• Journal of Korean Neurosurgical Society
• /
• 제50권6호
• /
• pp.528-531
• /
• 2011

Although posterior segmental fixation technique is becoming increasingly popular, surgical treatment of craniovertebral junctional disorders is still challenging because of its complex anatomy and surrounding critical neurovascular structures. Basilar invagination is major pathology of craniovertebral junction that has been a subject of clinical interest because of its various clinical presentations and difficulty of treatment. Most authors recommend a posterior occipitocervical fixation following transoral decompression or posterior decompression and occipitocervical fixation. However, both surgical modalities inadvertently sacrifice C0-1 and C1-2 joint motion. We report two cases of basilar invagination reduced by the vertical distraction between C1-2 facet joint. We reduced the C1-2 joint in an anatomical position and fused the joint with iliac bone graft and C1-2 segmental fixation using the polyaxial screws and rods C-1 lateral mass and the C-2 pedicle.

• Journal of Microbiology and Biotechnology
• /
• 제12권2호
• /
• pp.336-339
• /
• 2002

A total of 100 Corynebacterial clones exerting a regulatory effect on the aceB promoter of Corynebacterium glutamicum were isolated by utilizing a reporter carrying the enteric lacZ gene fused to the promoter. The isolated clones were classified into 3 groups of A, B, and C, according to their color of colonies. Escherichia coli cells carrying clones in groups A and B showed a $90\%\;and\;50\%$ reduction in ${\beta}$-galactosidase activity, respectively. The introduction of group A clones into C. glutamicum also resulted in an almost complete reduction in the expression of the aceA and aceB genes, suggesting that the clones express repressor-like proteins for the genes. Although white colonies were formed on plates containing X-gal, E. coli cells carrying one of the clones in group C exhibited intact ${\beta}$-galactosidase activity. The result suggests that the clone may encode proteins that prevent the cells from accumulating the chromogenic compound, X-gal.

• 한국전기전자재료학회논문지
• /
• 제35권4호
• /
• pp.366-371
• /
• 2022

Recently many efforts have been made to develop a novel class of non-fullerene electron acceptor materials for high-performance organic solar cells. In this work, anthraquinone derivatives, TMAQ and THAQ, were prepared and their availability as electron acceptor materials for organic solar cells were investigated in terms of optical, thermal, electrochemical properties, and solar cell devices. Compared to TMAQ, a significant bathochromic shift of absorption band was observed for THAQ owing to intramolecular hydrogen-bond-assisted CT interactions. Thanks to the fused aromatic ring structure and benzoquinone unit, both TMAQ and THAQ exhibited a high thermal stability and an efficient electron reduction process. In particular, the intramolecular O-H---O=C hydrogen bond of THAQ plays an important role in improving the thermal stability and electron reduction properties. In the P3HT:acceptor solar cell system, THAQ-based devices had more than ca. 6 times higher power conversion efficiency than TMAQ -based devices. These results serve as a guide for developing high-efficient anthraquinone-based electron acceptor materials.

• Mycobiology
• /
• 제51권5호
• /
• pp.273-280
• /
• 2023

The nucleolus is the largest, membrane-less organelle within the nucleus of eukaryotic cell that plays a critical role in rRNA transcription and assembly of ribosomes. Recently, the nucleolus has been shown to be implicated in an array of processes including the formation of signal recognition particles and response to cellular stress. Such diverse functions of nucleolus are mediated by nucleolar proteins. In this study, we characterized a gene coding a putative protein containing a nucleolar localization sequence (NoLS) in the rice blast fungus, Magnaporthe oryzae. Phylogenetic and domain analysis suggested that the protein is orthologous to Rrp8 in Saccharomyces cerevisiae. MoRRP8-GFP (translational fusion of MoRRP8 with green fluorescence protein) co-localizes with a nucleolar marker protein, MoNOP1 fused to red fluorescence protein (RFP), indicating that MoRRP8 is a nucleolar protein. Deletion of the MoRRP8 gene caused a reduction in vegetative growth and impinged largely on asexual sporulation. Although the asexual spores of DMorrp8 were morphologically indistinguishable from those of wild-type, they showed delay in germination and reduction in appressorium formation. Our pathogenicity assay revealed that the MoRRP8 is required for full virulence and growth within host plants. Taken together, these results suggest that nucleolar processes mediated by MoRRP8 is pivotal for fungal development and pathogenesis.

• 대한치과보철학회지
• /
• 제36권1호
• /
• pp.120-132
• /
• 1998

This stusy was to investigate the marginal fitness of porcelain-fused-to- metal crown after succesive firing cycle. Main variables were the degree of marginal curvature of labiocervical margin and the type of alloy. The exaggerated marginal curvature(EMC) was created by additional reduction at the faciocervical wall of the normallized marginal curvature (NMC)-typed ivorine tooth by using milling machine. The difference in the shape was the mid facial margin was placed 2mm apical to cemento- enamel junction in labial surface. Three types of alloy were high noble, noble, and base metal alloy. Test specimens were divided into 8 groups and each group had 8 specimens. Sixty four ceramometal crowns were made totally. Measurement stages were following degassing, opaquing. body porcelain firing, and glazing, and measuring sites were 4. (midmesial, midfacial, middistal, and midlingual). Digital, travelling measuring microscope (0.5 um precision, Olympus. Japan) was used under ${\times}250$ magnification. Within the limitation of this investigation, it was concluded as belows: 1. The pattern of marginal distortion was varied. Degassing stage was not a specific, causative stage that induce most of total marginal distortion during whole procedure fabricating a ceramometal crown. Body firing stage induced discrepancy relatively more than other firing stages. 2. The specimens that were Ni-based alloy and had EMC were distorted persistently following successive fabricating procedures. But marginal openings were decreased after glazing. 3. The release of metal grinding-induced stress was presumed as a cause that induce marginal distortion. 4. The amount of discrepancies of the labial and lingual margins were greater than that of the mesial and distal margin in the specimen that had EMC. 5. Silver-plated die was not enough to resist abrasion during repeated seating of metal copings on the die-holding device.

• 전기학회논문지
• /
• 제66권8호
• /
• pp.1265-1271
• /
• 2017

In this paper, the speech parameter fusion method is studied to improve the performance of the conventional emotion recognition system. For this purpose, the combination of the parameters that show the best performance by combining the cepstrum parameters and the various pitch parameters used in the conventional emotion recognition system are selected. Various pitch parameters were generated using numerical and statistical methods using pitch of speech. Performance evaluation was performed on the emotion recognition system using Gaussian mixture model(GMM) to select the pitch parameters that showed the best performance in combination with cepstrum parameters. As a parameter selection method, sequential feature selection method was used. In the experiment to distinguish the four emotions of normal, joy, sadness and angry, fifteen of the total 56 pitch parameters were selected and showed the best recognition performance when fused with cepstrum and delta cepstrum coefficients. This is a 48.9% reduction in the error of emotion recognition system using only pitch parameters.

• 반도체디스플레이기술학회지
• /
• 제15권2호
• /
• pp.1-5
• /
• 2016

3D printer is the equipment of the system for sequentially layer laminated in the materials. Now 3D printer used in various fields such as, semiconductor, electricity automobile, medical and various types of output method and material. In this paper, we studied about the improvement on warpage due to shrinkage of product from 3D printer of FDM(Fused Deposition Modeling) type, we proposed measures systematically to solve warpage problem using of 6SC(6 Step Creativity) method of practical TRIZ. After experimented with product prototypes experiment, we verified effect about solution.

• 한국결정성장학회지
• /
• 제25권6호
• /
• pp.257-262
• /
• 2015

현재 전 세계적으로 지구온난화의 주범인 $CO_2$ 저감을 위한 노력을 하고 있으며, 산업발전에 필요한 화력발전 등에서 발생한 부산물을 재활용하는 방안이 시급하다. 따라서 본 연구는 산업부산물인 비산재와 폐기물 슬래그를 이용하여 모르타르 경화체를 제작하였으며 알칼리 활성화제 첨가에 따른 물성을 비교하기 위해 비교적 낮은 농도인 3 M의 NaOH solution을 사용하였고, 이 경화체에 초임계 이산화탄소 조건($40^{\circ}C$, $80kgf/cm^2$ pressure, 60 min)에서 탄산화를 통하여 $CO_2$를 경화 체내에 안정적으로 고정화 시켜 이에 따른 물성을 평가하였다. 탄산화 인자인 CaO의 함량이 많을수록 탄산화율은 높게 나타났으며, 탄산화 후 무게변화율이 최대 약 12 % 증가하였다. 탄산화 후 압축강도는 전과 비슷한 수준이었으며, 이를 통해 탄산화를 통해 $CO_2$를 안정하게 고정화시킨 친환경 소재에 적용 가능성을 확인할 수 있었다. 또한 탄산화 반응 후에 생성되는 $H_2O$로 인해 추가적인 양생을 통해 장기적인 관점에서 탄산화를 통해 물성향상 또한 기대해 볼 수 있을 것으로 생각된다.

• 복식
• /
• 제61권1호
• /
• pp.58-68
• /
• 2011

With Expo 2012 Yeosu to be held in 2012, this study intends to propose designs for fashion cultural products into which the characteristics of local cultures of Yeosu are fused. Using symbols of Expo 2012 Yeosu and cultural symbols of Yeosu City as motifs, this study will develop patterns and then, apply them to neckties, handkerchiefs, and T-shirts. Adobe Illustrator CS2 and Adobe Photoshop CS2 will be used. This study developed basic motifs so that cultural resources using camellia, Odong Island, and the Turtle Ship, which represent Yeosu City, could be well harmonized with the topic of Expo 2012 Yeosu. This paper set three basic motifs of new formative images, using graphic images that were made by omission of forms, simplification, overlap, repetition, and calligraphy of the name of Yeosu holding the event. Each set motif was expanded to three motifs again through change, conversion, and mixture of colors, and three types of repetitive applied patterns were developed through revolution, symmetry, repetition, and reversal of each motif. The modern and refined image for neckties, to which the developed motif was applied, was made by directly applying the repetitive pattern of each motif or by making $45^{\circ}$ revolution. For handkerchiefs, revolution, enlargement, reduction, and gradation were applied to the motif so the pattern could be highlighted to the maximum and finally, a colorful image was developed. For T-shirts, three designs-a half-sleeved box type, a sleeveless round neckline type, and a sleeveless V-neckline tight type-were developed, through which availability of the design was increased. Through enlargement, reduction, revolution, and repetition of each motif, this paper layout the pattern on the whole part of a T-shirt, pursuing a decorative and casual image.

• Molecules and Cells
• /
• 제26권6호
• /
• pp.611-615
• /
• 2008

DNA methylation is an epigenetic mechanism for gene silencing. In Arabidopsis, MET1 is the primary DNA methyltransferase that maintains CG DNA methylation. Plants having an overall reduction of MET1 activity, caused by a met1 mutation or a constitutively expressed MET1 antisense gene, display genome hypomethylation, inappropriate gene and transposon transcription, and developmental abnormalities. However, the effect of a transient reduction in MET1 activity caused by inhibiting MET1 expression in a restricted set of cells is not known. For this reason, we generated transgenic plants with a MET1 antisense gene fused to the DEMETER (DME) promoter (DME:MET1 a/s). Here we show that DME is expressed in leaf primordia, lateral root primoridia, in the region distal to the primary root apical meristem, which are regions that include proliferating cells. Endogenous MET1 expression was normal in organs where the DME:MET1 a/s was not expressed. Although DME promoter is active only in a small set of cells, these plants displayed global developmental abnormalities. Moreover, centromeric repeats were hypomethylated. The developmental defects were accumulated by the generations. Thus, not maintaining CG methylation in a small population of proliferating cells flanking the meristems causes global developmental and epigenetic abnormalities that cannot be rescued by restoring MET1 activity. These results suggest that during plant development there is little or no short-term molecular memory for reestablishing certain patterns of CG methylation that are maintained by MET1. Thus, continuous MET1 activity in dividing cells is essential for proper patterns of CG DNA methylation and development.