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http://dx.doi.org/10.5010/JPB.2016.43.4.466

Genetic relationships of pear germplasms using simple sequence repeat marker  

Chun, Jae An (Fruit Research Division, National Institute of Horticultural & Herbal Science, Rural Development Administration)
Cho, Kang Hee (Fruit Research Division, National Institute of Horticultural & Herbal Science, Rural Development Administration)
Kim, Se Hee (Fruit Research Division, National Institute of Horticultural & Herbal Science, Rural Development Administration)
Lee, Han-Chan (Fruit Research Division, National Institute of Horticultural & Herbal Science, Rural Development Administration)
Choi, In Myong (Fruit Research Division, National Institute of Horticultural & Herbal Science, Rural Development Administration)
Park, Seo Jun (Fruit Research Division, National Institute of Horticultural & Herbal Science, Rural Development Administration)
Publication Information
Journal of Plant Biotechnology / v.43, no.4, 2016 , pp. 466-472 More about this Journal
Abstract
This study analyzed the genetic diversity of 115 pear germplasms using 15 SSR markers. Three to forty-one SSR alleles were detected for each locus with an average of 16 alleles per locus. The average availability of markers was 0.966. The average observed heterozygosity ($H_{obs}$) was 0.603 (range: 0.140 to 0.929). The average expected heterozygosity ($H_{exp}$) was 0.718 (range: 0.463 to 0.904). The average polymorphism information content (PIC) was 0.692 (range: 0.403 to 0.897). The genetic relationships of pear germplasms were classified into two major groups by geographic origins and genetic characteristics according to genetic distance. The first group was composed of European pear belonging to Pyrus communis. The second group consisted of P. pyrifolia, P. ussuriensis, P. bretschneideri, P. betulaefolia, P. calleryana, interspecific hybrids, and unclear germplasms. The results of this study suggest that genotype analysis of pear germplasms using SSR markers can identify the genetic diversity of germplasms, and can be used to provide basic information for pear breeding.
Keywords
Pyrus; SSR marker; Genetic diversity; Germplasms;
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