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http://dx.doi.org/10.5352/JLS.2009.19.5.620

Cell Cycle Arrest by Treatment of D-Ala2-Leu5-enkephalin in Human Leukemia Cancer U937 Cell.  

Lee, Jun-Hyuk (Chemistry and Biotechnology Examination Bureau, Korean Intellectual Property Office)
Choi, Woo-Young (Division of Meridian and Structural Medicine, School of Oriental Medicine. Pusan National University)
Choi, Yung-Hyun (Department of Biochemistry, College of Oriental Medicine, Dongeui University)
Choi, Byung-Tae (Division of Meridian and Structural Medicine, School of Oriental Medicine. Pusan National University)
Publication Information
Journal of Life Science / v.19, no.5, 2009 , pp. 620-624 More about this Journal
Abstract
D-Ala2-Leu5-enkephalin (DADLE), a hibernation inducer, can induce hibernation-like state in vivo and in vitro. We treated U937 human leukemia cancer cells with DADLE and investigated its possible effect on transcription and proliferation. Treatment of U937 cells with DADLE resulted in growth inhibition and induction of apoptotic cell death on high-dose as measured by MTT assay and DNA flow cytometer analysis. Bcl-XL, c-IAP-2 and survivin genes especially showed decreases in mRNA levels. DADLE treatment also inhibited the levels of cyclooxygenase (COX)-2 mRNA without alteration of COX-1 expression. DNA flow cytometer analysis revealed that DADLE caused arrest of the cell cycle on low-dose, which was associated with a down-regulation of cyclin E at the transcriptional level. DADLE treatment induced a marked down-regulation of cyclin-dependent kinase (Cdk)-2, -4 and -6. In addition, treatment with DADLE decreased telomere associated genes such as, c-myc and TERT, and increased TEP-1 in U937 cells. These results suggest that DADLE can be an inhibition agent in the cell cycle of the human leukemia cancer U937 cell.
Keywords
DADLE; U937 cell; cell cycle;
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