• 한국생물공학회:학술대회논문집
• /
• 한국생물공학회 2001년도 추계학술발표대회
• /
• pp.471-474
• /
• 2001

강한 제초활성을 띠는 곰팡이 균주 YK101의 배양조건을 최적화하기 위하여 균사와 균체 생산에 대한 온도, 교반속도 그리고 배양배지의 영향을 조사하였다. YK101의 최적 배양온도와 교반속도는 각각 $28^{\circ}C$, 150rpm 이었다. 최적화된 배지조성은 1.0$^{\sim}$2.0% total sugar, 1.0% yeast extract 그리고 0.05% $MgSO_4$ ${\cdot}$ $7H_2O$이었다.

• 한국식물병리학회지
• /
• 제11권4호
• /
• pp.380-382
• /
• 1995

Yellow spot or blotch symptoms on the upper surface of leaf, without the production of velvet-like fungi on the lower surface of leaf as in the gray mold of tomato caused by Cladosporium fulvum, were observed in tomato (cv. Seokwang) plants in May, 1995, in a vinyl-house of the experimental plot of Gyeongnam Provincial Rural Development Administration, Chinju, Gyeongnam, Korea. We identified this disease as powdery mildew of tomato caused by Oidiopsis taurica (L v.) Arnaud (=Leveillula taurica), which was new to Korea. Conidia of the fungus were borne on uni- or bi-septated conidiophores which were developed through the stomata of the tomato leaf. The conidia were slender, clavate and variable in size (31~111.6$\times$13.1 ${\mu}{\textrm}{m}$). The fungal conidia isolated from tomato leaves were inoculated to tomato plants, and the occurrence of the same disease was confirmed based on the symptomatology and the morphology of the pathogen reisolated.

• 대한수의학회지
• /
• 제47권3호
• /
• pp.321-324
• /
• 2007

Pneumocystis (P.) carinii is an opportunistic fungal pathogen of many animal species and human, which can cause fatal pneumonia in immunocompromised individuals. Three 100-day-old pigs with progressive atrophy, anorexia and respiratory distress were submitted to the Cheju National University for diagnosis. Grossly, the lungs were enlarged with rubbery consistency. Histopathologically, the lungs were characterized by diffuse interstitial pneumonia with thickening of alveolar septa due to infiltration of macrophages and lymphocytes. Alveolar lumens were filled with a foamy eosinophilic proteinaceous material in which numerous punctiform organisms. The organisms were demonstrated as P. carinii by Grocott-methenamine-silver staining and immunohistochemistry in lungs of two pigs. In our best knowledge, this is believed to be the first report of P. carinii pneumonia in pigs in Korea.

• 미생물과산업
• /
• 제20권1호
• /
• pp.50-52
• /
• 1994

Coccidioides immitis의 mycelial phase와 tissue phase에 있어서 미세구조를 다음과 같은 결론을 얻었다. 1. mycelial phase의 세포구조에서는 정상적인 균이 보유하고 있는 내용물질을 관찰할 수 있었으며 이는 다른 eucaryotic cell의 구조와 같았다. 2. tissue phase에서는 mycelial phase의 세포의 크기보다 비교적 크기가 컸으며 mycelial phase에서 보다 많은 종의 vacuole이 관찰되었다. 3. Tissue phase에서는 fibril form의 내연층을 볼 수 있었다.

• 한국어병학회지
• /
• 제4권2호
• /
• pp.95-100
• /
• 1991

Saprolegnia diclina, which was the pathogen causing death in snake fishes(Channa argus) at culture farm, was investigated using scanning electron microscope. It was found that Saprolegnia diclina infection caused snake fishes to fail gas change in the gills. Cell lysis as well as edematous disease and hyperplasia as a result of Saprolegnea diclina attachment on the surface of gills were observed. The granules, the mean diameters of which ranged from 6 to $7\;{\mu}m$, attaching on the surface of gills were found to be secondary zoospores of Saprolegnia diclina. The failures of gas exchange in the gill cells and circulation as a result of the osmotic dilution of the blood were supposed to be the main cause of death.

• 미생물학회지
• /
• 제9권4호
• /
• pp.169-174
• /
• 1971

Ultrastructural observations of mycelial and tissue phase with dimorphic fungal pathogen Coccidioides immitis were studied by electron microscopy of thin sections. 1. In mycelial phase of C.immitis contains normal cell components such as nucleus, mitochondria, endoplamsic reticulum, intracytoplasmic membrane system, cell wall and cell membrane as observed in the other encaryotic cells. 2. In tissue phase of C. immitis was larger than mycelial phase in cell size and observed much more vacuoles than mycelial phase. 3. In the contrast of mycelial phase of C. immitis, the tissue phase of cells were observed fibril form of capsular layer.

• BMB Reports
• /
• 제38권1호
• /
• pp.82-88
• /
• 2005

Bacillus cereus 28-9 is a chitinolytic bacterium isolated from lily plant in Taiwan. This bacterium exhibited biocontrol potential on Botrytis leaf blight of lily as demonstrated by a detached leaf assay and dual culture assay. At least two chitinases (ChiCW and ChiCH) were excreted by B. cereus 28-9. The ChiCW-encoding gene was cloned and moderately expressed in Escherichia coli DH5$\alpha$. Near homogenous ChiCW was obtained from the periplasmic fraction of E. coli cells harboring chiCW by a purification procedure. An in vitro assay showed that the purified ChiCW had inhibitory activity on conidial germination of Botrytis elliptica, a major fungal pathogen of lily leaf blight.

• The Plant Pathology Journal
• /
• 제20권4호
• /
• pp.264-270
• /
• 2004

Fusarium oxysporum f. sp. fragariae is a fungal pathogen causing strawberry wilt disease. The random amplified polymorphic DNA (RAPD) and restriction fragment length polymorphisms (RFLPs) of intergenic spacer (IGS) region of rDNA were used to identify genetic variation among 22 F. oxysporum f. sp. fragariae isolates. All isolates could be distinguished from each other by RAPD analysis and RFLP of 2.6 kb amplified with primer CNS1 and CNL12 for IGS region of rDNA. Cluster analysis using UPGMA showed eight distinct clusters based on the banding patterns obtained from RAPD and rDNA RFLP. These results indicate that F. oxysporum f. sp. fragariae isolates are genetically distinct from each other, There was a high level genetic variation among F. oxysporum f. sp. fragariae.

• Journal of Microbiology and Biotechnology
• /
• 제25권11호
• /
• pp.1782-1786
• /
• 2015

A new improved PCR method has been developed for the rapid, reliable, and sensitive detection of Venturia nashicola, a destructive pathogen of scab disease in Japanese pear. The translation elongation factor-1 alpha gene-derived PCR primers specifically amplified a 257-bp-sized DNA band of the target gene from the genomic DNA of V. nashicola. No amplicon was produced from the genomic DNA of other Venturia spp. and reference fungal species tested. With the high detection limit of 10 fg DNA content, our real-time method could be used for the quarantine inspection and field monitoring of V. nashicola.

• Journal of Plant Biotechnology
• /
• 제38권4호
• /
• pp.278-284
• /
• 2011

The CCR4-CAF1-NOT complex-mediated degradation of mRNA is a fundamental aspect of gene regulation in eukaryotes. We herein examined the role of AtCAF1 in the innate immune and wound responses of plants. Our results showed that overexpression of AtCAF1 significantly downregulated the transcript level of EFR but not FLS2 and BRI1, as well as abolished up-regulated expression pattern of EFR in response to wounding. Consistently, Agrobacteriummediated transient expression of GUS was highly enhanced in the transgenic plants overexpressing AtCAF. Furthermore, JA responsive genes were down-regulated by overexpression of AtCAF, causing the transgenic plants overexpressing AtCAF more susceptible to necrotrophic fungal pathogen, Botrytis cinerea. These results suggest that The CCR4-CAF1-NOT complex-mediated degradation of mRNA negatively regulates wounding-mediated disease resistance in Arabidopsis thaliana.