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http://dx.doi.org/10.4014/jmb.1507.07095

Specific and Sensitive Detection of the Pear Scab Fungus Venturia nashicola by SYBR Green Real-Time PCR  

Yun, Yeo Hong (Department of Microbiology and Institute of Biodiversity, Dankook University)
Yoon, Seong Kwon (Department of Microbiology and Institute of Biodiversity, Dankook University)
Jung, Jae Sung (Department of Biology, Sunchon National University)
Kim, Seong Hwan (Department of Microbiology and Institute of Biodiversity, Dankook University)
Publication Information
Journal of Microbiology and Biotechnology / v.25, no.11, 2015 , pp. 1782-1786 More about this Journal
Abstract
A new improved PCR method has been developed for the rapid, reliable, and sensitive detection of Venturia nashicola, a destructive pathogen of scab disease in Japanese pear. The translation elongation factor-1 alpha gene-derived PCR primers specifically amplified a 257-bp-sized DNA band of the target gene from the genomic DNA of V. nashicola. No amplicon was produced from the genomic DNA of other Venturia spp. and reference fungal species tested. With the high detection limit of 10 fg DNA content, our real-time method could be used for the quarantine inspection and field monitoring of V. nashicola.
Keywords
Pear scab; real-time PCR; translation elongation factor-1 alpha gene; Venturia nashicola;
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Times Cited By KSCI : 1  (Citation Analysis)
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