• 한국전산유체공학회:학술대회논문집
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• 2008.08a
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• pp.43.2-43.2
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• 2008

• Proceedings of the Korean Operations and Management Science Society Conference
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• 1994.04a
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• pp.478-478
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• 1994

• Proceedings of the Membrane Society of Korea Conference
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• 2001.11a
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• pp.146-148
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• 2001

• Journal of Life Science
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• v.19 no.5
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• pp.620-624
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• 2009

D-Ala2-Leu5-enkephalin (DADLE), a hibernation inducer, can induce hibernation-like state in vivo and in vitro. We treated U937 human leukemia cancer cells with DADLE and investigated its possible effect on transcription and proliferation. Treatment of U937 cells with DADLE resulted in growth inhibition and induction of apoptotic cell death on high-dose as measured by MTT assay and DNA flow cytometer analysis. Bcl-XL, c-IAP-2 and survivin genes especially showed decreases in mRNA levels. DADLE treatment also inhibited the levels of cyclooxygenase (COX)-2 mRNA without alteration of COX-1 expression. DNA flow cytometer analysis revealed that DADLE caused arrest of the cell cycle on low-dose, which was associated with a down-regulation of cyclin E at the transcriptional level. DADLE treatment induced a marked down-regulation of cyclin-dependent kinase (Cdk)-2, -4 and -6. In addition, treatment with DADLE decreased telomere associated genes such as, c-myc and TERT, and increased TEP-1 in U937 cells. These results suggest that DADLE can be an inhibition agent in the cell cycle of the human leukemia cancer U937 cell.

• Asian Pacific Journal of Cancer Prevention
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• v.16 no.18
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• pp.8419-8423
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• 2016

Purpose: Anti-cancer activity evaluation of aqueous extract of CRUEL (herbomineral formulation) capsules on renal cell carcinoma cell lines, and exploration of mechanisms of cell death. Materials and Methods: To detect the cytotoxic dose concentration in renal cell carcinoma (RCC) cells, MTT assays were performed and morphological changes after treatment were observed by inverted microscopy. Drug effects against RCC cell lines were assessed with reference to cell cycle distribution (flow cytometry), anti-metastatic potential (wound healing assay) and autophagy(RT-PCR). Results: CRUEL showed anti-proliferative effects against RCC tumor cell lines with an IC50 value of ${\approx}4mg/mL$ in vitro., while inducing cell cycle arrest at S-phase of cell cycle and inhibiting wound healing. LC3 was found to be up-regulated after drug treatment in RT-PCR resulting in an autophagy mode of cell death. Conclusions: This study provides the experimental validation for antitumor activity of CRUEL.

• The Korean Journal of Cytopathology
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• v.8 no.1
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• pp.20-26
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• 1997

The specificity of cytologic examination in effusions is high but the sensitivity is low. Therefore, various ancillary methods for the detection of malignant cells in effusions have been proposed. The presence of an aneuploid cell population is generally considered diagnostic of malignancy. The purpose of this study is to determine whether the routine use of flow cytometry adds to standard cytologic evaluation in effusions. We did flow cytometric DNA analysis in 76 effusions(28 malignant and 48 benign fluids). All the 48 benign effusions were diploid. There were 12(42.9%) aneuploid and 16(67.1%) diploid malignant effusions. Based on these results flow cytometric DNA analysis had a sensitivity of 42.9% and a specificity of 100%. These results suggest that flow cytometric DNA analysis may be a useful adjunct to conventional cytology, but its principal limitation is us relatively low sensitivity.

• Journal of Korean Society for Atmospheric Environment
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• v.19 no.6
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• pp.733-743
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• 2003

The flow field in multiple fluid sphere systems was studied analytically. The expanded zero vorticity cell model based on Kuwabara's theory (1959) was applied and the effects of gas slippage at the collecting surface were considered. Also, the solid sphere system was extended to fluid sphere including the effects of the induced internal circulation inside the liquid droplet spheres or gas bubble systems. As a result, the obtained analytic solution was converged to the existing solutions for flow field around solid and bubble sphere systems with proper boundary conditions. Based on the resolved flow field, the terminal velocity around the collecting fluid spheres was obtained. Subsequently, this study evaluated the most general solution for flow field around the multiple fluid sphere systems. The obtained flow field in multiple fluid sphere could be used as a fundamental consideration of wet scrubber design and devices for removing particles by fluid-fluid interactions.

• Journal of Ocean Engineering and Technology
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• v.6 no.2
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• pp.143-150
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• 1992

Numerical simulation is made of a stably stratified salt-water solution due to lateral heating in an equilateral trapezoidal enclosure using boundary-fitted coordinate in grid generation. Results show four types of the global fluid pattern depending on the buoyancy ratio in the enclosure, i.e., unicell flow pattern for the low buoyancy ratio, layered flow pattern for the increasing buoyancy ratio, layered flow pattern with stagnant zone for the relatively high buoyancy ratio and stagnant flow pattern for the much higher buoyancy ratio.

• 한국신재생에너지학회:학술대회논문집
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• 2006.11a
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• pp.320-323
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• 2006

A numerical study is made of a manifold and bipolar plate in polymer electrolyte fuel cells, the aim of the present study is to describe the characteristics of flow pattern In manifold and bipolar plate. The present work shows that the flow pattern in the bipolar plate is affected by the penetration flow through GDL characterized by clamping pressure and GDL intrusion in to a channel area. Manifold geometry also affects the flow distribution. The recirculation flow by bent duct destroy even distribution In manifold, the present work shows that corner rounding can improve the manifold performance.

• Proceedings of the KSME Conference
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• 2002.08a
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• pp.597-600
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• 2002

In general, the function of intake structure, whether it be a open channel, a fully wetted tunnel, a sump or a tank, is to supply an evenly distributed flow to a pump station. An even distribution of flow, characterized by strong local flow, can result in formation of surface or submerged vortices, and with certain low values of submergence, may introduce air into pump, causing a reduction of capacity and efficiency, an increase in vibration and additional noise. Uneven flow distribution can also increase or decrease the power consumption with a change in total developed head. To avoid these sump problems pump station designers are considered intake structure dimensions, such as approaching upstream, baffle size, sump width, width of pump cell and so on. From this background, flow characteristics of intake within sump are Investigated numerically to obtain the optimal sump design data. The sump model is designed in accordance with HI code.