• 한국식품과학회지
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• 제31권6호
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• pp.1628-1634
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• 1999

Listeria monocytogenes의 식품 속에서 신속하고 특이적인 검출을 위하여, listeriolysin O gene에 의한 primer, LM 1과 LM 2를 선택하여 PCR을 수행하였다. L. monocytogenes의 DNA 추출이나 cell lysis 없이 intact whole cell을 직접 이용하여 PCR을 하였으며 $10^{2-6}$ CFU 수준의 균체 배양액으로부터 L. monocytogenes에 특이적인 702 bp의 PCR 증폭 산물을 확인하였다. 우유, 닭고기, 김치 등의 식품에 L. monocytogenes를 접종하여 증균배양 전후 균의 PCR에 대한 감도와 생균수를 비교한 결과, 실험된 식품 속에서의 L. monocytogenes의 검출 감도는 순수 배양액에서의 경우에 비해 약 1/10로 둔화되었으나 역시 특이적인 검출이 가능하였다. Primer LM 1과 2를 이용한 본 실험조건에서의 L. monocytogenes의 PCR에 의한 검출은 약 4시간으로 확인이 가능하였으며, 기존의 배양 방법에 비해, 특이성이나 검출 속도 면에서 식품위생 실무에 적용하기 위한 높은 잠재력을 보여 주었다.

• Journal of Nutrition and Health
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• 제37권7호
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• pp.550-556
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• 2004

Dietary pattern analysis is important complementary approach for identifying associations between diet and chronic disease. A case-control study was conducted in order to examine dietary patterns and the risk of colon cancer in Korea. Data were collected from both 137 cases with either colorectal cancer or large bowl adenomatous polyps and 134 controls regarding social-demographic characteristics and food intake using a semi-quantitative food frequency questionnaire. We conducted factor analysis and identified 6 major dietary patterns: 'Well-being diet' characterized by higher intakes of potatoes, yogurt, soybean paste and vegetables, 'Meat & fish', 'Milk & juice', 'Pork & alcohol', 'Rice & kimchi', and 'Coffee & cake'. We calculated factor scores for each participant and examined the associations between dietary patterns and colon cancer risk. After adjusting for potential confounders, there was a relative risk for colon cancer of 0.16 (95% confidence interval, 0.07 - 0.34) when comparing the highest with the lowest tertile of the 'Well-being' pattern. Significant trends of decreasing risk of colon cancer also emerged with the 'Milk & juice' (OR = 0.40, 95% CI = 0.20 - 0.79). In contrast, inverse associations of the risk were found for 'Pork & alcohol' (OR = 1.92, 95% CI = 0.93 - 3.97), 'Coffee & cake' (OR = 2.18, 95% CI = 1.07 - 4.46). For the 'Meat & fish' pattern, the decreased risk of colon cancer was observed in the second tertile, but not in the highest tertile when comparing to the lowest. The 'Rice & kimchi' pattern had a nonsignificant association with the risk. These data suggest that major dietary patterns derived from the FFQ associated with the risk of colon cancer in Korea. Since foods are not consumed in isolation, dietary pattern research in natural eating behavior may be useful for understanding dietary causes of colon cancer.

• Journal of Nutrition and Health
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• 제30권1호
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• pp.61-74
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• 1997

A dietary intake survey in 2 major city area was conducted as a part of the study which determined the level of exposure of people to harmful substances in foodstuff. A total of 800 randomly selected men and women aged 20-49 yr and living in the cities of Seoul and Taegu were invited to participate in the survey. All subjects were interviewed by trained dietitians to answer for demorgraphic characteristics and dietary intake for 1 day by 24hr recall method, and asked to record what day ate for following 2 more days by themselves. Results were analyzed and presented for the amount and frequency of food intake by area, sex body weight. Food consumed most by adults resifing in these 2 city area was rice and followed by kimchi in the amount of 225.7g/persn/day and 85.0g/person/day, respectively. In the list of foods consumed most frequently, items used for condiments were included in addition to rice and kimchi. And the total daily intake of food per person was summed up to be 1, 534g for men and 1, 304g for women. Considerable part of this sexual difference in total intake was originated from the difference in beverage consumption including alcoholic beverages and the higher intake of certain foodstuff due to higher body weight of men. Intake data were presented din terms of per capita and per kg body weight, and compared among adults in different categories of body weight, also. The data gathered here, food intake by individuals, reflect our own dietary culture in terms of food assortment and relative contribution of each food item to total food intake include the consumption of "extreme" eaters might be found useful also especially for the sake of food safety such as risk assessment using ADI and PTWI for contaminants in the food supply.od supply.

• Journal of Nutrition and Health
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• 제36권6호
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• pp.622-634
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• 2003

The purpose of this study was to investigate the dietary habits and altered food preferences of stroke patients. One hundred and forty-six outpatients, who had experienced their first-ever stroke and were admitted to Asan Medical Center between July and December 2000, were studied. Using interviews, we assessed the altered food preferences, food consumption frequency, and other factors influencing the food preferences and food consumption frequency of the subjects. These results were analyzed with 2 t-tests, and multiple regression analysis, using the SPSS package program. Preferences for pork, red fish, coffee, bread and stews were higher in male stroke patients than in females. The frequency of consumption of beef, pork, white fish, red fish, egg, garlic, onion, coffee, instant noodles, bread, and culinary vegetables increased in the male stroke patients more than in the females. Food preferences were influenced by income, risk factors, subjective tastes and location of brain ischemic lesions. Food consumption frequency was affected by food preference, income, drugs, alcohol, marital status, sex, and dysgeusia. As a result of multiple regression analysis, the frequency of consumption of white fish, red fish, eggs, soy milk, milk, garlic, onions, coffee, noodles, bread, bean-paste stew, kimchi, culinary vegetables, and greasy foods were the most affected by each food preference. Our results suggest that food consumption frequency may vary with food preference, income, drugs, alcohol, marital status, sex, and dysgeusia, and nutrition education should be formulated to prevent stroke recurrence based on the food preferences, subjective tastes, and risk factors of individual stroke patients.

• 한국식품영양과학회지
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• 제28권6호
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• pp.1380-1390
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• 1999

To assess the food intake and diet quality of preschool children in Pusan, dietary survey was conducted with 176 subjects using 24hr recall method. Diet quality was assessed by food group pattern, dietary diversity score(DDS), dietary variety score(DVS), and dietary frequency score(DFS). The total number of food items consumed was 307. The foods consumed most frequently were rice, Korean cabbage kimchi, milk and green onion. When investigating the consumption pattern of major six food groups, 38.1% of subjects consumed all six groups. Food group was missed in order of fruit(41.5%), sweet(19.9%), dairy(16.5), vegetable(4.5%) and meat(1.7%). The mean values of DDS, DVS, and DFS were 5.16, 13.82, and 16.07 re spectively. Persons who had higher DDSs also had higher DVSs(p<0.001). DDS, DVS and DFS correlated positively with NAR(nutrient adequacy ratio) and MAR(mean adequacy ratio) significantly. Associations between the NAR of most nutrients and MAR with DVS and DFS were higher than those between the NAR of most nutrients and MAR with DDS. People with DDS of above 6 or DVS of above 16 or DFS of above 21 met two thirds of recommended dietary allowance for nutrients. Based on the result of the food group intake, the food intake of subjects was not adequate, especially the fruit and dairy groups. When assessing the dietary quality of subjects using DDS, DVS and DFS, many people appeared not to have a desirable food intake.

• Journal of Nutrition and Health
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• 제53권6호
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• pp.663-675
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• 2020

본 연구에서는 서울시내 일부 가정식, 외식, 학교급식에서 국물음식을 수집하여 건더기와 국물 각각의 나트륨 함량을 분석하고 제공 환경에 따른 나트륨 함량을 비교하였다. 가정식, 외식, 학교급식에서 건더기와 국물이 1인 분량에서 차지하는 나트륨 함량은 국 및 탕류와 물김치류에서는 국물이 더 높은 비율을 차지하였으며, 찌개류와 면 및 만두류에서는 국물과 건더기가 비교적 유사한 비율을 보이고 있었다. 특히 1인 분량 내에서 국물이 차지하는 나트륨 함량은 최소 43.7%에서 최대 73.2%까지 높았으며, 이를 바탕으로 국물을 적게 섭취하는 것만으로도 상당량의 나트륨 섭취를 줄일 수 있을 것으로 기대할 수 있다. 100 g 당 나트륨 함량은 전식품에서 가정식과 외식이 학교급식보다 각각 유의하게 높았으며, 건더기에서는 외식이 가정식보다 유의하게 높았고, 국물에서는 가정식과 외식이 학교급식보다 각각 유의하게 높고 외식이 가정식보다 유의하게 높은 것으로 나타났다. 연구 결과를 바탕으로 볼 때, 동일한 종류의 음식이라 할지라도 가정식, 학교급식, 외식에 따라 나트륨 함량의 차이를 보이며 가정식과 학교급식, 가정식과 외식, 학교급식과 외식 간의 동일한 종류의 음식에서 나트륨 함량의 차이가 있어, 건더기 및 국물의 섭취 정도에 따라 실제 섭취하는 나트륨 양이 달라짐을 확인할 수 있었다. 그러므로 보다 정확한 나트륨 섭취량 추정을 위하여 음식이 제공되는 환경 및 섭취 방식에 따라 구분된 데이터베이스를 적용해야 할 것이며, 향후 관련 대규모 연구를 통해 대표성을 가진 데이터베이스의 확충이 필요할 것으로 사료된다.

• Journal of Nutrition and Health
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• 제50권6호
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• pp.655-663
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• 2017

본 연구는 전국 4권역 (수도권, 충청 강원권, 경상권, 전라권) 10개 지역의 가정, 단체급식소와 외식 음식 중 고나트륨 한식 대표 음식 16종, 총 480개 음식의 나트륨 함량을 분석하여, 나트륨 저감화 방향을 제시하고, 나트륨 함량 기준치 설정을 위한 기초자료로 활용하고자 하였다. 음식군별 100 g 당 나트륨 함량은 가정식, 단체급식, 외식 모두 김치류와 조림류에서 가장 높았고 (p < 0.001), 고나트륨 한식 대표 음식 100 g 당 나트륨 함량은 멸치볶음(1,896.6 mg)이 가장 높았고, 그 다음으로 우엉 연근조림(820.6 mg)과 양파장아찌 (809.3 mg)였다 (p < 0.001). 그러나 1인분 나트륨 함량은 가정식에서는 돼지갈비찜/찜닭(1,429.6 mg)이 가장 높았고 (p < 0.001), 단체급식에서는 비빔밥 (1,074.1 mg)과 돼지갈비찜/찜닭 (838.8 mg)이 가장 높았으며 (p < 0.001), 외식에서는 육개장 (3,273.2 mg)과 동태찌개 (2,930.2 mg)의 1인분 나트륨 함량이 가장 높았다 (p < 0.001). 외식의 국 찌개 탕류의 100 g 당 나트륨 함량 및 1인분 나트륨 함량은 가정식과 단체급식 음식보다 유의하게 높았다 (p < 0.01, p < 0.001). 이상의 결과 100 g 당 나트륨 함량은 김치류와 조림류에서 가장 높은 것으로 나타났으며, 1인분 나트륨 함량은 비빔밥, 국 찌개 탕류와 찜류 등 장류를 이용한 음식에서 높은 것으로 나타났고, 외식의 국 찌개 탕류의 나트륨 함량이 가정식과 단체급식에 비하여 높은 것으로 나타났다. 따라서 본 연구는 나트륨 저감화의 방향이 장류 및 김치류의 저염화가 우선시 되어야 하며, 외식의 국물류의 염도를 낮추고, 1인분량을 줄이려는 노력이 필요함을 시사한다. 본 연구의 전국 가정식, 단체급식, 외식 음식 고나트륨 한식 대표음식의 나트륨 함량 분석 결과는 추후 나트륨 저감화 기준치를 제시하기 위한 기초자료로 활용될 수 있을 것으로 사료된다.

• Journal of Nutrition and Health
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• 제39권6호
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• pp.549-559
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• 2006

The present study determined the estimated dietary fiber (DF) intake per capita of Korean from 1991 to 2001 and analyzed the major food sources of dietary fiber for Korean using the data on per capita consumption of each food reported in the Korean National (Health and) Nutrition Survey Reports and the recently established DF database by the Korean Nutrition Society. The estimated mean daily DF intakes of Korean during the last 11 years $(1991{\sim}2001)$ were in the range of $19.25{\sim}21.22\;g\;or\;9.97{\sim}11.99\;g/1,000\;kcal$ with a small range of fluctuations. As of 2001, average DF intake per capita of Korean was estimated as 20.92 g or 10.59 g/1,000 kcal. The average DF intake level was under the Adequate Intake for DF (12 g per 1,000 kcal) of the Dietary Reference Intakes (DRIs) for Koreans established in 2005. The level of DF intake was relatively lower in small city residents compared to the large city or rural area. The top two major food group sources of DF for Korean were vegetables and cereals, in addition, legumes, fruits, seaweeds and seasonings were included among top five food groups. The ratio of DF intake from fruits was gradually increased but the ratio from legumes was steadily decreased during the last 11 years. The mostly contributed single foods for DF intakes of Korean were Kimchi and rice regardless of year and area. Percentage of DF intake from top ten single foods was continuously decreased from 65% in 1991 to 51 % in 2001. The results of this study revealed that DF intakes of Korean as of 2001 is insufficient compared to the Adequate Intake for DF for Korean and the source of fiber in Korean diet has been more various. Therefore the beneficial health effects of DF and the increased consumption of DF from a variety of food sources should be continuously emphasized through the nutritional education.

• Journal of Preventive Medicine and Public Health
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• 제40권3호
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• pp.239-248
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• 2007

Objectives : This study was conducted to investigate the systematic error, such as seasonal change or inadequate food items, in a food frequency questionnaire administered to workers in a Nuclear Power Plant, Korea. Methods : We performed three repeat-tests with 28 subjects on May 13, July 8 and Dec 16, 1992. Our food frequency questionnaire (FFQ) comprised 84 foods organized into 7 food-groups, and was composed of the items of usual intake frequency (8 categories) and the amount per intake (3 or 4 categories) over the previous year. We compared the means of intake frequency and the frequency of the portion-size according to each season using Repeated Measures ANOVA and Pearson's chisquare test with Fisher's exact test. Results : We found the significant seasonal changes of several food items in intake frequency measurement. These items were typical seasonal foods such as mandarin orange, plum and green vegetables, while the single questions consisted of inadequate food items such as thick beef or similar soup and various kimchi products. Significant seasonal changes in portion-size were found in only two items: cooked rice-brown and fresh frozen fishes. Conclusions : The systematic errors observed could caused loss of validity in the FFQ. Consideration should be given for seasonal variation in FFQ survey and methodological concerns are needed to improve the quality for measuring usual diet pattern.

• Preventive Nutrition and Food Science
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• 제7권1호
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• pp.57-61
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• 2002

Electron spin resonance (ESR) spectroscopy was used to investigate the effect of irradiation dose and storage time on the free radical concentrations in gamma-irradiated dried seasoning powder. Seasoning powders of dried squid flavor, shrimp flavors, kimchi flavor, spicy beef soup flavor and soy sauce flavor were irradiated with doses of 0, 1, 3, 5, 7 and 9 kGy at room temperature using a Co-60 irradiator. Characteristic signals of free radicals were observed in all the irradiated samples of this experimental while these signals were not detected in non-irradiated samples. Since the free radical concentrations linearly increased with the applied doses (1~9 kGy), highly positive correlation coefficients ($R^2$ = 0.9285~0.9965) were obtained between irradiation doses and free radical concentrations during all the storage times. Free radical concentrations of the irradiated flavored seasoning powder did not change even at 16 weeks of storage at room temperature, while those of dried squid, shrimp and spicy beef soup flavors decreased until 2 weeks of storage after irradiation with 5 kGy or over, and these of soy cause flavors slowly decreased until 8 weeks of storage after irradiation with 3 kGy or over. Although the free radical concentrations decreased with storage times, the characteristic signals of the irradiated seasoning powders of dried squid, shrimp, spicy beef and soy sauce flavors were observed even after 16 weeks of storage at room temperature.