• The Plant Pathology Journal
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• v.35 no.5
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• pp.417-424
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• 2019

Melanose, caused by Diaporthe citri, is one of severe diseases in citrus, a major economic resource in Jeju island. To reduce the usage amount of organic synthetic fungicide, bio-sulfur was tested as an alternative chemical to control citrus melanose in the present study. Direct antifungal activity of bio-sulfur against D. citri was determined through in vitro experiment using artificial nutrient media. Disease severity of melanose on bio-sulfur pretreated citrus leaves was lower than that on untreated ones. To illustrate the mechanism of disease suppression by bio-sulfur, infection structures were observed with a fluorescent microscope and a scanning electron microscope. In fluorescent microscopic observation, most conidia rarely germinated. In addition, hyphal growth on leaves pretreated with bio-sulfur was inhibited compared to that on untreated ones. In scanning electron microscope images of bio-sulfur pretreated leaves, surfaces of most conidia were shrunk while hyphae were morphologically changed and frequently branched. Such microscopic observations were also found for leaves pretreated with a commercial fungicide Dithianon. These results suggest that bio-sulfur may be used to control citrus melanose as an environment friendly alternative to organic synthetic fungicides

• The Korean Journal of Mycology
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• v.41 no.4
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• pp.295-300
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• 2013

This study was conducted to test effect of fungicides on the spore germination and mycelial growth of BotaniGard$^{(R)}$(Beauveria bassiana GHA). Eight fungicides, which are using for plant disease control in cucumber cultivation, with different concentration were mixed with conidia of GHA isolate for 0.5, 2, and 4 hours. The fungicides trifluminazol, fenarimol, ethaboxam, copper hydroxide and tetraconazol did significantly not inhibit on spore germination and mycelial growth. The trifluminazol, propineb and tetraconazol on high concentrations inhibited the mycelial growth of GHA. However, mixing time of fungicide with conidia of GHA did not affect in spore germination and mycelial growth. These results suggest that fungicide effect needed be checked before mycopesticide is applied in the field.

• Research in Plant Disease
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• v.15 no.1
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• pp.17-22
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• 2009

To reduce the amount of chemical fungicides and the number of spray for the control of Alternaria blight of ginseng, biofungicides were used in order to reduce the residue and ensure the safety of ginseng plants. The control efficiencies were 68.34%$\sim$73.56% against Alternaria blight in 2 times alternate spray of biofungicides and chemical fungicide at 14 days interval whereas the control efficiencies were 87.00%$\sim$89.42% in 2 times alternate spray of 3 different kinds of chemical fungicides at 14 days interval. In case of treatment combination the control efficiencies were 74.53%$\sim$87.23% in alternate spray of mixture of biofungicides and chemical fungicides at 28 days interval in all combinations except 2 kinds of combinations (Com-12 and Com-17). Therefore, the alternate application of the biofungicides and chemical fungicides or alternate application of mixture of biofungicides and chemical fungicides could reduce the amount of chemical fungicide about 75.00%$\sim$83.33%.

• The Plant Pathology Journal
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• v.38 no.6
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• pp.629-636
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• 2022

Colletotrichum species is known as the major causal pathogen of red pepper anthracnose in Korea and various groups of fungicides are registered for the management of the disease. However, the consistent use of fungicides has resulted in the development of resistance in many red pepper-growing areas of Korea. Effective management of the occurrence of fungicide resistance depends on constant monitoring and early detection. Thus, in this study, various methods such as agar dilution method (ADM), gene sequencing, allele-specific polymerase chain reaction (PCR), and polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) were applied for the detection of benzimidazole resistance among 24 isolates of Colletotrichum acutatum s. lat. and Colletotrichum gloeosporioides s. lat. The result of the ADM showed that C. gloeosporioides s. lat. was classified into sensitive and resistant isolates to benomyl while C. acutatum s. lat. was insensitive at ≥1 ㎍/ml of benomyl. The sequence analysis of the β-tubulin gene showed the presence of a single nucleotide mutation at the 198th amino acid position of five isolates (16CACY14, 16CAYY19, 15HN5, 15KJ1, and 16CAYY7) of C. gloeosporioides s. lat. Allele-specific PCR and PCR-RFLP were used to detect point mutation at 198th amino acid position and this was done within a day unlike ADM which usually takes more than one week and thus saving time and resources that are essential in the fungicide resistance management in the field. Therefore, the molecular techniques established in this study can warrant early detection of benzimidazole fungicide resistance for the adoption of management strategies that can prevent yield losses among farmers.

• Proceedings of the Korean Society of Plant Pathology Conference
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• 2003.10a
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• pp.87.3-88
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• 2003

TiO$_2$photocatalytic ozonation was attempted to disinfect fungal pathogens causing postharvest spoilage of kiwifruits and to decompose fungicide residuals on kiwifruits. TiO$_2$Photocatalytic ozonation process synergistically degraded organic compound and inhibited conidial germination of the fungal pathogen compared to single treatment of ozonation or photocatalysis. The efficient control of fungal spoilage and degradation of residual fungicide on kiwifruits indicate that TiO$_2$photocatalytic ozonation is a very attractive method for postharvest disease control of kiwifruits as an alternative to fungicides application.

• Proceedings of the Korean Society of Plant Pathology Conference
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• 1998.06a
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• pp.50-69
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• 1998

Dimethomorph is a novel fungicide with a high level of activity against diseases induced by certain Oomycetes, including fungal populations that are resistant to other products. In several ways, this fungicide illustrates the opportunities and challenges presented by many modern pesticides. The specific mode of action, which affects cell wall formation, is associated with a very high level of performance and low dose rates under field conditions. These low dose rates, combined with a low level of toxicity to non-target organisms present an outstanding safety profile. This same highly-specific mode of action, however, limits the spectrum of activity and suggests the need for a resistance management plan, both of which must be addressed in new product development. In addition, the biological and physiochemical properties of this, and other new products are not adequately described by the traditional classification of fungicides into“protectant”and“systemic”types. These unique profiles provide novel and useful products for disease control.

• Korean Journal Plant Pathology
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• v.10 no.4
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• pp.292-296
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• 1994

Main tobacco diseases were surveyed on tobacco plants in the northern Kyeongbuk area to evaluate the effectiveness of current disease control techniques from 1993 to 1994. The major disease in seedlings was the anthracnose (Colletotrichum tabacum) but the mosaic (TMV) in adult plants. Wild fire (Psedomonas syringae pv. tabaci) and hollow stalk (Erwinia carotovora subsp. carotovora) were more severe in 1993 than those in 1994 because of more rain. Establishment of drain-outlet at the bottom of the temporary transplanting pot gave a significant reduction in anthracnose severity. Most farmers have ignored the protection effect of using milk during transplanting and the removal of diseased roots from the fields on mosaic (TMV). It was considered that the educating program on disease diagnosis and fungicide application might help farmers for the proper use of chemicals.

• Research in Plant Disease
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• v.17 no.3
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• pp.295-301
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• 2011

The effect of various fungicides on anthracnose of strawberry, caused by Colletotrichum gloeosporioides, was determined in vitro, and in greenhouse and field trials. The $EC_{50}$ values of benomyl were clearly different between two sensitive and resistant groups of isolates. Iminoctadine tris had lower $EC_{50}$ values than mancozeb and propineb as protective fungicides and the response of mancozeb, propineb and azoxystrobin was variable depending on the isolate. In the greenhouse, pre- and post- inoculation fungicide applications significantly reduced disease compared to the non-treated control. Propineb, mancozeb and azoxystrobin were effective in controlling the disease when applied prior to inoculation. Metconazole and prochloraz-Mn treatments as ergosterol biosynthesis-inhibiting fungicides had the lowest incidence of anthracnose. In the nursery field trials in 2009 and 2010, the reduced fungicide spray program provided similar levels of disease control compared to the calendar-based applications with captan. A reduced spray program based on efficacious fungicides such as prochloraz-Mn will be useful for strawberry growers and provide more options for controlling anthracnose in Korea.

• Journal of Ginseng Research
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• v.26 no.1
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• pp.10-16
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• 2002

In replicated field trials, the efficacy of pentachloronitrobenzene (PCNB; quintozene) for control of damping-off of ginseng seedlings was found to be affected by timing of application and formulation. Application at the time of seeding and prior to placement of straw mulch was found to provide the moat consistent level of disease control. However, decline in plant stand during the four-year production cycle resulted in most treatments providing similar levels of plant populations at harvest. Soil residues of pentachloronitrobenzene were generally highest (1 $\mu\textrm{g}$ PCNB/g soil) in those treatments that exhibited the highest levels of disease control in the seedling year. Straw contained high levels of quintozene after application. Beet seed assays with artificially-infested soils indicated that current use rates provide an amount of product suitable for high levels of disease control.

• Korean Journal of Agricultural and Forest Meteorology
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• v.13 no.1
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• pp.10-19
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• 2011

In order to predict the risk of anthracnose on hot pepper in the future, the projected climate data from SRES A1B scenario in South Korea were used with the modified anthracnose model to calculate Infection Risk (IR), which was to estimate the number of fungicide sprays. Based on daily temperature and precipitation, the anthracnose model resulted in an empirical relationship that IR = (Daily temperature - $16^{\circ}C$) ${\times}$ 0.07 + (Daily precipitation ${\times}$ 0.11). For 135 locations in South Korea, the total number of fungicide sprays needed from 2011 to 2100 was 12,150, indicating a complicated change with an overall increase in anthracnose development in all locations until 2100. In particular, radical changes in anthracnose development were predicted at Yeongdeok, Yeongyang, and Uiseong, whereas gradual changes were predicted at Heongsung, Hamyang and Taean. The eastern counties of Gyeongbuk Province, which ar the major plantation area in these days, would be the place with the highest disease pressure in the future. In addition, the years of 2058, 61, 78 and 2096 will be most severe, requiring 8-11 times of fungicide spraying. The GIS maps show that the mountain areas of Jeonbuk and Chungbuk Province would have the least disease pressure of anthracnose in the future.