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http://dx.doi.org/10.5423/PPJ.OA.09.2012.0136

Expression of hpa1 Gene Encoding a Bacterial Harpin Protein in Xanthomonas oryzae pv. oryzae Enhances Disease Resistance to Both Fungal and Bacterial Pathogens in Rice and Arabidopsis  

Choi, Min-Seon (Department of Horticultural Biotechnology and Institute of Life Sciences & Resources, Kyung Hee University)
Heu, Sunggi (Microbial Safety Division, National Academy of Agricultural Science, Rural Development Administration)
Paek, Nam-Chon (Department of Plant Science, Seoul National University)
Koh, Hee-Jong (Department of Plant Science, Seoul National University)
Lee, Jung-Sook (Genomics Division, National Academy of Agricultural Science, Rural Development Administration)
Oh, Chang-Sik (Department of Horticultural Biotechnology and Institute of Life Sciences & Resources, Kyung Hee University)
Publication Information
The Plant Pathology Journal / v.28, no.4, 2012 , pp. 364-372 More about this Journal
Abstract
Xanthomonas oryzae pv. oryzae causing bacterial leaf blight disease in rice produces and secretes Hpa1 protein that belongs to harpin protein family. Previously it was reported that Hpa1 induced defense responses when it was produced in tobacco. In this study, we expressed hpa1 gene in rice and Arabidopsis to examine the effects of Hpa1 expression on disease resistance to both fungal and bacterial pathogens. Expression of hpa1 gene in rice enhanced disease resistance to both X. oryzae pv. oryzae and Magnaporthe grisea. Interestingly, individual transgenic rice plants could be divided into four groups, depending on responses to both pathogens. hpa1 expression in Arabidopsis also enhanced disease resistance to both Botrytis cineria and Xanthomonas campestris pv. campestris. To examine genes that are up-regulated in the transgenic rice plants after inoculation with X. oryzae pv. oryzae, known defense-related genes were assessed, and also microarray analysis with the Rice 5 K DNA chip was performed. Interestingly, expression of OsACS1 gene, which was found as the gene that showed the highest induction, was induced earlier and stronger than that in the wild type plant. These results indicate that hpa1 expression in the diverse plant species, including monocot and dicot, can enhance disease resistance to both fungal and bacterial plant pathogens.
Keywords
bacterial leaf blight pathogen; defense response; harpin; OsACS1 gene; rice blast pathogen;
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