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하지에 인가한 전기 자극이 자세안정성에 미치는 영향 (Effects of Postural Stability using Electrical Stimulation on the Lower Limb)

  • 이선연;유미;김동욱;김남균
    • 대한의용생체공학회:의공학회지
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    • 제30권3호
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    • pp.255-262
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    • 2009
  • The present study analyzed the association between postural control and electrical stimulation by measuring body sway when use the electrical stimulations were applied to different stimulation zones in lower limbs. The subjects were 14 young adults and were, tested for two different visual condition: eyes open and eyes closed. The experiments were also performed in two different stance case: one legged stance and two legged stance while electrical stimulations were applied concurrently or individually to tibialis anterior and triceps surae. Postural responses were assessed by analyzing COP sway path, sum of COP sway measured by a forceplate. The results showed that the direction of the COP shift changed in accordance with the direction of stimulation and showed sensory adaptation as the experiment progressed for two legged stance case. For one legged stance case, concurrent electrical stimulation both sides of muscles was found to be effective for enhancement of postural balance control.

Development of Leuconostoc sp. Host Vector System

  • Eom, Hyun-Ju;Park, Myeong-Soo;Ji, Geun-Eog;Han, Nam-Soo
    • 한국미생물생명공학회:학술대회논문집
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    • 한국미생물생명공학회 2004년도 Annual Meeting BioExibition International Symposium
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    • pp.323-327
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    • 2004
  • Leuconostoc citreum CBUE isolated from kimchi proved to harbor a small cryptic plasmid, pNS75. The complete nucleotide sequence of pNS75 was 1,821 bp and had a low G+C content of 39.2%. Computer analysis using DNASIS revealed one open reading frame (ORF), having ATG as putatitive start condon and potentially encoding proteins with molecular mass of 38 kDa. The chimeric plasmid pLeuCM was first constructed wih pNS75, pUC19 and chroamphenicol acetyltransferase (CAT) from Staphylococcus sp.. pLeuCM replicated and expressed chroamphenicol acetyltransferase in Leuconostoc citerum CBNF after transformation. To test the availability of shuttle vector as cloning vehicle of foreign gene, $\alpha$-amylase gene of Streptococcus bovis was cloned and all transformants secreated the $\alpha$-amylase successfully. The result indicates that pLeuCM is a potential shuttle vector for Leuconostoc spp. and lactic acid bacteria.

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Phage Conversion for β-Lactam Antibiotic Resistance of Staphylococcus aureus from Foods

  • Lee, Young-Duck;Park, Jong-Hyun
    • Journal of Microbiology and Biotechnology
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    • 제26권2호
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    • pp.263-269
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    • 2016
  • Temperate phages have been suggested to carry virulence factors and other lysogenic conversion genes that play important roles in pathogenicity. In this study, phage TEM123 in wild-type Staphylococcus aureus from food sources was analyzed with respect to its morphology, genome sequence, and antibiotic resistance conversion ability. Phage TEM123 from a mitomycin C-induced lysate of S. aureus was isolated from foods. Morphological analysis under a transmission electron microscope revealed that it belonged to the family Siphoviridae. The genome of phage TEM123 consisted of a double-stranded DNA of 43,786 bp with a G+C content of 34.06%. A bioinformatics analysis of the phage genome identified 43 putative open reading frames (ORFs). ORF1 encoded a protein that was nearly identical to the metallo-β-lactamase enzymes that degrade β-lactam antibiotics. After transduction to S. aureus with phage TEM123, the metallo-β-lactamase gene was confirmed in the transductant by PCR and sequencing analyses. In a β-lactam antibiotic susceptibility test, the transductant was more highly resistant to β-lactam antibiotics than S. aureus S133. Phage TEM123 might play a role in the transfer of β-lactam antibiotic resistance determinants in S. aureus. Therefore, we suggest that the prophage of S. aureus with its exotoxin is a risk factor for food safety in the food chain through lateral gene transfer.

LITHOAUTOTROPHIC NITROGEN REMOVAL WITH ANAEROBIC GRANULAR SLUDGE AS SEED BIOMASS AND ITS MICROBIAL COMMUNITY

  • Ahn, Young-Ho;Lee, Jin-Woo;Kim, Hee-Chul;Kwon, Soo-Youl
    • Environmental Engineering Research
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    • 제11권4호
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    • pp.173-180
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    • 2006
  • Autotrophic nitrogen removal and its microbial community from a laboratory scale upflow anaerobic sludge bed reactor were characterized with dynamic behavior of nitrogen removal and sequencing result of molecular technique (DNA extraction, PCR and amplification of 16S rDNA), respectively. In the experiment treating inorganic wastewater, the anaerobic granular sludge from a full-scale UASB reactor treating industrial wastewater was inoculated as seed biomass. The operating results revealed that an addition of hydroxylamine would result in lithoautotrophic ammonium oxidation to nitrite/nitrate, and also hydrazine would play an important role for the success of sustainable nitrogen removal process. Total N and ammonium removal of 48% and 92% was observed, corresponding to nitrogen conversion of 0.023 g N/L-d. The reddish brown-colored granular sludge with a diameter of $1{\sim}2\;mm$ was observed at the lower part of sludge bed. The microbial characterization suggests that an anoxic ammonium oxidizer and an anoxic denitrifying autotrophic nitrifier contribute mainly to the nitrogen removal in the reactor. The results revealed the feasibility on development of high performance lithoautotrophic nitrogen removal process with its microbial granulation.

Examining Early Childhood Education and Care Programs in China

  • Moon, Hyukjun
    • International Journal of Human Ecology
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    • 제17권1호
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    • pp.39-51
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    • 2016
  • This pilot study examined programs for early childhood education and care in three Chinese cities. The samples for this study were one university-based kindergarten, one private kindergarten (rural area), and one public kindergarten (urban area). Six types of instruments were used to assess early childhood education and care services regarding quality matters. Most questions asked were open-ended; consequently, the interview answers by kindergarten directors or teachers (as well as observations made by the researcher) formed the basis for collecting the data. Quality components for each program were determined by a comparison method. The comparison allowed the researcher to understand the range of quality education and care available as well as the variability of existing kindergarten programs in China. The findings of the investigation revealed important information on; (a) demographic information (fees, class hours, total weeks of class, vacation, number of children and staff, class size, and teacher-child ratio) (b) teacher characteristics, (c) health practices, (d) safety practices, (e) work environment, (f) physical settings, (g) play materials, (h) parent involvement, (i) nutrition, (j) daily schedule. Implications within the Chinese context for quality practices and issues to further develop and strengthen early childhood education and care systems are discussed.

SplitScreen: Enabling Efficient, Distributed Malware Detection

  • Cha, Sang-Kil;Moraru, Iulian;Jang, Ji-Yong;Truelove, John;Brumley, David;Andersen, David G.
    • Journal of Communications and Networks
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    • 제13권2호
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    • pp.187-200
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    • 2011
  • We present the design and implementation of a novel anti-malware system called SplitScreen. SplitScreen performs an additional screening step prior to the signature matching phase found in existing approaches. The screening step filters out most non-infected files (90%) and also identifiesmalware signatures that are not of interest (99%). The screening step significantly improves end-to-end performance because safe files are quickly identified and are not processed further, and malware files can subsequently be scanned using only the signatures that are necessary. Our approach naturally leads to a network-based anti-malware solution in which clients only receive signatures they needed, not every malware signature ever created as with current approaches. We have implemented SplitScreen as an extension to ClamAV, the most popular open source anti-malware software. For the current number of signatures, our implementation is $2{\times}$ faster and requires $2{\times}$ less memory than the original ClamAV. These gaps widen as the number of signatures grows.

리튬이차전지용 바나듐계 양극의 제초 특성 (The Preparation Characteristics of Vanadium-based Cathode for Lithium Secondary Battery)

  • 박수길;김종진;;이주성
    • 한국전기전자재료학회:학술대회논문집
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    • 한국전기전자재료학회 1998년도 춘계학술대회 논문집
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    • pp.395-398
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    • 1998
  • Lithium insertion has been studied in a number of vanadium oxides with special regard to their application as the active materials in rechargeable lithium cells. Very high stoichiometric energy densities for lithium insertion are found for several of these materials. Some vanadium oxides, e.g. V$_2$ $O_{5}$ and V$_{6}$ $O_{13}$, are now being used in commercially developed rechargeable Li batteries. Another material which is receiving remarkable attention for this kind of cells is LiV$_3$ $O_{8}$. In variety of ternary lithium-vanadium oxides, the lithium content can be varied between certain limits without major changes in the vanadium oxygen lattice. In our worts, the oxides which do net form these thermodynamically stable bronzes can still accommodate large amounts of lithium at ambient temperature, forming kinetically stable insertion compounds. These compounds owe their existence to the whereas lithium is easily introduced into these open structures. The oxides investigated are rather poor electronic conductors; the conductivity decrease with increase in the lithium content. Improvements in the electrode fabrication technique are needed to alleviate this Problem.oblem.

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인천국제공항 공항전력정보시스템 설계 (Airport Power Information System Design in the Inchon Inta'l Airport)

  • 오영달;민성준;임정규;문정호;이태식;윤태혁;김문호;문영현
    • 대한전기학회:학술대회논문집
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    • 대한전기학회 1998년도 하계학술대회 논문집 C
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    • pp.1121-1124
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    • 1998
  • Inchon International Airport(IIA) is constructed for Northeast Asia Gate as is important for IIA to become a 21 century's leader in the world, as is planned for open at december 2000 as a important economic link for unified korea. Airport Power Information System(APIS) is designed through investigation of advanced domestic and international example. In addition to power facility operation it is designed for using an information infrastructure of whole airport. APIS is newly designed for uninterupted power supply, effective power management, information infrastructure's construction. making a profit by positively introduction of new technology for collection and conservation of power related data, correlation with airport infra systems, data interrelative arrangement about related systems like outdoor lighting system and automatic measuring record, CCTV systems for remote power facility monitoring, and promotion of management ability in the emergency status.

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30kVA 초전도전기 개발(II) ; 설계 및 성능평가 (30kVA Superconducting Generator Development : Design and Performance Test)

  • 백승규;손명환;권영길;고락길;이언용;류강식;박도영;김용주;안종보;김국헌
    • 대한전기학회:학술대회논문집
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    • 대한전기학회 1999년도 하계학술대회 논문집 A
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    • pp.232-234
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    • 1999
  • A 30kVA superconducting generator (S.G.) has been designed, developed and successfully tested in KERI before the end of last year. The design is based on 2-dimensional electromagnetic field analysis of cylindrical coordinate. The field winding of rotor has been wound with superconducting wire of Nb-Ti alloy and tested after assembled with other rotor components. The stator has air-gap type armature windings which allow higher terminal voltage and more sinusoidal voltage waveform than conventional iron cored machines. Steady-state open & short-circuit test and lamp load test have been conducted also. The results of tests are given in this paper and compared with design quantities. Moreover the cooling scheme and characteristics of test system is included.

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인천국제공항 전력시스템 설계 (Power System Design in the Inchon Inta'l Airport)

  • 오영달;민성준;임정규;문정호;이태식;이교승;손주영;김종한;문영현
    • 대한전기학회:학술대회논문집
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    • 대한전기학회 1998년도 하계학술대회 논문집 C
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    • pp.1125-1127
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    • 1998
  • Inchon International Airport(IIA) is constructed for Northeast Asia Gate as is important for IIA to become a 21 century's leader in the world. as is planned for open at December 2000 as a important economic link for unified korea. Power System is designed through investigation of advanced domestic and international example. In addition to power facility operation it is designed for using an information infrastructure of whole airport. IIA Power System Design make instantaneously the Power Distribution Facilities and the SCADA System to construct the airport. and the Airport Power Information System to operate the Power System. It is designed to take efficient and safe Power System including the advanced technology. Power System make the integrated Power Information Database to operate the Main Control Center, analyze the data about the relation of the Power System and Airport operation, and will support the important files in the future.

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