• Journal of Microbiology and Biotechnology
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• v.22 no.12
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• pp.1782-1789
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• 2012

We have developed a novel type of polymer nanogel loaded with anticancer drug based on bio-derived poly(${\gamma}$-glutamic acid) (${\gamma}$-PGA). ${\gamma}$-PGA is a highly anionic polymer that is synthesized naturally by microbial species, most prominently in various bacilli, and has been shown to have excellent biocompatibility. Thiolated ${\gamma}$-PGA was synthesized by covalent coupling between the carboxyl groups of ${\gamma}$-PGA and the primary amine group of cysteamine. Doxorubicin (Dox)-loaded ${\gamma}$-PGA nanogels were fabricated using the following steps: (1) an ionic nanocomplex was formed between thiolated ${\gamma}$-PGA as the negative charge component, and Dox as the positive charge component; (2) addition of poly(ethylene glycol) (PEG) induced hydrogen-bond interactions between thiol groups of thiolated ${\gamma}$-PGA and hydroxyl groups of PEG, resulting in the nanocomplex; and (3) disulfide crosslinked ${\gamma}$-PGA nanogels were fabricated by ultrasonication. The average size and surface charge of Dox-loaded disulfide cross-linked ${\gamma}$-PGA nanogels in aqueous solution were $136.3{\pm}37.6$ nm and $-32.5{\pm}5.3$ mV, respectively. The loading amount of Dox was approximately 38.7 ${\mu}g$ per mg of ${\gamma}$-PGA nanogel. The Dox-loaded disulfide cross-linked ${\gamma}$-PGA nanogels showed controlled drug release behavior in the presence of reducing agents, glutathione (GSH) (1-10 mM). Through fluorescence microscopy and FACS, the cellular uptake of ${\gamma}$-PGA nanogels into breast cancer cells (MCF-7) was analyzed. The cytotoxic effect was evaluated using the MTT assay and was determined to be dependent on both the concentration and treatment time of ${\gamma}$-PGA nanogels. The bio-derived ${\gamma}$-PGA nanogels are expected to be a well-designed delivery carrier for controlled drug delivery applications.

• Journal of Microbiology and Biotechnology
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• v.22 no.12
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• pp.1636-1643
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• 2012

Enzymatic pre-bleaching by modification of pulp fibers with xylanases is an attractive approach to reduce the consumption of toxic bleaching chemicals in the paper industry. In this study, an alkaliphilic endoxylanase gene was isolated from metagenomic DNA of a structurally stable thermophilic lignocellulose-degrading microbial consortium using amplification with conserved glycosyl hydrolase family 10 primers and subsequent genome walking. The full-length xylanase showed 78% sequence identity to an endo-${\beta}$-1,4-xylanase of Clostridium phytofermentans and was expressed in a mature form with an N-terminal His6 tag fusion in Escherichia coli. The recombinant xylanase Xyn3F was thermotolerant and alkaliphilic, working optimally at $65-70^{\circ}C$ with an optimal pH at 9-10 and retaining >80% activity at pH 9, $60^{\circ}C$ for 1 h. Xyn3F showed a $V_{max}$ of 2,327 IU/mg and $K_m$ of 3.5 mg/ml on birchwood xylan. Pre-bleaching of industrial eucalyptus pulp with no prior pH adjustment (pH 9) using Xyn3F at 50 IU/g dried pulp led to 4.5-5.1% increase in final pulp brightness and 90.4-102.4% increase in whiteness after a single-step hypochlorite bleaching over the untreated pulp, which allowed at least 20% decrease in hypochlorite consumption to achieve the same final bleaching indices. The alkaliphilic xylanase is promising for application in an environmentally friendly bleaching step of kraft and soda pulps with no requirement for pH adjustment, leading to improved economic feasibility of the process.

• Journal of Korean Society of Environmental Engineers
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• v.35 no.10
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• pp.717-723
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• 2013

The Korea Ministry of Environment prepares some paticulate matter eliminate measures for national health protection, as the Paticulate Matter ($PM_{2.5}$) exceeds the standard at more than half of the monitoring posts installed in the nation's big cities. At the center of JeonJu, when measuring the ultrafine particles of inner car at the different driving conditions, at the condition of the Actuator of inner recirculation mode and the Blower of 2-speed, the reduction speed of the ultrafine particles is most fast and the concentration stays low. When the windows are opened during driving, outer pollutants enter the car and also inner paticulate matter flies in all direction, and the increase of passengers causes the scattering of the ultrafine paticles. As the filter for air cleaning, the using of polypropylene non-woven fabric (used commonly now) is most excellent, but for the removal of volatile organic substance as well as the paticulate matter, it is thought that the using of activated carbon fiber filter, carbon adsorbent, is even more excellent.

• Reproductive and Developmental Biology
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• v.28 no.1
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• pp.1-6
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• 2004

This study was conducted to investigate the effects of donor cell type, individual, passage number and trypsinization time on the in vitro development of bovine somatic cell nuclear transfer embryos. Three cell types (skin, muscle and cumulus cells) and cells from 3 individuals were used for nuclear transfer. Cell were passaged by 5, 15 or 30 times, and cell were trypsinized for 1 or 3 min before injection. Nuclear transfer were performed by conventional fusion method. Development rates to the blastocyst stage were not significantly different among three cell types (16.5∼23.9%) and individuals (16.4∼19.5%). Blastocyst formation rate of cloned embryos reconstituted with cells at passage 30 (5.8%) was significantly lower than those of embryos reconstituted with 5- and 15-passaged cells (25.3 and 23.5%, respectively, P＜0.05). The rate of embryos developed to the blastocyst stage was higher in embryos reconstituted with cells trypsinized for 1 min (30.7%) compared to embryos reconstituted with cells trypsinized for 3 min (P＜0.05). The result of the present study indicates that different donor cell types and individuals used in this study did not affect the development of cloned bovine embryos. However, passage number and trypsinization time of donor cells affect the in vitro development of cloned bovine embryos.

• Proceedings of the Korea Contents Association Conference
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• 2008.05a
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• pp.262-269
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• 2008

This research, to find the cultural identity of taekwondo, presents the historical origin of taekwondo in lights of Choi-centric theory, kwan-centric theory, karate inflow theory, and successive traditional martial arts theory. Then it investigates taekwondo's inner identity as a Korean traditional martial art according to presence, popularity, and independence, three factors of identity suggested by Tak, S. S.(2000).As a result, it reached these conclusions: First, taekwondo was formed after going through fusion of Hong Hi Choi, karate income in period of cultural severance during Japanese occupation, the first kwan leaders and Korean traditional martial arts, process of disorder and cultural reproduction. Second, taekwondo has essentially inherited and developed Korean barehanded martial arts' movements that lay stress on foot skills, and this distinguishes it from hand-skill-based Japan's karate and China's Wushu Furthermore, trunk/groin protectors and headgears are blended remarkably with taeguk patterns and traditional Colors of Five Directions. All terms rendered into pure Korean words shows the essence of Korean localization. It is therefore concluded that Koreans should, along with trying to overcome exclusive nationalism as a country of origin, build a new paradigm of establishing an identity as a global martial art sport.

• Journal of the Society of Disaster Information
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• v.14 no.3
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• pp.325-333
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• 2018

Purpose: There have been terrorist attacks all over the world, causing massive damage. In Korea, there are few cases of terrorist damage, but the lack of the on-site response experience lead to constraints on the opportunity to verify capabilities against terrorism. Therefore, in this study, the chemical terrorism on-site countermeasures is considered as a concept of service, it was possible to draw up improvement alternatives for each element by reviewing at once the interactions between the concerned organizations and citizens in the field and the utilization of the equipment and facilities in the invisible area. Results: It had been evaluated the service process of the counterpart manager from the viewpoint of the victim citizen, who is the beneficiary of the final security service. It has found out the waiting point(W.P) between the victim's civilian incident management, and the element of failure(F.P). Conclusion: In this way, comparing chemical terror incident response manuals belonged to each related organization by fusion of service design approach and scenario technique, It is expected that it will be able to find out more specific problems and to find improvement alternatives.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.18 no.2
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• pp.243-253
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• 2017

This study aims to compare Jeju Island, Shanghai, and Yokohama cruise ports and to investigate the relative strengths and weaknesses of Jeju Island. This study conducted comparative evaluation of 8 factors [airport, berth, expenses, distance, access to town, shopping facilities, source market (background market), and tourism] through a cruise network analysis. Comparative evaluation results find that Jeju Island, which secured 2 berths (berthing capacity) by completion of the civil-military complex port, has ascendancy over Shanghai cruise port and will have ascendancy over Yokohama cruise port once construction of the new airport is completed. Therefore, it will qualify as the best cruise port in Northeast Asia in the future. Results obtained from the cruise network analysis provide insights for administrative policy. Study limitations include only eight factors are used for evaluating the cruise-ship harbor area and surrounding hinterlands, and thus cannot sufficiently evaluate the complex conditions of the sites. In the future, it is necessary to reexamine the evaluation factors of the cruise ship harbor in detail. Furthermore, future research will need to consider the economic effects of the cruise ship industry, and its relevance to related industries as a possible fusion or hybrid industry.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.17 no.5
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• pp.538-547
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• 2016

With respect to the factors affecting information security awareness and behavior, the study of the relevance of the concept of optimistic bias is actively used in psychology. In other words, this study examines whether the optimistic bias of individuals affects information security in the field. In this sense, this study attempted to demonstrate the relevance of optimistic bias in information security behavior and awareness. A questionnaire survey was conducted targeting 111 people engaged in domestic private enterprises. The survey results showed that this personalized optimistic bias exists because of empirical factors related to personal security. Optimistic bias affects the security awareness information. The greater the optimistic bias, the lower the awareness and recognition of information security. In other words, optimistic bias affects information security awareness. Reducing the effects of optimistic bias is expected to reduce information security incidents, such as information leakages. However, the variety of information related ethical activities of a company did not have any effect on the information security awareness. Most previous studies have only examined the effect optimistic bias in the field of health. Therefore, this study fills an important gap in research in IT.

• Cartoon and Animation Studies
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• s.39
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• pp.297-320
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• 2015

Modern technology in the development and convergence of art brought the rapid development of the media arts actively accommodating 'media art' digital media. It has to go to expand the area and limitations as a basis of communication in the 21st century, a new artistic creativity, through the gathering of the existing chain of modern digital mobile technologies and new genre public art that is based on interactive communication break down the boundaries between sectors It has evolved into. This fusion of high technology and the art of mobile interactive media art is to overcome the limitations of time and physical space, as an active subject of interaction and participation, and expanding the range of experiential communications, such as art and science, cultural industries giving provides a flexible platform for a variety of applications. This study presents an expanded paradigm of the new communication and interactive media that define the reporting year review through the literature on art, experiential mobile communications through a case study of mobile interactive media art that is used as a medium of artistic expression the interactive effect was analyzed as a possible new public art. Convergence and interactivity, mobile interactive media art as a buzzword experience has proposed a new approach to high-tech and art and meet the new sensibility of our life and communication, unlimited possibilities worthy of the contemporary trend of convergence and consilience with a new art genre is expected to continued to evolve and develop.

• Journal of Intelligence and Information Systems
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• v.14 no.1
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• pp.35-45
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• 2008

In order to constructan ubiquitous computing environment, it is necessary to develop a technology that can recognize users and circumstances. In this regard, the question of recognizing and expressing user Context regardless of computer and language types has emerged as an important task under the heterogeneous distributed processing system. As a means to solve this task of representing user Context in the ubiquitous environment, this paper proposes to describe user Context as the most similar form of human thinking by using semantic web and fuzzy concept independentof language and computer types. Because the conventional method of representing Context using an usual collection has some limitations in expressing the environment of the real world, this paper has chosen to use Fuzzy OWL language, a fusion of fuzzy concept and standard web ontology language OWL. Accordingly, this paper suggests the following method. First we represent user contacted environmental information with a numerical value and states, and describe it with OWL. After that we transform the converted OWL Context into Fuzzy OWL. As a last step, we prove whether the automatic circumstances are possible in this procedure when we use fuzzy inference engine FiRE. With use the suggested method in this paper, we can describe Context which can be used in the ubiquitous computing environment. This method is more effective in expressing degree and status of the Context due to using fuzzy concept. Moreover, on the basis of the stated Context we can also infer the user contacted status of the environment. It is also possible to enable this system to function automatically in compliance with the inferred state.