• 한국잠사학회:학술대회논문집
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• 한국잠사학회 2003년도 International Symposium of Silkworm/Insect Biotechnology and Annual Meeting of Korea Society of Sericultural Science
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• pp.53-54
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• 2003

The silk fibroin has been widely used as industrial materials such as cosmetics and food l)-2). In this study, general hydrolysate of B. mori fibroin as a biomaterial, development of functional-cometics included hydrolysate of B. mori fibroin will be discussed. (omitted)

• 생명과학회지
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• 제19권1호
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• pp.147-151
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• 2009

D-pinitol, another chemical structure of 3-O-methyl-D-chiro-inositol, is an important insulin-sensitizer. The purpose of this review is to examine the characteristics of pinitol and other analogs as functional food biomaterials which were well known to reduce blood glucose levels. Pinitol can be converted to chiro-inositol in normal humans, while diabetic patients can not use the molecule, resulting in exhibiting low level of chiro-inositol in their urine. Recently, it is reported that pinitol can trigger phospholipase C/D, thus the rate of glucose metabolism accelerates to use as fuel for human body. To not only reduce insulin resistance of diabetic patients, but also alleviate the symptoms of diabetes, obesity, and muscle contraction, pinitol and its dietary supplementation is needed.

• Journal of Microbiology and Biotechnology
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• 제19권1호
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• pp.51-54
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• 2009

Myxobacteria, Gram-negative soil bacteria, are a well-known producer of bioactive secondary metabolites. Therefore, this study presents a methodological approach for the high-throughput screening of secondary metabolites from 4 wild-type Myxococcus xanthus strains. First, electrospray ionization mass spectrometry (ESI-MS) was performed using extracellular crude extracts. As a result, 22 metabolite peaks were detected, and the metabolite profiling was then conducted using the m/z value, retention time, and MS/MS fragmentation pattern analyses. Among the peaks, one unknown compound peak was identified as analogous to the myxalamid A, B, and C series. An analysis of the tandem mass spectrometric fragmentation patterns and HR-MS identified myxalamid K as a new compound derived from M. xanthus. In conclusion, LC-MS/MS-based chemical screening of diverse secondary metabolites would appear to be an effective approach for discovering unknown microbial secondary metabolites.

• Journal of Applied Biological Chemistry
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• 제52권2호
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• pp.45-50
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• 2009

본 연구에서는 개비자나무(Cephalotaxus koreana)를 기능성 생물소재로 개발하기 위해 개비자나무 추출물(Korean Plum-yem extract, KPE)의 항균활성, 암세포 증식 억제활성 및 독성을 확인하였고, 일반성분을 조사하였다. KPE의 농도에 따른 항균활성은 그램 양성균, 그램 음성균, 효모 및 사상균에 대하여 성장 저해현상을 나타내었으며, 특히 그램 음성균인 Pseudomonas aeruginosae가 KPE에 가장 민감하게 생육 저해현상을 보였고, 마찬가지로 그램 음성균인 Salmonella thyphimurim, Escherichia coli 순이었다. 그램 양성균이 그램 음성균에 비해 KPE에 의한 생육저해효과가 작은 것으로 나타났다. 혈액암 세포주를 이용한 KPE의 암세포 증식 억제활성을 분석한 결과 80 ${\mu}g/mL$의 농도에서 현저한 암세포 저해활성을 보였다. 또한, KPE를 섭취하였을 때 유발되는 용혈율을 측정하여 KPE의 적혈구 막지질 붕괴의 유무를 조사한 결과, KPE 중 hemolysis를 일으키는 물질은 KPE의 유효성분인 HHT 외에 다른 물질이 있는 것으로 판단되었으며, Ame's test에 의한 돌연변이원성을 갖지 않음을 확인하였다. 따라서 개비자나무 추출물은 항균과 암세포에 대한 세포독성 등 기능성을 갖는 천연물 소재로서 이를 응용한 기능성 식품 개발이 가능한 것으로 판단된다.

• KSBB Journal
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• 제29권6호
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• pp.437-442
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• 2014

In vitro biological activities of Aloe vera gel and skin extracts were evaluated. Total polyphenol contents of Aloe vera skin were measured 41.12 mg/g. DPPH radical scavenging activity of Aloe vera skin-70% EtOH extract, Aloe vera skin-water extract, Aloe vera gel-70% EtOH extract and Aloe vera gel-water extract were 55%, 38%, 11% and 10%, respectively. In addition, 70% EtOH extract and water extract were compared with respect to SOD-like antioxidant activity of Aloe vera-70% EtOH extract has higher activity than Aloe vera water extract. Tyrosinase inhibition rate of Aloe vera gel extract was higher than Aloe vera skin extract. Alcohol dehydrogenase (ADH) and Acetaldehyde dehydrogenase (ALDH) relative percentage activity of Aloe vera gel extract were 126% and 216%, respectively. It was suggested that Aloe vera gel and skin extracts could be used as a functional biomaterial for functional food and cosmetics.

• 접착 및 계면
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• 제8권3호
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• pp.16-23
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• 2007

폴리에스테르 편 직물들을 전류세기 5, 10, 15, 20 A로, 공급속도 5, 10, 15 m/min로 코로나 처리하였다. 이들의 표면변화를 주사전자현미경(SEM)과 X-ray 광전자분석기(XPS)로 확인하였다. 또한 물리적 성질의 변화를 인장강도, 건조 시와 습윤 시의 접착강도를 통하여 측정하였다. 접착에 사용된 접착제는 열경화성 반응형 폴리우레탄 핫 멜트 접착제이다. 대기압에서 코로나 방전처리에 의해 폴리에스테르 편 직물에 관능기들이 도입되어졌고, 표면에 요철이 발생하여 그 결과 접착력은 향상되었다. 건조 시 및 습윤 시의 접착강도를 모두 고려할 때, PET 직물의 코로나 처리에 있어 적절한 조건은 공급 속도 10 m/min, 전류 세기 15 A라 생각되며, mesh형 PET knit는 공급 속도 10 m/min, 10 A 이상의 전류세기라 생각된다.

• 동의생리병리학회지
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• 제20권1호
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• pp.65-68
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• 2006

It was examined the effect of fermented rice wine using mycelium of Phellinus linteus (FWPL) on the gastric mucosa of rat. The gastric mucosal lesions were not seen macroscopically in normal, but ethanol-administrated rats produced congestion and edema with a few local lesions. The administration of FWPL showed a similar pattern as like normal except trace histopathological changes. The results of Western blot analyses showed that the higher expression of inducible nitric oxygenase (iNOS), cyclooxygenase (COX)-1, COX-2, tumor necrosis factor-$\alpha$ and c-fos, especially COX-2, in the ethanol-administrated rat compared with normal rat. But FWPL-administrated rat showed a trace increase of these expression compared to normal rat. About immunohistochemical observations, weaker iNOS reactions were detected in mucous cells of epithelim of ethanol administrated rat compared with normal and FWPL-administrated rat. These results suggested that FWPL-administrated rat showed a trace changes on the mucus barrier-related protein expression compared with ethanol-administrated rat and thus FWPL may be use to develop a functional alcoholic beverage.

• Journal of Applied Biological Chemistry
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• 제59권3호
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• pp.239-242
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• 2016

A novel Nicotinoyl fused peptide, Nicotinoyl-LVH, was synthesized by solid phase peptide synthesis method, purified, and tested in cultured skin cells. Nicotinoyl-LVH enhanced the expression level of collagen mRNA and its fragments in fibroblasts. These data show that this novel Nicotinoyl peptide is a promising biomaterial in the anti-aging functional cosmetic market.

• 한국생물공학회:학술대회논문집
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• 한국생물공학회 2005년도 생물공학의 동향(XVI)
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• pp.296-299
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• 2005

Sugar polymers have been considered as biomaterial. Biomaterials are widely utilized for a medical applications in direct contact with living tissue Clearly, biomaterials must be carefully and microscopically fabricated for optimal acceptance within the living organism in both functional and structural senses. In this study, the enzymatic synthesis of sorbitan methacrylate from 1,4-sorbitan via the manipulation of an immobilized biocatalyst (Novozym 435) and acryl donors (methacrylic acid and vinyl methacrylate) was evaluated.

• 한국재료학회:학술대회논문집
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• 한국재료학회 2011년도 추계학술발표대회
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• pp.2.2-2.2
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• 2011

The central strategy in tissue engineering involves a biomaterial scaffold as a delivery carrier of cells and a depot to deliver bioactive molecules. The ability of scaffolds to control cellular response to direct particular repair and regeneration processes is essential to obtain functional tissue engineering constructs. Therefore, many efforts have been made to understand local interactions of cells with their extracellular matrix (ECM) microenvironment and exploit these interactions for designing an ideal scaffold mimicking the chemical, physiological, and structural features of native ECM. ECM is composed of a number of biomacromolecules including proteins, glycosaminoglycans, and proteoglycans, which are assembled together to form complex 3-dimensional network. Electrospinning is a process to generate highly porous 3-dimensional fibrous structure with nano to micro scaled-diameter, which can closely mimic the structure of ECM. In this presentation, our approaches to develop biomimetic electrospun fibers for modulation of cell function will be discussed.