• Journal of Microbiology and Biotechnology
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• 제27권2호
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• pp.335-341
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• 2017

The complexity of the bacterial recombination system is a barrier for the construction of bacterial mutants for the further functional investigation of specific genes. Several protocols have been developed to inactivate genes from the genus Pseudomonas. Those protocols are complicated and time-consuming and mostly do not enable easy construction of multiple knock-ins/outs. The current study describes a single and double crossover-recombination system using an optimized vector-free allele-exchange protocol for gene disruption and gene replacement in a single species of the family Pseudomonadaceae. The protocol is based on self-ligation (circularization) for the DNA cassette which has been obtained by overlapping polymerase chain reaction (Fusion-PCR), and carries an antibiotic resistance cassette flanked by homologous internal regions of the target locus. To establish the reproducibility of the approach, three different chromosomal genes (ncRNA31, rpoN, rpoS) were knocked-out from the root-associative bacterium Pseudomonas stutzeri A1501. The results showed that the P. stutzeri A1501 mutants, which are free of any plasmid backbone, could be obtained via a single or double crossover recombination. In order to optimize this protocol, three key factors that were found to have great effect on the efficiency of the homologous recombination were further investigated. Moreover, the modified protocol does not require further cloning steps, and it enables the construction of multiple gene knock-in/out mutants sequentially. This work provides a simple and rapid mutagenesis strategy for genome editing in P. stutzeri, which may also be applicable for other gram-negative bacteria.

• Journal of Microbiology and Biotechnology
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• 제27권7호
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• pp.1266-1271
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• 2017

Lactobacillus fermentum strain JDFM216, isolated from a Korean infant feces sample, possesses the ability to enhance the longevity and immune response of a Caenorhabditis elegans host. To explore the characteristics of strain JDFM216 at the genetic level, we performed whole-genome sequencing using the PacBio system. The circular draft genome has a total length of 2,076,427 bp and a total of 2,682 encoding sequences were identified. Five phylogenetically featured genes possibly related to the longevity and immune response of the host were identified in L. fermentum strain JDFM216. These genes encode UDP-N-acetylglucosamine 1-carboxyvinyltransferase (E.C. 2.5.1.7), ErfK/YbiS/YcfS/YnhG family protein, site-specific recombinase XerD, homocysteine S-methyltransferase (E.C. 2.1.1.10), and aspartate-ammonia ligase (E.C. 6.3.1.1), which are involved in peptidoglycan synthesis and amino acid metabolism in the gut environment. Our findings on the genetic background of L. fermentum strain JDFM216 and its potential candidate genes for host longevity and immune response provide new insight for the application of this strain in the food industry as newly isolated functional probiotic.

• Journal of Microbiology and Biotechnology
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• 제28권8호
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• pp.1247-1259
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• 2018

Raspberries are polyphenol-rich fruits with the potential to reduce the severity of the clinical signs associated with obesity, a phenomenon that may be related to changes in the gut microbiota. The aim of this study was to investigate the effect of raspberry supplementation on the fecal microbiota using an in vivo model of obesity. Obese diabetic db/db mice were used in this study and assigned to two experimental groups (with and without raspberry supplementation). Fecal samples were collected at the end of the supplementation period (8 weeks) and used for bacterial 16S rRNA gene profiling using a MiSeq instrument (Illumina). QIIME 1.8 was used to analyze the 16S data. Raspberry supplementation was associated with an increased abundance of Lachnospiraceae (p = 0.009), a very important group for gut health, and decreased abundances of Lactobacillus, Odoribacter, and the fiber degrader S24-7 family as well as unknown groups of Bacteroidales and Enterobacteriaceae (p < 0.05). These changes were enough to clearly differentiate bacterial communities accordingly to treatment, based on the analysis of UniFrac distance metrics. However, a predictive approach of functional profiles showed no difference between the treatment groups. Fecal metabolomic analysis provided critical information regarding the raspberry-supplemented group, whose relatively higher phytosterol concentrations may be relevant for the host health, considering the proven health benefits of these phytochemicals. Further studies are needed to investigate whether the observed differences in microbial communities (e.g., Lachnospiraceae) or metabolites relate to clinically significant differences that can prompt the use of raspberry extracts to help patients with obesity.

• 정보관리학회지
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• 제28권3호
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• pp.83-101
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• 2011

국제 표준인 ISO 15489와 관련코드에 따르면 공공조직이든 민간조직이든지를 막론하고 안정적인 기록물 분류체계를 구현하게 하는 기능 분류 사용을 권고하고 있다. 기업에서도 이를 따라 업무 수행 기록물을 분류 축적하여 검색 활용할 수 있는 체계를 구축하는 것은 기업 성장을 위해서 중요하다. 따라서 기업의 기록물 분류체계 개발에 적용할 수 있는 분류기준이나 개발 방법론 연구 및 모형의 연구가 반드시 필요하다. 본 연구에서는 우리나라 대기업 3개 회사와 중소기업 4개 회사 등 총 7개의 종합건설기업의 기록물 분류체계 사례연구를 통하여 분류체계의 내용을 비교 분석하였다. 사례연구를 통하여 도출한 분류원칙을 정리 종합하여 핵심적인 건설기록물 분류기준을 제시하고, 건설기록물 분류체계 모형을 도출하기 위하여 대기업 사례기업의 본사조직 및 프로젝트조직의 대분류 및 중분류 항목 구성을 상호 비교하면서 표준적인 본사조직 및 프로젝트조직 기록물 분류 항목을 도출하는 과정을 기술하고 그 결과로 개발된 건설기록물 분류체계 모형을 제시하였다.

• 한국식품영양과학회:학술대회논문집
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• 한국식품영양과학회 2001년도 International Symposium on Food,Nutrition and Health for 21st Century
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• pp.38-47
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• 2001

L-theanine is a unique amino acid, found almost solely in tea plants. It is the main component responsible for the exotic taste of green tea. In our studies of L-theanine, we have found a variety of biological activities including relaxation and the alleviation of PMS. In general, animals generate very weak electric pulses on the surface of the brain, called brain waves. Brain waves are classified into four types, namely $\alpha$, $\beta$, $\delta$ and $\theta$-waves, based on their frequency. Brain waves correlate with individual mental conditions. For example, generation of $\alpha$-waves is considered an index of relaxation. In human volunteers, $\alpha$-waves were generated on the occipital and parietal regions of the brain surface within 40 minutes after the oral administration of 50 or 200 mg Suntheanine$^{TM}$ without causing drowsiness. Premenstrual Syndrome (PMS) is a symptom unique to women which appears in the luteal phase from the ovulation period through the first day of menstruation. It possesses characteristics of having a peak just prior to menstruation and disappearing 1 - 2 days following the start of menstruation. Symptoms of PMS are generally categorized as mental, physical and social symptoms. When comparing the reported Symptoms of PMS by the methods of MDQ score, the Suntheanine$^{TM}$ group was found to have a lower incidence of PMS symptoms, including physical, mental and social symptoms. Overall, a significant alleviation of PMS symptoms by the administration of 200 mg Suntheanine$^{TM}$ was observed. With the successful industrial production of L-theanine, we are now able to supply Suntheanine$^{TM}$, offering a tremendous opportunity for designing functional foods targeting relaxation and the alleviation of PMS.

• Journal of Ginseng Research
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• 제35권1호
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• pp.1-11
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• 2011

Korean ginseng is a medicinally important perennial herb from the family Araliaceae. It has been cultivated for its highly valued medicinal properties for over 1,000 years in east Asian countries such as China, Korea, and Japan. Due to its longtime cultivation in shady areas, ginseng is frequently exposed to pathogenic infections. Plants protect themselves from microbial pathogens using an array of defense mechanisms, some of which are constitutively active, while others are activated upon pathogen invasion. These induced defense responses, controlled by defense-related genes, require tradeoffs in terms of plant fitness. We hypothesize that ginseng, as with other plants, possesses regulatory mechanisms that coordinate the activation of attacker-specific defenses in order to minimize fitness costs while attaining optimal resistance. Several classes of defense-related genes are induced by infection, wounds, irradiation, and other abiotic stresses. Both salicylates and jasmonates have been shown to cause such responses, although their specific roles and interactions in signaling and development are not fully understood in ginseng. This review summarizes possible defense-related genes in ginseng based on their expression patterns against biotic and abiotic stresses and describes their functional roles.

• 혜화의학회지
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• 제13권2호
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• pp.169-178
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• 2004

Now many sustitution and false articles is used in korea instead of mudanpi. To use mudanpi correctly, we will make a quilitative certificational plan of mudanpi to investigate all of lieraturea, records and documents. And we could reach conclusions as folloews. 1. Source Source of mudanpi is cortex of Paeonia suffruticosa Andrews(Family:Ranunculaceae) in korea and china. It needs an attention to distinguish into because there is a substitute and a rank in china. 2. process We have to work before harvesting fresh roots is dry perfectly. If outer skin dried moisture a little, cut off outer skin vertically at sunny day, remove a heart and sever, immedietly dry. When working, we have to take care of because of easy discoloration with moisture. 3. Quality (1) Functional standards A diameter is large, it do not exist a heart of wood, a bark is thick and powdery. Section surface is light white color and rich odor with many light crystals. (2) Physicochemical stamdards We think that it needs to raise a contant of paeonol at all kinds of testing standards. A contant of paeonol is at least more than 2.5%. A part of wood indicate a heart of wood which has no medical effects, but is a relatively heavy part. So a standart of wood must be lowered sifnificantly less than 1.0%. It must be content of ash is less than 6.0%, Content of acid-nonsoluble ash is less than 1.0%. A fixed quantity of paeonol is more than 2.5%. Contens of heavy metal has to detect less than 30 ppm and there is no reminding agriculural medince.

• Human Ecology Research
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• 제54권3호
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• pp.251-262
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• 2016

This study analyzes the relation of the consumption values and ethical fashion consumption behavior as well as other influential factors between Korean and German ethical consumers based on the 'Modified Theory of Planned Behavior.' A survey was conducted on female consumers in their 20s and 30s, who experienced ethical fashion consumption in Seoul and Berlin. A factor analysis, reliability analysis, F -test and multiple regression analysis were performed for model verification. The research results indicated that emotional value (in case of Korea) and functional value out of consumption values (in case of Germany) have significant effects in regards to the influence of consumption values on ethical self-identity. The effects of ethical self-identity on ethical fashion consumption behavior indicated that Korean ethical self-identity influenced the ethical fashion consumption behavior of moderating and simplicity and eco-friendly certification versus local consumption as well as above factors (in case of Germany) explained by ethical self-identity. Both variables had a negative moderating effect in Korea in regards to the moderating effects of social comparison and materialism in the relations of ethical self-identity and ethical fashion consumption behavior; however, only materialism was an influential factor in Germany. The results of the research variables by individualism/collectivism indicated that the horizontal-collectivism group showed the highest ethical self-identity along with the lowest materialism tendency that had a high point of consumption behavior towards local consumption. However, the vertical-individualism group was outstanding. The consumption behavior aimed at moderating and simplicity.

• 재활간호학회지
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• 제4권1호
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• pp.118-123
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• 2001

The purpose of this study was to provide fundamental data to propose further directions of education on rehabilitation nursing by investigating the adequacy of the educational contents of rehabilitation nursing. This study was a descriptive survey study. The data collected at 25 universities and 24 junior colleges through questionnaires to answer the research questions from August 10 to September 30, 2000. The questionnaire was consisted of 24 items. The contents of rehabilitation nursing education were developed by consulting with the rehabilitation nursing professionals. The results are as follows Rehabilitation nursing was taught as an independent class in 15 universities and 9 junior colleges. Most professors majoring in adult nursing (66.8%) were in charge of teaching the courses. For the adequacy of the teaching contents of rehabilitation nursing, conceptual bases for rehabilitation was the highest score (4.0), and interdisciplinary rehabilitation team, activities of daily living, clients of rehabilitation, nursing process in rehabilitation nursing, functional evaluation, movement and mobility, physical therapy, occupational therapy, sensation and perception, communication/language, eating and swallowing, bladder elimination, community based rehabilitation nursing, sleep, rest &, fatigue, bowel elimination, historical perspectives of rehabilitation nursing, sexuality, pulmonary rehabilitation, pain, cardiac rehabilitation, skin integrity, family care was ordered.

• 한국농촌건축학회논문집
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• 제18권2호
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• pp.29-37
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• 2016

This study is aimed to suggesting a hypothesis about the process of improvement of houses of the Korean-Chinese, comparing how Han Chinese and Korean-Chinese houses changed in Yanbian Area of China. This draws the conclusion by analyzing the field survey about residence of the Han Chinese and the Korean-Chinese in Yanbian Area of China. First of all, the characteristic of residence style change and the plan of rural houses of the Han Chinese are in the following. The plan of houses has been changed from allocating rooms from side to side with the kitchen as the center to linking each functional spaces through the aisle. And the main bedroom of houses becomes the central space for family members instead of Kang (partial Ondol) by expanding the floor heating system under the whole floor in houses. Next, the feature of residence style changes of the Han Chinese and the Korean-Chinese is as follows. In 1970s barns disappeared from houses of the Korean Chinese, but houses of the Han Chinese were not much changed. From 1980s to 1990s, spaces linking each rooms with aisles had appeared for comfortableness of residence and respecting each individual's privacy. On the other hand, The houses of the Korean-Chinese make a whole big space by getting rid of walls between the rooms. And after 2 thousand years later, Han Chinese houses have a bedroom with floor heating system. Both houses of the Korean-Chinese and the Han Chinese are changed to have a residence style which taking sedentary style and standing style.