Diagnostic Value of Flow Cytometric DNA Analysis in the Evaluation of Effusions

체강삼출액의 진단에 있어서 유세포분석에 의한 DNA 함량 측정의 유용성

  • 이지신 (국군중앙의무시험소 병리과) ;
  • 정상우 (전남의대 병리학교실)
  • Published : 1997.06.30


The specificity of cytologic examination in effusions is high but the sensitivity is low. Therefore, various ancillary methods for the detection of malignant cells in effusions have been proposed. The presence of an aneuploid cell population is generally considered diagnostic of malignancy. The purpose of this study is to determine whether the routine use of flow cytometry adds to standard cytologic evaluation in effusions. We did flow cytometric DNA analysis in 76 effusions(28 malignant and 48 benign fluids). All the 48 benign effusions were diploid. There were 12(42.9%) aneuploid and 16(67.1%) diploid malignant effusions. Based on these results flow cytometric DNA analysis had a sensitivity of 42.9% and a specificity of 100%. These results suggest that flow cytometric DNA analysis may be a useful adjunct to conventional cytology, but its principal limitation is us relatively low sensitivity.