Browse > Article

Antioxidant Activity of Momordica charantia L. Extracts  

Park, Yeol (Department of Biotechnology, Collage of Nature Science, Chosun University)
Boo, Hee-Ock (Department of Biology, Collage of Nature Science, Chosun University)
Park, Young-Lan (Department of Anatomy, Collage of Medicine, Chosun University)
Cho, Dong-Ha (School of Bioscience & Biotechnology, Kangwon National University)
Lee, Hyun-Hwa (Department of Biology, Collage of Nature Science, Chosun University)
Publication Information
Korean Journal of Medicinal Crop Science / v.15, no.1, 2007 , pp. 56-61 More about this Journal
Abstract
This study was carried out to determine the antioxidant activity and vitamin C contents in plant extracts of the Momordica charantia L. The vitamin C was detected as the highest content in immature fruit (92.2 mg/100 g), while the content in stem (2.5 mg/100 g) was lower 40 times than that of immature fruit. Antioxidant activity for the dried sample was investigated by TBA method. The lowest TBARS values were obtained from extracts of dried leaf and followed by ascorbic acid and BHT, showing that the extracts from dried leaf possess the strongest antioxidant activity. Compared with fresh tissues, SOD activity, ATX activity and CAT activity were high level in the dried tissue. These results suggest that the Momordica charantia L. would be a promising antioxidant source as an alternative antioxidant, based on natural plant resources.
Keywords
Momordica charantia L.; vitamin C; TBA-reactive substance; superoxide dismutase; ascorbic peroxidase; catalase;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Schmourlo G, Mendonca-Filho RR, Alviano CS, Costa SS (2005) Screening of antifungal agents using ethanol precipitation and bioautography of medicinal and food plants. Journal of Ethnopharmacology 96(3):563-568   DOI   ScienceOn
2 Wheeler GL, Jones MA, Smirnoff N (1998) The biosynthetic pathway of vitamin C in higher plants. Nature 393(6683):365-369   DOI   ScienceOn
3 Alscher RG, Hess JL (1993) Antioxidants in higher plants. CRC Press, Boca Raton, 1-174
4 Asada K (1999) The water-water cycle in chloroplasts: Scavenging of active oxygen and dissipation of excess photons. Annu. Rev. Plant Physiol. Plant Mol. Biol. 50:601-639   DOI
5 Bourinbaiar AS, Lee-Huang S (1995a) Acrosin inhibitor, 4'-acetamidophenyl 4-guanidinobenzoate, an experimental vaginal contraceptive with anti-HIV activity. Contraception 51(5):319-32   DOI   ScienceOn
6 Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72:248-254   DOI   ScienceOn
7 Chon SU, Kim, YM, Han SK, Choi SK (2004) Antioxidative Fig. 4. CAT activity in dry sample (A) and fresh sample (B) in Momordica charandia L. effects of several compositae plants. Kor. J. Plant Res. 17(1):14-19
8 Nakano Y, Asada K (1981) Hydrogen peroxide os scavenged by ascorbate-specific peroxidase in spinach chloroplast. Pland Cell Physiol. 22:867-880
9 Park YJ, Kang M., Kim JI, Park OJ, Lee MS, Jang DJ (1995) Changes of Vitamin C and Superoxide Dismutase(SOD)-like Activity of Persimmon Leaf Tea by Processing Method and Extraction Condition. Kor. J. Food Sci. Technol. 27(3):281-285
10 Rathi SS, Grover JK, Vats V (2002) The effect of Momordica charantia and Mucuna pruriens in experimental diabetes and their effect on key metabolic enzymes involved in carbohydrate metabolism. Phytotherapy Research 16(3):236-243   DOI   ScienceOn
11 Parkash A, Ng TB, Tso WW (2002) Purification and characterization of charantin, a napin-like ribosome-inactivating peptide from bitter gourd (Momordica charantia) seeds. Journal of Peptide Research 59(5):197-202   DOI   ScienceOn
12 Beloin N, Gbeassor M, Akpagana K, Hudson, J, De Soussa K, Koumaglo K, Arnason JT (2005) Ethnomedicinal uses of Momordica charantia (Cucurbitaceae) in Togo and relation to its phytochemistry and biological activity. Journal of Ethnopharmacology 96(1-2):49-55   DOI   ScienceOn
13 Feri D, Ames BN (1991) Ascorbic acids as protects plasma lipids against oxidative damage. Nutrition and Cancer 15:250-252
14 Beauchamp C, Fridovich J (1971) Superoxide dismutase: Improved assays and an assay applicable to acrylamide gels. Anal. Biochem. 44:276-287   DOI   ScienceOn
15 Zong RJ, Morris L, Cantwell M (1995) Postharvest physiology and quality of bitter melon (Momordica charantia L.). Postharvest Biology and Technology 6(1-2):65-72   DOI   ScienceOn
16 Aebi HE (1983) Catalase: in methodes of enzymatic analyses (Bergmeyer, H.U. ed.). Verlag Chemie. Weinheim. 3:273-282
17 Bowler C, Van Montagu M, Inze D (1992) Superoxide dismutases and stress tolerance. Annu. Rev. Plant Physiol. Plant Mol. Biol. 43:83-116   DOI
18 Park PS (1989) Investigation of Vitamin C in the Lines of Bitter Gourd(Momordica Charantia L.) J. Chinju Nat. Agri. & For. Jr. Coll. 24:235-238
19 Witte VC, Krause ME, Bailey T (1970) A new extraction methord for determining 2-thiobarbituric acid values of pork and beef during storage. J. Food Sci. 35:582-587   DOI
20 Virdi J, Sivakami S, Shahani S, Suthar AC, Banavalikar MM, Biyani MK (2003) Antihyperglycemic effects of three extracts from Momordica charantia. Journal of Ethnopharmacology 88(1):107-111   DOI   ScienceOn
21 Ji LL, Fu R, Mitchell EW (1992) Glutathione and antioxidant enzymes in skeletal muscle: effect of fiber type and exercise intensity. J. Applied Physiology 73(5):1854-1859   DOI
22 Grover JK, Yadav SP (2004) Pharmacological actions and potential uses of Momordica charantia: a review. Journal of Ethnopharmacology 93(1):123-132   DOI   ScienceOn
23 Allen RD, Webb RP, Schake SL (1997) Use of transgenic plants to study antioxidants defense. Free Rad. Biol. Med. 23:473-479   DOI   ScienceOn
24 Heath RL (1987) The biochemistry of ozone attack on the plasma membrane of plant cells. Adv. Phytochem. 21:29-54
25 Bourinbaiar AS, Lee-Huang S (1995b) Potentiation of anti-HIV activity of anti-inflammatory drugs, dexamethasone and indomethacin, by MAP30, the antiviral agent from bitter melon. Biochemical & Biophysical Research Communications 208(2):779-785   DOI   ScienceOn
26 Kang NJ, Kwon JG, Lee HC, Jeong HB, Kim, HT (2003) Antioxidant Enzymes as Defense Mechanism against Oxidative Stress Induced by Chilling in Cucurbita ficifolia Leaves. J. Kor. Sod. Sci. 44(5):605-610
27 Jakeman MJ, Edwards RH, Symonns MC (1993) Electron spin resonance studies of intact mammalian skeletal muscle. Biochem. Biophy. Acta. 874(2):185-190
28 Giron LM, Freire V, Alonzo A, Caceres A (1991) Ethnobotanical survey of the medicinal flora used by the Caribs of Guatemala. Journal of Ethnopharmacology 34(2-3):173-187   DOI
29 Hamato N, Koshiba T, Pham TN, Tatsumi Y, Nakamura D, Takano R, Hayashi K, Hong YM, Hara S (1995) Trypsin and elastase inhibitors from bitter gourd (Momordica charantia LINN.) seeds: purification, amino acid sequences, and inhibitory activities of four new inhibitors. Journal of Biochemistry 117(2):432-437   DOI