• Korean Journal of Plant Resources
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• v.28 no.6
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• pp.697-703
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• 2015

Twenty apple germplasm accessions from the Korean Genebank were successfully cryopreserved using two-step freezing to back up genetic resources maintained by field collections. This study examined the morphological and genetic stability of cryopreserved dormant apple buds that were stored in liquid nitrogen, and then rewarmed and regrown. Whole plants were regenerated directly from dormant buds through budding without an intermediary callus phase. The cryopreserved buds produced high levels of shoot formation (76.2-100%), similar to those of noncryopreserved buds (91.3-100%), with no observed differences between cryopreserved and noncryopreserved materials. Three of the twenty cryopreserved apple germplasm accessions were used to assess morphological and genetic stability. No differences in morphological characteristics including shoot length, leaf shape, leaf width/length ratio, and root length were observed between controls (fresh control and noncryopreserved) and cryopreserved plantlets. The genetic stability of regenerants (before and after cryopreservation) was investigated using inter simple sequence repeat (ISSR) markers. The ISSR markers produced 253 bands using four primers, ISSR 810, SSR 835, ISSR 864, and ISSR 899. These markers showed monomorphic banding patterns and revealed no polymorphism between the mother plant and regenerants before and after cryopreservation, suggesting that cryopreservation using two-step freezing does not affect the genetic stability of apple germplasm. These results show that two-step freezing cryopreservation is a practical method for long-term storage of apple germplasms.

• Journal of The Korean Society of Grassland and Forage Science
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• v.21 no.3
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• pp.163-168
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• 2001

"Hwasan 103" is a new cold-tolerant Italian ryegrass(Lo1ium multiflorum L.) variety developed by theNational Livestock Research Institute(NLR1) in 1999. To develop a cold-tolerant variety of Italianryegrass(Lo1ium multiflorum L.), cold-tolerant clones survived under - 13 - - 14$^{\circ}$C of minimum average airtemperature(MAAT) in January were selected at Hwachun, Kwangwon Province in 1995. Five clones ofselected clones were polycrossed for seed production by NLRI, RDA in 1996."Hwasan 103" was diploid variety, green in leaf color and broad in flag leaf width. Also it hassemi-prostrate and medium growth habit in late autumn and in early spring, respectively. "Hwasan 103" wasexcellent in lodging tolerance at harvesting time. First heading date of "Hwasan 103" was 14th May, it wassimilar to that of Marshall. Expecially, "Hwasan 103" survived about 50-70% under - 10- - 12'C ofMAAT in January, so it could be cold-tolerant variety that can be safely cultivated in regions higher than -9$^{\circ}$C of MAAT in January. Compared with Marshall "Hwasan 103" showed 3% higher fresh yield (57.8MTper ha) and it showed 2% higher dry matter yield (11.OMT per ha). Higher IVDMD and TDN and lowerADF and NDF were observed in "Hwasan 103" than those of Florida 80 and Marshall in Forage quality.(Key words : Italian ryegrass, Cold tolerance, New variety "Hwasan 103")all in Forage quality.(Key words : Italian ryegrass, Cold tolerance, New variety "Hwasan 103")n 103&")ot;)

• Journal of the Korean Society of Environmental Restoration Technology
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• v.19 no.3
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• pp.1-9
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• 2016

The objective of this study was to exam early stages of plant growth of Euonymus fortunei 'Emerald and Gold' and Hedera japonica grown in fabric felt bag attached on vertical greenery system with the ratio of hydrophilic polymer to substrate, 0(referred as Control), 0.1, 0.2, or 0.4% (v/v), in order to effectively utilize rainfall for low maintenance. After rainfall, the increase in volumetric water content of substrate amended with the hydrophilic polymer was between 13% and 87%, compared to that in Control. During drought period, higher ratio of hydrophilic polymer to substrate slowly decreased the volumetric water content, and increased plant height, leaf length, leaf width, number of leaves, branch number, and stem diameter of both Euonymus fortunei 'Emerald and Gold' and Hedera japonica with the highest fresh and dry weights of shoots or roots under the 0.1% ratio of hydrophilic polymer.

• Korean Journal of Medicinal Crop Science
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• v.15 no.1
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• pp.56-61
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• 2007

This study was carried out to determine the antioxidant activity and vitamin C contents in plant extracts of the Momordica charantia L. The vitamin C was detected as the highest content in immature fruit (92.2 mg/100 g), while the content in stem (2.5 mg/100 g) was lower 40 times than that of immature fruit. Antioxidant activity for the dried sample was investigated by TBA method. The lowest TBARS values were obtained from extracts of dried leaf and followed by ascorbic acid and BHT, showing that the extracts from dried leaf possess the strongest antioxidant activity. Compared with fresh tissues, SOD activity, ATX activity and CAT activity were high level in the dried tissue. These results suggest that the Momordica charantia L. would be a promising antioxidant source as an alternative antioxidant, based on natural plant resources.

• Journal of The Korean Society of Grassland and Forage Science
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• v.41 no.3
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• pp.162-167
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• 2021

Aluminum (Al) stress in acidic pH is known to decrease the growth and productivity of alfalfa. However, not much is known about how the application of silicon (Si) affects the Al stress response in alfalfa. This study was conducted to evaluate the effect of exogenous application of Si on the growth of alfalfa seedlings exposed to Al stress in pots. Alfalfa seedlings grown in pots for 2 weeks were treated either Al stress (pH 4.0, 0.2 mM Al) or Al stress + Si (1 mM) for 5 days, lengths and biomass of shoot and root, and chlorophyll and carotenoid contents in leaf tissues were analyzed respectively. Al stress treatment inhibited shoot and root growth, and decreased fresh and dry weights, and chlorophyll content in leaves, but increased carotenoid content. In contrast, when alfalfa seedlings treated with Al stress combined with Si, delayed growth caused by Al stress of shoot and root of alfalfa seedlings was restored, dry weight was increased and chlorophyll content of leaf tissue was increased, but carotenoid content was decreased. These results suggest that Si has a function of alleviating Al toxicity in alfalfa, of which it exhibits a mitigating effect by a function that overlaps with some of the intracellular functions of carotenoids.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2019.04a
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• pp.55-55
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• 2019

Strawberry which is the genus Fragaria under family Rosaceae is one of the most important fruit plants for both fresh consumption and food processing in the temperate and subtropical countries. Propagation of strawberry is achieved either through runners or by in vitro micropropagation. Meristem tips, generally obtained from runners of virus-free plants, are commonly used to establish in vitro cultures, which are employed for mass propagation or as a source of plant material for regeneration and transformation experiments. This study was conducted to determine the optimal natural additives strength to improve sprouting shoot rate of apical meristem of strawberry 'Seolhyang'. Strawberry apical meristem at size (0.2 mm to 0.3 mm) with leaf primordials were cultured on the 1/3MS(Murashige & Skoog) medium supplemented with five natural additives such as coconut milk, maple sap, banana powder and peptone. The sprouting ratio and growth characteristics were evaluated after eight weeks after in vitro culture. Shoot ratio of 'Seolhyang' apical meristem was 72.9% in 1/3MS medium supplemented with maple sap. On the other hand, the low shoot ratio was observed 47.7% in 1/3MS medium supplemented with banana powder. Shoot length was different as natural additives but numbers of leaf was not significantiy different among the natural additives. As a result, the sprouting ratio and plant growth were enhanced effectively in 1/3MS medium with maple sap compared to the others.

• The Plant Pathology Journal
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• v.35 no.2
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• pp.178-187
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• 2019

Plants are exposed to biotic stresses caused by pathogen attack and complex abiotic stresses including heat and drought by dynamic climate changes. To alleviate these stresses, we investigated two bacterial stains, H26-2 and H30-3 in two cultivars ('Ryeokkwang' and 'Buram-3-ho') of Chinese cabbage in plastic pots in a greenhouse. We evaluated effects of bacterial strains on plant growth-promotion and mitigation of heat and drought stresses; the role of exopolysaccharides as one of bacterial determinants on alleviating stresses; biocontrol activity against soft rot caused by Pectobacterium carotovorum subsp. carotovorum PCC21. Strains H26-2 and H30-3 significantly increased fresh weights compared to a $MgSO_4$ solution; reduced leaf wilting and promoted recovery after re-watering under heat and drought stresses. Chinese cabbages treated with H26-2 and H30-3 increased leaf abscisic acid (ABA) content and reduced stomatal opening after stresses treatments, in addition, these strains stably colonized and maintained their populations in rhizosphere during heat and drought stresses. As well as tested bacterial cells, exopolysaccharides (EPS) of H30-3 could be one of bacterial determinants for alleviation of tested stresses in Chinese cabbages, however, the effects were different to cultivars of Chinese cabbages. In addition to bacterial activity to abiotic stresses, H30-3 could suppress incidence (%) of soft rot in 'Buram-3-ho'. The tested strains were identified as Bacillus aryabhattai H26-2 and B. siamensis H30-3 based on 16S rRNA gene sequence analysis. Taken together, H26-2 and H30-3 could be candidates for both plant growth promotion and mitigation of heat and drought stresses in Chinese cabbage.

• Journal of Bio-Environment Control
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• v.31 no.4
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• pp.286-291
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• 2022

The effect of root-zone local cooling on seedling growth of tomato was investigated. Lower pipe cooling was used for local cooling of the root zone, and the root zone temperature was set at 20 and 25℃. There was no difference in plant height, root length, and leaf number according to local cooling temperature. Leaf area, fresh weight, dry weight, and chlorophyll content of the shoot and root was higher in the 25℃ than those of 20℃ at 28 DAS. These results showed that cooling for seedling growth of tomato 25℃ is sufficient considering energy efficiency. This study will be helpful in the development of local cooling technology that can reduce the energy required for cooling during the production of tomato seedlings in the high temperature season.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.25 no.2
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• pp.15-22
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• 2022

This study has been conducted to investigate the appropriate mixed sowing rates with white clover (Trifolium repens) and Kentucky bluegrass (Poa pratensis) by evaluating initial growth. Six different mixed sowing rates (CT, Control Trifolium repens; CP, Control Poa pratensis; T1P3, Trifolium repens 1 : Poa pratensis 3; T1P2, Trifolium repens 1 : Poa pratensis 2; T1P1, Trifolium repens 1 : Poa pratensis 1; T2P1, Trifolium repens 2 : Poa pratensis 1; T3P1, Trifolium repens 3 : Poa pratensis 1) with three repetitions were implemented in greenhouse experiment plastic pots (50.5 length × 35.0 width × 8.5 cm height). Plant height, leaf length, leaf width, number of leaves, root length, fresh weight, and dry weight were measured after a week from the germination for three months. Initial growth of Kentucky bluegrass significantly increased as mixed sowing rates with increasing, while that of white clover kept relatively decreasing than that in Control. Initial growth strongly affected sowing mixed rates by Kentucky bluegrass and not by white clover in this experiment, indicating that grasses played a dominant role during initial growth period. However, seeding rates with white clover will be dependent on the use of a turf and the desired green cover. There remains a need to further study of the mechanisms and dynamics of legume and grass competition to underpin sustainable management practices.

• Korean Journal of Agricultural Science
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• v.49 no.3
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• pp.643-653
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• 2022

This study investigated which nutrient solution is suitable for growth and secondary metabolite contents when three different vegetable plants are grown simultaneously in one hydroponic cultivation bed. Seeds of pak choi (Brassica compestris L. ssp chinsensis), red mustard (Brassica juncea L.), and arugula (Eruca sativa Mill.) were sown in the shape of a triangle in three places on rockwool cubes. The rockwool cubes were placed in semi deepflow technique (semi-DFT) hydroponic systems in a rooftop greenhouse after three weeks of growth as seedlings then cultivated with four different nutrient solutions, Korea Horticultural Experiment Station (KHE), Hoagland, Otsuka-A, and Yamazaki, at the rooftop greenhouse for two weeks. The leaf area of pak choi cultivated in Otsuka-A was the largest but SPAD values, leaf area, and fresh weight of arugula were highest with KHE treatment. The total glucosinolate (GSL) content of pak choi was 151.7% higher in KHE than in Hoagland, and there was no significant difference in Yamazaki and Otsuka-A treatments. The total GSL content of red mustard was 34.6 μmol·g^-1 in Hoagland, and it was 32.6% higher in Hoagland than in Yamazaki. Total GSL content of arugula was 57.5% higher in Yamazaki and Hoagland nutrients than in KHE and Otsuka-A nutrients solutions. The total GSL content of three plants grown with KHE was 40.7% higher than with Yamazaki, and the other nutrient solutions did not show significant differences. Therefore, KHE nutrient solution is considered suitable for nutrient solution composition for the cultivation of three different Brassicaceae crops in a single hydroponic cultivation system.