• 한국식품과학회지
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• 제41권6호
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• pp.615-621
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• 2009

SFE법을 이용하여 딸기의 휘발성 향기성분을 추출하기 위한 시료의 적정 전처리 및 SFE 작동조건을 검토하였으며, SDE법 및 SE법 등의 추출방법과 휘발성 향기성분 조성을 비교하였다. 딸기 생시료, 동결건조시료, 그리고 생시료에 celite를 30, 70%를 혼합하여 제조한 시료를 이용하여 SFE 처리후 향기성분을 분석한 결과, 생시료에 celite를 70% 첨가하는 방법이 향기성분을 추출하는데 가장 효과적인 것으로 나타났다. 온도(40, $55^{\circ}C$)와 압력(3,000, 6,000 psi)을 달리하여 SFE 처리한 결과, 3000 psi, $55^{\circ}C$ 조건에서 alcohol류와 acid류의 추출효율이 증가하였으며, $\gamma$-dodecalactone의 함량도 3배 정도 높은 수준으로 나타났다. SDE법, SFE법 및 SE법 등의 추출방법별 딸기의 휘발성 향기성분을 분석한 결과, SFE법은 SDE법에 비해 추출한 휘발성 향기성분의 종류는 적지만 추출량이 비슷하면서 열분해 및 열변성이 발생하지 않아 딸기 고유의 향기에 가까운 향기패턴을 나타내었으며, 기호적으로 우수한 acid류가 다량 함유된 향기성분을 추출하기에 적합한 것으로 나타났다.

• Applied Biological Chemistry
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• 제42권4호
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• pp.283-287
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• 1999

태광콩, 검정콩1호, 신팔달콩2호, 황금콩, 단백콩, 단엽콩을 공시재료로 하고 Bacillus subtilis var. globigii를 메주제조용 균주로 사용하여 간장을 제조하고 그 성분 특성을 조사하였다. 원료 콩의 조단백질 함량은 단백콩이 47.5%로 가장 높았으며, 유리당 함량은 검정콩1호와 단백콩이 각각 15.87%, 13.33%로 가장 높았다. Bacillus subtilis var. globigii 종균을 접종하여 만든 메주로 제조한 간장의 총질소함량은 단백콩으로 제조한 간장에서 1.18%로 가장 높았으며, 간장의 유리당과 유기산 함량은 콩 품종간에 큰 차이를 보이지 않았으며 succinic acid가 주된 유기산임을 보여주었다. 단백콩 간장의 총 유리아미노산과 glutamic acid 함량도 각각 3365 mg%와 737.44 mg%로 가장 높았다. 원료콩의 단백질 함량이 가장 높은 단백콩이 간장의 총 유리아미노산, glutamic acid 함량 및 총 아미노산에 대한 glutamic acid의 비율이 가장 높아서 간장용 콩 품종으로 가장 적합한 것으로 나타났다.

• 한국식품과학회지
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• 제42권5호
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• pp.565-570
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• 2010

본 연구에서는 coenzyme $Q_{10}$을 나노에멀젼화 하기 위해 초고압균질기를 이용하여 3가지 다른 형태의 밸브를 대상으로 평가를 진행하였으며, 선정된 밸브를 사용하여 제조된 coenzyme $Q_{10}$ 나노에멀젼의 품질 특성 및 안정성 평가를 하였다. 초고압균질기를 이용한 coenzyme $Q_{10}$ 나노에멀젼 제조 시 최적 조건은 150MPa, C 밸브, 통과 횟수 3회이었다. 제조된 나노에멀젼은 평균입자 크기가 40 nm, 제타 전위 값이 -57 mV을 나타내어 콜로이드 상태가 충분히 안정하다고 볼 수 있었다. 또한 수용액에 빠르게 분산되었으며, 이때 coenzyme $Q_{10}$ 100 mg을 함유한 증류수100 mL의 투과도 값이 90(%T)로 투명한 용액을 얻을 수 있었다. 제조한 coenzyme $Q_{10}$ 나노에멀젼은 $4^{\circ}C$ 및 $25^{\circ}C$에서 12주 동안 보존하여도 침전 또는 부유물을 발생시키지 않았고 coenzyme $Q_{10}$ 함량이 변하지 않았으며, 10일간의 동결처리 후에도 안정하였다. pH 2 용액을 제외하고는 pH(4-10) 처리와 열($95^{\circ}C$)처리 및 동결($-20^{\circ}C$)처리 시에도 안정하였다. 따라서 제조된 coenzyme $Q_{10}$ 나노에멀젼은 액체 형태 등 다양한 식품에 적용할 수 있는 가능성을 확보하였으며, 유통시에는 상온보다는 냉장 보관이 더 적합 할 것으로 판단된다.

• 한국식품영양학회지
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• 제25권2호
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• pp.308-316
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• 2012

Proper skin care promotes good metabolism and the biological activity of skin, helps maintain the skin in a healthy and beautiful state when combined with nutritional nourishment. Health functional foods are being used for the purpose of enhancing efficient skin care. Health functional foods related to skin care maintain the epidermis, dermal fibroblast layer and subcutaneous tissue that form the skin. Efficient functional foods alleviate the signs of endogenous aging that come with getting older and exogenous aging caused by sunlight. Even though the field of skin care related to health functional foods has received less attention and been the subject of less research compared to functional cosmetics which are developing widely, this area of skin care that maintains and improves the layer of dermal fibroblast through the intake of food, is expected to progress with the commercialization of products in many fields when the related technical research development is galvanized and the related patents are applied for. Research into health functional foods related to skin care in Korea started rather late in comparison to other advanced countries where patents for relevant techniques have been applied for since 1990's together with research conducted on how they might be used for practical purposes. This study used the key words 'skin care, health functional food' to search for Korean patents that have been applied for at the Korean Intellectual Property Office from 1983 to 2011 using the KIPRIS database, in order to help researchers in the related fields by organizing the patented formulas for health functional foods for skin care which have gained attention recently. According to the number of registrations in each industry field, the total number of patents was 1,120 we screened the major patents among them, the field with the largest number was food foodstuff non-alcoholic beverage and preparation or treatment(A23L) with 135, then natural plant medicine & medical supplies and cosmetics(A61K) with 112, microorganisms or enzymes(C12N) with 63, heterocyclic compounds(C07D) with 44, horticulture or cultivation of sea weeds, forestry(A01G) 16 times, listed from the highest to the lowest number of applications. It has been revealed that food, foodstuff, non-alcoholic beverage and preparation or treatment(A23L) was the industry field where the most patents for health functional foods related to skin care were registered.

• Journal of Microbiology and Biotechnology
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• 제32권12호
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• pp.1605-1614
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• 2022

The strains associated with foodborne Salmonella enterica Thompson outbreaks in Korea have not been identified. Therefore, we characterized S. Thompson strains isolated from chocolate cakes linked to foodborne outbreaks in Korea. A total of 56 strains were isolated from preserved cake products, products in the supply chain distribution, the manufacturer's apparatus, and egg white liquid products used for cream preparation. Subsequently, serological typing, pathogenic gene-targeted polymerase chain reaction (PCR), pulsed-field gel electrophoresis (PFGE), and whole-genome multi-locus sequence typing (wgMLST) were performed to characterize these isolates. The antigen formula of all isolates was 7:k:1,5, namely Salmonella enterica subsp. enterica Serovar Thompson. All 56 isolates harbored invA, his, hin, and stn, and were negative for sefA and spvC based on gene-targeted PCR analyses. Based on PFGE results, these isolates were classified into one group based on the same SP6X01.011 pattern with 100% similarity. We selected 19 strains based on the region and sample type, which were subjected to wgMLST. Although the examined strains showed 100% similarity, they were classified into seven clusters based on allelic differences. According to our findings, the cause of these outbreaks was chocolate cake manufactured with egg white liquid contaminated with the same Salmonella Thompson. Additionally, comparative analysis of wgMLST on domestic isolates of S. Thompson from the three outbreaks showed genetic similarities of over 99.6%. Based on the results, the PFGE and wgMLST combination can provide highly resolved phylogeny and reliable evidence during Salmonella outbreak investigations.

• 한국식품조리과학회지
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• 제22권1호
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• pp.56-61
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• 2006

This study investigated the quality characteristics of jelly using sericultural products which were manufactured with various addition levels(0.5, 1, 1.5, 2%) of mulberry leaf powder. To establish the additional amount of mulberry leaf powder, physicochemical, sensory, and texture results were analyzed. The moisture and crude protein content of Pongnipjelly were higher than that of the control(p<0.05). The mineral content was increased with increasing addition levels of mulberry leaf powder. The color of Pongnipjelly darkened with increasing addition levels of mulberry leaf powder. As for the texture analyser, the hardness, gumminess, and chewiness increased with increasing addition of mulberry leaf powder(p<0.05), whereas the springiness decreased. Therefore, the optimal added amount of powder for the manufacture of Pongnipjelly was determined to be 1.5% of the total weight.

• 한국축산식품학회지
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• 제34권1호
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• pp.127-132
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• 2014

Optimization of carnosine and anserine extraction from chicken breast was performed using response surface methodology (RSM) to obtain the maximized physiological activities for anti-glycation and anti-oxidation. The optimum extraction conditions were water extraction for 1.6 h in the case of the 20-wk laying hen muscle and water extraction for 2.12 h in the case of 90-wk laying hen muscle. Higher carnosine and anserine contents were measured in the 20-wk laying hen muscle, along with higher physiological activities, which increased in direct proportion with the dipeptide contents. The extracts prepared from the 20-wk laying hen under optimum conditions showed 57% inhibition of advanced glycated end-product formation, 64% inhibition of lipid peroxidation, and 61% of DPPH radical scavenging effects. On the other hand, 52% inhibition of AGE formation, 62% inhibition of lipid peroxidation, and 53% of DPPH radical scavenging effect were demonstrated within the 90-wk laying hen. In addition, the ratio of carnosine was a key indicator for the physiological activities of the extracts.

• 한국축산식품학회지
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• 제33권5호
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• pp.572-580
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• 2013

This study was carried out to evaluate the effect on properties of restructured jerky by the addition volume of additives. The treatments were divided into glutinous rice flour, potato starch, soybean and acorn powder. Moisture content, water activity ($a_w$), thiobarbituric acid reactive substances (TBARS), color, yield and sensory evaluation were performed. The moisture contents of four types of restructured jerkies were in the range of 8.92-12.47%, and were lower than that of the control (17.92%). Water activity tended to decreased with increasing addition of all treatments. The restructured jerkies containing glutinous rice flour, potato starch, soybean and acorn powder had lower TBARS than the control. The drying yield tended to increase with increasing amount of additives. In the sensory evaluation results, the highest overall acceptability was found in jerky containing glutinous rice flour, potato starch, and acorn powder when the addition was 9%, while that for soybean powder was determined to be 5%. These results suggest that 9% additions of glutinous rice flour, potato starch or acorn, or 5% soybean powder are optimal addition volumes for the preparation of restructured jerkies.

• 한국미생물·생명공학회지
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• 제19권2호
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• pp.158-162
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• 1991

Endoinulinase was purified from a commercial inulin preparation produced by Aspergillus ficuum using ion exchange chromatography on CM-Sephadex C-50 and DEAE-Sepharose 6B, HPLC gel filtration on a Protein Pak 125 Colum and HPLC ion exchange chromatography on a TSK DEAE-5pw Column. The endoinulinase had a molecular weight of 72,000${\pm}$1,000 and was glycoprotein with 23 to 25% w/w sugar content. The enzyme was much more active on inulin with random cleavage mode than on sucrose and on palatinose: The ration of activity on inulin and sucrose (I/S ratio) was 10~14.

• 한국식품저장유통학회지
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• 제17권2호
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• pp.223-229
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• 2010

20 L 부피의 발효용기에서 어간장을 발효하면서 용기의 내부 온도 변화와 염도, pH, 산도, 총질소, 유리아미노산, 미생물 변화를 분석하였고 4개월 발효후의 어간장의 관능적 품질을 평가하였다. 발효용기로는 새 옹기와 재사용한 옹기, 스테인레스, 플라스틱 용기를 사용하였고 발효숙성은 외기조건에서 이루어졌다. 옹기가 가지는 통기성 및 보온성으로 인하여 발효 중 관여하는 미생물의 생육환경을 적절하게 조성하여 여러 가지 품질에 긍정적인 영향을 미치는 것을 확인할 수 있었다. 특히 옹기는 새 것보다는 재사용한 옹기에서 제품의 발효에 좋은 영향을 미쳐서, 높은 총질소, 유리아미노산함량, 향, 맛 전체적인 수용도의 관능적 품질을 보여주었다.