• 한국자원식물학회지
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• 제26권3호
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• pp.383-388
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• 2013

The research concerned of the regeneration of plants from embryos obtained from anther cultures of ginseng (Panax ginseng C. A. Meyer). The aim was to determine the influence of the regeneration medium on the efficiency of the regeneration process. We conducted to determine the optimum conditions such as cold pretreatment, plant growth regulators and carbon sources on anther culture of P. ginseng. Highest callus formation rate was obtained when flower buds pretreated at $4^{\circ}C$ for 1 day. Among the treated growth regulators with various degrees of concentration in Murashige and Skoog's (MS) medium, 4.53 ${\mu}M$ of 2.4-dichlorophenoxyacetic acid and 4.44 ${\mu}M$ of 6-benzylaminopurine gives the most responsive callus with the frequency of 73.89% and 129.53 g of fresh weight. When we used 3-9% of sucrose and maltose among the different kinds and various concentrations of carbohydrates, callus was formed highest 67.29% in the medium with 3% of sucrose. Shoots induced from callus supplemented with 28.9 ${\mu}M$ of gibberellic acid and rooted in Gamborg's B5 medium supplemented with 14.7 ${\mu}M$ of indole-3-butyric acid.

• 한국약용작물학회지
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• 제17권4호
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• pp.251-256
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• 2009

The parts of leaves, flowers and stems in ginseng were obtained for analyzing the component of saponin on 15th April, 25th April, 5th May, 25th May, which were considered as ginseng foliation stage. The total saponin content of the leaves were 97.29, 66.42, 67.61, 36.24 mg/g, respectively, in which the content of Re, $Rb_1$ and Rd were more than 2/3 amount of total saponin. Especially, the saponin content of leaves decreased according to the sequential collection days, in which the similar results were observed from the flowers and stems of ginseng. The total saponin content of the flowers and stems were 141.09,143.84,139.25,133.47 and 13.32, 9.85, 8.00, 4.65 mg/g, respectively. Among them, the content of Re, Rd and $Rb_2$ in flowers were more than 2/3 while the content of Re, $Rg_1$ and Rd in stems showed more than 9/10 amount of total saponin. The total saponin content of individual leaf were 19.46, 28.56, 58.82 and 169.24 mg/plant, 2.53, 2.76, 5.20 and 12.32 mg/plant in stems, and 14.11, 30.21, 37.60 and 73.41 mg/plant in flowers. Therefore, the total saponin content of aboveground parts in ginseng were leaves > flowers > stems.

• The Plant Pathology Journal
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• 제22권1호
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• pp.51-62
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• 2006

Three strains of Cucumber mosaic virus (CMV) have been found to cause a lethal disease, referred to as fern leaf syndromes and mild mosaic symptoms in tomato (Lycopersicon esculentum Mill.) crops grown in Kuwait. CMV strains were detected and identified based on host range, symptomatology, serology, electron microscopy, and ribonucleic acid (RNA) electrophoresis in polyacrylamide gels. A high degree of viral genomic heterogeneity was detected among CMV strains isolated in Kuwait, with no apparent correlation to symptomatology in tomato host plants. Two different virus satellites of 'CMV associated RNA 5', designated CARNA 5, were detected in two virus strains that caused both lethal disease and mild symptoms, designated CMV-D1 and CMV-S1 respectively. CARNA5 was not detected in the third CMV strain that caused fern leaf syndromes designated CMV-F. All the three isolated strains were serologically indistinguishable from each other and may belong to one serotype according to Ouchterlony gel diffusion tests. These strains transmitted via aphids (Myzus persicae Sulz) in a non-persistent manner. Physical properties of the virus strains were very similar where thermal inactivation test showed that virus withstood heating for 10 min at $70^{/circ}$, dilution end point was $10^{-4}$, and the longevity in vitro at room temperature was less than 5 days for all virus strains. CMV-D1 and CMV-F were the most devastating diseases spreading in both greenhouse and field-grown tomato where aborted flower buds failed on fruit setting due to the viral infection. This is the first report to isolate three different strains of CMV in Kuwait.

• 한국약용작물학회지
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• 제15권3호
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• pp.189-193
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• 2007

사철쑥 (Artemisia capillaris Thunb.)의 수확시기 및 부위별 생육특성, scoparone과 capillarisin함량을 조사하여 약리 성분 함량이 높은 수확시기와 부위를 알아본 결과는 다음과 같다. 사철쑥은 7월 30일경 꽃봉오리가 형성되었으며 8월 30일 만개하여 9월에 종자가 성숙되었다. 생육은 8월 30일에 가장 양호하여 생체중과 건물중이 높았다. 특히 이 시기에 엽은 관찰되지 않았고 생체중은 243.7g으로 높았는데 종실의 무게는 109.6g, 줄기의 무게는 134.Is이었다. Scoparone 함량은 종실에 많이 함유되어 있었는데 8월 30일 종실에서 6.50mg/g DW로 가장 높았다. 또한 capillarisin은 줄기에는 존재하지 않았으며 엽과 종실에서 나타났는데 7월 30일 종실에 1.65mg/g DW로 가장 많이 함유되어 있었다.

• 생물환경조절학회지
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• 제22권3호
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• pp.214-219
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• 2013

본 실험은 임파첸스 분화식물의 생장과 개화에 LED 광질과 효과 적정 처리 시간을 구명하기 위해 수행되었다. 임파첸스의 초장은 2.50cm로 청색광에 의해 촉진되었으며 대조구에 비해 0.6cm의 차이를 보였다. 절간수, 뿌리길이, 분지수, 직경은 보광시간이나 광질에 영향을 받지 않았다. 엽면적은 412.81$cm^2$의 대조구에 비해 광질처리 시 626.64~784.53$cm^2$로 증가하였다. 광질처리에 의해 화아수와 개화수는 감소하는 경향을 보였다. 그러나 개화소요일수는 광질처리에 의해 단축되어 개화를 촉진하였으며 적색광에서 좋았다. 엽록소 함량은 광질처리에 의해 크게 영향을 받지 않았으나, 안토시아닌 함량은 전반적으로 일몰 후 4시간의 청색광에서 증가하였다. 광질처리는 청색광 처리에서 생체중과 건물중이 향상되었다.

• Journal of Crop Science and Biotechnology
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• 제11권1호
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• pp.23-30
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• 2008

The relationship between three phenological parameters and somatic embryogenesis was investigated during a two-year period. Staminodes and petals from six hybrids and two clones as controls were sowed on three distinct primary callus growth media. Flowering level, fructification level, and leaf thrusts rhythm as phenological parameters were measured simultaneously during the weekly harvest of flower buds. Mean and coefficient of variation of the measured parameters highlighted stable phenological phases. The relationship between phenological parameters and somatic embryogenesis was investigated first by comparing the variation of somatic embryogenesis and that of the phenological parameters, and second by using Pearson's linear correlation. Except for the fructification level in both control clones the first year, the other parameters recorded stable phenological phases, regardless of the genotype and year. Favorable and unfavorable phases for the somatic embryogenesis were identified. In hybrids, favorable phases included February, August, September, and October. In both control clones, time interval propitious to embryogenesis stretched from February to December. The significance of the coefficient of correlation seemed to establish a relationship between somatic embryogenesis and phenology. However, a causal link could not be established. Leaf thrusts rhythm was revealed to be the phenological parameter most linked to somatic embryogenesis. Attempts to optimize embryogenesis during unfavorable phases, showed that a correction of 2.4 D/TDZ concentration is not the solution.

• 한국식품위생안전성학회지
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• 제7권2호
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• pp.83-89
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• 1992

정향의 mathanol 추출물로 HCI-ethanol 위손상 실험을 하여 그 방어 효과를 확인하고 이를 계통 분획한 후 각 분획에 대한 HCI-ethanol 위손상실험, 기초위액 분비실험, indomethancin 위손상실험, acetic acid 궤양 실험을 하여 추출물에 대한 급성독성과 급.만성위염 및 위손상에 대한 실험한 바 다음과 같은 결과를 얻었다. (1) $LD_{50}$은 경구투여시 3000 mg/Kg 이사이었다. (2)HCL.ethanol 위손상실험에서 EtOAc fr. 165mg/Kg과 BuOH fr. 215mg/Kg에서 손상의 크기를 유의성있게 감소시켰다. (3) Acetic Acid 궤양 실험에서 위궤양의크기가 BuOH 분획 215mg/Kg으로 유의성 있게 감소되었다. 이상의 결과로서 생각은 급만성 위염 및 위손상에 대한 세포보호 작용이 있으며 그 작용기전은 위액분비의 억제작용에 인한 것이며 그 유효성분은 대부분 butanol 분획에 존재하는 것으로 생각된다.

• 원예과학기술지
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• 제19권3호
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• pp.348-351
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• 2001

'새나라' 품종은 농촌진흥청 원예연구소에서 1997년에 육성한 중생종으로 착색과 품질이 우수하고 풍산성이다. 이 품종은 1981년도에 'Spur EarliBlaze'에 'Spur Golden Delicious'를 교배하여 육성한 것으로 1992년도에 1차 선발되어 '원교 가-08호'로 명명되었고 1993년부터 1997년까지 5년 동안 7개 지역에서 지역적응성시험을 거쳐 1997년에 '새나라'로 최종 선발 및 명명되었다. 수세는 중정도이고 수자는 개장성이며 스퍼 타입이다. 개화기는 'Spur Golden Delicious'보다 하루 빠르고 'Spur EarliBlaze'보다 3일 늦다. 수원지역에서의 숙기는 만개기로부터 140일 후인 9월 10일경이고 과형은 원추형이며 과피색은 선홍색이다. 과중은 300g 전후이고 당도는 $14.6^{\circ}Brix $이며 점무늬낙엽병에 약하다.

• 한국약용작물학회지
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• 제7권4호
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• pp.269-274
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• 1999

홍화(紅花)의 안전다수확(安全多收穫) 재배기술(栽培技術) 확립(確立) 일환(一環)으로 무처리(無處理), 파종시(播種時), 개화시(開花時), 개화기(開花期), 개화후(開花後) 10일부터 수확기(收穫期)까지 비가림재배의 효과를 구명코자 시험한 결과를 요약하면 다음과 같다. 홍화(紅花)는 일찍 비가림 재배에 의하여 출아기(出芽期)와 개화기(開花期)가 빨랐으며 지상부 생육이 양호하였다. 10a당 종실(種實)은 파종부터 수확기까지 일찍 비가림한 것이 387kg으로 노지재배 198kg에 비해 95%증수 되어 비가림 적기로 판단되었다. 홍화 종실의 색도는 파종부터 수확기까지 비가림 한 것이 명도가 가장 높고 적색도와 총색도가 가장 낮아 거의 횐색 을 나타내었다. 비가림 시기에 따른 경제성(經濟性)은 파종부터 수확기까지 비가림이 가장 높았는데 노지재배 10a당 소득 3,675천원에 비해 파종부터 수확기까지 비가림은 6,705천원으로 82%정도 소득이 향상되었다.

• The Plant Pathology Journal
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• 제20권1호
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• pp.46-51
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• 2004

Host resistance is usually parasite-specific and is restricted to a particular pathogen races, and commonly is expressed against specific pathogen genotypes. In contrast, resistance shown by an entire plant species to a species of pathogen is known as non-host resistance. Therefore, non-host resistance is the more common and broad form of disease resistance exhibited by plants. As a first step to understand the mechanism of non-host plant defense, expressed sequence tags (EST) were generated from a hot pepper leaf cDNA library constructed from combined leaves collected at different time points after inoculation with non-host soybean pustule pathogen (Xanthomonas axonopodis pv. Glycines; Xag). To increase gene diversity, ESTs were also generated from cDNA libraries constructed from anthers and flower buds. Among a total of 10,061 ESTs, 8,525 were of sufficient quality to analyze further. Clustering analysis revealed that 55 ％ of all ESTs (4685) occurred only once. BLASTX analysis revealed that 74％ of the ESTs had significant sequence similarity to known proteins present in the NCBI nr database. In addition, 1,265 ESTs were tentatively identified as being full-length cDNAs. Functional classification of the ESTs derived from pathogen-infected pepper leaves revealed that about 25％ were disease- or defense-related genes. Furthermore, 323 (7％) ESTs were tentatively identified as being unique to hot pepper. This study represents the first analysis of sequence data from the hot pepper plant species. Although we focused on genes related to the plant defense response, our data will be useful for future comparative studies.