• Mycobiology
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• 제32권4호
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• pp.186-189
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• 2004

The study on the cultural characteristics of Bipolaris spicifera was conducted to provide with information for the identification, and inoculation studies, etc. B. spicifera grew well at $30{\sim}35^{\circ}C$ and wide range of pH $5.0{\sim}9.0$. However, the fungal growth was retarded at pH 4.0 and 10.0, respectively. Conidia were germinated with 70% at $30^{\circ}C$ but maintained 50% germination even at $40^{\circ}C$, indicating that this pathogen could infect plants at relatively high temperature. The pathogen could not produce conidia under 24 hr fluorescent light condition for 7 days. In contrast, it produced many more conidia at 24 hr dark condition.

• 농업생명과학연구
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• 제50권5호
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• pp.251-255
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• 2016

In year of 2013, anthracnose disease symptoms were observed on stem of stonecrop in a plant resource field, Southern Forest Resource Research Center, Jinju, South Korea. The disease symptoms were appeared 2-5 cm smudged irregular black spot color on the plant stems. Suspected pathogen was isolated from the lesion with surface sterilization method and identified it as Colletotrichum gloeosporioides by morphological characteristics and phylogenetic analyses. Artificially inoculated the isolated pathogen led to the similar disease symptom as originally observed in the field. The fungal pathogen was re-isolated from the inoculated lesion and it exhibited the same characteristics compared to the original isolate. This is the first report of anthracnose of stonecrop grown in open fields, caused by Colletotrichum gloeosporioides in Korea.

• 농약과학회지
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• 제13권4호
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• pp.332-335
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• 2009

유기농 인증을 받아서 3년 이상이 경과한 농가의 토양을 수집하여 근권 세균을 450균주 분리하였고, 여러 가지 식물 병원균의 균사 생장을 억제하는 세균 KB-401 균주를 선발하였다. KB-401 균주는 특히 감귤 검은점무늬병균(Diaporthe citri)의 균사 생장과 포자 발아를 억제하며, 발아하는 포자에 처리하였을 때 발아관의 선단이 팽윤되는 현상을 관찰할 수 있었다. 본 균주는 생리적인 특성과 16S rDNA의 염기서열을 분석한 결과, Bacillus subtilis로 동정되었다.

• 식물병연구
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• 제24권2호
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• pp.87-98
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• 2018

곰팡이 종들은 유전체내에 대략 10% 정도의 다양한 전이인자와 함께 반복적인 염기서열을 갖는다. 이러한 전이인자들의 대부분은 유전체내에서 활발히 전이되며 곰팡이 병원균의 기주 범위와도 연관성을 갖으며 분포하는 것으로 알려져있다. 화본과 작물에 병을 일으키는 도열병에 분포하는 전이인자들은 활발히 전이하는 것으로 보이며, 특정 기주에 감염하는 개체군에 특이적으로 분포하는 경우가 많았다. 다수의 연구 보고에서도열병균의 전이인자가 비병원성 유전자의 기능을 상실하는데 작용하여, 이로인해 저항성 품종에 병을 일으켰다. 따라서, 도열병균의 전이인자들은 식물-곰팡이 사이의 상호 진화를 유도하는 원동력 중 하나일 수 있다. 본 총설에서는 도열병균에 존재하는 전이인자들의 종류와 생물학적인 기능에 관해 정리하였다.

• The Plant Pathology Journal
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• 제18권5호
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• pp.284-287
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• 2002

The antifungal activity of super reductive water (SRW) against plant pathogenic fungi was examined to extend its application to integrated pest management (IPM) for plant diseases. Diluted solutions ($\times$1/10, $\times$1/25, and $\times$1/50) of SRW inhibited fungal growth of kiwifruit soft rot pathogen, Diaporthe actinidiae, in a concentration dependent manner, When kiwifruits were inoculated on wounds with mycelium blocks, stock and diluted solutions successfully inhibited the disease development. In addition to the high pH of the SRW, fungistatic activity was also considered as the cause of the antifungal effect against the pathogen. Whereas conidial germination of Magnaporthe grisea was not affected by the diluted SRW solutions, appressorium formation was significantly inhibited in a concentration dependent manner, With little harmfulness to human health and environment SRW could be used to control plant pathogenic fungi, particularly appressorium-forming fungal pathogens.

• 한국잡초학회지
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• 제14권3호
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• pp.192-198
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• 1994

잡초병원균 Drechslera portulacae가 생산하는 식물독소를 단리하여 그 화학구조를 결정한 결과, zeaenol으로서 동정하였다. Zeaenol은 기지 물질이나 지금까지 입체화학적인 면에서는 불명인 채 남아있었으며, 본 연구에서 처음으로 그 입체구조를 명백하게 하였다. 또한, 잡초방제적인 측면에서 식물독소의 활용가능성을 찾기 위하여 zeaenol의 제초활성을 조사하였다. 그 결과, 식용피 및 어저귀의 유묘의 초장에 대한 억제효과는 보여주지 않았으나, $3{\times}10^{-5}M$의 처리구에서 식용피 및 어저귀의 유근의 생장을 대조구에 비해 각각 22.8%, 54.8% 억제하였다.

• The Plant Pathology Journal
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• 제35권2호
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• pp.156-163
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• 2019

Our study investigated the available chlorine content, contact time and difference among strains of each pathogen for sodium hypochlorite (NaOCl) to control chemically against soil-borne fungal pathogens, such as Phytophthora rot by Phytophthora cactorum, violet root rot by Helicobasidium mompa, and white root rot by Rosellinia necatrix, causing die-back symptom on apple trees. As a result, the colony growth of Phytophthora cactorum was inhibited completely by soaking over 5 s in 31.25 ml/l available chlorine content of NaOCl. Those of H. mompa and R. necatrix were inhibited entirely by soaking over 160 s in 62.5 and 125 ml/l available chlorine content in NaOCl, respectively. Also, inhibition effect on available chlorine in NaOCl among strains of each soil-borne pathogen showed no significant difference and was similar to or better than that of fungicides.

• The Plant Pathology Journal
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• 제15권3호
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• pp.144-151
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• 1999

Chitinase activities from Shumard oak tissues were determined to study changes in chitinase activities related to water stress. The enzyme extracted in sodium acetate buffer (0.1M, pH 4.5) was assayed by a colorimetric method. In addition, the fungal hyphae of Hypoxylon atropunctatum in xylem tissues of oak were observed through scanning electron microscopy. The enzyme in oak tissues was mainly endochitinase, and optimum pH for enzyme activity was 5. Specific chitinase activities from both of stems held under high relative humidity (ranges of 0.63-1.11 pKatal/$\mu\textrm{g}$ of protein) and stems held under low relative humidity (ranges of 0.41-0.99 pKatal/$\mu\textrm{g}$ of protein) were significantly increased following fungal inoculation with H. atropunctatum. However, there was no significant difference in chitinase activities between tissues held under high and low humidities, which might be due to fungal chitinase. Scanning electron microscopy showed holes in fungal hyphae in the xylem tissues of stems held under high humidity but not in the stems held under ow humidity, suggesting that hyphae might be hydrolyzed by plant hydolases such as chitinase.

• The Plant Pathology Journal
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• 제19권2호
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• pp.85-91
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• 2003

Pre-treatment with DL-3-aminobutyric acid (BABA) in the cucumber plants caused the decrease of disease severity after inoculation with anthracnose pathogen Colletotrichum orbiculare. In this study, ultrastructures of the vascular bundle and the infection structures in the leaves of BABA-treated as well as untreated cucumber plants were observed after inoculation with the anthracnose pathogen by electron microscopy. The ultrastructures of vascular bundle in the leaves of BABA-treated plants were similar to those of the untreated plants except plasmodesmata. In the BABA-treated plants, the plasmodesmata were more numerous than in the untreated plants, suggesting that the BABA treatment may cause the active transfer of metabolites through the vascular bundle. In the leaves of untreated plants, the fungal hyphae were spread widely in the plant tissues at 5 days after pathogen inoculation. Most cellular organelles in the hyphae were intact, indicating a compatible interaction between the plant and the parasite. In contrast, in the leaves of BABA pre-treated plants the growth of most hyphae was restricted to the epidermal cell layer at 5 days after inoculation. Most hyphae cytoplasm and nucleoplasm was electron dense or the intracellular organelles were degenerated. The cell walls of some plant cells became thick at the site adjacent to the intercellular hyphae, indicating a mechanical defense reaction of the plant cells against the fungal attack. Furthermore, hypersensitive reaction (HR) of the epidermal cells was often observed, in which the intracellular hyphae were degenerated. Based on these results it is suggested that BABA causes the enhancement of defense mechanisms in the cucumber plants such as cell wall apposition or HR against the invasion of C. orbiculare.

• The Plant Pathology Journal
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• 제28권4호
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• pp.364-372
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• 2012

Xanthomonas oryzae pv. oryzae causing bacterial leaf blight disease in rice produces and secretes Hpa1 protein that belongs to harpin protein family. Previously it was reported that Hpa1 induced defense responses when it was produced in tobacco. In this study, we expressed hpa1 gene in rice and Arabidopsis to examine the effects of Hpa1 expression on disease resistance to both fungal and bacterial pathogens. Expression of hpa1 gene in rice enhanced disease resistance to both X. oryzae pv. oryzae and Magnaporthe grisea. Interestingly, individual transgenic rice plants could be divided into four groups, depending on responses to both pathogens. hpa1 expression in Arabidopsis also enhanced disease resistance to both Botrytis cineria and Xanthomonas campestris pv. campestris. To examine genes that are up-regulated in the transgenic rice plants after inoculation with X. oryzae pv. oryzae, known defense-related genes were assessed, and also microarray analysis with the Rice 5 K DNA chip was performed. Interestingly, expression of OsACS1 gene, which was found as the gene that showed the highest induction, was induced earlier and stronger than that in the wild type plant. These results indicate that hpa1 expression in the diverse plant species, including monocot and dicot, can enhance disease resistance to both fungal and bacterial plant pathogens.