.39. These data indicate that the induction of MN in rabbit CB cells following irradiation was much less sensitive to the MN induction effects of gamma-irradiation than those from human. The MN assay with dog lymphocytes was very difficult and time-consumed because the dog PHA-stimulated lymphocytes yielded cultures with very low level of CB cells formation in the condition of this experiment. Our in vitro radiobiological study confirmed that the cytogenetic response obtained in blood from rabbit can be utilized for application in environmental studies.

Effects of gamma-irradiation on intracellular proliferation of Toxoplasma gondii RH tachyzoites (톡소포자충 RH tachyzoites에 대한 감마선 조사가 충체의 숙주세포내 증식에 미치는 영향)

  • 국진아;오성희
    • Parasites, Hosts and Diseases
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    • v.33 no.3
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    • pp.173-178
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    • 1995
  • A quantitative assay was performed on the effects of gamma-irradiation (30- 300 Gy) on intracellular proliferation of Toxoplosmn gonnii RH tachyzoites in human leukemic HL-60 cells and murine peritoneal macrophages by means of 3H-uracil uptake assay. Infected non-irradiation group (NI) and uninfected group (incubating only host cells) were prepared. The 3H-uracil uptake by tachyzoites of NI group 12-24 hrs after infection was 2,190-4,787 counts per minute for macrophages and 2,967-8,254 for HL-60 cells, whereas the irradiated tachyzoites revealed only 381-703 (100 Gy) and 218-408 (300 Gy) for macrophages, and 1,911-2,618 (30 Gy), 1,253-1,384 (70 Gyl, 1,013-1,090 (100 Gyl, and 483-588 (300 Gy) for HL-60 cells. The proliferation inhibition rate was similar in macrophages and HL-60 cells, for example, 89-94% and 80-94% respectively by 300 Gy, 12-24 hrs after infection. It is concluded that RH tachyzoites of T gondii are severely affected by gamma-irradiation in their capability of Intracellular proliferation.

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A comparative study of radioprotection with Callophyllis japonica extract and amifostine against lethal whole body gamma irradiation in mice

  • Shin, Tae-Kyun;Kim, Hee-Chul;Kim, Jeong-Tae;Ahn, Mee-Jung;Moon, Chang-Jong;Hyun, Jin-Won;Jee, Young-Heun;Lee, Nam-Ho;Park, Jae-Woo
    • Advances in Traditional Medicine
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    • v.10 no.1
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    • pp.1-6
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    • 2010
  • The efficacy of the radioprotective effect of Callophyllis japonica ethyl acetate (CJEA) extract was studied by comparing it to that of amifostine, a well-known radioprotective agent, and by evaluating the dose reduction factor, an indicator of radioprotective efficacy. Pretreatment with CJEA extract (100 mg/kg body weight) prior to receiving 12 Gy irradiation significantly improved the survival of jejunal crypts at 3.5 day post-irradiation, but attenuated the level of malondialdehyde compared to vehicle alone (P < 0.01). A similar gastroprotective effect was also obtained in the amifostine-treated irradiated group (P < 0.01). The efficacy of the radioprotective effect was further confirmed by the dose reduction factor, 1.41. Collectively, these results suggest that CJEA extract is a useful radioprotectant whose efficacy is similar to that of amifostine and whose radioprotective mechanism is in part the reduction of lipid peroxidation caused by gamma irradiation.

Effect of Gamma-Irradiation on the Storage Stability and Quality of Polished Wheat (밀쌀의 저장성(貯藏性)과 품질(品質)에 미치는 감마선(線) 조사(照射)의 영향)

  • Lee, Su-Rae;Kim, Sung-Kih;Lee, Kwan-Young
    • Korean Journal of Food Science and Technology
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    • v.5 no.2
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    • pp.95-100
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    • 1973
  • In order to study the effect of gamma-ray on the storage stability and quality of polished wheat, the grain was irradiated at $50{\sim}1000$ krad levels and quality assessment was made for three-month storage period. The results are summarized as follows: 1) Growth of molds was apparent at equilibrium moisture content above 14.8% of the grain, but their growth was retarded at 250 krad level. 2) Irradiation at 250 krad level had no adverse effect on fat acidity, reducing sugar content and color of the grain. 3) Irradiation of the grain caused the increase of degree of gelatinization and the decrease of viscosity. 4) Irradiation at 250 krad level brought about higher digestibility of the grain but had no effect on the rate of retrogradation.

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Effects of Ixeris dentata Extracts on the Genotoxicity Induced by Gamma Irradiation in Rats

  • Kim, Jin Kyu;Woo, Hyun Jung;Kim, Ji Hyang;Yoon, Yang Dal
    • Korean Journal of Environmental Biology
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    • v.22 no.4
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    • pp.543-549
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    • 2004
  • Ixeris dentata is a typical oriental herb. It is a widely distributed perennial in Korea, Japan and China, which belongs to the Compositae Family. The whole plant of I. dentata has been used for the treatment of pneumonia, contusion, tumor and hepatitis. It has also been used for the treatment of allergic diseases as a folk therapy in Korea. I. dentata is known to have aliphatics, triterpenoids and sesquiterpene glycosides in its composition. The present study was designed to explore the protective effects of water- and ethanol- extracts from I. dentata on irradiated rodents. For oral administration (twice per day), the extractive powder of I. dentata and the positive control (ascorbic acid) were dissolved at a concentration of 0.5 and 250mg $ml^{-1}$ in saline, respectively. Thirty days after irradiation, the ratio of the weight of the testis to the body weight was lower than 50% in the radiation groups than the control group. The ALP concentrations in the group treated with the water-extracts of the leaf were $79.68\pm{1.39%}$ (p<0.05) of those of the radiation control. Both of the SGOT and SGPT in the group treated with the ethanol-extract of the root were $72.68\pm{0.95}\;and\;77.87\pm{5.74}$ (p<0.05) of those of the radiation control, respectively. The levels of DNA damage induced by gamma radiation decreased in the experimental group to which the extracts of I. dentata were administered before irradiation. In conclusion, these results indicate that the extracts of I. dentata have an excellent ability to reduce the radicals and they have a protective effect on DNA breakage caused by radiation.

Radiation Preservation of Fishery Products. II. Application of Radiation Preservation to Cure Shrimp, Common Squid and Little Squid

  • Sang Choe;Chung, Tai-Wha;Kwak, Hi-Sang
    • Nuclear Engineering and Technology
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    • v.1 no.1
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    • pp.23-31
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    • 1969
  • Whole cure shrimp, eviscerated common squid and little squid meats were irradiated with gamma radiation from 60Co using varying dose up to one Mrad. Changes in quality were assessed by organoleptic, chemical (VRS and TMA-N), biochemical (hypoxanthine) and microbiological tests; and the effect of radiation on prolonging of the storage life at 2-4 C was studied. With the cure shrimp, 0.12-0.20 Mrad appears to be optimal with 8 days of storage life, whereas the unirradiated control samples had a storage life of 3 days. With the common squid meat, 0.20-0.30 Mrad appears to be optimal with 14-21 days of storage life, where-as the unirradiated control samples had a storage life of 8 days. And for the little squid meat, 0.12-0.20 Mrad appears to be optimal with 10 days of storage, where-as the unirradiated control samples had a storage life of 5 to 6 days.

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