• 대한의생명과학회지
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• 제13권4호
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• pp.361-368
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• 2007

Biological samples can be fixed either by chemical method by using chemical solution or physical methods by using heat treatment. The problem in traditional heat fixation is unsatisfactory quality due to uneven heat conduction in specimen and loss of inner cell contents. Chemical fixation method also bears several intrinsic problems like the limit in specimen size, time consumption in fixative impregnation, and loss of low molecular weight cell components. These factors deteriorate the quality of fixed specimen, thus limit the magnification and contrast of tissue pictures. Microwave heat has been reported to be a good alternative to current chemical methods to overcome these problem. In this study, we tried to introduce the microwave energy method to routine fixation work in hospital. We replaced chemical fixative with saline to provide moderate reaction condition, and used frozen section to reduce time for sample preparation. Temperature was measured at each experiment. The fixation of rat kidney tissue with 2.45 GHz electromagnetic wave and saline showed similar result to the control group fixed with traditional chemical method. Human tumor tissue fixed with 2.45 GHz electromagnetic in frozen section was improved in terms of histochemistry of PAS and immunohistochemistry of tumor marker like cytokeratin. Total turnaround time was reduced from $24\sim38$ h to to $2\sim4$ h. In conclusion, the quality of samples prepared by microwave heating method was at least as good as that of traditional method. If the condition for the fixation of different specimens is standardized, this new method could be applied to routine work in hospital, and could save working time as well.

• 한국수산과학회지
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• 제37권3호
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• pp.175-178
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• 2004

We here report that the muscle of 'gukmeri-bok' puffer fish (Takifugu vermicularis radiatus) is intoxicated after freezing and thawing processes even though it is not toxic when it is fresh. This study was carried out to investigate the effect of different freezing and thawing conditions as well as the effect of the presence of skin or viscera on the intoxication of muscle of 'gukmeri-bok' puffer during freezing and thawing process. As a result of this investigation, thawing condition, but not freezing conditions or periods played an important role in muscle intoxication. Tetrodotoxin secretory grands exist in the skin of toxic puffer fish, the skin was more responsible for the muscles intoxication than the viscera during the freezing-thawing process. In other words, no toxicity was measured in the muscle of skinned-frozen specimens even when thawed. According to this result, it is recommended that the skin and viscera must be removed before being frozen for edible purpose. Otherwise, when a whole fish should be frozen, alternative half-thawing and removal of skin from frozen specimens is recommended.

• Journal of Korean Society for Atmospheric Environment
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• 제22권E1호
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• pp.27-34
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• 2006

Although the importance of size-resolved raindrops study has been known, it has not been popularized up to the present. In the present study, an attempt was made to generalize the size-resolved raindrops study by a combination of the frozen raindrop collection method and a commercially available high-performance capillary electrophoresis (HPCE). Samplings were carried out at Kyoto, Japan in October 2002. The inorganic ions (chloride, nitrate, sulphate, calcium, ammonium, sodium, magnesium, potassium) in size classified raindrop samples were successfully analyzed by HPCE with good repeatability. To assure the accuracy and precision of HPCE data, t-test was conducted with paired analytical data, which were experimentally constructed by analyzing standard solutions with HPCE and IC, respectively. T-test showed that there is no notable difference between the concentrations determined by the two analytical methods. Every ionic concentration in both cation and anion was found to be strong raindrop size dependence. Though there was slight increase of sodium and sulphate concentrations between 0.85 mm and 1.15 mm raindrop radius, it showed a strong decrease for every ionic component with increasing droplet radius. The combination of the frozen raindrop collection method and a commercially available HPCE can meet the need of size-resolved raindrops study.

• 동아시아식생활학회지
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• 제24권6호
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• pp.785-792
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• 2014

This study investigated the optimal mix ratio of frozen-dried loquat fruit powder and the optimun conditions for making Seolgidduk by analyzing chemical properties, rating moisture contents, color, mechanical quality characteristics and sensory characteristics Seolgidduk was made with 0, 2, 4, 6 and 8% frozen-dried loquat fruit powder, which contains functional components. Chemical analysis showed that loquat fruit powder contained of 12.87% water, 2.23% crude protein, 0.55% crude fat, 3.57% crude ash and 3.89% crude fiber. Water content of Seolgidduk containing loquat fruit ranged from 36.58~39.18%. With more loquat fruit powder L value decreased, while the 'a' value and 'b' values increased. According to scanning electronic microscopy, Seolgidduk containing frozen-dried loquat fruit powder showed weak coherence. Hardness decreased with more frozen-dried loquat fruit powder. In the sensory test, Seolgidduk with 4% loquat fruit powder was the most preferred with less sourness, proper softness, moisture and chewiness. Based on the results of this experiment, Seolgidduk with 4% loquat fruit powder showed less hardness than the control group. These results show that quality and preference increased when 4% loquat fruit powder was added to Seolgidduk. Therefore, Seolgidduk with 4% of loquat fruit powder is expected to increase quality and preference of Seolgidduk.

• 한방재활의학과학회지
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• 제18권3호
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• pp.81-90
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• 2008

Objectives : The purpose of this study was to identify the effect of electro-acupuncture stimulation regarding restoration of range of motion on patients with frozen shoulder. Methods : Experimental group measured with shoulder flexion and abduction angle at pre and post electro-acupuncture stimulation. Results : There were statistically significant difference regarding change of flexion angle of electro-acupuncture stimulation(p<0.01) and the testing value of all levels had a significant difference with the value of final post-test(p<0.01). There were statistically significant difference regarding change of abduction angle of electro-acupuncture stimulation(p<0.01). Conclusions : Electro-acupuncture stimulation of distal part increased range of motion of frozen shoulder. Therefore, application of electro-acupuncture should be developed as a therapeutic method in treatment of frozen shoulder. Also, it can contribute as one of good therapeutic methods offered to patients through approach of oriental rehabilitation medicine.

• Clinical and Experimental Reproductive Medicine
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• 제17권2호
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• pp.129-135
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• 1990

This study was done to verify factors affecting viability after cryopreservation and pregnancy rate after frozen-thawed embryo transfer into uterus. Embryos were cryopreserved slow freezing and slow thawing and used DMSO as cryoprotectant. The results were to follows. 1. Viability of frozen-thawed embryos were 75.5% (94/105), which compared with viability of embryos according to cell stage, $2{\sim}5$ cell was 68.4% and $6{\sim}16$ cell 80.4% were significant differences (p<0.05). 2. No significant difference in duration of cryopreservation on effects affecting pregnancy rate was observed. 3. Number of embryo transfered into uterus was significant differences (p<0.05). 4. Four pregnancies resulted following replacement of 35 frozen-thawed.

• 한국지반공학회논문집
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• 제36권3호
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• pp.25-36
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• 2020

동토의 세립분 함유량에 따른 파괴특성을 파악하기 위해 -10℃의 온도에서 다양한 세립분 함유량과 초기 노치(notch)의 위치를 조정한 직사각형 공시체를 제작하여 Three-point bending 시험을 수행하였다. 시험결과를 바탕으로 동토의 mode I 파괴인성(fracture toughness)을 산정하였으며, 하중-변형 곡선의 최대점까지의 fracture energy를 산정하여 동토의 mixed-mode(mode I + II) 파괴특성을 파악하였다. 시험결과, 최대하중 및 mode I 파괴인성은 세립분 함유량 10%까지 증가하다가 15%에서 다시 감소하는 경향을 나타내었다. 또한, 노치의 위치가 공시체 중심에서 멀어질수록 mode II 하중의 증가로 인해 균열이 진행하는데 필요한 fracture enenrgy가 증가하는 것으로 나타났으며, 세립분 함유량이 증가할수록 mode II 하중의 증가비율 또한 증가하는 것으로 나타났다.

• 한국수정란이식학회지
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• 제14권3호
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• pp.163-169
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• 1999

This study was carried out to investigate the effect of co-culture system(bovine oviduct epithelial cells; BOEC) and defined culture system(modified TALP ; mTALP) on the development of IVM-IVF embryos, and survival of in vitro produced blastocysts after freezing and thawing. Occytes from the slaugheterhous ovaries were matured and fertilized using general protocol. The results obtained were as the following: 1. Survival rates of frozen-thawed blastocysts using 10% glycerol as cryoprotectant was higher in day 7 blastocysts than in Day 8 and 9 blastocysts from co-cultrue system, but survival rate of frozen-thawed blastocysts was higher in Day 10 blastocysts than in day 8 and 9 blastocysts from defined culture system. Regardless of their age, survival rate of frozen-thawed blastocysts was significantly higher (p<0.05) in co-culture system than in defined culture system. 2. The cell number of blastocysts was significanlty higher (p<0.05) in Day 7 blasotcysts than in Day 8 and 9 blastocysts from co-cultures, but the cell number of blsstocysts was significantly higher (p<0.05) in Day 10 blastocysts than in Day 8 and 9 blastocysts from defined culture system. Regardless of the culture system, blastocysts with higher cell number showed higher survival rates after freezing and thawing.

• 한국식품조리과학회지
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• 제17권2호
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• pp.149-155
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• 2001

Namul cooked with the standard recipe was examined by research of microbiological test for three months and sensory evaluation of frozen namul after reheating. When the namul was freezed storage, in the microbiological test namul began to change on the 40th day, but there was no problem about stability of storage until 3 months. The overall qualities of taste, flavor, color and texture were examined by sensory evaluation of frozen namul after reheating. The pH was seemed to change slowly, its color was changed on the 20th day from the beginning of storage. Radish root represented substantial difference in texture and overall quality on the 20th day. The off-flavor of immature pumpkin stated on the 25th day. Later 10days nettle tree mushroom began to be changed in its taste, texture, overall quality and appearance, then on the 25th day it was seemed to have low preference. But oak mushroom kept its quality good for 25 days. Oyster mushroom was changed in color, appearance and overall quality on the 20th day. Bracken had low preference in taste, texture and moisture on the 25th day. The color of spinach was changed on the 15th day, and its taste on the 20th day. Soybean sprout was changed in taste, texture and overall quality on the 15th day, and overall quality marked low preference on the 25th day Root of bell flower was changed on the 25th day(p<0.05).

• Preventive Nutrition and Food Science
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• 제3권2호
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• pp.157-162
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• 1998

As a part of investigation for listeriosis, we attmepted iolationof Listeria spp. from commerical frozen and refrigerated foods at the supermarket level. Seven strains of Listeria spp. were isolate from 6 samples (7.15) among 84 samples of frozen foods, and ten strains of Listeria spp. were also isolated from 8 samples (7.6%)_ among 105 samples of refrigerated foods at the supermarkets in Pusan area. From a total of 189 commerical foods, the number of isolated Listeria spp. and ratio were 6 strains(3.25) of L. grayi, one strain (0.5%) of L. welshimeri, 6 strains (3.2%) of L. innocua and 4 strains (2.15) of L monocytogenes. Listeria spp. isolates except L. monocytogenes did not show $\beta$-hemolysis on blood agar and positive reaction in CAMP test with Staphyloccccus aureus. In the antibiotic susceptibility,most isolates of Listeria spp. were susceptible to 12 antibiotics such as ampicillin , cephalotin, penicillin, amikacin, gentamicin, erythromycin, kanamycin, vancomycin, tobramycin, carbenicillin , tetracycline and trimethoprim /sulfamethoxiazole. Four strains of L. monocytogenes were susceptible to ntibiotics used in this study except nitrofurantio. The serotype of 3 strains and one strain of L. monocytogenes were classified into Listeria O serotype 1 and 4, respectively.