• Title/Summary/Keyword: foliar-spraying

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Enhancement of Biological Control of Botrytis cinerea on Cucumber by Foliar Sprays and Bed Potting Mixes of Trichoderma harzianum YC459 and Its Application on Tomato in the Greenhouse

  • Lee Sun-Kug;Sohn Hwang-Bae;Kim Geun-Gon;Chung Young-Ryun
    • The Plant Pathology Journal
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    • v.22 no.3
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    • pp.283-288
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    • 2006
  • Trichoderma harzianum YC459 (Th 459), isolated from sawdust compost, was effective in controlling cucumber and tomato gray mold caused by Botrytis cinerea under controlled and plastic film tunnel conditions. A water suspension of the wettable powder formulation of Th 459 significantly $(P\leq0.05)$ reduced the severity of cucumber gray mold by foliar spraying at all tested concentrations from $10^5\;to\;10^8$ colony forming unit (cfu)/ml in repeated experiments. The control efficacy was maintained at least seven days with the average control value of 70% in cucumber pot tests. Mixing one to eight grams of the granular formulation ($10^8cfu/g$ dry weight) of Th 459 into one liter nursery potting mix at seeding also significantly $(P\leq0.05)$ reduced the severity of cucumber gray mold by suppression of lesion formation three weeks after treatment. Application of mixing granular formulation at seeding in combination with foliar spraying during cultivation provided a more significant reduction $(P\leq0.05)$ of cucumber gray mold than granule mixing or leaf spray alone. The foliar spraying of the formulated wettable powder of Th 459 significantly $(P\leq0.05)$ reduced the infection of tomato fruits by B. cinerea as effective as the chemical fungicide, dichlofluanid, in three plastic film tunnel experiment trials. It is suggested that effective control of gray mold of cucumber and tomato can be provided by both treatment of Th 459 into potting mix and foliar spray through induction of systemic resistance and direct inhibition of the pathogen.

Effects of Foliar Spray of Calcium Hydroxide on Shoot Growth and Fruit Quality in 'Daewol' Peach (Prunus persica Batsch) (수산화칼슘 엽면살포가 '대월' 복숭아의 신초 생육 및 과실 품질에 미치는 영향)

  • Park, Ji-Young;Son, In-Chang;Kim, Dae-Il
    • Korean Journal of Environmental Agriculture
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    • v.29 no.2
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    • pp.102-108
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    • 2010
  • The effects of foliar spray of calcium hydroxide ($Ca(OH)_2$) during fruit growth period was investigated by changes of the shoot growth and fruit quality in 'Daewol' peach (Prunus persica). Since foliar spray of $Ca(OH)_2$ 1, 2, 5, and 10 $g{\cdot}L^{-1}$ at 7 day-intervals after fruit thinning, shoot growth was remarkably decreased compared with control group. An average SPAD value of fifth leaf from proximal part of the shoot was higher as 42.1 specific color difference sensor value (SCDSV) of $Ca(OH)_2$ foliar spraying treatments than 40.9 SCDSV of control group. Photosynthesis rates were also significantly increased by treating $Ca(OH)_2$ of higher concentration. Among fruit characteristics affecting quality, fruit weight was increased depending on concentration of $Ca(OH)_2$ treatment. The soluble solids content was lowest in control group (8.78 $^{\circ}Brix$) compare with higher concentrations of $Ca(OH)_2$ foliar spraying treatment in each 9.17, 9.22, 9.71, 10.58 $^{\circ}Brix$. The acidity and anthocyanin contents were no significant differences among treatment, but firmness of pericarp and flesh of fruits was significantly increased by $Ca(OH)_2$ foliar spray treatment. As a results of morphological observation of leaf, thickness of palisade parenchyma was thinner in control group (63.5 ${\mu}m$) than those of each 86.5, 87.5, 93.6, 107.4 ${\mu}m$ in $Ca(OH)_2$ foliar spraying treatment. Higher $Ca(OH)_2$ foliar spray also increased the thickness of cell wall of epidermis and hypodermis in 'Daewol' fruit.

Potential for Augmentation of Fruit Quality by Foliar Application of Bacilli Spores on Apple Tree

  • Ryu, Choong-Min;Shin, Jung-Nam;Qi, Wang;Ruhong, Mei;Kim, Eui-Joong;Pan, Jae-Gu
    • The Plant Pathology Journal
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    • v.27 no.2
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    • pp.164-169
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    • 2011
  • Previous studies have addressed the management of phyllosphere pathogens by leaf and root-associated microbes. The present study evaluated the effect of the foliar application of three strains of Bacillus spp. on plant growth and fruit quality. The application of a bacilli spore preparation significantly improved leaf growth parameters such as leaf thickness and photosynthesis capacity, indicating that bacilli treatment directly promoted leaf growth. In addition, foliar treatment resulted in an improvement in the key indicators of fruit quality including water, glucose, and sucrose contents. The present results suggest that foliar spraying of beneficial bacilli is a potential treatment of wide application for the improvement of apple quality. Foliar application of bacilli preparation as effective plant growth-promoting rhizobacteria broadens the spectrum of their availability for orchard application.

Factors Influencing the Foliar Uptake of Dimethomorph into Cucumber Induced by Fatty Alcohol Ethoxylate (Fatty Alcohol Ethoxylate에 의해 유도되는 Dimethomorph의 오이 엽면 침투성 영향 인자)

  • Yu, Ju-Hyun
    • The Korean Journal of Pesticide Science
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    • v.12 no.2
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    • pp.118-126
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    • 2008
  • In order to find the best compositions of the dimethomorph formulation containing fatty alcohol ethoxylate surfactants as an activator adjuvant and recommend the good management way of the formulations to allow an intended uptake rate, factors affecting foliar uptake of active ingredients into cucumber were investigated and compared each other. Among tested, the most important factors were air temperature in which plants were located after spraying of aqueous formulations containing activator adjuvant, and deposit of wax on the leaf surface of cucumber. Dimethomorph uptake was found to be increased by sunlight. When aqueous formulations had long-polyethoxylated fatty alcohol surfactants, the relative humidity of the room in which the plants were located after spraying with the formulations did not severely influence the foliar uptake. Maintaining a mild temperature and avoiding too strong sunlight after spraying with dimethomorph formulations were found to be resonable management strategies.

Effects of Foliar Application of CaCl2 on Ca Content and Occurrence and Alleviation of Blossom-end Rot of Paprika During High Temperature Season (고온기 CaCl2 엽면 살포가 파프리카 칼슘함량, 배꼽썩음과 발생과 경감에 미치는 영향)

  • Oh, Jeong Sim;Lee, Yong-Beom;Bae, Jong Hyang;Na, Jong Kuk;Choi, Ki Young
    • Journal of Bio-Environment Control
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    • v.30 no.4
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    • pp.263-270
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    • 2021
  • This experiment was carried out to determine the effect of CaCl2 foliar spraying on the inhibition of blossom-end rot (BER) in hot summer paprika (Capsicum annum L. 'Special') cultivation. The effect of CaCl2 application was examined by the foliar application based on different fruit size, frequency, and spraying time. Also, it was investigated the occurrence and alleviation effect of BER symptom. Foliar application of CaCl2 (Ca 0.4%) was conducted by treating a fruit and leaf at 4 to 9 nodes above the crown flowers of each plant with 350 mL per week from June 3 to July 1. When the CaCl2 was sprayed at 7-day intervals for 4 weeks, the Ca content was the lowest in the fruit harvested with BER symptom in 11 to 20 mm of fruit width (FW). Four different regions in both BER symptom and normal fruits showed significant differences of Ca content, the highest was in pedicel, followed by stem-end, middle, and blossom-end. The Ca content increased sharply in normal paprika with 31-40 mm FW, in which Ca content was 78% higher than that of BER. Ca content in the middle and blossom-end of paprika over 21 mm in FW ranged 19.8% to 28.8% in normal fruits and 15.7% to 18.5% in BER, respectively. The incidence of BER increased rapidly by more than 60% in fruits with 31-40 mm FW. While there was no difference in fruit weight among the FW treatment, marketable yield rate was highest in the 21-30 mm FW, and the sugar content was high in the 11-30mm FW. When CaCl2 was applied three times for 7 days to a paprika having a 21- 30 mm FW, the cell wall-bound (CWB) Ca content was the highest and the rate of BER was lowest with 6.3%. After 10 days of CaCl2 foliar spray treatment, the CWB Ca content of paprika increased by 2.9 to 3.5 times compared to the control in all treatments. At 7 days after the CaCl2 foliar spraying once a day at varying spraying time, the leaf burn observed from 9:30 a.m. to 17:00 p.m. and the proline content increased as the spraying time was delayed. Therefore, the CaCl2 foliar spraying method for reducing of BER occurrence during paprika summer cultivation seems to be appropriate to spray 2-3 times at intervals of 3 days and before 8 a.m. at the time when the FW is 21-30 mm.

Effects of β-Glucans from Aureobasidium pullulans on Cucumber Mosaic Virus Infection in Chili Pepper

  • Yoon, Ju-Yeon;Gangireddygari, V.S.R.;Cho, In-Sook;Chung, Bong-Nam;Yoon, Byung-Dae;Choi, Seung-Kook
    • Research in Plant Disease
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    • v.27 no.1
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    • pp.17-23
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    • 2021
  • Cucumber mosaic virus (CMV), the most prevalent virus in chili pepper (Capsicum annuum L.), negatively affects chili pepper production in South Korea. In this study, foliar spraying with β-glucans obtained from the mycelial walls of the yeast-like fungus Aureobasidium pullulans inhibited CMV infection of chili pepper if applied before virus inoculation. At three concentrations, β-glucans from A. pullulans significantly ameliorated CMV symptoms in treated chili pepper; the effect was greater in plants treated with 0.01% β-glucans than 0.005% or 0.001% β-glucans. Double antibody sandwich enzyme-linked immunosorbent assay showed that these β-glucans treatments resulted in 1.7- to 10-fold reductions in CMV accumulation in the treated chili pepper. The glucans did not act directly on the virus and did not interfere with virus disassembly or replication. Foliar spraying with 0.01% β-glucans from A. pullulans at 24 hr intervals for 3 days significantly increased plant height, the total number of fruit, and the fresh weight of chili pepper fruit. However, the stem diameter of chili pepper treated with β-glucans did not increase significantly. These results indicate that foliar spraying with β-glucans from A. pullulans acts an antiviral agent against CMV infection and stimulates chili pepper growth.

Evaluation of Foliar Uptake of Eight Fungicides Using a New Measuring Tool, Congo Red Method

  • Yu, Ju-Hyun;Lim, He-Kyoung;Cho, Kwang-Yun;Kim, Jeong-Han
    • Journal of Applied Biological Chemistry
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    • v.44 no.1
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    • pp.27-31
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    • 2001
  • Using a new evaluation method, Congo red method, the foliar uptakes of eight fungicides were measured. Among the fungicides tested, fenarimol, myclobutanil, edifenphos, isoprothiolane, and tricyclazole were found to be penetrable (11.0~42.8%), and were continuously absorbed into the leaf regardless of their formulation types. Procymidone, dimethomorpb, and ethaboxan were seldomly absorbed into plant leaf. The uptakes of dimethomorph and ethaboxam were facilitated (18.1~22.7%) in the presence of Koremul-OE-20, as an activator, showing plateau phase at 12h after application. Foliar uptake of fenarimol and myclobutanil into cucumber plant by spray application was larger than that by dropping application, while observed opposite in the cases of dimethomorph and ethaboxam containing activator. Congo red method in conjunction with spraying application used in the present study produced the clear results on foliar uptake of fungicides and therefore proved to be better than the conventional method in which the dropping application of pesticide has been used.

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Establishment of Foliar Application Assays for Developing Natural Herbicides (천연물 제초제 개발을 위한 전식물체 수준의 경엽처리 검정법 확립)

  • Kim, Jae-Deog;Jang, Hyun-Woo;Seo, Bo-Ram;Hwang, Hyun-Jin;Choi, Jung-Sup;Kim, Jin-Seog
    • Korean Journal of Weed Science
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    • v.30 no.2
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    • pp.153-163
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    • 2010
  • This study was carried out to establish an improved bioassay system, whole-plant bioassay which is more effective in developing natural herbicides for foliar treatment such as herbicidal essential oils. Two bioassay systems using four weed species (Echinochloa crus-galli, Digitaria sanguinalis, Aeschynomene indica, and Abutilon theophrasti), spraying method and spotting method, were established. Spraying method is applicable if the amount of test compounds is enough, while spotting method is useful for the small amount of test compounds. The initial application rate was desirable at $2,500{\sim}5,000\;{\mu}g\;mL^{-1}$. Herbicidal activities were higher in the NOP treatment when compared to the Tween 20 treatment. To efficiently evaluate volatile compounds such as essential oils, if the compound-treated pots were incubated in dew chamber for about 10hrs, better results were obtained in the degree and stability of herbicidal responses. When the efficiency of bioassay systems established in this study was compared, the spraying method was minimized four times to the conventional method that has beed used for screening of synthetic compounds in KRICT. On the other hand, in the spotting method, screening for development of a natural herbicides was possible even in level of 1/100 test volume and 1/200 amounts of test compound compared to the spraying method.

Effects of PP333 Spraying on Growth of Soybean (PP 333 처리가 대두 생육에 미치는 영향)

  • 정병관
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.32 no.2
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    • pp.181-187
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    • 1987
  • The objectives of the study were to reduce lodging during the stormy rain period of soybean growing season in Korea, and to find out the most efficient foliar spraying of pp 333 solution in order to increase tolerance to lodging of soybean plants. The concentration of the pp 333 solution were three, five and eight percent and the time of spraying were 35th day after seeding and at flowering time. The most efficient foliar spraying practice was to spray five percent solution of pp 333 on the 35th day after seeding, resulting in reduced stem length damage of soybeans alone with shortened plant height and stabilized yield of soybean grains.

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Effects of Fertilizer and Regulator Application on Shortening of Plant Height and Yield of Sesame (시비방법과 생장조정제 처리가 참깨 초장단축 및 수량에 미치는 영향)

  • 정병관
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.36 no.3
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    • pp.259-265
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    • 1991
  • This experiment was carried out to find of shortening degree of plant height and seed yield of sesame by different treatment of fertilizer and regulator application. Treatment of higher yield was compost, foliar spraying of Tachigaren chemicals and pinching + 1. 5 times as much as control application of 3 elements which was nitrogen, phosphate, potassium. Especially, Tachigaren chemicals plot was showed increasing lodging resistance because of shortening stem of 6cm compare with control plot and more 8% than control plot in seed yield. Expectant treatment of seed yield was application of compost 2,000Kg per l0a. Foliar spraying of Tachigaren chemicals at 6 leaves full-open of main stem was expected higher yield as well as shortening stem.

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