Proceedings of the Korean Society for Applied Microbiology Conference (한국미생물생명공학회:학술대회논문집)

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Development of Leuconostoc sp. Host Vector System

  • Eom, Hyun-Ju (Department of Food Science and Technology, Research Center for Bioresource and Health (RCBH), Chungbuk National University) ;
  • Park, Myeong-Soo (Research Center, BIFIDO Co.) ;
  • Ji, Geun-Eog (Department of Food Science and Nutrition, Seoul National University) ;
  • Han, Nam-Soo (Department of Food Science and Technology, Research Center for Bioresource and Health (RCBH), Chungbuk National University)
  • Published : 2004.06.01
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Abstract

Leuconostoc citreum CBUE isolated from kimchi proved to harbor a small cryptic plasmid, pNS75. The complete nucleotide sequence of pNS75 was 1,821 bp and had a low G+C content of 39.2%. Computer analysis using DNASIS revealed one open reading frame (ORF), having ATG as putatitive start condon and potentially encoding proteins with molecular mass of 38 kDa. The chimeric plasmid pLeuCM was first constructed wih pNS75, pUC19 and chroamphenicol acetyltransferase (CAT) from Staphylococcus sp.. pLeuCM replicated and expressed chroamphenicol acetyltransferase in Leuconostoc citerum CBNF after transformation. To test the availability of shuttle vector as cloning vehicle of foreign gene, $\alpha$-amylase gene of Streptococcus bovis was cloned and all transformants secreated the $\alpha$-amylase successfully. The result indicates that pLeuCM is a potential shuttle vector for Leuconostoc spp. and lactic acid bacteria.

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