• Asian-Australasian Journal of Animal Sciences
• /
• v.16 no.8
• /
• pp.1102-1107
• /
• 2003

The ES cell can provide a useful system for studying differentiation and development in vitro and a powerful tool for producing transgenic animalds. To investigate the culture condition of chicken embryonic stem (CES) cells which can retain their multipotentiality or totipotency, three kinds of feeder layer cells, SNL cells, primary mice embryonic fibroblasts (PMEF) cells and primary chicken embryonic fibroblasts (PCEF) cells, were used as the feeder cells in media of DMEM supplemented with leukemia inhibitory factor (LIF), basic fibroblast growth factor (bFGF) and stem cell factor (SCF) for co-culture with blastoderm cells from stage X embryos of chicken. The alkaline phosphatase (AKP) test, differentiation experiment in vitro and chimeric chicken production were carried out. The results showed that culture on feeder layer of PMEF yielded high quality CES cell colonies. The typical CES cells clone shape revealed as follows: nested aggregation (clone) with clear edge and round surface as well as close arrangement within the clone. Strong alkaline phosphatase (AKP) reactive cells were observed in the fourth passage cells. On the other hand, the fourth passage CES cells could differentiate into various cells in the absence of feeder layer cells and LIF in vitro. The third and fourth passage cells were injected into the subgerminal cavity of recipient embryos at stage X. Of 269 Hailan embryos injected with CES cells of Shouguang Chickens, 8.2% (22/269) survived to hatching, 5 feather chimeras had been produced. This suggests that an effective culture system established in this study can promote the growth of CES cells and maintain them in the state of undifferentiated and development, which lays a solid foundation for the application of CES cells and may provide an alternative tool for genetic modification of chickens.

• Structural Monitoring and Maintenance
• /
• v.6 no.4
• /
• pp.317-346
• /
• 2019

Performance assessment of pavements proves useful, in terms of handling the ride quality, controlling the travel time of vehicles and adequate maintenance of pavements. Roughness profiles provide a good measure of the deteriorating condition of the pavement. For the accurate estimates of pavement roughness from dynamic vehicle responses, vehicle parameters should be known accurately. Information on vehicle parameters is uncertain, due to the wear and tear over time. Hence, condition monitoring of pavement requires the identification of pavement roughness along with vehicle parameters. The present study proposes a scheme which estimates the roughness profile of the pavement with the use of accurate estimates of vehicle parameters computed in parallel. Pavement model used in this study is a two-layer Euler-Bernoulli beam resting on a nonlinear Pasternak foundation. The asphalt topping of the pavement in the top layer is modeled as viscoelastic, and the base course bottom layer is modeled as elastic. The viscoelastic response of the top layer is modeled with the help of the Burgers model. The vehicle model considered in this study is a half car model, fitted with accelerometers at specified points. The identification of the coupled system of vehicle-pavement interaction employs a coupled scheme of an unbiased minimum variance estimator and an optimization scheme. The partitioning of observed noisy quantities to be used in the two schemes is investigated in detail before the analysis. The unbiased minimum variance estimator (MVE) make use of a linear state-space formulation including roughness, to overcome the linearization difficulties as in conventional nonlinear filters. MVE gives estimates for the unknown input and fed into the optimization scheme to yield estimates of vehicle parameters. The issue of ill-posedness of the problem is dealt with by introducing a regularization equivalent term in the objective function, specifically where a large number of parameters are to be estimated. Effect of different objective functions is also studied. The outcome of this research is an overall measure of pavement condition.

• International Journal of Internet, Broadcasting and Communication
• /
• v.16 no.1
• /
• pp.241-252
• /
• 2024

We analyze the types of Web3 business model innovation (BMI) of the six major Korean game companies by market size. As a result of the analysis, Nexon is watched as the adapter. It introduces blockchain (BC) layer 2, 'Polygon' to the extended ecosystem such as the creator's secondary creation, item utility, and compensation experience using the existing core intellectual property (IP). KakaoGames and Neowiz are watched as the adventurers. KakaoGames introduces BC layer 2, 'Polygon' and 'Near Protocol' to various experiments using tokenomics models in casual games and massively multiplayer online role-playing games (MMORPGs) using several existing popular IPs. Neowiz also introduces BC layer 2, 'Polygon' and 'Avalanche' to the IntellaX platform using existing game IPs. As the reinventor, Netmable positions as a game publisher that releases third-party games based on multi-chain infrastructure such as Klaytn, BNB Chain, Near Protocol, Aptos Foundation, and introduces BC to new core IPs. Finally, there are Wemade and Com2us as the mavericks. They aim to be the Web3 platform operators that create a BC layer 1 ecosystem and provide services that encompass BC games, GameFi, and non-fungible tokens (NFTs). Here are the implications of the four types of BMI. In terms of infrastructure, Nexon, KakaoGames, and Neowiz try to introduce a part of cross-chain, whereas Netmable tries to move toward a complete multi-chain strategy, and Wemade and Com2us also try to consider multi-chain, even if they have the full BC introduction. In terms of defending against market decline, Nexon and Netmable have a different position. Nexon which has a greater market dominance, only tries to continuously experiment, but Netmable is aggressively focusing on monetizing new products. Attacks on growth aspirations also show two different positions. KakaoGames and Neowiz only try to aggressively explore, while WeMade and Com2us try to set new standards for industrial innovation.

• Economic and Environmental Geology
• /
• v.55 no.6
• /
• pp.583-596
• /
• 2022

The rock surface of Bangudae petroglyphs is mainly dark brown hornfelsified shales by contact metamorphism. The surface form a weathered layer of a invariable depth, and there is a difference with mineral and chemical composition between weathered and non-weathered layers. Surface of the petroglyphs has been discolored to light brown over the face due to biological and chemical weathering. As the measuring chromaticity based on the non-weathered layer, the whiteness and yellowness increased in the weathered layer, and the color difference (ΔE) was 5.54 to 36.89 (mean 17.26). In the weathered layer of the petroglyph surface, the CaO content was reduced by about 90% compared to the non-weathered layer, and Sr also showed the same trend. In particular, the mean porosity of the non-weathered layer was 0.4%, but it was estimated as 25.0% in the weathered layer. This is interpreted as the fact that calcite reacts with water, and forms a weathered layer from the surface as it is eluted. Based on the weathering depth modeling of the petroglyphs using the penetration characteristics of X-rays, the weathering depth of rock faces was found to be 1 to 2mm. However, the area classified as 2mm or more estimated to be a maximum of 3 to 4mm, considering the weathering depth around the petroglyphs surface.

• Geotechnical Engineering
• /
• v.1 no.2
• /
• pp.81-92
• /
• 1985

Braced excavation for a new building was carried out at a very close proximity of an existing tall building of T.hick columns are supported by indict.ideal spread footings on sand layer The excavation was planned to reach far below the footing level of the existing building. To assess the foundation performance and stability of the existing building, the behavior of 9round subjected to loss of confinement from excavation was analytically studied using finite element method. Field instrumentation was also conducted to monitor the actual ground responses during excavation. Based on these studies, various remedial measures weere taken to minimize the adverse effects to the building, and excavation was successfully completed. This paper presents the results from the analytical studies and field monitoring, and measured and measured responses at different stages of excavation.

• Journal of Environmental Science International
• /
• v.17 no.11
• /
• pp.1235-1241
• /
• 2008

Membrane separation is extensively used for water/wastewater treatment because of its efficiency separation processes. However, particles in the feed water can deposit and accumulate on the membrane surface to created cake layer. As a consequence, the selectivity of the membrane and flux through the membrane are decreased, which is called fouling/blocking phenomenon. In order to solve fouling problem, we developed a novel membrane named Carbon Whisker Membrane (CWM) which contains vapor-grown carbon fibers/whiskers on the surface of the membrane and a layer of carbon film coated on the ceramic substrate. We firstly employed polymethyl methacrylate (PMMA) as a testing material to investigate the fouling mechanism. The results suggested that Carbon Whiskers on the surface of the membrane can prevent the directly contact between the membrane body and particles so that the fouling/blocking could not occurred easily compared to the membrane without carbon whiskers. We also researched the relationship with the diameter, density of carbon whisker on the membrane surface and total flux of solutions. Finally, we will be able to control the diameter and density of carbon whiskers on the membrane and existence of carbon whiskers on the membrane, it is important factor, might be prevent fouling/blocking in the water treatment.

• Structural Engineering and Mechanics
• /
• v.61 no.6
• /
• pp.775-784
• /
• 2017

This study is devoted to developing many new substructure models for ballasted railway track by using the pyramid model philosophy. As the effect of railway embankment has been less considered in the previous studies in the field of vehicle/track interaction, so the present study develops the pyramid models in the presence of railway embankment and implements them in vehicle/track interaction dynamic analyses. Considering a moving car body as multi bodies with 10 degrees of freedom and the ballasted track including rail, sleeper, ballast, subgrade and embankment, two categories of numerical analyses are performed by considering the new substructure systems including type A (initiation of stress overlap areas in adjacent sleepers from the ballast layer) or type B (initiation of stress overlap areas in adjacent sleepers from the subgrade layer). A comprehensive sensitivity analyses are performed on effective parameters such as ballast height, sleepers spacing and sleeper width. The results indicate that the stiffness of subgrade, embankment and foundation increased by increasing the ballast height. Also, by increasing the ballast height, rail and ballast vertical displacement decreased.

• Journal of the Korean Institute of Illuminating and Electrical Installation Engineers
• /
• v.26 no.9
• /
• pp.57-63
• /
• 2012

The basic purpose of grounding is for human safety and normal operation of system related to electrical shock hazard by faults of electrical equipments. A grounding electrode is defined as a conducting element that connects electrical systems and/or equipment to the earth. The lowest possible resistance connection to the earth is sought from the grounding electrode. The grounding electrode is the foundation of the electrical safety system. The resistance to ground of vertical electrodes buried in the two deference soil structures has been analyzed for a length of electrodes and soil parameters. The equation of ground resistance of vertical electrodes are Tagg's equation for uniform soil models, and modified equation of Dwight equation for two-layer soil model. In this paper, compared with results of two equations are calculated values of vertical electrode in uniform and two-layer soil models.

• Proceedings of the Korea Association of Crystal Growth Conference
• /
• 1996.06a
• /
• pp.139-156
• /
• 1996

The phase field model is becoming the model of choice for the theoretical study of the morphologies of crystals growth from the melt. This model provides an alternative approach to the solution of the classical (sharp interface) model of solidification by introducing a new variable, the phase field, Ø, to identify the phase. The variable Ø takes on constant values in the bulk phases and makes a continuous transition between these values over a thin transition layer that plays the role of the classically sharp interface. This results in Ø being governed by a new partial differential equation(in addition to the PDE's that govern the classical fields, such as temperature and composition) that guarantees (in the asymptotic limit of a suitably thin transition layer) that the appropriate boundary conditions at the crystal-melt interface are satisfied. Thus, one can proceed to solve coupled PDE's without the necessity of explicitly tracking the interface (free boundary) that would be necessary to solve the classical (sharp interface) model. Recent advances in supercomputing and algorithms now enable generation of interesting and valuable results that display most of the fundamental solidification phenomena and processes that are observed experimentally. These include morphological instability, solute trapping, cellular growth, dendritic growth (with anisotropic sidebranching, tip splitting, and coupling to periodic forcing), coarsening, recalescence, eutectic growth, faceting, and texture development. This talk will focus on the fundamental basis of the phase field model in terms of irreversible thermodynamics as well as it computational limitations and prognosis for future improvement. This work is supported by the National Science Foundation under grant DMR 9211276

• Journal of the Korean Society for Precision Engineering
• /
• v.32 no.9
• /
• pp.787-792
• /
• 2015

Recently, 3D printing technology is a hot issue in various industrial fields. According to the user's application, it allows for the free form fabrication method to be utilized in a wide range. The powder based fusion technique is one of the 3D printing methods. When using this method it is possible to apply the various binder jetting techniques such as piezo, thermal bubble jet, dispenser and so on. In this paper, a multi thermal bubble ink jet was integrated for jetting of powder binding material and developing a power fused 3D printing system. For high quality 3D printing parts, it needs an analysis and evaluation of the behavior of the thermal bubble ink jet head. In the experiment, a correlation between jetting binder quantity and layer thickness of powder was investigated, and a 3D part model was fabricated, which was used by measuring the scale factor.