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Expression Analysis of Anthocyanin Biosynthetic Genes of Tassel and Silks in Gwangpyeongok and Dacheongok (광평옥과 다청옥의 수이삭과 수염에서 안토시아닌 생합성 유전자 발현 분석)

  • Go, Young Sam;Bae, Hwan Hee;Choi, Yu Chan;Son, Jae Han;Ha, Jun Young;Shin, Seong Hyu;Jung, Tae Wook
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.66 no.3
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    • pp.240-247
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    • 2021
  • Anthocyanins are known to be involved in various functions such as antioxidant and antibacterial activities in plants. Although studies on anthocyanins in corn have been conducted recently, basic research related to anthocyanin biosynthesis is insufficient. In this study, we examined the molecular biological and physicochemical properties related to anthocyanin biosynthesis in the tassel and silks of Gwangpyeongok and Dacheongok cultivars. Anthocyanins were not synthesized in either the tassel or silks in Gwangpyeongok, whereas were synthesized in both in Dacheongok. The total anthocyanin content was approximately 30 times higher in the tassel and silks of Dacheongok than in those of Gwangpyeongok. In addition, C-3-G was measured only in the tassel of Dacheongok, and C-3-G, Pg-3-G, and M-3-G were 45.2 times, 27.3 times, and 37.6 times higher, respectively, in the silks of Dacheongok than of Gwangpyeongok. Expression of F3'H, DFR, and GST genes decreased in the tassel, and that of F3'H and DFR genes decreased in the silks of Gwangpyeongok. It was further confirmed that transcription factor P1 and R1 regulate the expression of anthocyanin biosynthetic genes in the tassel and silks, respectively, in Gwangpyeongok. Linoleic acid (C18:2) decreased by 6.6% and 10.9%, and linolenic acid (C18:3) increased by 8.5% and 8.5%, in the tassel and silks, respectively, of Gwangpyeongok compared to those of Dacheongok. Palmitic acid (C16:0) increased by 4.1% and oleic acid (C18:1) decreased by 2.1% in the silks of Gwangpyeongok compared to that in Dacheongok. In addition, the total fatty acid content in the tassel and silks increased by 10.3% and 30.4%, respectively, in Gwangpyeongok compared to that in Dacheongok. However, no significant results were observed in the analysis of phytosterol components. These results may be utilized as useful resources for the development of functional corn containing a large amount of anthocyanins.

An Analysis of Social Perception on Forest Using News Big Data (뉴스 빅데이터를 활용한 산림에 대한 사회적 인식 변화 분석)

  • Jang, Youn-Sun;Lee, Ju-Eun;Na, So-Yeon;Lee, Jeong-Hee;Seo, Jeong-Weon
    • Journal of Korean Society of Forest Science
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    • v.110 no.3
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    • pp.462-477
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    • 2021
  • The purpose of this study was to understand changes in domestic forest policy and social perception of forests from a macro perspective using big data analysis of news articles and editorials. A total of 13,570 'forest' related data were collected from metropolitan and economic journals from 1946-2017 using keyword and CONCOR (Convergence of iterated Correlations) analysis. First, we found the percentage of articles and editorials using the keyword 'forest'increased overall. Second, news data on 'forest' in the field of reporting was concentrated in the "social" sector during the first period (1946-1966), followed by forest-related issues expanding to various fields from the second (1967-1972) to fifth (1988-1997) periods, then toward the "culture" sector in the sixth (1998-2007) and "politics" after the seventh (2008-2017) period. Third, we found changes in the policy paradigm over time significantly changed social awareness. In the first and second periods, people experienced livelihood issues rather than forest greening or forest protection policy and expanded their awareness of planned and scientific afforestation (third) to environmental protection (fourth) and ecological perspectives (sixth to seventh). The key outcome of our analysis was leveraging news big data that reflected polices on forests and public social perception To further derive future social issues,more in-depth analysis of public discourse and perception will be possible using textual big data and GDP of various social network services (SNS), such as combining blogs and YouTube.

Distribution and Natural Regeneration of Abies holophylla in Plantations in Gapyeong, Gyeonggi-do (경기도 가평 지역 조림지 내 전나무(Abies holophylla)의 분포와 천연갱신)

  • Nam, Kwanghyun;Joo, Kwang Young;Choi, Eun Ho;Jung, Jong Bin;Park, Pil Sun
    • Journal of Korean Society of Forest Science
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    • v.110 no.3
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    • pp.341-354
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    • 2021
  • A large part of Gapyeong is occupied by Korean pine (Pinus koraiensis) and Japanese larch (Larix kaempferi) plantations. Abies holophylla stands are scattered throughout Gapyeong, but little information on their distribution is available. This study explored the potential of succession from planted species to native A. holophylla in plantations. Trees were inventoried and regeneration of A. holoplhylla and stand management history were examined in Korean pine, Japanese larch, and A. holophylla-dominated stands. The importance percentage of A. holophylla was the highest among species with a range of 36.1% to 79.1% in all stands and the density of A. holophylla in understory (DBH <2 cm or <1.3 m height) ranged from 50 to 5,820 trees ha-1. Non-metric multidimensional scaling classified stands into four types, AN, AP, AM, and P. The AN type showed a reverse J-shape DBH distribution, which was similar to that in natural A. holophylla stands. Both AP and AM types included Korean pine plantations with A. holophylla seed trees within stands. For AP, A. holophylla competed with planted species in overstory and deciduous broadleaved species in understory. The AM type was once thinned from below, thus stem density in the mid DBH classes was lower than upper or lower DBH classes. The P type consisted of plantations without A. holophylla seed trees. However, understory regeneration of A. holophylla was abundant through seed supply from A. holophylla in adjacent stands. Plantations with A. holophylla seed trees within or in adjacent stands showed vigorous natural regeneration of A. holophylla, highlighting the potential for succession from planted species to native A. holophylla in the Gapyeong area. Further studies can help develop techniques to restore plantations to native species-dominated natural stands using ecological succession.

Trend in Paternal Childcare Time for Preschool Children in Korea from 2004 to 2019 (아버지의 미취학자녀 돌봄시간 변화 추이 분석(2004-2019))

  • Lee, Jung-eun;Seo, Jiwon
    • Journal of Family Resource Management and Policy Review
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    • v.25 no.3
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    • pp.103-120
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    • 2021
  • Recently, the importance of the fathers role in the care of young children has been emphasized in Korea for the balance of childcare responsibilities between mothers and fathers. This study investigates the trends in paternal childcare in Korea over the last 15 years. Childcare is divided into primary and developmental care and fathers's Childcare time and participation rates are inverstigated for dual- and single-income households. Data are collected from the four waves of the five-yearly Statistics of Korea Life Time Surveys between 2004(t1) and 2019(t4) including the workday time diaries of fathers with preschool children(n1=2,264, n2=1,242, n3=959, n4=952). Three major results are identified. First, paternal childcare time and participation rates have increased with dual-income fathers spending 24 more minutes a day with their young child(ren) in 2019 than in 2004, which is nearly double. Second, in the analysis of fathers' childcare time use and participation rates comparing primary and developmental care, primary care is found to have increased more than developmental care, especially among dual income fathers: this further exhibits a reversed relation between primary and developmental care over time. Third, the determinants of paternal childcare time are fathers' age, market labor time, commuting time, gender equality consciousness, and education. In particular, market labor time was significant in all four waves, while gender equality consciousness is only significant for single-income fathers. Based on these results, a specific agenda is provided for family-friendly policies to improve the balance of childcare roles between fathers and mothers, especially encouraging increased(significant and sufficient) participation of fathers in primary care activities.

Emodin Studies on Anti-inflammatory and Skin Barrier Improvement Activities (Emodin의 항염 및 피부장벽개선 활성 연구)

  • Kim, Se-Gie;Choi, Jae Gurn;Jang, Young-Ah
    • Journal of the Korean Applied Science and Technology
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    • v.38 no.6
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    • pp.1383-1392
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    • 2021
  • It has been reported that emodin, a major pharmacologically active ingredient of herbal medicines such as Polygonum cuspidatum, Polygonum multiflorum, Rheum palmatum, and Aloe vera, is effective in antioxidant, antibacterial, anti-inflammatory, anticancer, and liver protection. In this study, to investigate the potential of emodin to be used as a skin disease and functional material, the activity related to the improvement of inflammation and skin barrier function was confirmed. To observe the anti-inflammatory effect on HaCaT cells, which are human keratinocytes, cytokine inhibition was confirmed by ELISA kit and protein expression by western blot. In HaCaT cells activated with TNF-α (10 ng/mL)/IFN-γ (10 ng/mL), emodin was treated with each concentration (5, 10, 20, 40) µM. As a result, It was confirmed that the production amount of TNF-α, IL-1β and IL-6 decreased as the concentration of emodin increased. In the experimental results on the expression levels of inflammation-related proteins iNOS and COX-2, it was confirmed that 48% of iNOS and 29% of COX-2 were inhibited compared to control at a concentration of 20 µM of emodin. As an indicator of skin barrier function improvement, the mRNA expression level of filaggrin, involucrin, and loricirn and the production amount of filaggrin, involucrin, and loricirn were confirmed. and excellent results were obtained with an emodin concentration-dependent increase. In particular, filaggrin, which was produced twice as much as the control at a concentration of 20 µM, is a protein involved in the formation of NMF, a natural moisturizing factor, and is known to play an important role in moisturizing the stratum corneum. In conclusion, it was confirmed that emodin can be used as a material for improving inflammation and improving skin barrier function, which is part of the potential for use as a skin disease and functional material. It is believed that if additional research is performed in the future, the scope of its application can be further expanded.

Estimation of Ground-level PM10 and PM2.5 Concentrations Using Boosting-based Machine Learning from Satellite and Numerical Weather Prediction Data (부스팅 기반 기계학습기법을 이용한 지상 미세먼지 농도 산출)

  • Park, Seohui;Kim, Miae;Im, Jungho
    • Korean Journal of Remote Sensing
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    • v.37 no.2
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    • pp.321-335
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    • 2021
  • Particulate matter (PM10 and PM2.5 with a diameter less than 10 and 2.5 ㎛, respectively) can be absorbed by the human body and adversely affect human health. Although most of the PM monitoring are based on ground-based observations, they are limited to point-based measurement sites, which leads to uncertainty in PM estimation for regions without observation sites. It is possible to overcome their spatial limitation by using satellite data. In this study, we developed machine learning-based retrieval algorithm for ground-level PM10 and PM2.5 concentrations using aerosol parameters from Geostationary Ocean Color Imager (GOCI) satellite and various meteorological parameters from a numerical weather prediction model during January to December of 2019. Gradient Boosted Regression Trees (GBRT) and Light Gradient Boosting Machine (LightGBM) were used to estimate PM concentrations. The model performances were examined for two types of feature sets-all input parameters (Feature set 1) and a subset of input parameters without meteorological and land-cover parameters (Feature set 2). Both models showed higher accuracy (about 10 % higher in R2) by using the Feature set 1 than the Feature set 2. The GBRT model using Feature set 1 was chosen as the final model for further analysis(PM10: R2 = 0.82, nRMSE = 34.9 %, PM2.5: R2 = 0.75, nRMSE = 35.6 %). The spatial distribution of the seasonal and annual-averaged PM concentrations was similar with in-situ observations, except for the northeastern part of China with bright surface reflectance. Their spatial distribution and seasonal changes were well matched with in-situ measurements.

Effect of 17β-estradiol on Ecdysteroid Pathway Related Genes in the Brackish Water Flea Diaphanosoma celebensis (17β-estradiol이 기수산 물벼룩의 Ecdysteroid 경로에 미치는 영향)

  • In, Soyeon;Yoo, Jewon;Cho, Hayoung;Lee, Young-Mi
    • Journal of Marine Life Science
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    • v.5 no.2
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    • pp.35-42
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    • 2020
  • 17β-estradiol (E2) is a natural hormone secreted by ovary, and continuously discharged from household and livestock wastewater into aquatic environment. Due to its strong estrogenic activity, it has adverse effects on development and reproduction in crustacean as an endocrine disrupting chemical. Although ecdysteroid signaling pathway play a key role in development in crustacean, little information on transcriptional modulation of ecdysteroid-related genes in response to E2 is available in small crustacean. Here, we investigated the acute toxicity of E2 to obtain 24-h LCx values in the brackish water flea Diaphanosoma celebensis. Time-dependent expression patterns of seven ecdysteroid pathway - related genes (CYP314a1, EcRA, EcRB, USP, ERR, Vtg, VtgR) were further examined using quantitative real time reverse transcriptase polymerase chain reaction (qRT-PCR). As results, 24-h LC50 and LC10 values were 9.581 mg/l and 4.842 mg/l, respectively. The mRNA expression of CYP314a1, EcRA, USP, VtgR was significantly up-regulated at 12 or 24 h after exposure to E2. These findings indicate that E2 can affect their molting and reproduction by modulating the expression of ecdysteroid pathway - related in D. celebensis. This study will be useful for better understanding of molecular mode of action of endocrine disrupting chemicals on molting process in small crustacean.

Antibacterial Effect on Enterococcus Faecalis and Physical Properties of Chitosan Added Calcium Hydroxide Canal Filling Material (키토산 첨가 수산화칼슘 근관 충전재의 Enterococcus Faecalis에 대한 항균 효과 및 물리적 성질)

  • Song, Sol;Kim, Yu-Jin;Lee, Jung-Hwan;Lee, Joonhaeng;Shin, Jisun;Kim, Jongbin
    • Journal of the korean academy of Pediatric Dentistry
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    • v.48 no.2
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    • pp.198-208
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    • 2021
  • The aim of this study was to evaluate the antibacterial effect on Enterococcus Faecalis and physical properties of chitosan added calcium hydroxide canal filling material. Low, medium, high molecular weights of chitosan powder were mixed with calcium hydroxide canal filling material. Also, for each molecular weight group, 1.0, 2.0, 5.0 wt% of chitosan powder were added. An overnight culture of E. faecalis was adjusted to 1 × 106 CFU/ml. For test of antibacterial effect, three different molecular weights of 2.0 wt% chitosan and three different concentrations of high molecular weight chitosan were mixed with calcium hydroxide canal filling material. The absorbance of plates was analyzed using spectrophotometer at 570 nm with a reference wavelength of 600 nm. Physical properties such as flow, film thickness and radiopacity were examined according to ISO 6876 : 2012. All molecular weight type of chitosan containing material showed inhibitory effect against E. faecalis growth compared to non-chitosan added calcium hydroxide canal filling material group (p < 0.05). High molecular weight chitosan containing material showed the most antibacterial effect. Also, the antibacterial effect decreased as the incorporated amount of chitosan decreased (p < 0.05). Every molecular weight group of material containing chitosan had a tendency for reduced flow and radiopacity, increased film thickness according to amount of chitosan. Low molecular weight of 1.0 wt% chitosan addition did not show any significant difference of physical properties compared to conventional calcium hydroxide canal filling material. In conclusion, for reinforcement of antibacterial effect against E. faecalis and for favorable physical properties, 2.0 wt% of chitosan adding is recommended. Considering its antibacterial effect of chitosan, further studies are required for clinical application of chitosan in endodontics and pediatric dentistry.

Analysis of Optimal Pathways for Terrestrial LiDAR Scanning for the Establishment of Digital Inventory of Forest Resources (디지털 산림자원정보 구축을 위한 최적의 지상LiDAR 스캔 경로 분석)

  • Ko, Chi-Ung;Yim, Jong-Su;Kim, Dong-Geun;Kang, Jin-Taek
    • Korean Journal of Remote Sensing
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    • v.37 no.2
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    • pp.245-256
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    • 2021
  • This study was conducted to identify the applicability of a LiDAR sensor to forest resources inventories by comparing data on a tree's position, height, and DBH obtained by the sensor with those by existing forest inventory methods, for the tree species of Criptomeria japonica in Jeolmul forest in Jeju, South Korea. To this end, a backpack personal LiDAR (Greenvalley International, Model D50) was employed. To facilitate the process of the data collection, patterns of collecting the data by the sensor were divided into seven ones, considering the density of sample plots and the work efficiency. Then, the accuracy of estimating the variables of each tree was assessed. The amount of time spent on acquiring and processing the data by each method was compared to evaluate the efficiency. The findings showed that the rate of detecting standing trees by the LiDAR was 100%. Also, the high statistical accuracy was observed in both Pattern 5 (DBH: RMSE 1.07 cm, Bias -0.79 cm, Height: RMSE 0.95 m, Bias -3.2 m), and Pattern 7 (DBH: RMSE 1.18 cm, Bias -0.82 cm, Height: RMSE 1.13 m, Bias -2.62 m), compared to the results drawn in the typical inventory manner. Concerning the time issue, 115 to 135 minutes per 1ha were taken to process the data by utilizing the LiDAR, while 375 to 1,115 spent in the existing way, proving the higher efficiency of the device. It can thus be concluded that using a backpack personal LiDAR helps increase efficiency in conducting a forest resources inventory in an planted coniferous forest with understory vegetation, implying a need for further research in a variety of forests.

Recognition and Narrative Aspects of the History of Korean Classic Literature from Two Korean Literature History Works Written in China (중국 한국문학사 2종의 한국고전문학사 인식과 서술 양상: 남북한문학사와 자국문학사의 수용과 변용을 중심으로)

  • Lee, Deung-yearn
    • Cross-Cultural Studies
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    • v.48
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    • pp.67-106
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    • 2017
  • This study focuses on two specific history of Korean literature in Chinese: the outline of The History of Joseon Literature (2010) by Li Yan and The History of Joseon Literature (1988, 2008) by Wei Xu-sheng; it was conducted to compare narrative viewpoints to the history of South and North Korean literature and therefore identify distinguishable characteristics. As a result, the following was concluded. First, The History of Korean Literature by Cho Dong-il and The History of Korean Literature in North Korea (15 volumes) include thorough discussions on division of historical eras, concept of genres as well as individual literary works and applied such discussions on writing literary history. However, Wei Xu-sheng and Li Yan's The History of Korean Literature did not illuminate theoretical discussion of South and North Korea. Li Yan's outline of The History of Joseon Literature was published in 2010 and the first edition of Wei Xu-sheng's The History of Joseon Literature was published in 1986 and later was published as revised editions in 2000 and 2008. Regarding published dates, it is a matter of course to reference Cho Dong-il's The History of Korean Literature, published in the 1980s, or The History of Korean Literature in North Korea (15 volumes), published in the 1990s; nevertheless, neither Wei Xu-sheng nor Li Yan used those texts in their works. Their works were heavily influenced by the narrative tradition of the history of national literature and therefore, entailed unsophisticated discussion on the division of historical eras or the concept of genres. Second, those two texts also emphasized external factors such as politics, society, economy and culture and explicitly mention these factors in historical overview of each chapter. Such an approach is commonly used in narratives of literary history under socialist regimes, including The History of Korean Literature in North Korea (15 volumes). Accordingly, evaluations based on 'political standards' - stress of people, nationality, practicality and so forth - in main texts are particularly accentuated, akin to narratives of literary history under socialist regimes. Finally, since those two Korean literature history works are written by Chinese scholars, they focus on correlation between Chinese literature history and Korean literature history. However, several genre-related terminologies such as Xiaopin (a kind of essay), Yuefu (a kind of popular song/poem), Yuyan (fable), Shuochang (telling of popular stories with the interspersal songs), Shizhuan (biography or/and memoirs in history) were adopted directly from Chinese literature. In analyzing Korean literature using terminologies introduced from Chinese literature, differences between original and alternative definitions were not examined in detail. While some terminologies and concepts were adopted directly without further consideration as to state of the two nations, it is also interesting to note that dichotomy, mainly used in Korean literature history, was used to discuss the genre of Cheonki (romance tale), relevant to Suyichon and Keumosinhua, rather than follow traditions of Chinese literature history.