Purpose : This study quantitatively evaluated the apoptosis In human peripheral blood lymphocytes using flow cytometry, and investigated the possibility of using this method, with a small amount of blood, and the time and dose dependence of radiation-induced apoptosls. Materials and Methods : Peripheral blood lymphocyes were isolated from the heparinized venous blood of 11 healthy volunteers, 8 men and 3 women, with each 10 ml of blood being divided Into IS samples. The blood lymphocytes were Irradiated using a linear accelerator at a dose rate of 2.4 Gy/min, to deliver doses of 0.5, 1, 2 and S Gy. The control samples, and Irradiated cells, were maintained in culture medium for 24, 48 and 72 hours fellowing the Irradiation. The number of apoptotic cells after the in vitro X-irradiation was measured by flow cytometry after Incubation periods of 24, 48 and 72 hours. We also observed the apoptotic cells using a DNA fragmentation assay and electron microscopy. Results : The rate oi spontaneous apoptosis increased in relation to the time interval following irradiation (1.761 ${\pm}$0.161, 3.563${\pm}$0.554, 11.098${\pm}$2.849, at 24, 48, and 72 hours). The apoptotli cells also increased In the samples irradiated with 0.5, 1, 2 and 5 Gy, In a radiation dose and time interval after Irradiation manner, with the apoptosls being too great at 72 hours after Irradiation. The dose-response curves were characterized by an Initial steep Increase In the number of apoptotic cells for Irradiation doses below 2 Gy, with a flattening of the curves as the dose approached towards 5 Gy. Conclusion :The flow cytometric assay technique yielded adequate data, and required less than 1 mL of blood. The time and dose dependence of the radiation-induced apoptosis, was also shown. It is suggested that the adequate time Interval required for the evaluation of apoptosis would be 24 to 48 hours after blood sampling.
Journal of Korean Society of Environmental Engineers
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v.28
no.3
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pp.308-315
/
2006
Since sewage characteristics are the most important factors that can affect the biological reactions in wastewater treatment plants, a detailed understanding on the characteristics and on-line measurement techniques of the influent sewage would play an important role in determining the appropriate control strategies. In this study, samples were taken at two hour intervals during 51 days from $1^{st}$ October to $21^{st}$ November 2005 from the influent gate of sewage treatment plant. Then the characteristics of sewage were investigated. It was found that the daily values of flow rate and concentrations of sewage components showed a defined profile. The highest and lowest peak values were observed during $11:00{\sim}13:00$ hours and $05:00{\sim}07:00$ hours, respectively. Also, it was shown that the concentrations of sewage components were strongly correlated with the absorbance measured at 300 nm of UV. Therefore, the objective of the paper is to develop on-line estimation technique of the concentration of each component in the sewage using accumulated profiles of sewage, absorbance, and flow rate which can be measured in real time. As a first step, regression analysis was performed using the absorbance and component concentration data. Then a neural network trained with the input of influent flow rate, absorbance, and inflow duration was used. Both methods showed remarkable accuracy in predicting the resulting concentrations of the individual components of the sewage. In case of using the neural network, the predicted value md of the measurement were 19.3 and 14.4 for TSS, 26.7 and 25.1 for TCOD, 5.4 and 4.1 for TN, and for TP, 0.45 to 0.39, respectively.
Journal of Korean Tunnelling and Underground Space Association
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v.10
no.4
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pp.383-395
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2008
The grouting is one of the improved techniques which is aim to decrease the permeability and to strengthen the soft ground. But The grouting method has many problems about a suitability of grouting procedure and an effectiveness of grouting after grouting work because of a technical characteristic operated inside the soil. The grouting $p{\sim}q{\sim}t$ chart of a typical index about grouting rate and time alteration of grouting pressure is one method to estimate the suitability of grouting factor with monitoring during grouting procedure. This study is automatic grouting system (AGS) which can control the testing and grouting procedures. It can make the detailed $p{\sim}q{\sim}t$ chart and analyze the grouting characters of the ground by comparing the detailed pattern of $p{\sim}q{\sim}t$ chart with standard pattern. If using the $p{\sim}q{\sim}t$ chart derived from AGS in the grouting work, it is an objective standard estimating the suitability of grouting factor with grouting materials, grouting method, grouting rate and grouting pressure, as results it expects successfully to improve reliability of the grouting work.
Android platform is a powerful operating system developed on Linux 2.6 Kernel, and provides many features such as comprehensive libraries, a multimedia environment, and powerful interface for phone applications. Since Android is an open operating system, which can be installed in any vendors's equipments. Current smartphones as well as netbooks, navigations, car PCs, tablet PCs, Industrial PCs are used in various fields. It is difficult a lot that to mount to other devices on the Android platform or new devices. In this Paper, The process that data that occurred from a hardware was passed to the highest application and Android platform system for managing hardware devices were analyzed. Building Android & driver compilation environment, How to support the protocol for the use of WiFi in the kernel, How to Mount a WiFi device in the kernel, Device driver registration for the Android platform, WiFi Management Service Daemon (wpa_supplicant) and IP allocation services daemon (dhcpcd) registration, How to create a socket for communication between the daemon (wpa_supplicant) and HAL have been presented. In the experiment using the proposed method, WiFi devices were mounted on the Android platform in the X-86 & ARM family. Understanding the whole process of control flow in Android hierarchy is very important to porting a new device on it. The process included in this paper can help technicians who might encounter the obstacles in their porting works.
In the recent priority system, high-priority packet will be served first and low-priority packet will be served when there isn\`t any high-priority packet in the system. By the way, even high-priority packet can be blocked by HOL (Head of Line) contention in the input queueing System. Therefore, the whole switching performance can be improved by serving low-priority packet even though high-priority packet is blocked. In this paper, we study the performance of preemptive priority in an input queueing switch for high speed switch system. The analysis of this switching system is taken into account of the influence of priority scheduling and the window scheme for head-of-line contention. We derive queue length distribution, delay and maximum throughput for the switching system based on these control schemes. Because of the service dependencies between inputs, an exact analysis of this switching system is intractable. Consequently, we provide an approximate analysis based on some independence assumption and the flow conservation rule. We use an equivalent queueing system to estimate the service capability seen by each input. In case of the preemptive priority policy without considering a window scheme, we extend the approximation technique used by Chen and Guerin [1] to obtain more accurate results. Moreover, we also propose newly a window scheme that is appropriate for the preemptive priority switching system in view of implementation and operation. It can improve the total system throughput and delay performance of low priority packets. We also analyze this window scheme using an equivalent queueing system and compare the performance results with that without the window scheme. Numerical results are compared with simulations.
Journal of the Korea Academia-Industrial cooperation Society
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v.20
no.2
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pp.404-411
/
2019
In this study, the HEC-HMS was applied to determine rainfall-runoff processes for the Gokgyuchun basin. Several sub-basins have large-scale reservoirs for agricultural needs and they store large amounts of initial runoff. Three infiltration methods were implemented to reflect the effect of initial loss by reservoirs: 'SCS-CN'(Scheme I), 'SCS-CN' with simple surface method(Scheme II), and 'Initial and Constant rate'(Scheme III). Modeling processes include incorporating three different methods for loss due to infiltration, Clark's UH model for transformation, exponential recession model for baseflow, and Muskingum model for channel routing. The parameters were calibrated using an optimization technique with trial and error method. Performance measures, such as NSE, RAR, and PBIAS, were adopted to aid in the calibration processes. The model performance for those methods was evaluated at Gangcheong station, which is the outlet of study site. Good accuracy in predicting runoff volume and peak flow, and peak time was obtained using the Scheme II and III, considering the initial loss, whereas Scheme I showed low reliability for storms. Scheme III did not show good matches between observed and simulated values for storms with multi peaks. Conclusively, Scheme II provided better results for both single and multi-peak storms. The results of this study can provide a useful tool for decision makers to determine master plans for regional flood control management.
Se Won Oh;Samel Park;Nam-jun Cho;Hyo-Wook Gil;Eun Young Lee;Hyung Geun Oh;Sung-Tae Park
Journal of the Korean Society of Radiology
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v.81
no.4
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pp.912-919
/
2020
Purpose This study aimed to compare the brain perfusion status of patients with chronic kidney disease to a normal control group to identify any significant differences. Materials and Methods The perfusion state of the brain was measured by MRI using the arterial spin labeling technique in 36 patients undergoing hemodialysis due to chronic kidney disease and 36 normal controls. Images were then analyzed in a voxel-wise manner to detect brain areas showing significant perfusion differences between the two groups. Results Patients with chronic kidney disease showed increased perfusion in the form of large clusters across the right fronto-parieto-temporal lobe and the left parieto-occipital lobe. In addition, perfusion increased in the bilateral thalami, midbrain, pons, and cerebellum (p < 0.01, familywise error corrected). Conclusion Brain perfusion appears to increase in patients with chronic kidney disease compared to normal controls. Uremic toxicity is thought to be the cause of this increase as it can cause damage to the microscopic blood vessels and their surrounding structures.
BACKGROUND: Plant factory system of an artificial light type using Light-Emitting Diodes (LEDs), fluorescent light, or metal halide lamp instead of sun light is an ultimated method for plant production without any pesticides regardless of seasonal changes. The plant factory is also completely isolated from outside environmental conditions such as a light, temperature, or humidity compared to conventional greenhouse. Light-environment control such as a quality or quantity in the plant factory system is essential for improving the growth and development of plant species. However, there was little report that the effects of various light qualities provided by LEDs on Ledebouriella seseloides growth under the plant factory system. METHODS AND RESULTS: Ledebouriella seseloides seedlings transplanted at urethane sponge were grown in the plant factory system of a horizontal type with LED artificial lights for 90 days. Yamazaki solution for hydroponic culture of the seedlings was regularly irrigated by the deep flow technique (DFT) system on the culture gutters. Electrical Conductivity (EC) and pH of the solution was recorded at 1.4 ds/m and 5.8 in average, respectively during the experimental period. Number of unfolded leaves, leaf length, shoot fresh and dry weight of the seedlings were three times measured in every 30 days after beginning of the experiment. Blue LEDs, red LEDs, and fluorescent lights inside the plant factory were used as light sources. Conventional fluorescent lamps were considered as a control. In all the treatment, light intensity was maintained at $100{\mu}mol/m^2/s$ on the culture bed. Fresh weight of the seedlings was 3.7 times greater in the treatment with the mixture radiation of fluorescent light and blue+red LEDs (1:3 in energy ratio; Treatment FLBR13) than in fluorescent light treatment (Treatment FL). In FLBR13 treatment, dry weight per seedling was two times greater than in FL or BR11 treatment of blue+red LEDs (1:3 in energy ratio; Treatment BR11) during the culture period. Increasing in number of unfolded leaves was also significantly affected by the FLBR13 treatment comparing with BR11 treatment. CONCLUSION(S): Hydroponic culture of Ledebouriella seseloides seedlings was successfully achieved in the plant factory system with mixture lights of blue, red LEDs and fluorescent lights. Shoot growth of the seedlings was significantly promoted by the FLBR13 with the mixture radiation of fluorescent light, blue, and red LEDs under 1:3 mixture ratio of blue and red LEDs during the experimental period compared to conventional light conditions.
Noh, Seung Won;Park, Jong Seok;Kim, Sung Jin;Kim, Dae-Woong;Kang, Woo Seok
Journal of Bio-Environment Control
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v.29
no.4
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pp.464-472
/
2020
We suggest a hydroponic cultivation system combined with a plasma generator to investigate the changes in the growth and functional substance content of lettuces during the cultivation period. Lettuce seedlings of uniform size were planted in semi-DFT after seeding for 3 weeks, and the plasma-activated water was intermittently operated for 1 hour at an 8 hours cycle for 4 weeks. Lettuces grew with or without plasma-activated water with the nutrient solution in hydroponics culture systems. Among the reactive oxygen species generated during plasma-activated water treatment, brown spots and necrosis appeared in the individuals closer to the plasma generating device due to O3, and there was no significant difference in the growth parameters. While the rutin and total phenolic content of the lettuce shoot grown in the nutrient solution were higher than that of the plasma-activated water, epicatechin contents in plasma-activated water were significantly greater than the nutrient solution. However, in the roots, all kinds of secondary metabolites measured in this work, rutin, epicatechin, quercetin, and total phenolic contents, were significantly higher in the plasma-activated water than the control. These results were indicated that the growth of lettuce was decreased due to the reactive oxygen species such as ozone in the plasma-activated water, but the secondary metabolites in the root zone increased significantly. It has needed to use this technology for the cultivation of root vegetables with the modified plasma-activated water systems to increase secondary metabolite in the roots.
Kim, Won-Gon;Lim, Cheong;Moon, Hyun-Jong;Chun, Eui-Kyung;Chi, Je-Geun;Won, Tae-Hee;Lee, Young-Tak;Chee, Hyun-Keun;Kim, Jun-Woo
Journal of Chest Surgery
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v.30
no.5
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pp.471-478
/
1997
Introduction: The use of rabbits as a cardiopulmonary bypass(CPB) animal model is extremely dif%cult mainly due to technical problems. On the other hand, deep hypothermic circulatory arrest(CA) is used to facilitate surgical repair in a variety of cardiac diseases. Although steroids are generally known to be effective in the treatment of cerebral edema, the protective effects of steroids on the brain during CA are not conclusively established. Objectives of this study are twofold: the establishment of CPB technique in rabbits and the evaluation of preventive effect of steroid on the development of brain edema during CA. Material '||'&'||' Methods: Fifteen New Zealan white rabbits(average body weight 3.5kg) were divided into three experimental groups; control CA group(n=5), CA with Trendelenberg position group(n=5), and CA with Trendelenberg position + steroid(methylprednisolone 30 mglkg) administration group(n=5). After anesthetic induction and tracheostomy, a median sternotomy was performed. An aortic cannula(3.3mm) and a venous ncannula(14 Fr) were inserted, respectively in the ascending aorta and the right atrium. The CPB circuit consisted of a roller pump and a bubble oxygenator. Priming volume of the circuit was approximately 450m1 with 120" 150ml of blood. CPB was initiated at a flow rate of 80~85ml/kg/min, Ten min after the start of CPB, CA was established with duration of 40min at $20^{\circ}C$ of rectal temperature. After CA, CPB was restarted with 20min period of rewarming. Ten min after weaning, the animal was sacrif;cod. One-to-2g portions of the following tissues were rapidly d:ssected and water contents were examined and compared among gr ups: brain, cervical spinal cord, kidney, duodenum, lung, heart, liver, spleen, pancreas. stomach. Statistical significances were analyzed by Kruskal-Wallis nonparametric test. Results: CPB with CA was successfully performed in all cases. Flow rate of 60-100 mlfkgfmin was able to be maintained throughout CPB. During CPB, no significant metabolic acidosis was detected and aortic pressure ranged between 35-55 mmHg. After weaning from CPB, all hearts resumed normal beating spontaneously. There were no statistically significant differences in the water contents of tissues including brain among the three experimental groups. Conclusion: These results indicate (1) CPB can be reliably administered in rabbits if proper technique is used, (2) the effect of steroid on the protection of brain edema related to Trendelenburg position during CA is not established within the scope of this experiment.
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