• Korean Journal of Breeding Science
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• v.42 no.4
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• pp.374-380
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• 2010

In order to select excellent cross parents in strawberry breeding, 11 varieties were self-pollinated and tested their progenies. Among the 11 varieties, 'Akihime', 'Maehyang' and 'Seolhyang' showed superior in plant vigor, disease resistance and yield. Combining ability was tested using 15 progenies obtained from crosses between 3 selected varieties as seed parents and 5 varieties as pollen parents. Plant vigor was the strongest when 'Akihime' was used as a seed parent while ripening time tended to the fastest when 'Seolhyang' as a seed parent. Average fruit weight of the crosses 'Seolhyang' ${\times}$ 'Tochiotome', 'Seolhyang' ${\times}$ 'Geumhyang', 'Seolhyang' ${\times}$ 'Wongyo 3111' and 'Akihime' ${\times}$ 'Wongyo 3111' were above 18 g for marketable fruits. Fruit hardness was superior at crosses of 'Maehyang' as a seed parents to other crosses combinations, especially 'Maehyang' ${\times}$ 'Tochiotome'. Soluble solid contents (SSC) was not significantly different among cross combinations. The best cross combination for marketable yield was 'Akihime' ${\times}$ 'Wongyo 3111', followed by 'Seolhyang' ${\times}$ 'Geumhyang', 'Maehyang' ${\times}$ 'Geumhyang', 'Akihime' ${\times}$ 'Tochiotome', in order.

• Horticultural Science & Technology
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• v.32 no.6
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• pp.917-921
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• 2014

'Hei-G' is a new tomato cultivar originating from the cross of two inbred lines, the maternal plant TKUI-3 separated from 'Kame' in 2007, and the paternal plant AL-1 developed by pedigree breeding. The new cultivar was registered as 'Hei-G' named 'JTB026 (Jeonnam-2)' based on characteristics and yield trials carried out in the spring of 3 years, from 2010 to 2012. 'Hei-G' is an indeterminate type with strong plant vigor. The fruit is round and the ripening fruit is blackish red. The average fruit weight of 'Hei-G' is about 43.4 g, and its marketable yield is $4,944kg{\cdot}10a^{-1}$. The lycopene content of 'Hei-G' ($11.8mg{\cdot}100g^{-1}$) is higher than that of other tomatoes (e.g., cv. Rapido, 3.1 mg). In addition, 'Hei-G' is suitable for environment-friendly protected cultivation because of its resistance to powdery mildew.

• Journal of Life Science
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• v.19 no.1
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• pp.152-155
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• 2009

The old-gold-crimson ($og^c$) fruit color mutation produces deep red tomato fruit with high lycopene content. age is a null mutation allele of lycopene-${\beta}$-cyclase (Crt-b) gene (B locus) that converts lycopene to ${\beta}$-carotene in the cartenoid biosynthesis pathway in tomato. Breeding of high lycopene tomato cultivars can be accelerated by marker-assisted selection (MAS) for introgression of $og^c$ allele by using a gene-based DNA marker. In order to develop a marker, single nucleotide deletion of adenine(A) with. in a poly-A repeat that has been known to be responsible for frame-shift mutation of $og^c$ was confirmed by resequencing mutant allele and wild-type allele at B locus of several tomato lines. For allele discrimination and detection of $og^c$, derived CAPS (dCAPS) approach was used by designing a primer that artificially introduced restriction enzyme recognition site of Hin fI in PCR products from $og^c$ allele. This dCAPS marker is co-dominant gene-based PCR marker that can be efficiently used for MAS breeding program aiming the development of high lycopene tomato.

• Journal of Sericultural and Entomological Science
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• v.52 no.2
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• pp.147-154
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• 2014

We bred a mulberry cultivar named Palcheong, through local adaptability test, which is under registration as a new cultivar for fruit production. Local adaptability test had been carried out at four places (Suwon, Kongju, Wanju, and Jinju) for seven years from 2007. This is belonging to (Morus Lhou(Ser.) Koidz.) selected among mulberry genetic resources preserved in Suwon. Its sex expression was hermaphrodite, which can be used for the production of seedling for fruit production by artificial crossing as pollen parent Palchung showed lower yielding in fruit productivity by 16% compared to control cultivar 'Chungil (Morus alba L.)' for five years. Although Palchung was higher in sugar content of mulberry fruits, it was bigger than that of 'Chungil'. It is adaptable to every where except the places where cold damage and sclerotic disease happen frequently.

• Journal of Plant Biotechnology
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• v.43 no.4
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• pp.466-472
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• 2016

This study analyzed the genetic diversity of 115 pear germplasms using 15 SSR markers. Three to forty-one SSR alleles were detected for each locus with an average of 16 alleles per locus. The average availability of markers was 0.966. The average observed heterozygosity ($H_{obs}$) was 0.603 (range: 0.140 to 0.929). The average expected heterozygosity ($H_{exp}$) was 0.718 (range: 0.463 to 0.904). The average polymorphism information content (PIC) was 0.692 (range: 0.403 to 0.897). The genetic relationships of pear germplasms were classified into two major groups by geographic origins and genetic characteristics according to genetic distance. The first group was composed of European pear belonging to Pyrus communis. The second group consisted of P. pyrifolia, P. ussuriensis, P. bretschneideri, P. betulaefolia, P. calleryana, interspecific hybrids, and unclear germplasms. The results of this study suggest that genotype analysis of pear germplasms using SSR markers can identify the genetic diversity of germplasms, and can be used to provide basic information for pear breeding.

• Horticultural Science & Technology
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• v.29 no.6
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• pp.655-658
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• 2011

A new cultivar 'Hongan' was originated from an artificial cross between 'Fuji' and 'Jonathan' carried out at National Institute of Horticultural & Herbal Science in 1993. The cultivar was preliminarily selected among the elite siblings for its high fruit quality in 2002. After regional adaptability test at five districts for subsequent four years as 'Wonkyo Ga-32', it was finally selected in 2006. 'Hongan' is characterized by the following. Optimum harvest time is late September. Shape of the mature fruit at 309 g on mean weight is globosity with a light red skin on a greenish yellow ground and yellowish white flesh. The fruit contains favorable total soluble solids at $13.7^{\circ}Brix$ and an acidity at 0.28%. Safe storage period for the fruit is 3 weeks at room temperature. It is resistant to Bitter rot. 'Hongan' also reveals a physiological cross compatibility with leading cultivars such as 'Gamhong' and 'Tsugaru' in addition to its maternal parent 'Fuji'. Tree topology is semi-spreading with vigorous growth habit.

• Horticultural Science & Technology
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• v.29 no.5
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• pp.507-509
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• 2011

'Heukisul' (3x) a cross made in 2000 between 'Kyoho' (4x, Vitis sp.) and 'Thompson Seedless' (2x, Vitis vinifera L.) in a seedless grape breeding program, was preliminarily selected in 2004. After regional adaptation testing under the name of 'KTS014' at two sites during 2004-2005, it was finally selected in 2006. 'Heukisul' showed low incidence of berry shattering, resulting in a good berry set compared to 'King Dela'. 'Heukisul' had budburst on April 23, flowering on in June 9, and fruit maturation in September 29 (almost the same as 'King Dela' at Chuncheon), and it was considered a midseason cultivar. The mean berry weight was 4.0 g, about 0.7 g heavier than 'King Dela', and mean soluble solids were $21.9\;^{\circ}Brix$, about $2.7\;^{\circ}Brix$ higher than 'King Dela'. The skin color was dark violet with abundant bloom and the flesh was very firm. Although the cluster was compact, it required no cluster thinning. Also the incidence of berry cracking was very low.

• BMB Reports
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• v.38 no.3
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• pp.320-327
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• 2005

Five ripening-related ACC synthase cDNA isoforms were cloned from 80% ripe papaya cv. 'Sinta' by reverse transcription-PCR using gene-specific primers. Clone 2 had the longest transcript and contained all common exons and three alternative exons. Clones 3 and 4 contained common exons and one alternative exon each, while clone 1, the most common transcript, contained only the common exons. Clone 5 could be due to cloning artifacts and might not be a unique cDNA fragment. Thus, there are only four isoforms of ACC synthase mRNA. Southern blot analysis indicates that all five clones came from only one gene existing as a single copy in the 'Sinta' papaya genome. Multiple sequence alignment indicates that the four isoforms arise from a single gene, possibly through alternative splicing mechanisms. All the putative alternative exons were present at the 5'-end of the gene comprising the N-terminal region of the protein. 'Sinta' ACC synthase cDNAs were of the capacs 1 type and are most closely related to a 1.4 kb capacs 1-type DNA(AJ277160) from Eksotika papaya. No capacs 2-type cDNAs were cloned from 'Sinta' by RT-PCR. This is the first report of possible alternative splicing mechanism in ripening-related ACC synthase genes in hybrid papaya, possibly to modulate or fine-tune gene expression relevant to fruit ripening.

• Horticultural Science & Technology
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• v.17 no.1
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• pp.15-16
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• 1999

'Cheonhong'was released by the National Horticultural Research Institute in 1992 as a new mid-ripening, yellow-fleshed, and freestone nectarine variety, which originated from open-pollinated seeds of 'Garden State' nectarine collected in 1978. This was tested as 'Wonkyo Da-03' at 4 areas for 4 years from 1989. 'Cheonhong' is resistant to fruit cracking during rainy season and russeting. Fruits mature about 15 days before 'SunGlo' and have rich-flavor and good quality.

• Horticultural Science & Technology
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• v.34 no.2
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• pp.257-268
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• 2016

Physiological characteristics of five new apple cultivars from the Korean apple breeding program were evaluated as a function of harvest time and storage after harvest. Internal ethylene concentration (IEC), flesh firmness, soluble solids concentration (SSC), and titratable acidity (TA) were measured in 'Summer Dream', 'Summer King', 'Green Ball', 'Picnic', and 'Hwangok' apples at harvest, during shelf life at $20^{\circ}C$, and one day after cold storage at $0.5^{\circ}C$ in air. IEC increased during shelf life in 'Summer Dream', 'Summer King', and 'Green Ball' but not in 'Picnic' or 'Hwangok', regardless of harvest time. Flesh firmness decreased towards harvest time and decreased gradually with time in cold storage only in the former three cultivars. In turn, IEC increased during cold storage in the first three cultivars but not for the last two cultivars, irrespective of harvest time. Changes in SSC and TA did not consistently relate to harvest time or storage period but TA tended to decrease as IEC increased. Furthermore, IEC was negatively correlated with flesh firmness except in the 'Green Ball' cultivar but the significance level was much greater in 'Summer Dream' and 'Summer King' (p < 0.0001) than in 'Picnic' (p < 0.01) or 'Hwangok' (p < 0.05) cultivars. Flesh firmness was positively correlated with TA in the first three cultivars but not in the last two cultivars. Overall, the results indicate that cultivars for which IEC increased after harvest had reduced flesh firmness and TA after storage.