• Parasites, Hosts and Diseases
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• v.22 no.1
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• pp.127-134
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• 1984

As a series of studies to clarify clonorchicidal substances in body surface mucus of some freshwater fishes, the substance in the epidermal mucus of Cyprinus carpio was isolated by silica gel column and thin layer chromatography and analysed for its chemical nature. Wormicidal trial was done in vitro, and the results obtained are summarized as follows: The mucus was extracted by ethyl ether and separated into 4 fractions by column chromatography using benzene as solvent. The second fraction with yellowish red colour among them showed the strongest clonorchicidal effect. The yellowish red fraction obtained by column chromatography was then fractionated into 6 spots by thin layer chromatography with petrol/ etherjchloroform (30/70, v/v), and the Rf. 0.714 spot among the 6 spots showed the strongest effect. The Rf. 0.714 spot was further fractionated into 6 spots by thin layer chromatography with benzene/acetone (90/10, vlv), and the Rf. 0.800 spot among the later 6 spots revealed the strongest effect. The Rf. 0.800 spot was chromatographed on column with benzene and 2 fractions were obtained. The second fraction of light brown colour represented the final purified fraction. By these Purification Procedures, clonorchicidal substance was Purified 15-fold with 0.035 yield from the mucus of C. carpio, and 10mg of the final fraction killed the cercaria in 26 min, the metacercaria in 115 min, and the adult in 443 min. Infra red and nuclear magnetic resonance spectrometric analysis of the purified substance revealed that the substance belongs to an ethyl ester of unsaturated fatty acid with 2 double bonds, 15 methylene groups and 1 methyl group.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.39 no.6
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• pp.519-525
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• 1994

This experiment was conducted to investigate the effects of sowing date and cutting time on feed value of rye in paddy field at Yesan from Oct. 1990 to June 1991. The field experiment was sown 5 times at 10 day intervals from Oct. 5. The content of crude protein, digestible protein, total digestible nutrient(TDN), minerals(P, K, Ca, Mg), and energy(ENE, NEL, NEM, NEG) were decreased due to earlier sowing, while the content of acid detergent fiber (ADF) and neutral detergent fiber(NDF) increased. On the other hand, the yields per unit area for crude and digestible protein, TDN, minerals, and energy increased due to earlier sowing, but there was no significant difference among the plots sown on Oct. 5 through Oct. 25. The highest yield of crude protein was shown at late flowering stage, digestible protein and net energy gain (NEG) at heading stage and TDN at milk stage respectively.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.62 no.1
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• pp.24-31
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• 2017

We screened the drought tolerant maize using seventeen maize genotypes from different sources, nine inbred genotypes from United States Department of Agriculture (USDA) (B73, CML103, CML228, CML277, CML322, CML69, Ki3, Ki11, and NC350), three Southeast Asian genotypes (DK9955, LVN-4, and 333), and five Korean hybrids (Cheongdaok, Gangdaok, Ilmichal, Kwangpyeongok, and Pyeonganok). We evaluated anthesis-silking interval (ASI), leaf senescence (LS), ears per plant (EPP), tassel length (TL), and fresh weight (FW) at silking date. According to ASI and LS examination, CML103 and Kill were drought tolerant genotypes, wheareas Ki3 and 333 were drought susceptible. EPP, TL, and FW differed according to drought resistance. Grain yield was correlated strongly with ASI, but moderately with LS. Difference in ASI between drought-stressed (DS) and well-watered (WW) conditions was less than three days in CML228, CML103, Cheongdaok, NC350, B73, Ki11, CML322, and Kwangpyeongok, whereas that of Ki3, Pyeonganok, and Gangdaok was more than 6.5 days. We concluded that CML228, CML103, Cheongdaok, NC350, B73, Ki11, CML322, and Kwangpyeongok are drought tolerant genotypes, whereas Ki3, Pyeonganok, and Gangdaok are drought susceptible.

• Journal of Microbiology and Biotechnology
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• v.17 no.1
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• pp.96-103
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• 2007

Plant growth-promoting rhizobacteria (PGPR) have the potential to be used as microbial inoculants to reduce disease incidence and severity and to increase crop yield. Some of the PGPR have been reported to be able to enter plant tissues and establish endophytic populations. Here, we demonstrated an approach to screen bacterial endophytes that have the capacity to promote the growth of pepper seedlings and protect pepper plants against a bacterial pathogen. Initially, out of 150 bacterial isolates collected from healthy stems of peppers cultivated in the Chungcheong and Gyeongsang provinces of Korea, 23 putative endophytic isolates that were considered to be predominating and representative of each pepper sample were selected. By phenotypic characterization and partial 16S rDNA sequence analysis, the isolates were identified as species of Ochrobacterium, Pantoea, Pseudomonas, Sphingomonas, Janthinobacterium, Ralstonia, Arthrobacter, Clavibacter, Sporosarcina, Acidovorax, and Brevundimonas. Among them, two isolates, PS4 and PS27, were selected because they showed consistent colonizing capacity in pepper stems at the levels of $10^6-10^7CFU/g$ tissue, and were found to be most closely related to Pseudomonas rhodesiae and Pantoea ananatis, respectively, by additional analyses of their entire 16S rDNA sequences. Drenching application of the two strains on the pepper seedlings promoted significant growth of peppers, enhancing their root fresh weight by 73.9% and 41.5%, respectively. The two strains also elicited induced systemic resistance of plants against Xanthomonas axonopodis pv. vesicatoria.

• Korean Journal of Environmental Biology
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• v.21 no.4
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• pp.368-376
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• 2003

The UV-B sensitivity was tested for the intertidal species Chondrus ocellatus from Korea, by measuring photosynthesis estimated as effective quantum yield ($\Phi_{PSII}$) of photosystem II (PS II), growth and content and composition of photosynthetic pigments and UV-absorbing pigments (UVAPs). The $$\Phi_{PSII}$ of the alga decreased with increasing time of exposure to UV-B radiation, followed by fast and nearly full recovery indicating dynamic photoinhibiton. Fresh weight-based growth and pigment contents of C. ocellatus were not seriously affected by UV-B radiation. A single broad peak at 327 nm was obtained from methanol extracts of C. ocellatus, and the absorbance peak increased with increasing UV. The single peak was resolved into three peaks (311, 330 and 336 nm) by the fourth -derivative, and quantitative change in response to UV-B radiation occurred only at 330 nm. High performance liquid chromatography (HPLC) analysis of purified extracts indicated that three MAAs (mycosporine-like amino acids) are present, asterina 330, palythine and shinorine. Field observations during three growing months showed that C. ocellatus exhibit the highest amount of UVAPs in May followed by July and little trace in September, coinciding with the species' phenology. In an ecological context, dynamic photoinhibition as well as accumulation of UVAPs may enable the shallow water red alga C. ocellatus to be well adapted to high UV-B environments.

• Journal of Bio-Environment Control
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• v.16 no.3
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• pp.194-198
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• 2007

This study was performed to determine the optimum concentration of potassium for the foliar application, and its application stage to increase the production of marketable sized tomato. For this purpose, 0.25, 0.5 and 1.0 percent of potassium phosphate $(KH_2PO_4)$ were applied in the leaves of tomatoes by the stage of flowering, fruit setting and fruit development. The control plants were applied with distilled water. The results showed that foliar application with 0.5 percent of potassium phosphate increased the proportion of medium sized fruit of the products by 29.5 percent compared to the controlled plants, which is highest in the treatments. The foliar application of 0.5 percent of potassium phosphate at the fruit setting stage increased the yield of marketable sized fruit by 5.9 percent compared to the controlled plants.

• Asian-Australasian Journal of Animal Sciences
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• v.12 no.8
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• pp.1246-1250
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• 1999

Rabbits (48) of Soviet chinchilla (24) and White giant (24) were fed from 6 weeks to 12 weeks of age intensively on either of four isonitrogenous - isocaloric diets containing 0 ($D_1$), 5($D_2$), 10($D_3$) and 20($D_4$) percent raw neem seed kernel cake (NSKC), respectively as per NRC (1977) requirements in a Randomized block design and slaughtered at the end to find out differences in their carcass traits due to NSKC feeding. Dietary treatment had no significant effect on weight of edibles and inedibles and their percentages and dressing percentage in terms of carcass, carcass with pluck and carcass with pluck and head. Similarly, the meat-bone ratio of various primal cuts and overall carcass, yield of edibles per unit of inedibles and eye muscle area were not influenced due to the dietary variations. Chemical composition of fresh meat, and organoleptic evaluation of cooked meat with and without salt did not vary significantly due to incorporation of NSKC in the diets. The rabbits fed 20% NSKC ($D_4$) though consumed more (p<0.05) DM and DE per kg meat production, the intake of crude protein and total digestible nutrients was similar with other dietary treatments. Feed cost per unit meat production was, however, lower on 5 and 10% NSKC containing diets by 7.75 and 12.56%, respectively, as compared to deoiled ground nut cake containing control diet. It appears that NSKC could be used as a wholesome vegetable protein supplement upto 10% in diet of rabbits without any adverse effect on commercial carcass traits.

• Current Research on Agriculture and Life Sciences
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• v.15
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• pp.101-107
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• 1997

Bioenzyme was made up of two predominant strain, which were nomenclated PN-1 and PN-2, respectively. PN-1 possessed spore forming ability, motility, gram positive, catalase activity, but didn't have oxidase activity, urease activity, nitrate reduction and indole forming ability. Taxonomical characteristics of the PN-2 were similar to PN-1 strain, but colony color of PN-2 was yellow-white and nitrate reduction ability was positive. Predominant strains were ascertained by physiologically and morphologically. PN-1 and PN-2 were identified Bacillus lichenifonnis and Bacillus thuringinensis, respectively. Yield of lettuce significantly increased in first and second bioenzyme treatment. Fresh weight, length of shoot, width of leaves significantly increased in N,P,K and N,P,K followed by Bioenzyme application.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2018.10a
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• pp.132-132
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• 2018

The aim of this study was to enhance the Tartary buckwheat growth and phenolic profile by the application of $SiO_2$ on potsin the glasshouse. The liquid of $SiO_2$ were applied at three times after three weeks of sowing at ten days interval. The doses of the $SiO_2$ was low (2.5 ml/5 L water), high (10 ml in 5 L water) and control experiment was done without $SiO_2$. In this study, it is clearly shown that high dose of $SiO_2$ increased the buckwheat plant growth including, plant height, leaf length and width, stem diameter, fresh weight and number of seed per plant compared to control treatment. In the same way, the total phenolic compound (1421 mg/100 g), total flavonoid (35.1 mg/100g), rutin (3,130 mg/100g) and DPPH (82%) in plant were also increased high dose of $SiO_2$ compared to control (1,175 mg/100g, 31.9mg/100g, 860 mg/100g, 59%, respectively). Moreover, higher phenolic compound (1,421 mg/100g) and DPPH (82%) was observed in plant compared to seed (196 mg/100mg, 72 %, respectively). In the contrary, total flavonoid (36.2 mg/100g) and rutin (1,400 mg/100g) was higher in buckwheat seed compared to plant (35.1 mg/100g, 3,130 mg/100g, respectively). Finally, it is concluded that higher dose of $SiO_2$ enhance buckwheat growth and phenolic profile. Further investigation is needed to evaluate the optimum dose of $SiO_2$ according to soil conditions in the field.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2018.10a
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• pp.67-67
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• 2018

차요테(Sechium edule L.)는 중앙아메리카와 멕시코 남부가 원산인 박과 채소로 찜, 볶음, 튀김, 수프, 샐러드로 활용되는 아열대 채소다. 어린 순은 양념으로 무치거나 볶아서 나물로 이용되고, 열매는 샐러드, 수프, 볶음, 절임 등으로 이용된다. 동양에서는 뿌리채소로 이용되는 무(Raphanus sativus L.)와 유사하게 활용되고 있기도 하며, 인도네시아에서는 칠리 소스, 기름 등에 볶아 먹거나, 삶아서 양념을 찍어 먹기도 한다. 영양학적으로 열매에는 엽산, 비타민 C, 비타민 B6, 구리(Cu), 마그네슘(Mg) 등 미네랄이 풍부하며, 특히 칼륨(K)이 많이 함유되어 있다. 생태적으로 아열대지역에서는 다년생이지만 우리 나라에서는 일년생으로 취급하여, 봄에 정식하여 가을철에 개화되며, 늦가을에서 초겨울까지 열매를 수확한다. 본 연구는 중부지방에서 차요테 열매의 수확량이 줄어들지 않으면서, 차요테의 어린 순을 수확할 수 있는 재배기술을 개발하기 위하여, 2017년에 이어 2018년에도 동일한 처리로 시험을 수행하였다. 시설하우스에서 $2{\times}3m$ 간격으로 2018년 2월 9일부터 4월 21일까지 15일 간격으로 6회 파종하였고, 처리별로 발아시, 발아기, 발아전, 초장, 엽수, 엽록소함량, 어린순 수확량 등을 조사했다. 4차 처리구인 3월 23일 파종의 경우 발아시(최초 발아일)는 파종 후 4일이 소요되어, 1차 처리구 2월 10일 파종 보다 18일 빨랐고, 발아기(40% 발아)는 파종 후 10일로 24일 빨랐으며, 발아전(80% 발아)은 파종 후 16일로 25일 정도 더 빨리 발아되는 경향이었다. 정식 후부터 6월 하순까지 어린순 생산량은 4차 파종 시 773 g주$^{-1}$로 1차 파종 785g주$^{-1}$와 차이가 없을 정도로 생육이 좋았지만, 5차 파종 및 6차 파종의 경우 생산량이 저조하였다. 따라서, 하반기 과실 생산량을 추가적으로 조사하고, 어린순 생산량과 비교하여 중부지역 차요테의 최적 파종시기를 정할 수 있을 것으로 판단되었다.